Abstract:
:A delay ( approximately 10 min) in the appearance of intracellular phage is caused by preincubating the infecting phage T4o(1) in proflavine, acridine orange, or ethidium, but not polyamines. No significant delay in attachment is observed. Apparently the presence of the dye is required inside the permeability barrier of the phage at the time of infection. The effect of proflavine is reduced in the presence of polyamines, suggesting that the active site is on DNA. The phage-host complex is sensitive to shear if the infecting phage have been incubated in proflavine or ethidium, indicating that the completion of DNA injection is delayed. Finally no partially injected complexes could be detected after shearing, which suggests that most of the delay occurs near the beginning of the injection process.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Dusenbery DB,Uretz RBdoi
10.1016/S0006-3495(72)86144-7subject
Has Abstractpub_date
1972-08-01 00:00:00pages
1066-72issue
8eissn
0006-3495issn
1542-0086pii
S0006-3495(72)86144-7journal_volume
12pub_type
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更新日期:2000-11-01 00:00:00
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更新日期:1997-01-01 00:00:00
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pub_type: 历史文章,杂志文章
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更新日期:2015-10-06 00:00:00
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更新日期:2010-08-04 00:00:00
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