Effect of structural transition of the host assembly on dynamics of an ion channel peptide: a fluorescence approach.

abstract：:The suprachiasmatic nucleus governs daily variations of physiology and behavior in mammals. Within single neurons, interlocked transcriptional/translational feedback loops generate circadian rhythms on the molecular level. We present a mathematical model that reflects the essential features of the mammalian circadian ...

journal_title：Biophysical journal

authors： Becker-Weimann S,Wolf J,Herzel H,Kramer A

更新日期：2004-11-01 00:00:00

abstract：:The model for carbon monoxide or dioxygen recombination with heme proteins developed by the group at the University of Illinois is reexamined. We propose that the carbon monoxide or dioxygen molecule enters the protein at essentially a diffusion-limited rate determined by the solvent viscosity and that the protein off...

journal_title：Biophysical journal

authors： Austin RH,Chan SS

更新日期：1978-10-01 00:00:00

abstract：:The influence of electrostatic multipole moments up to hexadecapole on the dynamics of photodissociated carbon monoxide (CO) in myoglobin is investigated. The CO electrostatic potential is expressed as an expansion into atomic multipole moments of increasing order up to octopole which are obtained from a distributed m...

journal_title：Biophysical journal

authors： Plattner N,Meuwly M

更新日期：2008-04-01 00:00:00

abstract：:Understanding exposure thresholds for the response of biological systems to extremely low frequency (ELF) electric and magnetic fields is a fundamental problem of long-standing interest. We consider a two-state model for voltage-gated channels in the membrane of an isolated elongated cell (Lcell = 1 mm; rcell = 25 mic...

journal_title：Biophysical journal

authors： Weaver JC,Vaughan TE,Adair RK,Astumian RD

更新日期：1998-11-01 00:00:00

abstract：:The triplet state absorption and phosphorescence of Zn and Pd derivatives of myoglobin were compared. Both metal derivatives exhibit long triplet state lifetimes at room temperature, but whereas the Pd derivative showed exponential decay and an isosbestic point in the transient absorption spectra, the decay of the Zn ...

journal_title：Biophysical journal

authors： Papp S,Vanderkooi JM,Owen CS,Holtom GR,Phillips CM

更新日期：1990-07-01 00:00:00

abstract：:During the first 2 hours of Drosophila development, precisely orchestrated nuclear cleavages, cytoskeletal rearrangements, and directed membrane growth lead to the formation of an epithelial sheet around the yolk. The newly formed epithelium remains relatively quiescent during the next hour as it is patterned by mater...

journal_title：Biophysical journal

authors： Dutta S,Djabrayan NJ,Smits CM,Rowley CW,Shvartsman SY

更新日期：2020-05-19 00:00:00

abstract：:Single epithelial Na+ channels incorporated into planar lipid bilayers were studied to determine the effects of Na concentration on its own conductance. Amiloride-sensitive Na+ channels were obtained from apical membrane vesicles made from A6 cells, a continuous epithelial cell-line derived from amphibian kidney. Sing...

journal_title：Biophysical journal

authors： Olans L,Sariban-Sohraby S,Benos DJ

更新日期：1984-12-01 00:00:00

abstract：:Cofilin/ADF, beryllium fluoride complex (BeFx), and phalloidin have opposing effects on actin filament structure and dynamics. Cofilin/ADF decreases the stability of F-actin by enhancing disorder in subdomain 2, and by severing and accelerating the depolymerization of the filament. BeFx and phalloidin stabilize the su...

journal_title：Biophysical journal

authors： Muhlrad A,Ringel I,Pavlov D,Peyser YM,Reisler E

更新日期：2006-12-15 00:00:00

abstract：:The development of strategies to measure plasma membrane osmotic water permeability (Pf) in epithelial cells has been motivated by the identification of a family of molecular water channels. A general approach utilizing interferometry to measure cell shape and volume was developed and applied to measure Pf in cell lay...

journal_title：Biophysical journal

authors： Farinas J,Verkman AS

更新日期：1996-12-01 00:00:00

abstract：:A mesoscale model of DNA is presented (3SPN.1), extending the scheme previously developed by our group. Each nucleotide is mapped onto three interaction sites. Solvent is accounted for implicitly through a medium-effective dielectric constant and electrostatic interactions are treated at the level of Debye-Hückel theo...

journal_title：Biophysical journal

authors： Sambriski EJ,Schwartz DC,de Pablo JJ

更新日期：2009-03-04 00:00:00

abstract：:The regulated ability of integrin alphaIIbbeta3 to bind fibrinogen plays a crucial role in platelet aggregation and hemostasis. We have developed a model system based on laser tweezers, enabling us to measure specific rupture forces needed to separate single receptor-ligand complexes. First of all, we performed a thor...

journal_title：Biophysical journal

authors： Litvinov RI,Bennett JS,Weisel JW,Shuman H

更新日期：2005-10-01 00:00:00

abstract：:Two-dimensional 1H NMR spectroscopy over a range of temperature through thermal unfolding has been applied to the low-spin, ferric cyanide complex of myoglobin from Aplysia limacina to search for intermediates in the unfolding and to characterize the effect of temperature on the magnetic properties and electronic stru...

journal_title：Biophysical journal

authors： Xia Z,Nguyen BD,Brunori M,Cutruzzolà F,La Mar GN

更新日期：2005-12-01 00:00:00

abstract：:The absence of comprehensive measured kinetic values and the observed inconsistency in the available in vitro kinetic data has hindered the formulation of network-scale kinetic models of biochemical reaction networks. To meet this challenge we present an approach to construct a convex space, termed the k-cone, which c...

journal_title：Biophysical journal

authors： Famili I,Mahadevan R,Palsson BO

更新日期：2005-03-01 00:00:00

abstract：:We have studied the large increase in macroscopic potassium channel current caused by catecholamines in mammalian cardiac cells. An increase in macroscopic K current could result from either an increase in the single-channel current or by an increase in the number of channels that are open. Therefore, we have measured...

journal_title：Biophysical journal

authors： Bennett PB,Kass R,Begenisich T

更新日期：1989-04-01 00:00:00

abstract：:All-atom molecular dynamics simulations of the ion current through OmpF, the major porin in the outer membrane of Escherichia coli, were performed. Starting from the crystal structure, the all-atom modeling allows us to calculate a parameter-free ion conductance in semiquantitative agreement with experiment. Discrepan...

journal_title：Biophysical journal

authors： Pezeshki S,Chimerel C,Bessonov AN,Winterhalter M,Kleinekathöfer U

更新日期：2009-10-07 00:00:00

abstract：:We used micron-sized latex spheres to probe the phase state and the viscoelastic properties of dimyristoylphosphatidylcholine (DMPC) bilayers as a function of temperature. One or two particles were manipulated and stuck to a DMPC giant vesicle by means of an optical trap. Above the fluid-gel main transition temperatur...

journal_title：Biophysical journal

authors： Dimova R,Pouligny B,Dietrich C

更新日期：2000-07-01 00:00:00

abstract：:Evidence that the fluorophore sempervirene binds to nucleic acids is presented. The complexes were studied by fluorescence intensity, spectra, decay lifetime, and polarization methods. Both fluorescent and nonfluorescent complexes are formed. The sempervirene is rigidly fixed to DNA. If ethidium bromide and sempervire...

journal_title：Biophysical journal

authors： Burns VW

更新日期：1974-03-01 00:00:00

abstract：:Erythrocytes were electrofused with multiple rectangular voltage pulses to show an oscillatory movement, divided into swell phases and pump events. During each swell phase, which lasted from 0.5 s to more than 180 s, the fused cells' (doublets') volume increased by colloid osmotic swelling, and the membrane area was e...

journal_title：Biophysical journal

authors： Baumann M

更新日期：1999-11-01 00:00:00

abstract：:Viral fusion peptides are short N-terminal regions of type-1 viral fusion proteins that are critical for virus entry. Although the importance of viral fusion peptides in virus-cell membrane fusion is established, little is known about how they function. We report the effects of wild-type (WT) hemagglutinin (HA) fusion...

journal_title：Biophysical journal

authors： Chakraborty H,Tarafdar PK,Klapper DG,Lentz BR

更新日期：2013-12-03 00:00:00

abstract：:Late-onset heart failure (HF) is a known side effect of doxorubicin chemotherapy. Typically, patients are diagnosed when already at an irreversible stage of HF, which allows few or no treatment options. Identifying the causes of compromised cardiac function in this patient group may improve early patient diagnosis and...

journal_title：Biophysical journal

authors： Lewalle A,Land S,Merken JJ,Raafs A,Sepúlveda P,Heymans S,Kleinjans J,Niederer SA

更新日期：2019-12-17 00:00:00

abstract：:Using a model for catalysis of a dynamic equilibrium, the role of constraint in catalysis is quantified. The intrinsic rigidity of proteins is shown to be insufficient to constrain the activated complexes of enzymes, irrespective of the mechanism. However, when minimization of the surface excess free energy of water s...

journal_title：Biophysical journal

authors： Vanselow DG

更新日期：2002-05-01 00:00:00

abstract：:Thermal inactivation of T(4) and lambda bacteriophage shows two components of differing sensitivity are present. These cannot be interpreted as owing to nucleic acid and protein. One protein function-the inhibition of radiation-induced DNA degradation-is lost with quite different thermal kinetics. lambda heated in the...

journal_title：Biophysical journal

authors： Pollard EC,Solosko W

更新日期：1971-01-01 00:00:00

abstract：:Voltage-dependent G-protein inhibition of N-type calcium channels reduces presynaptic calcium entry, sharply attenuating neurotransmitter release. Studies in neurons demonstrate that G-proteins have multiple modulatory effects on N-type channels. The observed changes may reflect genuine complexity in G-protein action ...

journal_title：Biophysical journal

authors： Patil PG,de Leon M,Reed RR,Dubel S,Snutch TP,Yue DT

更新日期：1996-11-01 00:00:00

abstract：:We present a theoretical analysis of intracellular calcium waves propagated by calcium feedback at the inositol 1,4,5-trisphosphate (IP3) receptor. The model includes essential features of calcium excitability, but is still analytically tractable. Formulas are derived for the wave speed, amplitude, and width. The calc...

journal_title：Biophysical journal

authors： Kupferman R,Mitra PP,Hohenberg PC,Wang SS

更新日期：1997-06-01 00:00:00

abstract：:In this study stereochemically pure d-erythro-sphingomyelins (SMs) with either 16:0 or 18:1(cisDelta9) as the N-linked acyl-chain were synthesized. Our purpose was to examine the properties of these sphingomyelins and acyl-chain matched racemic (d-erythro/l-threo) sphingomyelins in model membranes. Liquid-expanded d-e...

journal_title：Biophysical journal

authors： Ramstedt B,Slotte JP

更新日期：1999-09-01 00:00:00

abstract：:The interactions of the hydrophobic pulmonary surfactant proteins SP-B and SP-C with 1,2-dipalmitoylphosphatidylcholine in mixed, spread monolayer films have been studied in situ at the air/water interface with the technique of external reflection absorption infrared spectroscopy (IRRAS). SP-C has a mostly alpha-helic...

journal_title：Biophysical journal

authors： Pastrana-Rios B,Taneva S,Keough KM,Mautone AJ,Mendelsohn R

更新日期：1995-12-01 00:00:00

abstract：:The mating pathway in Saccharomyces cerevisiae is one of the best understood signal transduction pathways in eukaryotes. It transmits the mating signal from plasma membrane into the nucleus through the G-protein coupled receptor and the mitogen-activated protein kinase (MAPK) cascade. According to current understandin...

journal_title：Biophysical journal

authors： Shao D,Zheng W,Qiu W,Ouyang Q,Tang C

更新日期：2006-12-01 00:00:00

abstract：:BP100 (KKLFKKILKYL-NH(2)) is a short cecropin A-melittin hybrid peptide, obtained through a combinatorial chemistry approach, which is highly effective in inhibiting both the in vitro and in vivo growth of economically important plant pathogenic Gram-negatives. The intrinsic Tyr fluorescence of BP100 was taken advanta...

journal_title：Biophysical journal

authors： Ferre R,Melo MN,Correia AD,Feliu L,Bardají E,Planas M,Castanho M

更新日期：2009-03-04 00:00:00

abstract：:In an attempt to quantitate the physical behavior of biological systems, Fourier analysis has been applied to the respiratory and circulatory systems by a number of investigators. The validity of this application has been questioned on the basis that these systems are nonlinear and not strictly periodic. If these obje...

journal_title：Biophysical journal

authors： Attinger EO,Anné A,McDonald DA

更新日期：1966-05-01 00:00:00

abstract：:Many different cell types are able to migrate by formation of a thin actin-based cytoskeletal extension. Recently, it became evident that this extension consists of two distinct substructures, designated lamellipodium and lamellum, which differ significantly in their kinetic and kinematic properties as well as their b...

journal_title：Biophysical journal

authors： Huber F,Käs J,Stuhrmann B

更新日期：2008-12-15 00:00:00