Abstract:
:In this study maximum shortening velocity (Vmax) and isometric tension (P0) in skinned single fibers from rat slow soleus (SOL) and fast superficial vastus lateralis (SVL) muscles were examined after varying degrees of filament lattice compression with dextran. In both fiber types Vmax was greatest in the absence of dextran and decreased as the concentration of dextran was increased between 2.5 and 10 g/100 ml. At 10% dextran, which compressed fiber width by 31-38%, Vmax relative to the initial 0% dextran value was 0.28 +/- 0.03 (mean +/- SE) and 0.26 +/- 0.02 in SVL and SOL fibers, respectively. The effect of compression to depress Vmax was reversed completely by returning the fiber to 0% dextran. The force-generating capability of skinned fibers was not as sensitive to variations in cell width. In both the SOL and SVL fibers P0 increased by 3-7% when the concentration of dextran was increased from 0 to 5%. Further compression of lattice volume with 10% dextran resulted in a 8-13% decline in P0 relative to the initial value. While the precise mechanism by which filament lattice spacing modulates contractile function is not known, our results suggest that the major effect is upon the rate constant for cross-bridge detachment.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Metzger JM,Moss RLdoi
10.1016/S0006-3495(87)83197-1subject
Has Abstractpub_date
1987-07-01 00:00:00pages
127-31issue
1eissn
0006-3495issn
1542-0086pii
S0006-3495(87)83197-1journal_volume
52pub_type
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