Cysteines in the Shaker K+ channel are not essential for channel activity or zinc modulation.

Abstract:

:We investigated whether the cysteine residues in Shaker potassium (K+) channels are essential for activation and inactivation gating or for modulation of activation gating by external zinc (Zn2+). Mutants of the Shaker K+ channel were prepared in which all seven cysteine residues were replaced (C-less). These changes were made in both wild-type Shaker H4 channels and in a deletion mutant (delta 6-46) lacking N-type ("fast") inactivation. Replacement of all cysteines left most functional properties of the K+ currents unaltered. The most noticeable difference between the C-less and wild-type currents was the faster C-type inactivation of the C-less channel which could be attributed largely to the mutation of Cys462. This is consistent with the effects of previously reported mutations of nearby residues in the S6 region. There were also small changes in the activation gating of C-less currents. Modulation by external Zn2+ of the voltage dependence and rate of activation gating is preserved in the C-less channels, indicating that none of the cysteines in the Shaker K+ channel plays an important role in Zn2+ modulation.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Boland LM,Jurman ME,Yellen G

doi

10.1016/s0006-3495(94)80843-4

subject

Has Abstract

pub_date

1994-03-01 00:00:00

pages

694-9

issue

3 Pt 1

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(94)80843-4

journal_volume

66

pub_type

杂志文章
  • NaCl reflection coefficients in proximal tubule apical and basolateral membrane vesicles. Measurement by induced osmosis and solvent drag.

    abstract::Two independent methods, induced osmosis and solvent drag, were used to determine the reflection coefficients for NaCl (sigma NaCl) in brush border and basolateral membrane vesicles isolated from rabbit proximal tubule. In the induced osmosis method, vesicles loaded with sucrose were subjected to varying inward NaCl g...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(89)82920-0

    authors: Pearce D,Verkman AS

    更新日期:1989-06-01 00:00:00

  • Analytical description of the activation of multi-state receptors by continuous neurotransmitter signals at brain synapses.

    abstract::Chemical synaptic transmission is a fundamental component of interneuronal communications in the central nervous system (CNS). Discharge of a presynaptic vesicle containing a few thousand molecules (a quantum) of neurotransmitter into the synaptic cleft generates a transmitter concentration signal that drives postsyna...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(97)78761-7

    authors: Uteshev VV,Pennefather PS

    更新日期:1997-03-01 00:00:00

  • Protein-protein interactions in calcium transport regulation probed by saturation transfer electron paramagnetic resonance.

    abstract::We have used electron paramagnetic resonance (EPR) to probe the homo- and heterooligomeric interactions of reconstituted sarcoplasmic reticulum Ca-ATPase (SERCA) and its regulator phospholamban (PLB). SERCA is responsible for restoring calcium to the sarcoplasmic reticulum to allow muscle relaxation, whereas PLB inhib...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2012.08.032

    authors: James ZM,McCaffrey JE,Torgersen KD,Karim CB,Thomas DD

    更新日期:2012-09-19 00:00:00

  • Reversible surface aggregation in pore formation by pardaxin.

    abstract::The mechanism of leakage induced by surface active peptides is not yet fully understood. To gain insight into the molecular events underlying this process, the leakage induced by the peptide pardaxin from phosphatidylcholine/ phosphatidylserine/cholesterol large unilamellar vesicles was studied by monitoring the rate ...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(96)79822-3

    authors: Rapaport D,Peled R,Nir S,Shai Y

    更新日期:1996-06-01 00:00:00

  • Kinetic studies of calcium-induced calcium release in cardiac sarcoplasmic reticulum vesicles.

    abstract::Fast Ca(2+) release kinetics were measured in cardiac sarcoplasmic reticulum vesicles actively loaded with Ca(2+). Release was induced in solutions containing 1.2 mM free ATP and variable free [Ca(2+)] and [Mg(2+)]. Release rate constants (k) were 10-fold higher at pCa 6 than at pCa 5 whereas Ryanodine binding was hig...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(03)75037-1

    authors: Sánchez G,Hidalgo C,Donoso P

    更新日期:2003-04-01 00:00:00

  • Interrogating biology with force: single molecule high-resolution measurements with optical tweezers.

    abstract::Single molecule force spectroscopy methods, such as optical and magnetic tweezers and atomic force microscopy, have opened up the possibility to study biological processes regulated by force, dynamics of structural conformations of proteins and nucleic acids, and load-dependent kinetics of molecular interactions. Amon...

    journal_title:Biophysical journal

    pub_type: 杂志文章,评审

    doi:10.1016/j.bpj.2013.08.007

    authors: Capitanio M,Pavone FS

    更新日期:2013-09-17 00:00:00

  • Analysis of the anisotropy decay of trans-parinaric acid in lipid bilayers.

    abstract::An analysis is presented of the complex anisotropy behavior of trans-parinaric acid in single component DEPC lipid bilayers. It is shown that a model involving two species with distinct lifetime and motional behavior is required, and is adequate, to explain the observed data. In particular, the observed increase in th...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(89)82909-1

    authors: Ruggiero A,Hudson B

    更新日期:1989-06-01 00:00:00

  • Enantiomeric effects on excitation-contraction coupling in frog skeletal muscle by a chiral phenoxy carboxylic acid.

    abstract::Aromatic monocarboxylic acids are known to significantly potentiate the mechanical response of skeletal muscle fibers. In this study we investigated the effects of enantiomers of 2-(4-chlorophenoxy)propionic acid, chemically one of the simplest aromatic monocarboxylic acids with chiral properties, on mechanical thresh...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(90)82515-7

    authors: Heiny JA,Jong D,Bryant SH,Conte-Camerino D,Tortorella V

    更新日期:1990-01-01 00:00:00

  • Imaging the traction stresses exerted by locomoting cells with the elastic substratum method.

    abstract::The cytoskeletal activity of motile or adherent cells is frequently seen to induce detectable displacements of sufficiently compliant substrata. The physics of this phenomenon is discussed in terms of the classical theory of small-strain, plane-stress elasticity. The main results of such analysis is a transform expres...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(96)79767-9

    authors: Dembo M,Oliver T,Ishihara A,Jacobson K

    更新日期:1996-04-01 00:00:00

  • Origin of twist-bend coupling in actin filaments.

    abstract::Actin filaments are semiflexible polymers that display large-scale conformational twisting and bending motions. Modulation of filament bending and twisting dynamics has been linked to regulatory actin-binding protein function, filament assembly and fragmentation, and overall cell motility. The relationship between act...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2010.07.009

    authors: De La Cruz EM,Roland J,McCullough BR,Blanchoin L,Martiel JL

    更新日期:2010-09-22 00:00:00

  • Single chromatin fiber stretching reveals physically distinct populations of disassembly events.

    abstract::Eukaryotic DNA is packaged into the cell nucleus as a nucleoprotein complex, chromatin. Despite this condensed state, access to the DNA sequence must occur during gene expression and other essential genetic events. Here we employ optical tweezers stretching of reconstituted chromatin fibers to investigate the release ...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1529/biophysj.104.053074

    authors: Pope LH,Bennink ML,van Leijenhorst-Groener KA,Nikova D,Greve J,Marko JF

    更新日期:2005-05-01 00:00:00

  • High-resolution probing of local conformational changes in proteins by the use of multiple labeling: unfolding and self-assembly of human carbonic anhydrase II monitored by spin, fluorescent, and chemical reactivity probes.

    abstract::Two different spin labels, N-(1-oxyl-2,2,5,5-tetramethyl-3-pyrrolidinyl)iodoacetamide (IPSL) and (1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl) methanethiosulfonate (MTSSL), and two different fluorescent labels 5-((((2-iodoacetyl)amino)-ethyl)amino)naphtalene-1-sulfonic acid (IAEDANS) and 6-bromoacetyl-2-dimetylaminon...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(01)76253-4

    authors: Hammarström P,Owenius R,Mårtensson LG,Carlsson U,Lindgren M

    更新日期:2001-06-01 00:00:00

  • What Makes a Good Pore Former: A Study of Synthetic Melittin Derivatives.

    abstract::Pore formation by membrane-active peptides, naturally encountered in innate immunity and infection, could have important medical and technological applications. Recently, the well-studied lytic peptide melittin has formed the basis for the development of combinatorial libraries from which potent pore-forming peptides ...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2020.02.024

    authors: Sepehri A,PeBenito L,Pino-Angeles A,Lazaridis T

    更新日期:2020-04-21 00:00:00

  • Temperature modulation of integrin-mediated cell adhesion.

    abstract::In response to external stimuli, cells modulate their adhesive state by regulating the number and intrinsic affinity of receptor/ligand bonds. A number of studies have shown that cell adhesion is dramatically reduced at room or lower temperatures as compared with physiological temperature. However, the underlying mech...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2010.06.037

    authors: Rico F,Chu C,Abdulreda MH,Qin Y,Moy VT

    更新日期:2010-09-08 00:00:00

  • Fluorescence energy transfer reveals microdomain formation at physiological temperatures in lipid mixtures modeling the outer leaflet of the plasma membrane.

    abstract::An approach is described using fluorescence resonance energy transfer (FRET) to detect inhomogeneity in lipid organization, on distance scales of the order of tens of nanometers or greater, in lipid bilayers. This approach compares the efficiency of energy transfer between two matched fluorescent lipid donors, differi...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(03)74542-1

    authors: Silvius JR

    更新日期:2003-08-01 00:00:00

  • Influence of counter-ions on the crystal structures of DNA decamers: binding of [Co(NH3)6]3+ and Ba2+ to A-DNA.

    abstract::A-DNA is a stable alternative right-handed double helix that is favored by certain sequences (e.g., (dG)n.(dC)n) or under low humidity conditions. Earlier A-DNA structures of several DNA oligonucleotides and RNA.DNA chimeras have revealed some conformational variation that may be the result of sequence-dependent effec...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(95)79929-5

    authors: Gao YG,Robinson H,van Boom JH,Wang AH

    更新日期:1995-08-01 00:00:00

  • Inactivation of L-type Ca channels in embryonic chick ventricle cells: dependence on the cytoskeletal agents colchicine and taxol.

    abstract::This article shows that colchicine and taxol strongly influence the kinetics of L-type Ca channels in intact cardiac cells, and it suggests a mechanism for this action. It is known that colchicine disassociates microtubules into tubulin, and that taxol stabilizes microtubules. We have found that colchicine increases t...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(94)80715-5

    authors: Galli A,DeFelice LJ

    更新日期:1994-12-01 00:00:00

  • Characterization of a range of fura dyes with two-photon excitation.

    abstract::Two-photon excitation (TPE) spectra of Fura-2, -4F, -6F, -FF, and Furaptra were characterized using a tunable (750-850 nM) ultra-short pulse laser. Two-photon fluorescence of these dyes was studied in free solution and in the cytosol of isolated rabbit ventricular cardiomyocytes. The TPE spectra of the Ca(2+)-free and...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(04)74241-1

    authors: Wokosin DL,Loughrey CM,Smith GL

    更新日期:2004-03-01 00:00:00

  • Codon-dependent tRNA fluctuations monitored with fluorescence polarization.

    abstract::During protein synthesis dictated by the codon sequence of messenger RNA, the ribosome selects aminoacyl-tRNA (aa-tRNA) with high accuracy, the exact mechanism of which remains elusive. By using a single-molecule fluorescence resonance energy transfer method coupled with fluorescence emission anisotropy, we provide ev...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2010.10.026

    authors: Mishra PP,Qureshi MT,Ren W,Lee TH

    更新日期:2010-12-01 00:00:00

  • Actin's view of actomyosin interface.

    abstract::Actomyosin interactions were examined by using yeast actin mutants with alanines replacing charged amino acid pairs D24/D25, E99/E100, D80/D81, and E83/K84. In the in vitro motility experiments, actin filaments of D24A/D25A or E99A/E100A mutants moved in the presence of 0.7% methylcellulose at the velocities of wild-t...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:

    authors: Miller CJ,Cheung P,White P,Reisler E

    更新日期:1995-04-01 00:00:00

  • Structural significance of the plasma membrane calcium pump oligomerization.

    abstract::The oligomerization of the plasma membrane calcium pump (PMCA) in phospholipid/detergent micelles was evaluated using a combined spectroscopic and kinetic approach and related to the enzyme stability. Energy transfer between fluorescein-5'-isothiocyanate and eosin-5'-isothiocyanate attached to different PMCA molecules...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/s0006-3495(02)75408-8

    authors: Levi V,Rossi JP,Castello PR,González Flecha FL

    更新日期:2002-01-01 00:00:00

  • Binding of alkaline cations to the double-helical form of gramicidin.

    abstract::Gramicidin is a polypeptide antibiotic that forms monovalent cation-specific channels in membrane environments. In organic solvents and in lipids containing unsaturated fatty acid chains, it forms a double-helical "pore" structure, in which two monomers are intertwined. This form of gramicidin can bind two cations ins...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(96)79213-5

    authors: Chen Y,Wallace BA

    更新日期:1996-07-01 00:00:00

  • Stationary and nonstationary correlation-frequency analysis of heterodyne mode laser light scattering: magnitude and periodicity of canine tracheal ciliary beat frequency in vivo.

    abstract::Stationary and nonstationary correlation-frequency analysis of heterodyne laser light scattering were utilized to make automated, on-line, objective measurements of tracheal ciliary beat frequency (CBF) in intact, anesthetized canines. The stationary correlation-frequency analysis laser light-scattering technique was ...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/s0006-3495(94)80864-1

    authors: Chandra T,Yeates DB,Miller IF,Wong LB

    更新日期:1994-03-01 00:00:00

  • Phase behavior of an intact monoclonal antibody.

    abstract::Understanding protein phase behavior is important for purification, storage, and stable formulation of protein drugs in the biopharmaceutical industry. Glycoproteins, such as monoclonal antibodies (MAbs) are the most abundant biopharmaceuticals and probably the most difficult to crystallize among water-soluble protein...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1529/biophysj.106.098293

    authors: Ahamed T,Esteban BN,Ottens M,van Dedem GW,van der Wielen LA,Bisschops MA,Lee A,Pham C,Thömmes J

    更新日期:2007-07-15 00:00:00

  • The state of water in muscle tissue as determined by proton nuclear magnetic resonance.

    abstract::The nuclear magnetic resonance (NMR) of water protons in live and glycerinated muscle, suspensions of glycerinated myofibrils, and solutions of several muscle proteins has been studied. T(1) and T(2), measured on partially hydrated proteins by pulsed spin-echo techniques, decreased as the ratio of water to protein dec...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/S0006-3495(71)86274-4

    authors: Cooke R,Wien R

    更新日期:1971-12-01 00:00:00

  • Mathematical analysis and quantification of fluorescent proteins as transcriptional reporters.

    abstract::Fluorescent proteins are often used as reporters of transcriptional activity. Here we present a mathematical characterization of a novel fluorescent reporter that was recently engineered to have a short half-life (approximately 12 min). The advantage of this destabilized protein is that it can track the transient tran...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1529/biophysj.107.122200

    authors: Wang X,Errede B,Elston TC

    更新日期:2008-03-15 00:00:00

  • Association of Endophilin B1 with Cytoplasmic Vesicles.

    abstract::Endophilins are SH3- and BAR domain-containing proteins implicated in membrane remodeling and vesicle formation. Endophilins A1 and A2 promote the budding of endocytic vesicles from the plasma membrane, whereas endophilin B1 has been implicated in vesicle budding from intracellular organelles, including the trans-Golg...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2016.06.017

    authors: Li J,Barylko B,Eichorst JP,Mueller JD,Albanesi JP,Chen Y

    更新日期:2016-08-09 00:00:00

  • Calcium and stretch activation modulate power generation in Drosophila flight muscle.

    abstract::Many animals regulate power generation for locomotion by varying the number of muscle fibers used for movement. However, insects with asynchronous flight muscles may regulate the power required for flight by varying the calcium concentration ([Ca(2+)]). In vivo myoplasmic calcium levels in Drosophila flight muscle hav...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2011.09.034

    authors: Wang Q,Zhao C,Swank DM

    更新日期:2011-11-02 00:00:00

  • The Roles of Microtubules and Membrane Tension in Axonal Beading, Retraction, and Atrophy.

    abstract::Axonal beading, or the formation of a series of swellings along the axon, and retraction are commonly observed shape transformations that precede axonal atrophy in Alzheimer's disease, Parkinson's disease, and other neurodegenerative conditions. The mechanisms driving these morphological transformations are poorly und...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2019.07.046

    authors: Datar A,Ameeramja J,Bhat A,Srivastava R,Mishra A,Bernal R,Prost J,Callan-Jones A,Pullarkat PA

    更新日期:2019-09-03 00:00:00

  • Mechanical Characteristics of Ultrafast Zebrafish Larval Swimming Muscles.

    abstract::Zebrafish (Danio rerio) swim within days of fertilization, powered by muscles of the axial myotomes. Forces generated by these muscles can be measured rapidly in whole, intact larval tails by adapting protocols developed for ex vivo muscle mechanics. But it is not known how well these measurements reflect the function...

    journal_title:Biophysical journal

    pub_type: 杂志文章

    doi:10.1016/j.bpj.2020.06.036

    authors: Mead AF,Kennedy GG,Palmer BM,Ebert AM,Warshaw DM

    更新日期:2020-08-18 00:00:00