Membrane on a chip: a functional tethered lipid bilayer membrane on silicon oxide surfaces.

abstract：:The relevance of various residue positions for the stability and the folding characteristics of the prion protein in its normal cellular form are investigated by using molecular dynamics simulations of models exploiting the topology of the native state. These models allow for reproducing the experimentally validated t...

journal_title：Biophysical journal

authors： Settanni G,Hoang TX,Micheletti C,Maritan A

更新日期：2002-12-01 00:00:00

abstract：:VP16, a protein encoded by herpes simplex virus, has a well-characterized 78 amino acid acidic activation domain. When tethered to DNA, tandem repeats of an eight amino acid motif taken from this region stimulate the transcription of a nearby gene. This work addresses how these minimal activation motifs interact with ...

journal_title：Biophysical journal

authors： Bamdad C

更新日期：1998-10-01 00:00:00

abstract：:We present a combined experimental and computational study of the bundling of F-actin filaments induced by lysozyme proteins. Synchrotron small-angle x-ray scattering results show that these bundles consist of close-packed columnar complexes in which the actin is held together by incommensurate, one-dimensional arrays...

journal_title：Biophysical journal

authors： Guáqueta C,Sanders LK,Wong GC,Luijten E

更新日期：2006-06-15 00:00:00

abstract：:In the absence of atomic structures of high-density lipoproteins in their lipid-bound states, many molecular models have been produced based on experimental data. Using molecular dynamics, we show that a recently proposed "solar-flares" model of discoidal high-density lipoprotein is implausible. Our simulations show a...

journal_title：Biophysical journal

authors： Shih AY,Sligar SG,Schulten K

更新日期：2008-06-01 00:00:00

abstract：:The cellular cytoplasm is a complex, heterogeneous environment (both spatially and temporally) that exhibits viscoelastic behavior. To further develop our quantitative insight into cellular transport, we analyze data sets of mRNA molecules fluorescently labeled with MS2-GFP tracked in real time in live Escherichia col...

journal_title：Biophysical journal

authors： Lampo TJ,Stylianidou S,Backlund MP,Wiggins PA,Spakowitz AJ

更新日期：2017-02-07 00:00:00

abstract：:Impact loading of articular cartilage causes extensive chondrocyte death. Cell membranes have a limited elastic range of 3-4% strain but are protected from direct stretch during physiological loading by their membrane reservoir, an intricate pattern of membrane folds. Using a finite-element model, we suggested previou...

journal_title：Biophysical journal

authors： Moo EK,Amrein M,Epstein M,Duvall M,Abu Osman NA,Pingguan-Murphy B,Herzog W

更新日期：2013-10-01 00:00:00

abstract：:We used time-resolved Förster resonance energy transfer, circular dichroism, and molecular dynamics simulation to investigate the structural dependence of synaptotagmin 1's intrinsically disordered region (IDR) on phosphorylation and dielectric constant. We found that a peptide corresponding to the full-length IDR seq...

journal_title：Biophysical journal

authors： Fealey ME,Binder BP,Uversky VN,Hinderliter A,Thomas DD

更新日期：2018-02-06 00:00:00

abstract：:Members of the spectrin superfamily of proteins contain different numbers of homologous repeats arranged in tandem. Each of these consists of a three-alpha-helix motif, comprising two similarly and one oppositely directed alpha-helical segment joined by nonhelical linkers of characteristic length. The right-handed alp...

journal_title：Biophysical journal

authors： Parry DA,Dixon TW,Cohen C

更新日期：1992-04-01 00:00:00

abstract：:Cell proliferation and contact inhibition play a major role in maintaining epithelial cell homeostasis. Prior experiments have shown that externally applied forces, such as stretch, result in increased proliferation in an E-cadherin force-dependent manner. In this study, the spatial regulation of cell proliferation in...

journal_title：Biophysical journal

authors： Mohan A,Schlue KT,Kniffin AF,Mayer CR,Duke AA,Narayanan V,Arsenovic PT,Bathula K,Danielsson BE,Dumbali SP,Maruthamuthu V,Conway DE

更新日期：2018-09-04 00:00:00

abstract：:Interpretation of fluorescence anisotropy decay for the case of restricted rotational diffusion often requires a model. To investigate the extent of model dependence, two models are compared: a strict cone model, in which a fluorescent probe wobbles uniformly within a cone, and a Gaussian model, where the stationary d...

journal_title：Biophysical journal

authors： Kinosita K Jr,Ikegami A,Kawato S

更新日期：1982-02-01 00:00:00

abstract：:We have used identified neurons from the abdominal ganglion of the mollusc Aplysia to construct and analyze two circuits in vitro. Each of these circuits was capable of producing two patterns of persistent activity; that is, they had bistable output states. The output could be switched between the stable states by a b...

journal_title：Biophysical journal

authors： Kleinfeld D,Raccuia-Behling F,Chiel HJ

更新日期：1990-04-01 00:00:00

abstract：:Sphingomyelin is an abundant lipid in some cellular membrane domains, such as lipid rafts. Hydrogen bonding and hydrophobic interactions of the lipid with surrounding components such as neighboring sphingomyelin and cholesterol (Cho) are widely considered to stabilize the raft-like liquid-ordered (Lo) domains in membr...

journal_title：Biophysical journal

authors： Yano Y,Hanashima S,Yasuda T,Tsuchikawa H,Matsumori N,Kinoshita M,Al Sazzad MA,Slotte JP,Murata M

更新日期：2018-10-16 00:00:00

abstract：:Over the last decade, functionally designed DNA nanostructures applied to lipid membranes prompted important achievements in the fields of biophysics and synthetic biology. Taking advantage of the universal rules for self-assembly of complementary oligonucleotides, DNA has proven to be an extremely versatile biocompat...

journal_title：Biophysical journal

authors： Czogalla A,Franquelim HG,Schwille P

更新日期：2016-04-26 00:00:00

abstract：:Previous studies on aurein 2.2 and 2.3 in DMPC/DMPG and POPC/POPG membranes have shown that bilayer thickness and phosphatidylglycerol content have a significant impact on the interaction of these peptides with membrane bilayers. Further examination with the DiSC(3)5 assay has indicated that aurein 2.2 induces greater...

journal_title：Biophysical journal

authors： Cheng JT,Hale JD,Kindrachuk J,Jenssen H,Elliott M,Hancock RE,Straus SK

更新日期：2010-11-03 00:00:00

abstract：:Xenopus oocytes express mechanosensitive (MS(XO)) channels that can be studied in excised patches of membrane with the patch-clamp technique. This study examines the steady-state kinetic gating properties of MS(XO) channels using detailed single-channel analysis. The open and closed one-dimensional dwell-time distribu...

journal_title：Biophysical journal

authors： Gil Z,Magleby KL,Silberberg SD

更新日期：2001-10-01 00:00:00

abstract：:Nonequilibrium molecular dynamics (NEMD) computer simulations are used to calculated the bulk modulus for a dimyristoylphosphatidylcholine bilayer. A methodology is developed whereby NEMD can be effectively used to calculate material properties for complex systems that undergo long time-scale conformational changes. I...

journal_title：Biophysical journal

authors： Ayton G,Smondyrev AM,Bardenhagen SG,McMurtry P,Voth GA

更新日期：2002-03-01 00:00:00

abstract：:Dynamic properties of class A beta-lactamase TEM-1 are investigated from molecular dynamics (MD) simulations. Comparison of MD-derived order parameters with those obtained from model-free analysis of nuclear magnetic resonance (NMR) relaxation data shows high agreement for N-H moieties within alpha- and beta-secondary...

journal_title：Biophysical journal

authors： Fisette O,Morin S,Savard PY,Lagüe P,Gagné SM

更新日期：2010-02-17 00:00:00

abstract：:It has been hypothesized, from earlier studies on single-tether extraction from individual leukocytes and human umbilical vein endothelial cells, that during rolling of leukocytes on the endothelium, simultaneous extraction of membrane nanotubes (tethers) occurs, resulting in enhancement of the force decrease on the a...

journal_title：Biophysical journal

authors： Girdhar G,Shao JY

更新日期：2007-12-01 00:00:00

abstract：:By combining the osmotic stress technique with small-angle x-ray scattering measurements, we followed the structural response of the casein micelle to an overall increase in concentration. When the aqueous phase that separates the micelles is extracted, they behave as polydisperse repelling spheres and their internal ...

journal_title：Biophysical journal

authors： Bouchoux A,Gésan-Guiziou G,Pérez J,Cabane B

更新日期：2010-12-01 00:00:00

abstract：:Intracellular cargo transport frequently involves multiple motor types, either having opposite directionality or having the same directionality but different speeds. Although significant progress has been made in characterizing kinesin motors at the single-molecule level, predicting their ensemble behavior is challeng...

journal_title：Biophysical journal

authors： Arpağ G,Shastry S,Hancock WO,Tüzel E

更新日期：2014-10-21 00:00:00

abstract：:We have performed molecular dynamics simulations of multiple copies of the lung-surfactant peptide SP-B1-25 in a palmitic acid (PA) monolayer. SP-B1-25 is a shorter version of lung-surfactant protein B, an important component of lung surfactant. Up to 30 ns simulations of 20 wt % SP-B1-25 in the PA monolayers were per...

journal_title：Biophysical journal

authors： Lee H,Kandasamy SK,Larson RG

更新日期：2005-12-01 00:00:00

abstract：:Starzak and Starzak (1978. IEEE Trans. Biomed. Eng. 25:201-204.) proposed that, in cyclic application of current and voltage-clamps, the fidelity of the match of the output current with the original stimulus could be used to measure the spatial uniformity of voltage in a membrane. However, they failed to find such a m...

journal_title：Biophysical journal

authors： Moore JW,Stockbridge N,Schiff SJ

更新日期：1982-12-01 00:00:00

abstract：:The interaction between actin and myosin in the filament array of glycerinated muscle fibers has been monitored using paramagnetic probes and mechanical measurements. Both fiber stiffness and the spectra of probes bound to a reactive sulfydral on the myosin head were measured as the actomyosin bond was weakened by add...

journal_title：Biophysical journal

authors： Pate E,Cooke R

更新日期：1988-04-01 00:00:00

abstract：:Structural changes in frog skeletal muscle were studied using x-ray diffraction with a time resolution of 0.53-1.02 ms after a single electrical stimulus at 8 degrees C. Tension began to drop at 6 ms (latency relaxation), reached a minimum at 8 ms, and then twitch tension developed. The intensity of the meridional ref...

journal_title：Biophysical journal

authors： Yagi N

更新日期：2003-02-01 00:00:00

abstract：:Phospholipids have been treated as dimers on a hexagonal lattice, and a move has been introduced that allows the dimers to move and change their orientation on the lattice. Simulations have been performed in which phospholipid chains have been treated as being either independent or infinitely coupled thermodynamically...

journal_title：Biophysical journal

authors： Jerala R,Almeida PF,Biltonen RL

更新日期：1996-08-01 00:00:00

abstract：:Investigation of the molecular mechanisms underlying amyloid-related human diseases attracts close attention. These diseases, the number of which currently is above 40, are characterized by formation of peptide or protein aggregates containing a cross-β structure. Most of the amyloidogenesis mechanisms described so fa...

journal_title：Biophysical journal

authors： Katina NS,Balobanov VA,Ilyina NB,Vasiliev VD,Marchenkov VV,Glukhov AS,Nikulin AD,Bychkova VE

更新日期：2017-09-05 00:00:00

abstract：:The inositol (1,4,5)-trisphosphate receptor (InsP(3)R) is an intracellular calcium (Ca(2+)) release channel that plays a crucial role in cell signaling. In Drosophila melanogaster a single InsP(3)R gene (itpr) encodes a protein (DmInsP(3)R) that is approximately 60% conserved with mammalian InsP(3)Rs. A number of itpr...

journal_title：Biophysical journal

authors： Srikanth S,Wang Z,Tu H,Nair S,Mathew MK,Hasan G,Bezprozvanny I

更新日期：2004-06-01 00:00:00

abstract：:Single-molecule epifluorescence microscopy was used to observe the translational motion of GPI-linked and native I-E(k) class II MHC membrane proteins in the plasma membrane of CHO cells. The purpose of the study was to look for deviations from Brownian diffusion that might arise from barriers to this motion. Detergen...

journal_title：Biophysical journal

authors： Vrljic M,Nishimura SY,Brasselet S,Moerner WE,McConnell HM

更新日期：2002-11-01 00:00:00

abstract：:Understanding the mechanism of saturated fatty acid-induced hepatocyte toxicity may provide insight into cures for diseases such as obesity-associated cirrhosis. Trehalose, a nonreducing disaccharide shown to protect proteins and cellular membranes from inactivation or denaturation caused by different stress condition...

journal_title：Biophysical journal

authors： Leekumjorn S,Wu Y,Sum AK,Chan C

更新日期：2008-04-01 00:00:00

abstract：:All-atom molecular dynamics simulations of the ion current through OmpF, the major porin in the outer membrane of Escherichia coli, were performed. Starting from the crystal structure, the all-atom modeling allows us to calculate a parameter-free ion conductance in semiquantitative agreement with experiment. Discrepan...

journal_title：Biophysical journal

authors： Pezeshki S,Chimerel C,Bessonov AN,Winterhalter M,Kleinekathöfer U

更新日期：2009-10-07 00:00:00