当前位置： SCI文献检索 > BIOPHYSICAL JOURNAL期刊下所有文献 > The properties of chondrocyte membrane reservoirs and their role in impact-induced cell death.

The properties of chondrocyte membrane reservoirs and their role in impact-induced cell death.

Abstract：

:Impact loading of articular cartilage causes extensive chondrocyte death. Cell membranes have a limited elastic range of 3-4% strain but are protected from direct stretch during physiological loading by their membrane reservoir, an intricate pattern of membrane folds. Using a finite-element model, we suggested previously that access to the membrane reservoir is strain-rate-dependent and that during impact loading, the accessible membrane reservoir is drastically decreased, so that strains applied to chondrocytes are directly transferred to cell membranes, which fail when strains exceed 3-4%. However, experimental support for this proposal is lacking. The purpose of this study was to measure the accessible membrane reservoir size for different membrane strain rates using membrane tethering techniques with atomic force microscopy. We conducted atomic force spectroscopy on isolated chondrocytes (n = 87). A micron-sized cantilever was used to extract membrane tethers from cell surfaces at constant pulling rates. Membrane tethers could be identified as force plateaus in the resulting force-displacement curves. Six pulling rates were tested (1, 5, 10, 20, 40, and 80 μm/s). The size of the membrane reservoir, represented by the membrane tether surface areas, decreased exponentially with increasing pulling rates. The current results support our theoretical findings that chondrocytes exposed to impact loading die because of membrane ruptures caused by high tensile membrane strain rates.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Moo EK,Amrein M,Epstein M,Duvall M,Abu Osman NA,Pingguan-Murphy B,Herzog W

doi

10.1016/j.bpj.2013.08.035

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

1590-600

issue

7

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(13)00982-X

journal_volume

105

pub_type

杂志文章

相关文献

BIOPHYSICAL JOURNAL文献大全
  • Early events in cell spreading as a model for quantitative analysis of biomechanical events.

    abstract：:In this review, we focus on the early events in the process of fibroblast spreading on fibronectin matrices of different rigidities. We present a focused position piece that illustrates the many different tests that a cell makes of its environment before it establishes mature matrix adhesions. When a fibroblast is pla...

    journal_title：Biophysical journal

    pub_type： 杂志文章,评审

    doi：10.1016/j.bpj.2014.10.041

    authors： Wolfenson H,Iskratsch T,Sheetz MP

    更新日期：2014-12-02 00:00:00

  • Models of beta-amyloid ion channels in the membrane suggest that channel formation in the bilayer is a dynamic process.

    abstract：:Here we model the Alzheimer beta-peptide ion channel with the goal of obtaining insight into the mechanism of amyloid toxicity. The models are built based on NMR data of the oligomers, with the universal U-shaped (strand-turn-strand) motif. After 30-ns simulations in the bilayer, the channel dimensions, shapes and sub...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.107.110148

    authors： Jang H,Zheng J,Nussinov R

    更新日期：2007-09-15 00:00:00

  • Single vesicle assaying of SNARE-synaptotagmin-driven fusion reveals fast and slow modes of both docking and fusion and intrasample heterogeneity.

    abstract：:Lipid mixing between vesicles functionalized with SNAREs and the cytosolic C2AB domain of synaptotagmin-1 recapitulates the basic Ca(2+) dependence of neuronal exocytosis. However, in the conventional ensemble lipid mixing assays it is not possible to discriminate whether Ca(2+) accelerates the docking or the fusion o...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2010.12.3730

    authors： Christensen SM,Mortensen MW,Stamou DG

    更新日期：2011-02-16 00:00:00

  • Change in membrane elastic modulus on activation of glucose transport system of brush border membrane vesicles studied by osmotic swelling and dynamic light scattering.

    abstract：:The cell membrane having a transport system is inferred to be flexible when its function is being activated. For the brush border membrane vesicles prepared from rat small intestine, which have the co-transport system of Na+ and glucose, the membrane elasticity was measured as a function of the d-glucose concentration...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(88)83130-8

    authors： Miyamoto S,Maeda T,Fujime S

    更新日期：1988-04-01 00:00:00

  • An anionic phospholipid enables the hydrophobic surfactant proteins to alter spontaneous curvature.

    abstract：:The hydrophobic surfactant proteins, SP-B and SP-C, greatly accelerate the adsorption of the surfactant lipids to an air/water interface. Previous studies of factors that affect curvature suggest that vesicles may adsorb via a rate-limiting structure with prominent negative curvature, in which the hydrophilic face of ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2012.12.041

    authors： Chavarha M,Loney RW,Rananavare SB,Hall SB

    更新日期：2013-02-05 00:00:00

  • From Gene to Function: Cell-Free Electrophysiological and Optical Analysis of Ion Pumps in Nanodiscs.

    abstract：:Nanodiscs that hold a lipid bilayer surrounded by a boundary of scaffold proteins have emerged as a powerful tool for membrane protein solubilization and analysis. By combining nanodiscs and cell-free expression technologies, even completely detergent-free membrane protein characterization protocols can be designed. N...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2017.03.026

    authors： Henrich E,Sörmann J,Eberhardt P,Peetz O,Mezhyrova J,Morgner N,Fendler K,Dötsch V,Wachtveitl J,Bernhard F,Bamann C

    更新日期：2017-09-19 00:00:00

  • Effects of chain unsaturation on the equation of state for lipid monolayers at the air-water interface.

    abstract：:An equation of state for lipid monolayers at the air-water interface is presented, which takes into account the effects of the conformation and the number and position of double bonds of the hydrocarbon chains. The total Hamiltonian of the monolayer is assumed to consist of three terms. Two of them are calculated exac...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(95)79919-2

    authors： Feng SS,MacDonald RC

    更新日期：1995-08-01 00:00:00

  • Structural relaxation and nonexponential kinetics of CO-binding to horse myoglobin. Multiple flash photolysis experiments.

    abstract：:The geminate recombination kinetics of CO-myoglobin strongly deviates from single exponential behavior in contrast to what is expected for unimolecular reactions (1). At low temperatures, this result was attributed to slowly exchanging conformational states which differ substantially in barrier height for ligand bindi...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(93)81554-6

    authors： Post F,Doster W,Karvounis G,Settles M

    更新日期：1993-06-01 00:00:00

  • Bacterial Flagellar Motor Switch in Response to CheY-P Regulation and Motor Structural Alterations.

    abstract：:The bacterial flagellar motor (BFM) is a molecular machine that rotates the helical filaments and propels the bacteria swimming toward favorable conditions. In our previous works, we built a stochastic conformational spread model to explain the dynamic and cooperative behavior of BFM switching. Here, we extended this ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2016.02.023

    authors： Ma Q,Sowa Y,Baker MA,Bai F

    更新日期：2016-03-29 00:00:00

  • Evidence for phospholipid microdomain formation in liquid crystalline liposomes reconstituted with Escherichia coli lactose permease.

    abstract：:The well-characterized integral membrane protein lactose (lac) permease from Escherichia coli was reconstituted together with trace amounts (molar fraction X = 0.005 of the total phospholipid) of different pyrene-labeled phospholipid analogs into 1-palmitoyl-2-oleoyl-sn-glycero-3-sn-glycero-3-phospho-rac'-glycerol (PO...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(97)78771-X

    authors： Lehtonen JY,Kinnunen PK

    更新日期：1997-03-01 00:00:00

  • Protein and virus crystal growth on international microgravity laboratory-2.

    abstract：:Two T = 1 and one T = 3 plant viruses, along with a protein, were crystallized in microgravity during the International Microgravity Laboratory-2 (IML-2) mission in July of 1994. The method used was liquid-liquid diffusion in the European Space Agency's Advanced Protein Crystallization Facility (APCF). Distinctive alt...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(95)79890-3

    authors： Koszelak S,Day J,Leja C,Cudney R,McPherson A

    更新日期：1995-07-01 00:00:00

  • Protein-induced surface structuring in myelin membrane monolayers.

    abstract：:Monolayers prepared from myelin conserve all the compositional complexity of the natural membrane when spread at the air-water interface. They show a complex pressure-dependent surface pattern that, on compression, changes from the coexistence of two liquid phases to a viscous fractal phase embedded in a liquid phase....

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.107.112441

    authors： Rosetti CM,Maggio B

    更新日期：2007-12-15 00:00:00

  • Molecular dynamics simulation of a dipalmitoylphosphatidylcholine bilayer with NaCl.

    abstract：:Molecular dynamics simulations are performed on two hydrated dipalmitoylphosphatidylcholine bilayer systems: one with pure water and one with added NaCl. Due to the rugged nature of the membrane/electrolyte interface, ion binding to the membrane surface is characterized by the loss of ion hydration. Using this structu...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(03)75102-9

    authors： Pandit SA,Bostick D,Berkowitz ML

    更新日期：2003-06-01 00:00:00

  • Origins of individual swimming behavior in bacteria.

    abstract：:Cells in a cloned population of coliform bacteria exhibit a wide range of swimming behaviors--a form of non-genetic individuality. We used computer models to examine the proposition that these variations are due to differences in the number of chemotaxis signaling molecules from one cell to the next. Simulations were ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(98)77777-X

    authors： Levin MD,Morton-Firth CJ,Abouhamad WN,Bourret RB,Bray D

    更新日期：1998-01-01 00:00:00

  • The Josephin domain determines the morphological and mechanical properties of ataxin-3 fibrils.

    abstract：:Fibrillar aggregation of the protein ataxin-3 is linked to the inherited neurodegenerative disorder Spinocerebellar ataxia type 3, a member of the polyQ expansion disease family. We previously reported that aggregation and stability of the nonpathological form of ataxin-3, carrying an unexpanded polyQ tract, are modul...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2011.02.056

    authors： Masino L,Nicastro G,De Simone A,Calder L,Molloy J,Pastore A

    更新日期：2011-04-20 00:00:00

  • The origin of nonmonotonic complex behavior and the effects of nonnative interactions on the diffusive properties of protein folding.

    abstract：:We present a method for calculating the configurational-dependent diffusion coefficient of a globular protein as a function of the global folding process. Using a coarse-grained structure-based model, we determined the diffusion coefficient, in reaction coordinate space, as a function of the fraction of native contact...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2010.04.041

    authors： Oliveira RJ,Whitford PC,Chahine J,Wang J,Onuchic JN,Leite VB

    更新日期：2010-07-21 00:00:00

  • Free energies of molecular bound states in lipid bilayers: lethal concentrations of antimicrobial peptides.

    abstract：:The lipid matrix, or the lipid bilayer, of cell membranes is a natural binding site for amphipathic molecules, including antimicrobial peptides, pore-forming proteins, and many drugs. The unique property of pore-forming antimicrobial peptides is that they exhibit a threshold concentration (called the lethal concentrat...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2009.01.030

    authors： Huang HW

    更新日期：2009-04-22 00:00:00

  • Time-resolved FTIR studies of sensory rhodopsin II (NpSRII) from Natronobacterium pharaonis: implications for proton transport and receptor activation.

    abstract：:The photocycle of the photophobic receptor from Natronobacterium pharaonis, NpSRII, is studied by static and time-resolved step-scan Fourier transform infrared spectroscopy. Both low-temperature static and time-resolved spectra resolve a K-like intermediate, and the corresponding spectra show little difference within ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(03)74935-2

    authors： Hein M,Wegener AA,Engelhard M,Siebert F

    更新日期：2003-02-01 00:00:00

  • Smoothed acyl chain orientational order parameter profiles in dimyristoylphosphatidylcholine-distearoylphosphatidylcholine mixtures: a 2H-NMR study.

    abstract：:The accommodation of chain-length mismatch in liquid crystal phase bilayers was examined by using deuterium nuclear magnetic resonance to obtain smoothed orientational order parameter profiles for acyl chains of both components in binary lipid mixture bilayers. Mixtures of dimyristoylphosphatidylcholine (DMPC) and dis...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(95)80219-5

    authors： Lu D,Vavasour I,Morrow MR

    更新日期：1995-02-01 00:00:00

  • Modeling of glucose-induced cAMP oscillations in pancreatic β cells: cAMP rocks when metabolism rolls.

    abstract：:Recent advances in imaging technology have revealed oscillations of cyclic adenosine monophosphate (cAMP) in insulin-secreting cells. These oscillations may be in phase with cytosolic calcium oscillations or out of phase. cAMP oscillations have previously been modeled as driven by oscillations in calcium, based on the...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2015.06.024

    authors： Peercy BE,Sherman AS,Bertram R

    更新日期：2015-07-21 00:00:00

  • Salt dependence of the elasticity and overstretching transition of single DNA molecules.

    abstract：:As double-stranded DNA is stretched to its B-form contour length, models of polymer elasticity can describe the dramatic increase in measured force. When the molecule is stretched beyond this contour length, it shows a highly cooperative overstretching transition. We have measured the elasticity and overstretching tra...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(02)75658-0

    authors： Wenner JR,Williams MC,Rouzina I,Bloomfield VA

    更新日期：2002-06-01 00:00:00

  • Effects of mRNA Degradation and Site-Specific Transcriptional Pausing on Protein Expression Noise.

    abstract：:Genetically identical cells exhibit diverse phenotypes even when experiencing the same environment. This phenomenon in part originates from cell-to-cell variability (noise) in protein expression. Although various kinetic schemes of stochastic transcription initiation are known to affect gene expression noise, how post...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2018.02.010

    authors： Kim S,Jacobs-Wagner C

    更新日期：2018-04-10 00:00:00

  • Single-molecule enzymology of chymotrypsin using water-in-oil emulsion.

    abstract：:Single-molecule studies allow the study of subtle activity differences due to local folding in proteins, but are time consuming and difficult because only a few molecules are observed in one experiment. We developed an assay where we can simultaneously measure the activity of hundreds of individual molecules. The assa...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1529/biophysj.104.055053

    authors： Lee AI,Brody JP

    更新日期：2005-06-01 00:00:00

  • Interfacial polar interactions affect gramicidin channel kinetics.

    abstract：:Critical to biological processes such as secretion and transport, protein-lipid interactions within the membrane and at the membrane-water interface still raise many questions. Here we examine the role of lipid headgroups in these interactions by using gramicidin A (gA) channels in planar bilayers as a probe. We show ...

    journal_title：Biophysical journal

    pub_type： 信件

    doi：10.1529/biophysj.107.120261

    authors： Rostovtseva TK,Petrache HI,Kazemi N,Hassanzadeh E,Bezrukov SM

    更新日期：2008-02-15 00:00:00

  • Vibrational fluctuations of hydrogen bonds in a DNA double helix with nonuniform base pairs.

    abstract：:The Green's function technique is applied to a study of breathing modes in a DNA double helix which contains a region of different base pairs from the rest of the double helix. The calculation is performed on a G-C helix in the B conformation with four consecutive base pairs replaced by A-T. The average stretch in hyd...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(90)82570-4

    authors： Feng Y,Prohofsky EW

    更新日期：1990-03-01 00:00:00

  • Permeability, phase-boundary potential and conductance in a cholinergic channel without constant field.

    abstract：:A potassium-selective, chemically excitable channel, whose characteristics cannot be accurately described by constant-field theory, is studied by a new approach based on diffusion theory but with no need for the classical assumptions of constant field, homogeneous membrane, and equal phase-boundary potentials at both ...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(77)85617-8

    authors： Schwartz TL,Kado RT

    更新日期：1977-06-01 00:00:00

  • The Interplay between Molten Globules and Heme Disassociation Defines Human Hemoglobin Disassembly.

    abstract：:Hemoglobin functions as a tetrameric oxygen transport protein, with each subunit containing a heme cofactor. Its denaturation, either in vivo or in vitro, involves autoxidation to methemoglobin, followed by cofactor loss and globin unfolding. We have proposed a global disassembly scheme for human methemoglobin, linkin...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2020.01.031

    authors： Samuel PP,White MA,Ou WC,Case DA,Phillips GN Jr,Olson JS

    更新日期：2020-03-24 00:00:00

  • Dynamic imaging of lateral diffusion by electron spin resonance and study of rotational dynamics in model membranes. Effect of cholesterol.

    abstract：:The effects of cholesterol on the dynamics and the structural properties of two different spin probes, the sterol type CSL and the phospholipid type 16-PC, in POPC/cholesterol oriented multilayer model membranes were examined. Our results are consistent with a nonideal solution containing cholesterol-rich clusters cre...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(89)82847-4

    authors： Shin YK,Freed JH

    更新日期：1989-03-01 00:00:00

  • Differences in β-strand populations of monomeric Aβ40 and Aβ42.

    abstract：:Using homonuclear (1)H NOESY spectra, with chemical shifts, (3)JH(N)H(α) scalar couplings, residual dipolar couplings, and (1)H-(15)N NOEs, we have optimized and validated the conformational ensembles of the amyloid-β 1-40 (Aβ40) and amyloid-β 1-42 (Aβ42) peptides generated by molecular dynamics simulations. We find t...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/j.bpj.2013.04.056

    authors： Ball KA,Phillips AH,Wemmer DE,Head-Gordon T

    更新日期：2013-06-18 00:00:00

  • A new method to study shape recovery of red blood cells using multiple optical trapping.

    abstract：:In this new method for studying the shape recovery of deformed red blood cells, three optical traps ("optical tweezers") induce a parachute-shaped red cell deformation, which is comparable to the deformation in small capillaries. The shape recovery is recorded, and a relaxation time is obtained for each individual red...

    journal_title：Biophysical journal

    pub_type： 杂志文章

    doi：10.1016/S0006-3495(95)80084-6

    authors： Bronkhorst PJ,Streekstra GJ,Grimbergen J,Nijhof EJ,Sixma JJ,Brakenhoff GJ

    更新日期：1995-11-01 00:00:00