Abstract:
:In this new method for studying the shape recovery of deformed red blood cells, three optical traps ("optical tweezers") induce a parachute-shaped red cell deformation, which is comparable to the deformation in small capillaries. The shape recovery is recorded, and a relaxation time is obtained for each individual red blood cell. The sensitivity of this technique for the detection of differences in relaxation times is demonstrated on subpopulations of density-separated red blood cells: "young" cells have shorter (162 ms) and "old" cells have longer (353 ms) relaxation times compared with the total population (271 ms). The relaxation time is remarkably shorter (114 ms) when the plasma surrounding the cells is replaced by a phosphate-buffered saline solution. The main advantages of this technique are the relatively short measuring and preparation time and the physiological type of deformation and shape recovery in which all relevant cell properties play a role. Therefore, especially when automated further, the technique may be a powerful tool for the study of (sub)populations of pathological red blood cells.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Bronkhorst PJ,Streekstra GJ,Grimbergen J,Nijhof EJ,Sixma JJ,Brakenhoff GJdoi
10.1016/S0006-3495(95)80084-6subject
Has Abstractpub_date
1995-11-01 00:00:00pages
1666-73issue
5eissn
0006-3495issn
1542-0086pii
S0006-3495(95)80084-6journal_volume
69pub_type
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