DNA release from lipoplexes by anionic lipids: correlation with lipid mesomorphism, interfacial curvature, and membrane fusion.

abstract：:Isothermal (27 degrees C) phase behavior of dimyristoyl phosphatidyl choline-cholesterol mixtures at various osmotic pressures and cholesterol contents was investigated by means of isothermal sorption microcalorimetry and (2)H-nuclear magnetic resonance. The calorimetric method allows for simultaneous measurement of t...

journal_title：Biophysical journal

authors： Sparr E,Hallin L,Markova N,Wennerström H

更新日期：2002-10-01 00:00:00

abstract：:Cholesterol, due to its condensing effect, is considered an important regulator of membrane thickness. Other sterols, due to their structural similarities to cholesterol, are often assumed to have a universal effect on membrane properties similar to the condensing effect of cholesterol, albeit possibly to different de...

journal_title：Biophysical journal

authors： Hung WC,Lee MT,Chung H,Sun YT,Chen H,Charron NE,Huang HW

更新日期：2016-05-10 00:00:00

abstract：:Molecular dynamics simulations have been applied to the DNA octamer d(GCGCA-GAAC). d(GTTCGCGC), which has an adenine bulge at the center to determine the pathway for interconversion between the stacked and extended forms. These forms are known to be important in the molecular recognition of bulges. From a total of ~35...

journal_title：Biophysical journal

authors： Feig M,Zacharias M,Pettitt BM

更新日期：2001-07-01 00:00:00

abstract：:The voltage dependence of light-induced proton pumping was studied with bacteriorhodopsin (bR) from Halobacterium salinarum, expressed in the plasma membrane of oocytes from Xenopus laevis in the range -160 mV to +60 mV at different light intensities. Depending on the applied field, the quenching effect by blue light,...

journal_title：Biophysical journal

authors： Nagel G,Kelety B,Möckel B,Büldt G,Bamberg E

更新日期：1998-01-01 00:00:00

abstract：:Alamethicin is a 19-residue hydrophobic peptide, which is extended by a C-terminal phenylalaninol but lacks residues that might anchor the ends of the peptide at the lipid-water interface. Voltage-dependent ion channels formed by alamethicin depend strongly in their characteristics on chain length of the host lipid me...

journal_title：Biophysical journal

authors： Marsh D,Jost M,Peggion C,Toniolo C

更新日期：2007-06-01 00:00:00

abstract：:Na(+) conductance through cloned K(+) channels has previously allowed characterization of inactivation and K(+) binding within the pore, and here we have used Na(+) permeation to study recovery from C-type inactivation in human Kv1.5 channels. Replacing K(+) in the solutions with Na(+) allows complete Kv1.5 inactivati...

journal_title：Biophysical journal

authors： Wang Z,Hesketh JC,Fedida D

更新日期：2000-11-01 00:00:00

abstract：:The cytoplasmic surface of the BR (initial) state of bacteriorhodopsin is characterized by a cluster of three carboxylates that function as a proton-collecting antenna. Systematic replacement of most of the surface carboxylates indicated that the cluster is made of D104, E161, and E234 (Checover, S., Y. Marantz, E. Na...

journal_title：Biophysical journal

authors： Nachliel E,Gutman M,Tittor J,Oesterhelt D

更新日期：2002-07-01 00:00:00

abstract：:The stability of the structure of double-stranded DNA in the salt-free solution is discussed on the basis of the polyelectrolyte theory. Assuming that DNA is an infinitely long rod, and the formation of double strands is divided into combining process and folding process, the free energy changes required in these proc...

journal_title：Biophysical journal

authors： OHNISHI T

更新日期：1963-11-01 00:00:00

abstract：:It has been a significant challenge to quantitatively study the dynamic intracellular processes in live cells. These studies are essential for a thorough understanding of the underlying mechanisms regulating the signaling pathways and the transitions between cell cycle stages. Our studies of Cdc20, an important mitoti...

journal_title：Biophysical journal

authors： Wang Z,Shah JV,Berns MW,Cleveland DW

更新日期：2006-07-01 00:00:00

abstract：:Phenotypic plasticity is posed to be a vital trait of cancer cells such as circulating tumor cells, allowing them to undergo reversible or irreversible switching between phenotypic states important for tumorigenesis and metastasis. While irreversible phenotypic switching can be detected by studying the genome, reversi...

journal_title：Biophysical journal

authors： Aw Yong KM,Sun Y,Merajver SD,Fu J

更新日期：2017-03-28 00:00:00

abstract：:High-bandwidth measurements of the ion current through hafnium oxide and silicon nitride nanopores allow the analysis of sub-30 kD protein molecules with unprecedented time resolution and detection efficiency. Measured capture rates suggest that at moderate transmembrane bias values, a substantial fraction of protein ...

journal_title：Biophysical journal

authors： Larkin J,Henley RY,Muthukumar M,Rosenstein JK,Wanunu M

更新日期：2014-02-04 00:00:00

abstract：:Some membrane peptides, such as Alamethicin, form barrel-stave aggregates with a broad probability distribution of size (number of peptides in the aggregate). This distribution has been shown to depend on the characteristics of the lipid bilayer. A mechanism for this influence is suggested, in analogy to earlier work ...

journal_title：Biophysical journal

authors： Cantor RS

更新日期：2002-05-01 00:00:00

abstract：:The polymorphic phase behavior of bovine heart cardiolipin (CL) in the presence of different divalent cations and the kinetics of CL vesicle fusion induced by these cations have been investigated. (31)P-NMR measurements of equilibrium cation-CL complexes showed the lamellar-to-hexagonal (L(alpha)-H(II)) transition tem...

journal_title：Biophysical journal

authors： Ortiz A,Killian JA,Verkleij AJ,Wilschut J

更新日期：1999-10-01 00:00:00

abstract：:Using a model for catalysis of a dynamic equilibrium, the role of constraint in catalysis is quantified. The intrinsic rigidity of proteins is shown to be insufficient to constrain the activated complexes of enzymes, irrespective of the mechanism. However, when minimization of the surface excess free energy of water s...

journal_title：Biophysical journal

authors： Vanselow DG

更新日期：2002-05-01 00:00:00

abstract：:Muscles have evolved to power a wide variety of movements. A protein component critical to varying power generation is the myosin isoform present in the muscle. However, how functional variation in muscle arises from myosin structure is not well understood. We studied the influence of the converter, a myosin structura...

journal_title：Biophysical journal

authors： Glasheen BM,Ramanath S,Patel M,Sheppard D,Puthawala JT,Riley LA,Swank DM

更新日期：2018-03-13 00:00:00

abstract：:Recent measurements have demonstrated enzyme activity at hydrations as low as 3%. This raises the question of whether hydration-induced enzyme flexibility is important for activity. Here, to address this, picosecond dynamic neutron scattering experiments are performed on pig liver esterase powders at 0%, 3%, 12%, and ...

journal_title：Biophysical journal

authors： Kurkal V,Daniel RM,Finney JL,Tehei M,Dunn RV,Smith JC

更新日期：2005-08-01 00:00:00

abstract：:Membrane potential of aortic endothelial cells under resting conditions is dominated by inward-rectifier K(+) channels belonging to the Kir 2 family. Regulation of endothelial Kir by membrane cholesterol was studied in bovine aortic endothelial cells by altering the sterol composition of the cell membrane. Our results...

journal_title：Biophysical journal

authors： Romanenko VG,Rothblat GH,Levitan I

更新日期：2002-12-01 00:00:00

abstract：:Dendrites of cerebellar Purkinje cells (PCs) respond to brief excitations from parallel fibers with lasting plateau depolarizations. It is unknown whether these plateaus are local events that boost the synaptic signals or they propagate to the soma and directly take part in setting the cell firing dynamics. To address...

journal_title：Biophysical journal

authors： Genet S,Sabarly L,Guigon E,Berry H,Delord B

更新日期：2010-07-21 00:00:00

abstract：:We present direct quasielastic neutron scattering measurements, in vivo, of macromolecular dynamics in Escherichia coli. The experiments were performed on a wide range of timescales to cover the large panel of internal and self-diffusion motions. Three major internal processes were extracted at physiological temperatu...

journal_title：Biophysical journal

authors： Jasnin M,Moulin M,Haertlein M,Zaccai G,Tehei M

更新日期：2008-07-01 00:00:00

abstract：:Emission spectra of the photolabile Ca2+ chelators DM-nitrophen, nitr-5, and diazo-2 were studied alone, and in the presence of indo-1, to investigate potential interactions that would make the simultaneous manipulation and ratiometric measurement of the intracellular Ca2+ concentration difficult. Neither diazo-2 nor ...

journal_title：Biophysical journal

authors： Hadley RW,Kirby MS,Lederer WJ,Kao JP

更新日期：1993-12-01 00:00:00

abstract：:Five molecular dynamics computer simulations were performed on different phospholipid:sterol membrane systems in order to study the influence of sterol structure on membrane properties. Three of these simulated bilayer systems were composed of a 1:8 sterol:phospholipid ratio, each of which employed one of the sterol m...

journal_title：Biophysical journal

authors： Smondyrev AM,Berkowitz ML

更新日期：2001-04-01 00:00:00

abstract：:The interaction between protein molecules and the hydroxyapatite (HAP) crystal is an important research topic in many fields. However, the nature of their noncovalent bonding is still not clear at the atomic level. In this work, molecular dynamics simulation, steered molecular dynamics simulation, and quantum chemistr...

journal_title：Biophysical journal

authors： Dong X,Wang Q,Wu T,Pan H

更新日期：2007-08-01 00:00:00

abstract：:Determining the spatial relationship of individual proteins in dense assemblies remains a challenge for superresolution nanoscopy. The organization of aquaporin-4 (AQP4) into large plasma membrane assemblies provides an opportunity to image membrane-bound AQP4 antibodies (AQP4-IgG) and evaluate changes in their spatia...

journal_title：Biophysical journal

authors： Soltys JN,Meyer SA,Schumann H,Gibson EA,Restrepo D,Bennett JL

更新日期：2017-04-25 00:00:00

abstract：:The structure and dynamics of a large segment of Ste2p, the G-protein-coupled alpha-factor receptor from yeast, were studied in dodecylphosphocholine (DPC) micelles using solution NMR spectroscopy. We investigated the 73-residue peptide EL3-TM7-CT40 consisting of the third extracellular loop 3 (EL3), the seventh trans...

journal_title：Biophysical journal

authors： Neumoin A,Arshava B,Becker J,Zerbe O,Naider F

更新日期：2007-07-15 00:00:00

abstract：:The effect of intracellular (i) and extracellular (o) Na+ on pre-steady-state transient current associated with Na+/Na+ exchange by the Na+/K+ pump was investigated in the vegetal pole of Xenopus oocytes. Current records in response to 40-ms voltage pulses from -180 to +100 mV in the absence of external Na+ were subtr...

journal_title：Biophysical journal

authors： Holmgren M,Rakowski RF

更新日期：2006-03-01 00:00:00

abstract：:The arrangement of side chains in alpha-helical regions of protein can be represented by helical net-diagrams. The addition of quantitative indices of the character of the residue at each net-point facilitates a straightforward examination of the groupings of residues of different character. As such the method complem...

journal_title：Biophysical journal

authors： Dunnill P

更新日期：1968-07-01 00:00:00

abstract：:The structural properties of a crucial transmembrane helix for proton translocation in vacuolar ATPase are studied using double site-directed spin-labeling combined with electron spin resonance (ESR) (or electron paramagnetic resonance) and circular dichroism spectroscopy in sodium dodecyl sulfate micelles. For this p...

journal_title：Biophysical journal

authors： Vos WL,Vermeer LS,Hemminga MA

更新日期：2007-01-01 00:00:00

abstract：:Sphingomyelin is an abundant lipid in some cellular membrane domains, such as lipid rafts. Hydrogen bonding and hydrophobic interactions of the lipid with surrounding components such as neighboring sphingomyelin and cholesterol (Cho) are widely considered to stabilize the raft-like liquid-ordered (Lo) domains in membr...

journal_title：Biophysical journal

authors： Yano Y,Hanashima S,Yasuda T,Tsuchikawa H,Matsumori N,Kinoshita M,Al Sazzad MA,Slotte JP,Murata M

更新日期：2018-10-16 00:00:00

abstract：:A new microscopic technique is demonstrated that combines attributes from both near-field scanning optical microscopy (NSOM) and fluorescence resonance energy transfer (FRET). The method relies on attaching the acceptor dye of a FRET pair to the end of a near-field fiber optic probe. Light exiting the NSOM probe, whic...

journal_title：Biophysical journal

authors： Vickery SA,Dunn RC

更新日期：1999-04-01 00:00:00

abstract：:Cationic amino acid transporters are highly selective for L-enantiomers such as L-arginine (L-Arg). Because of this stereoselectivity, little is known about the interaction of these transporters with D-isomers. To study whether these compounds can provide information on the molecular mechanism of transport, inward cur...

journal_title：Biophysical journal

authors： Zhou J,Peluffo RD

更新日期：2010-11-17 00:00:00