Does the use of DM-nitrophen, nitr-5, or diazo-2 interfere with the measurement of indo-1 fluorescence?

abstract：:Molecular dynamics simulations are presented for a Thermus aquaticus (Taq) DNA polymerase I complex (consisting of the protein, the primer-template DNA strands, and the incoming nucleotide) subjected to external forces. The results obtained with a force applied to the DNA template strand provide insights into the effe...

journal_title：Biophysical journal

authors： Andricioaei I,Goel A,Herschbach D,Karplus M

更新日期：2004-09-01 00:00:00

abstract：:Deuterium wide line NMR spectroscopy was used to study cholesterol effects on the ceramide portions of two glycosphingolipids (GSLs) distributed as minor components in fluid membranes. The common existence of very long fatty acids on GSLs was taken into account by including one glycolipid species with fatty acid chain...

journal_title：Biophysical journal

authors： Morrow MR,Singh D,Lu D,Grant CW

更新日期：1995-01-01 00:00:00

abstract：:A block normal mode (BNM) algorithm, originally proposed by Tama et al., (Proteins Struct. Func. Genet. 41:1-7, 2000) was implemented into the simulation program CHARMM. The BNM approach projects the hessian matrix into local translation/rotation basis vectors and, therefore, dramatically reduces the size of the matri...

journal_title：Biophysical journal

authors： Li G,Cui Q

更新日期：2002-11-01 00:00:00

abstract：:In ubiquitination, cullin-RING E3 ubiquitin ligases (CRLs) assist in ubiquitin transfer from ubiquitin-conjugating enzyme E2 to the substrate. Neddylation, which involves NEDD8 transfer from E2 to E3-cullin, stimulates ubiquitination by inducing conformational change in CRLs. However, deneddylation, which removes NEDD...

journal_title：Biophysical journal

authors： Liu J,Nussinov R

更新日期：2010-08-04 00:00:00

abstract：:RNA junctions are common secondary structural elements present in a wide range of RNA species. They play crucial roles in directing the overall folding of RNA molecules as well as in a variety of biological functions. In particular, there has been great interest in the dynamics of RNA junctions, including conformation...

journal_title：Biophysical journal

authors： Jung S,Schlick T

更新日期：2014-01-21 00:00:00

abstract：:Unraveling the conformation of membrane-bound viral fusion peptides is essential for understanding how those peptides destabilize the bilayer topology of lipids that is important for virus-cell membrane fusion. Here, molecular dynamics (MD) simulations were performed to investigate the conformation of the 20 amino aci...

journal_title：Biophysical journal

authors： Huang Q,Chen CL,Herrmann A

更新日期：2004-07-01 00:00:00

abstract：:Crossover motifs are integral components for designing DNA-based nanostructures and nanomechanical devices due to their enhanced rigidity compared to the normal B-DNA. Although the structural rigidity of the double helix B-DNA has been investigated extensively using both experimental and theoretical tools, to date the...

journal_title：Biophysical journal

authors： Santosh M,Maiti PK

更新日期：2011-09-21 00:00:00

abstract：:Single-molecule tracking of membrane proteins has become an important tool for investigating dynamic processes in live cells, such as cell signaling, membrane compartmentation or trafficking. The extraction of relevant parameters, such as interaction times between molecular partners or confinement-zone sizes, from the...

journal_title：Biophysical journal

authors： Bouzigues C,Dahan M

更新日期：2007-01-15 00:00:00

abstract：:The elastic rigidity (stiffness) of impaled motionless bull sperm flagella has been determined by a manipulatory technique which permitted direct analytical treatment of the experimental system. The effects of external ATP and ADP were measured. It was found that ATP acts as a plasticizing agent, while ADP does not. T...

journal_title：Biophysical journal

authors： Lindemann CB,Rudd WG,Rikmenspoel R

更新日期：1973-05-01 00:00:00

abstract：:Many aspects of chromatin biology are influenced by the nuclear compartment in which a locus resides, from transcriptional regulation to DNA repair. Further, the dynamic and variable localization of a particular locus across cell populations and over time makes analysis of a large number of cells critical. As a conseq...

journal_title：Biophysical journal

authors： Zhao Y,Schreiner SM,Koo PK,Colombi P,King MC,Mochrie SG

更新日期：2016-07-12 00:00:00

abstract：:The presence of two liquid-crystalline phases, alpha and beta, in mixed bilayers of dimyristoylphosphatidylcholine/cholesterol was detected by the changes in the distribution of the fluorescence lifetimes of t-PnA, as analyzed by the Maximum Entropy Method. The formation of the liquid-ordered beta-phase, in the 30-40 ...

journal_title：Biophysical journal

authors： Reyes Mateo C,Ulises Acuña A,Brochon JC

更新日期：1995-03-01 00:00:00

abstract：:The propagation of sounds and pulse waves within the cardiovascular system is subject to strong dissipative mechanisms. To investigate the effects of blood viscosity on dissipation as well as dispersion of small waves in arteries and veins, a parametric study has been carried out. A linearized analysis of axisymmetric...

journal_title：Biophysical journal

authors： Jones E,Anliker M,Chang ID

更新日期：1971-12-01 00:00:00

abstract：:The effects of the anionic tungsten carbonyl complex [W(CO)(5)SC(6)H(5)](-) and its fluorinated analog [W(CO)(5)SC(6)F(5)](-) on the electrical properties of the plasma membrane of mouse myeloma cells were studied by the single-cell electrorotation technique. At micromolar concentrations, both compounds gave rise to a...

journal_title：Biophysical journal

authors： Kürschner M,Nielsen K,von Langen JR,Schenk WA,Zimmermann U,Sukhorukov VL

更新日期：2000-09-01 00:00:00

abstract：:We apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ in model membranes and live cells. We show that the 1700 ps lifetime shift between liquid-disordered and liquid-ordered phases offers greater contrast than the 60 nm spectral shift previously reported. Detection of cholesterol-rich ...

journal_title：Biophysical journal

authors： Owen DM,Lanigan PM,Dunsby C,Munro I,Grant D,Neil MA,French PM,Magee AI

更新日期：2006-06-01 00:00:00

abstract：:The time-resolved thermodynamics of the flavin mononucleotide (FMN)-binding LOV1 domain of Chlamydomonas reinhardtii phot (phototropin homolog) was studied by means of laser-induced optoacoustic spectroscopy. In the wild-type protein the early red-shifted intermediate LOV(715) exhibits a small volume contraction, Delt...

journal_title：Biophysical journal

authors： Losi A,Kottke T,Hegemann P

更新日期：2004-02-01 00:00:00

abstract：:The excitation of the squid giant axon that had been perfused intra- and extracellularly with solutions containing a high concentration of glycerol could be observed below 0 degree C. The action potential could be elicited at normal strengths of electrical stimuli. The time-course of the action potential was slowed, w...

journal_title：Biophysical journal

authors： Kukita F,Yamagishi S

更新日期：1981-07-01 00:00:00

abstract：:The arrangement of side chains in alpha-helical regions of protein can be represented by helical net-diagrams. The addition of quantitative indices of the character of the residue at each net-point facilitates a straightforward examination of the groupings of residues of different character. As such the method complem...

journal_title：Biophysical journal

authors： Dunnill P

更新日期：1968-07-01 00:00:00

abstract：:We present a probability density approach to modeling localized Ca2+ influx via L-type Ca2+ channels and Ca2+-induced Ca2+ release mediated by clusters of ryanodine receptors during excitation-contraction coupling in cardiac myocytes. Coupled advection-reaction equations are derived relating the time-dependent probabi...

journal_title：Biophysical journal

authors： Williams GS,Huertas MA,Sobie EA,Jafri MS,Smith GD

更新日期：2007-04-01 00:00:00

abstract：:The impedance measured in a strip of heart tissue from the moth Hyalophora cecropia is fitted by circuit models of several configurations. The circuits include: (a) a single R-C circuit (b) a double R-C circuit (c) terminated transmission lines, and (d) a pattern of cells with cell-to-cell transmission paths. The last...

journal_title：Biophysical journal

authors： Stibitz GR,McCann FV

更新日期：1974-02-01 00:00:00

abstract：:In the photocycle of bacteriorhodopsin at pH 7, a proton is ejected to the extracellular medium during the protonation of Asp-85 upon formation of the M intermediate. The group that releases the ejected proton does not become reprotonated until the prephotolysis state is restored from the N and O intermediates. In con...

journal_title：Biophysical journal

authors： Morgan JE,Vakkasoglu AS,Lanyi JK,Lugtenburg J,Gennis RB,Maeda A

更新日期：2012-08-08 00:00:00

abstract：:We have used anomalous small-angle x-ray scattering as a structural probe for solutions of rabbit parvalbumin labeled with terbium. This technique makes use of the large changes in the terbium scattering factor that occur when the x-ray energy is tuned around an L3 absorption edge of this heavy-atom label. These chang...

journal_title：Biophysical journal

authors： Miake-Lye RC,Doniach S,Hodgson KO

更新日期：1983-03-01 00:00:00

abstract：:The mechanical properties of living cells are a label-free biophysical marker of cell viability and health; however, their use has been greatly limited by low measurement throughput. Although examining individual cells at high rates is now commonplace with fluorescence activated cell sorters, development of comparable...

journal_title：Biophysical journal

authors： Sawetzki T,Eggleton CD,Desai SA,Marr DW

更新日期：2013-11-19 00:00:00

abstract：:Erythrocytes were electrofused with multiple rectangular voltage pulses to show an oscillatory movement, divided into swell phases and pump events. During each swell phase, which lasted from 0.5 s to more than 180 s, the fused cells' (doublets') volume increased by colloid osmotic swelling, and the membrane area was e...

journal_title：Biophysical journal

authors： Baumann M

更新日期：1999-11-01 00:00:00

abstract：:The rate of exchange of urea across the membranes of human erythrocytes (red blood cells) was quantified on the 1-s to 2-min timescale. (13)C-urea was hyperpolarized and subjected to rapid dissolution and the previously reported (partial) resolution of (13)C NMR resonances from the molecules inside and outside red blo...

journal_title：Biophysical journal

authors： Pagès G,Puckeridge M,Liangfeng G,Tan YL,Jacob C,Garland M,Kuchel PW

更新日期：2013-11-05 00:00:00

abstract：:Nonequilibrium molecular dynamics (NEMD) computer simulations are used to calculated the bulk modulus for a dimyristoylphosphatidylcholine bilayer. A methodology is developed whereby NEMD can be effectively used to calculate material properties for complex systems that undergo long time-scale conformational changes. I...

journal_title：Biophysical journal

authors： Ayton G,Smondyrev AM,Bardenhagen SG,McMurtry P,Voth GA

更新日期：2002-03-01 00:00:00

abstract：:Fusion of lipid bilayers proceeds via a sequence of distinct structural transformations. Its early stage involves a localized, hemifused intermediate in which the proximal but not yet the distal monolayers are connected. Whereas the so-called stalk model most successfully accounts for the properties of the hemifused i...

journal_title：Biophysical journal

authors： May S

更新日期：2002-12-01 00:00:00

abstract：:Two modes of behavior of single human red cells in a shear field have been described. It is known that in low viscosity media and at shear rates less than 20 s-1, the cells rotate with a periodically varying angular velocity, in accord with the theory of Jeffery (1922) for oblate spheroids. In media of viscosity great...

journal_title：Biophysical journal

authors： Bitbol M

更新日期：1986-05-01 00:00:00

abstract：:Large bottlebrush complexes formed from the polysaccharide hyaluronan (HA) and the proteoglycan aggrecan contribute to cartilage compression resistance and are necessary for healthy joint function. A variety of mechanical forces act on these complexes in the cartilage extracellular matrix, motivating the need for a qu...

journal_title：Biophysical journal

authors： Innes-Gold SN,Berezney JP,Saleh OA

更新日期：2020-10-06 00:00:00

abstract：:Infrared spectroscopy has long been used to examine the average secondary structure and orientation of membrane proteins. With the recent utilization of site-specific isotope labeling (e.g., peptidic 1-(13)C = (18)O) it is now possible to examine localized properties, rather than global averages. The technique of site...

journal_title：Biophysical journal

authors： Arbely E,Kass I,Arkin IT

更新日期：2003-10-01 00:00:00

abstract：:In this review, we focus on the early events in the process of fibroblast spreading on fibronectin matrices of different rigidities. We present a focused position piece that illustrates the many different tests that a cell makes of its environment before it establishes mature matrix adhesions. When a fibroblast is pla...

journal_title：Biophysical journal

authors： Wolfenson H,Iskratsch T,Sheetz MP

更新日期：2014-12-02 00:00:00