The stiffness of the flagella of impaled bull sperm.

abstract：:During replication, the physical state of a virus is controlled by assembly and disassembly processes, when particles are put together and dismantled by cellular cues, respectively. A fundamental question has been how a cell can assemble an infectious virus, and dismantle a virus entering an uninfected cell and thereb...

journal_title：Biophysical journal

authors： Greber UF

更新日期：2014-06-03 00:00:00

abstract：:The current rapid expansion of biological knowledge offers a great opportunity to rationally engineer biological systems that respond to signals such as light and chemical inducers by producing specific proteins. Turning on and off the production of proteins on demand holds great promise for creating significant biote...

journal_title：Biophysical journal

authors： Tuttle LM,Salis H,Tomshine J,Kaznessis YN

更新日期：2005-12-01 00:00:00

abstract：:At present, fluorescence recovery after photobleaching (FRAP) data are interpreted using various types of reaction-diffusion (RD) models: the model type is usually fixed first, and corresponding model parameters are inferred subsequently. In this article, we describe what we believe to be a novel approach for RD model...

journal_title：Biophysical journal

authors： Mai J,Trump S,Ali R,Schiltz RL,Hager G,Hanke T,Lehmann I,Attinger S

更新日期：2011-03-02 00:00:00

abstract：:The depth of insertion of an antimicrobial peptide, protegrin-1 (PG-1), in lipid bilayers is investigated using solid-state NMR. Paramagnetic Mn(2+) ions bind to the surface of lipid bilayers and induce distance-dependent dipolar relaxation of nuclear spins. By comparing the signal dephasing of the peptide with that o...

journal_title：Biophysical journal

authors： Buffy JJ,Hong T,Yamaguchi S,Waring AJ,Lehrer RI,Hong M

更新日期：2003-10-01 00:00:00

abstract：:Transmissible spongiform encephalopathies are neurodegenerative diseases characterized by the accumulation of an abnormal isoform of the prion protein PrP(Sc). Its fragment 106-126 has been reported to maintain most of the pathological features of PrP(Sc), and a role in neurodegeneration has been proposed based on the...

journal_title：Biophysical journal

authors： Henriques ST,Pattenden LK,Aguilar MI,Castanho MA

更新日期：2008-08-01 00:00:00

abstract：:The raft hypothesis proposes that microdomains enriched in sphingolipids, cholesterol, and specific proteins are transiently formed to accomplish important cellular tasks. Equivocally, detergent-resistant membranes were initially assumed to be identical to membrane rafts, because of similarities between their composit...

journal_title：Biophysical journal

authors： Casadei BR,Domingues CC,de Paula E,Riske KA

更新日期：2014-06-03 00:00:00

abstract：:Stationary and nonstationary correlation-frequency analysis of heterodyne laser light scattering were utilized to make automated, on-line, objective measurements of tracheal ciliary beat frequency (CBF) in intact, anesthetized canines. The stationary correlation-frequency analysis laser light-scattering technique was ...

journal_title：Biophysical journal

authors： Chandra T,Yeates DB,Miller IF,Wong LB

更新日期：1994-03-01 00:00:00

abstract：:Surface charge in track-etched polyethylene terephthalate (PET) membranes with narrow pores has been probed with a fluorescent cationic dye (3,3'-diethyloxacarbocyanine iodide (diO-C2-(3))) using confocal microscopy. Staining of negatively charged PET membranes with diO-C2-(3) is a useful measure of surface charge for...

journal_title：Biophysical journal

authors： Bashford CL,Alder GM,Pasternak CA

更新日期：2002-04-01 00:00:00

abstract：:Two piezoelectric constants (polarization per unit stress, d=d'-id'', and polarization per unit strain, e=e'-ie''), the elastic constant, and dielectric constant are determined for oriented collagen at different hydration levels at 10 Hz from -150 to 50 degrees C. With no hydration (approximately 0% H2O), d' increases...

journal_title：Biophysical journal

authors： Fukada E,Ueda H,Rinaldi R

更新日期：1976-08-01 00:00:00

abstract：:The current article describes a new two-dimensional lambda-dynamics method to include proton tautomerism in continuous constant pH molecular dynamics (CPHMD) simulations. The two-dimensional lambda-dynamics framework is used to devise a tautomeric state titration model for the CPHMD simulations involving carboxyl and ...

journal_title：Biophysical journal

authors： Khandogin J,Brooks CL 3rd

更新日期：2005-07-01 00:00:00

abstract：:We used optical tweezers to analyze the effect of jasplakinolide and cyclodextrin on the force exerted by lamellipodia from developing growth cones (GCs) of isolated dorsal root ganglia (DRG) neurons. We found that 25 nM of jasplakinolide, which is known to inhibit actin filament turnover, reduced both the maximal exe...

journal_title：Biophysical journal

authors： Amin L,Ercolini E,Shahapure R,Migliorini E,Torre V

更新日期：2012-06-06 00:00:00

abstract：:Spin inversion transfer (SIT) NMR experiments are reported probing the thermodynamics and kinetics of interconversion of two folded forms of a GCN4-like leucine zipper near room temperature. The peptide is 13Calpha-labeled at position V9(a) and results are compared with prior findings for position L13(e). The SIT data...

journal_title：Biophysical journal

authors： d'Avignon DA,Bretthorst GL,Holtzer ME,Holtzer A

更新日期：1999-05-01 00:00:00

abstract：:Cationic lipid-DNA (CL-DNA) complexes are abundantly used in nonviral gene therapy clinical applications. Surface functionality is the next step in developing these complexes as competent, target-specific gene carriers. Poly(ethylene glycol) (PEG) is the natural choice to serve as a protective coat or act as a tether ...

journal_title：Biophysical journal

authors： Martin-Herranz A,Ahmad A,Evans HM,Ewert K,Schulze U,Safinya CR

更新日期：2004-02-01 00:00:00

abstract：:The demembranated (skinned) muscle fiber preparation is widely used to investigate muscle contraction because the intracellular ionic conditions can be precisely controlled. However, plasma membrane removal results in a loss of osmotic regulation, causing abnormal hydration of the myofilament lattice and its proteins....

journal_title：Biophysical journal

authors： Tanner BC,Farman GP,Irving TC,Maughan DW,Palmer BM,Miller MS

更新日期：2012-09-19 00:00:00

abstract：:A recent analysis of the normal modes of vibration of a ring formed by bringing together and sealing, with or without the addition of twist, the ends of rods that are straight when stress free is taken as the basis for a theory of the statistical thermodynamics of a canonical ensemble of DNA minicircles with specified...

journal_title：Biophysical journal

authors： Tobias I

更新日期：1998-05-01 00:00:00

abstract：:A newly developed microscope using acousto-optic tunable filters (AOTFs) was used to generate in vivo hemoglobin saturation (SO2) and oxygen tension (PO2) maps in the cerebral cortex of mice. SO2 maps were generated from the spectral analysis of reflected absorbance images collected at different wavelengths, and PO2 m...

journal_title：Biophysical journal

authors： Shonat RD,Wachman ES,Niu W,Koretsky AP,Farkas DL

更新日期：1997-09-01 00:00:00

abstract：:The subgel phases of unlabeled, specifically chain perdeuterated and specifically 13C = O labeled representative samples of the n-saturated diacylphosphatidylcholines were studied by Fourier-transform infrared spectroscopy. Our results indicate that the spectroscopic properties exhibited by the subgel phases of the lo...

journal_title：Biophysical journal

authors： Lewis RN,McElhaney RN

更新日期：1992-01-01 00:00:00

abstract：:Potentiometric titrations and surface potential measurements have been used to determine the intrinsic pKa values of both the carboxyl and amino groups of phosphatidylserine (PS) in mixed vesicles of PS and phosphatidylcholine (PC), and also of the amino group of phosphatidylethanolamine (PE) in mixed PE-PC vesicles. ...

journal_title：Biophysical journal

authors： Tsui FC,Ojcius DM,Hubbell WL

更新日期：1986-02-01 00:00:00

abstract：:Formamide-induced detubulation of rat ventricular myocytes was used to investigate the functional distribution of the Na/Ca exchanger (NCX) and Na/K-ATPase between the t-tubules and external sarcolemma. Detubulation resulted in a 32% decrease in cell capacitance, whereas cell volume was unchanged. Thus, the surface-to...

journal_title：Biophysical journal

authors： Despa S,Brette F,Orchard CH,Bers DM

更新日期：2003-11-01 00:00:00

abstract：:Two-dimensional electronic chirality-induced signals of excitons in the photosynthetic Fenna-Matthews-Olson complex from two species of green sulfur bacteria (Chlorobium tepidum and Prosthecochloris aestuarii) are compared. The spectra are predicted to provide sensitive probes of local protein environment of the const...

journal_title：Biophysical journal

authors： Voronine DV,Abramavicius D,Mukamel S

更新日期：2008-11-15 00:00:00

abstract：:A detailed analysis is made of the model system of two parallel cylindrical polyelectrolytes which contain ionizable groups on their surfaces and are immersed in an ionic bathing medium. The interaction between the cylinders is examined by considering the interplay between repulsive electrostatic forces and attractive...

journal_title：Biophysical journal

authors： Brenner SL,McQuarrie DA

更新日期：1973-04-01 00:00:00

abstract：:The observation of biological processes at the molecular scale in real time requires high spatial and temporal resolution. Magnetic tweezers are straightforward to implement, free of radiation or photodamage, and provide ample multiplexing capability, but their spatiotemporal resolution has lagged behind that of other...

journal_title：Biophysical journal

authors： Dulin D,Cui TJ,Cnossen J,Docter MW,Lipfert J,Dekker NH

更新日期：2015-11-17 00:00:00

abstract：:We investigate unfolding/folding force kinetics in DNA hairpins exhibiting two and three states with newly designed short dsDNA handles (29 bp) using optical tweezers. We show how the higher stiffness of the molecular setup moderately enhances the signal/noise ratio (SNR) in hopping experiments as compared to conventi...

journal_title：Biophysical journal

authors： Forns N,de Lorenzo S,Manosas M,Hayashi K,Huguet JM,Ritort F

更新日期：2011-04-06 00:00:00

abstract：:Purple photosynthetic bacteria harvest light using pigment-protein complexes which are often arranged in pseudo-organelles called chromatophores. A model of a chromatophore from Rhodospirillum photometricum was constructed based on atomic force microscopy data. Molecular-dynamics simulations and quantum-dynamics calcu...

journal_title：Biophysical journal

authors： Chandler DE,Strümpfer J,Sener M,Scheuring S,Schulten K

更新日期：2014-06-03 00:00:00

abstract：:We showed recently that the high-salt transition of poly[d(G-C)]. poly[d(G-C)] between B-DNA and Z-DNA (at [NaCl] = 2.25 M or [MgCl(2)] = 0.7 M) can be ascribed to the lesser electrostatic free energy of the B form, due to better immersion of the phosphates in the solution. This property was incorporated in cylindrica...

journal_title：Biophysical journal

authors： Guéron M,Demaret J,Filoche M

更新日期：2000-02-01 00:00:00

abstract：:The development of scanning probe techniques has made it possible to examine protein-protein interactions at the level of individual molecular pairs. A calibrated optical tweezers, along with immunoglobulin G (IgG)-coated polystyrene microspheres, has been used to detect individual surface-linked Staphylococcus protei...

journal_title：Biophysical journal

authors： Stout AL

更新日期：2001-06-01 00:00:00

abstract：:Cell motion is driven by interplay between the actin cytoskeleton and the cell adhesions in the front part of the cell. The actin network segregates into lamellipodium and lamellum, whereas the adhesion complexes are characteristically distributed underneath the actin system. Here, we suggest a computational model for...

journal_title：Biophysical journal

authors： Shemesh T,Bershadsky AD,Kozlov MM

更新日期：2012-04-18 00:00:00

abstract：:Sarcoplasmic reticulum (SR) Ca2+ ATPase was derivatized with 4,4'-diisothiocyanatostilbene-2,2'-sulfonic acid (DIDS), and complete enzyme inactivation was produced with a molecular stoichiometry of one DIDS per ATPase. It was determined by peptide analysis and sequencing that Lys492 and Lys515 were the ATPase residues...

journal_title：Biophysical journal

authors： Hua S,Inesi G

更新日期：1997-10-01 00:00:00

abstract：:Conductance noise measurement of the open states of alamethicin transmembrane channels reveals excess noise attributable to cooperative low-frequency molecular dynamics that can generate fluctuations approximately 1 A rms in the effective channel pore radius. Single-channel currents through both persistent and nonpers...

journal_title：Biophysical journal

authors： Mak DO,Webb WW

更新日期：1995-12-01 00:00:00

abstract：:This study presents what is, to our knowledge, a novel technique by means of which the ratio of the single gating charges of voltage-gated rat brain IIA (rBIIA) sodium and Shaker potassium ion channels was estimated. In the experiment, multiple tandems of enhanced green fluorescent protein were constructed and inserte...

journal_title：Biophysical journal

authors： Gamal El-Din TM,Grögler D,Lehmann C,Heldstab H,Greeff NG

更新日期：2008-08-01 00:00:00