Fluctuation of surface charge in membrane pores.

abstract：:The cytoskeleton is involved in numerous cellular processes such as migration, division, and contraction and provides the tracks for transport driven by molecular motors. Therefore, it is very important to quantify the mechanical behavior of the cytoskeletal filaments to get a better insight into cell mechanics and or...

journal_title：Biophysical journal

authors： Pallavicini C,Levi V,Wetzler DE,Angiolini JF,Benseñor L,Despósito MA,Bruno L

更新日期：2014-06-17 00:00:00

abstract：:An attempt was made to confirm previous reports of resonant-like dielectric absorption of plasmid DNA in aqueous solutions at 1-10 GHz. The dielectric properties of the sample were measured using an automatic network analyzer with two different techniques. One technique used an open-ended coaxial probe immersed in the...

journal_title：Biophysical journal

authors： Foster KR,Epstein BR,Gealt MA

更新日期：1987-09-01 00:00:00

abstract：:We have constructed a model of the upper part of the glycolysis in the pancreatic beta-cell. The model comprises the enzymatic reactions from glucokinase to glyceraldehyde-3-phosphate dehydrogenase (GAPD). Our results show, for a substantial part of the parameter space, an oscillatory behavior of the glycolysis for a ...

journal_title：Biophysical journal

authors： Westermark PO,Lansner A

更新日期：2003-07-01 00:00:00

abstract：:The most probable source of force generation in muscle fibers in the rotation of the myosin head when bound to actin. This laboratory has demonstrated that ATP induces microsecond rotational motions of spin-labeled myosin heads bound to actin (Berger, C. L. E. C. Svensson, and D. D. Thomas. 1989. Proc. Natl. Acad. Sci...

journal_title：Biophysical journal

authors： Ostap EM,Thomas DD

更新日期：1991-06-01 00:00:00

abstract：:Fundamental mechanisms governing cell size control and homeostasis are still poorly understood. The relationship between sizes at division and birth in single cells is used as a metric to categorize the basis of size homeostasis. Cells dividing at a fixed size regardless of birth size (sizer) are expected to show a di...

journal_title：Biophysical journal

authors： Facchetti G,Knapp B,Chang F,Howard M

更新日期：2019-11-05 00:00:00

abstract：:Bax and Bid are proapoptotic proteins of the Bcl-2 family that regulate the release of apoptogenic factors from mitochondria. Although they localize constitutively in the cytoplasm, their apoptotic function is exerted at the mitochondrial outer membrane, and is related to their ability to form transbilayer pores. Here...

journal_title：Biophysical journal

authors： García-Sáez AJ,Coraiola M,Dalla Serra M,Mingarro I,Menestrina G,Salgado J

更新日期：2005-06-01 00:00:00

abstract：:Stationary and nonstationary correlation-frequency analysis of heterodyne laser light scattering were utilized to make automated, on-line, objective measurements of tracheal ciliary beat frequency (CBF) in intact, anesthetized canines. The stationary correlation-frequency analysis laser light-scattering technique was ...

journal_title：Biophysical journal

authors： Chandra T,Yeates DB,Miller IF,Wong LB

更新日期：1994-03-01 00:00:00

abstract：:During postnatal development, major changes in mechanical properties of skeletal muscle occur. We investigated passive properties of skeletal muscle in mice and rabbits that varied in age from 1 day to approximately 1 year. Neonatal skeletal muscle expressed large titin isoforms directly after birth, followed by a gra...

journal_title：Biophysical journal

authors： Ottenheijm CA,Knottnerus AM,Buck D,Luo X,Greer K,Hoying A,Labeit S,Granzier H

更新日期：2009-10-21 00:00:00

abstract：:It has been hypothesized, from earlier studies on single-tether extraction from individual leukocytes and human umbilical vein endothelial cells, that during rolling of leukocytes on the endothelium, simultaneous extraction of membrane nanotubes (tethers) occurs, resulting in enhancement of the force decrease on the a...

journal_title：Biophysical journal

authors： Girdhar G,Shao JY

更新日期：2007-12-01 00:00:00

abstract：:The participation of amino-terminal domains in human ether-a-go-go (eag)-related gene (HERG) K(+) channel gating was studied using deleted channel variants expressed in Xenopus oocytes. Selective deletion of the HERG-specific sequence (HERG Delta138-373) located between the conserved initial amino terminus (the eag or...

journal_title：Biophysical journal

authors： Viloria CG,Barros F,Giráldez T,Gómez-Varela D,de la Peña P

更新日期：2000-07-01 00:00:00

abstract：:Ca2+ release from the endoplasmic reticulum (ER) contributes to Ca2+ transients in frog sympathetic ganglion neurons. Here we use video-rate confocal fluo-4 fluorescence imaging to show that single action potentials reproducibly trigger rapidly rising Ca2+ transients at 1-3 local hot spots within the peripheral ER-ric...

journal_title：Biophysical journal

authors： Cseresnyés Z,Schneider MF

更新日期：2004-01-01 00:00:00

abstract：:Equations describing the movement of sugars during induced uphill transport were derived on the assumption of a simple carrier transport mechanism and subjected to experimental verification. Since there was good agreement between the experimental points and the theoretical curves, no changes in the original postulates...

journal_title：Biophysical journal

authors： Miller DM

更新日期：1965-07-01 00:00:00

abstract：:The results of a 3-ns molecular dynamics simulation of the dodecamer duplex d(TATGGATCCATA)(2) recognized by the BamHI endonuclease are presented here. The DNA has been simulated as a flexible molecule using an AMBER force field and the Ewald summation method, which eliminates the undesired effects of truncation and p...

journal_title：Biophysical journal

authors： Castrignanò T,Chillemi G,Desideri A

更新日期：2000-09-01 00:00:00

abstract：:Halorhodopsin (HR) and sensory rhodopsin (SR) have been regenerated with retinal analogues that are covalently locked in the 6-s-cis or 6-s-trans conformations. Both pigments regenerate more completely with the locked 6-s-trans retinal and produce analogue pigments with absorption maxima (577 nm for HR and 592 nm for ...

journal_title：Biophysical journal

authors： Baselt DR,Fodor SP,van der Steen R,Lugtenburg J,Bogomolni RA,Mathies RA

更新日期：1989-01-01 00:00:00

abstract：:Formation of amyloid oligomers, the most toxic species of amyloids in degenerative diseases, is critically coupled to the interplay with surrounding water. The hydrophobic force driving the oligomerization causes water removal from interfaces, changing the surface-hydration properties. Here, we show that such effects ...

journal_title：Biophysical journal

authors： Walton JH,Berry RS,Despa F

更新日期：2011-05-04 00:00:00

abstract：:Using polarography and suitable mathematical analysis, the oxygen permeability of an individual layer of a bilayered tissue may be determined if the oxygen consumption and permeability of the other layer are known. Results obtained on rabbit cornea indicate that the permeability of the epithelium is about four times t...

journal_title：Biophysical journal

authors： Freeman RD,Fatt I

更新日期：1972-03-01 00:00:00

abstract：:Dim-light vision is mediated by retinal rod cells. Rhodopsin (R), a G-protein-coupled receptor, switches to its active form (R(∗)) in response to absorbing a single photon and activates multiple copies of the G-protein transducin (G) that trigger further downstream reactions of the phototransduction cascade. The class...

journal_title：Biophysical journal

authors： Schöneberg J,Heck M,Hofmann KP,Noé F

更新日期：2014-09-02 00:00:00

abstract：:In the rabbit corneal stroma, the swelling pressure, P, has been reported to have an anomalous (negative) temperature coefficient, alpha P, contradicting traditional Donnan swelling theory. A parallel-plate, diffuse double layer Gouy-Chapman model was used to resolve this discrepancy. The present model incorporates th...

journal_title：Biophysical journal

authors： Kwok LS,Klyce SD

更新日期：1990-03-01 00:00:00

abstract：:The effects of the Ca(2+)-induced Ca2+ release blocker procaine on individual sarcoplasmic reticulum Ca2+ release channels have been examined in planar lipid bilayers. Procaine did not reduce the single channel conductance nor appreciably shorten the mean open times of the channel; rather, it increased the longest clo...

journal_title：Biophysical journal

authors： Zahradníková A,Palade P

更新日期：1993-04-01 00:00:00

abstract：:Uracil DNA Glycosylase (UNG2) is the primary enzyme in humans that prevents the stable incorporation of deoxyuridine monophosphate into DNA in the form of U/A basepairs. During S-phase, UNG2 remains associated with the replication fork through its interactions with two proteins, Proliferating Cell Nuclear Antigen (PCN...

journal_title：Biophysical journal

authors： Weiser BP,Stivers JT,Cole PA

更新日期：2017-07-25 00:00:00

abstract：:A dissipative system is approximated by a nonlinear rate equation: Z congruent to K1Z - K2Z3 (K2 greater than 0), in which the right side is derived from -delta G/delta Z of Taylor's series of the thermodynamic potential given by Gibbs' function G(Tc, Pc) (Z) at about the critical point C(Tc, Pc) of the control variab...

journal_title：Biophysical journal

authors： Shirane K,Tokimoto T,Yamaguchi Y

更新日期：1990-01-01 00:00:00

abstract：:A new method for the investigation of ion translocating membrane proteins is presented. Protein containing membrane fragments or vesicles are adsorbed to a solid supported membrane. The solid supported membrane consists of a lipid monolayer on a gold evaporated or gold sputtered glass substrate which is coated with a ...

journal_title：Biophysical journal

authors： Seifert K,Fendler K,Bamberg E

更新日期：1993-02-01 00:00:00

abstract：:Pressure versus distance relations have been obtained for solid (gel) and neat (liquid-crystalline) phase uncharged lipid bilayers by the use of x-ray diffraction analysis of osmotically stressed monoglyceride aqueous dispersions and multilayers. For solid phase monoelaidin bilayers, the interbilayer repulsive pressur...

journal_title：Biophysical journal

authors： McIntosh TJ,Magid AD,Simon SA

更新日期：1989-05-01 00:00:00

abstract：:Synchrotron x-ray diffraction, differential scanning calorimetry, and electron spin resonance spectroscopy have been employed to characterize a quasicrystalline phase formed in aqueous dispersions of binary mixtures of glucocerebroside and palmitoyloleoylphosphatidylethanolamine. Small- and wide-angle x-ray scattering...

journal_title：Biophysical journal

authors： Feng Y,Rainteau D,Chachaty C,Yu ZW,Wolf C,Quinn PJ

更新日期：2004-04-01 00:00:00

abstract：:The rate of muscle force redevelopment after release-restretch protocols has previously been interpreted using a simple two-state cross-bridge cycling model with rate constants for transitions between non-force-bearing and force-bearing states, f, and between force-bearing and non-force-bearing states, g. Changes in t...

journal_title：Biophysical journal

authors： Campbell K

更新日期：1997-01-01 00:00:00

abstract：:Model compound studies in the literature show how Hofmeister ion interactions affect protein stability. Although model compound results are typically obtained as salting-out constants, they can be used to find out how the interactions affect protein stability. The null point in the Hofmeister series, which divides pro...

journal_title：Biophysical journal

authors： Baldwin RL

更新日期：1996-10-01 00:00:00

abstract：:The mitochondrial channel, VDAC, forms large (3 nm in diameter) aqueous pores through membranes. We measured ATP flow (using the luciferin/luciferase method) through these channels after reconstitution into planar phospholipid membranes. In the open state of VDAC, as many as 2 x 10(6) ATP molecules can flow through on...

journal_title：Biophysical journal

authors： Rostovtseva T,Colombini M

更新日期：1997-05-01 00:00:00

abstract：:Filopodia perform cellular functions such as environmental sensing or cell motility, but they also grab for particles and withdraw them leading to an increased efficiency of phagocytic uptake. Remarkably, withdrawal of micron-sized particles is also possible without noticeable movements of the filopodia. Here, we demo...

journal_title：Biophysical journal

authors： Kohler F,Rohrbach A

更新日期：2015-05-05 00:00:00

abstract：:A phospholipid chelate complexed with ytterbium (DMPE-DTPA:Yb3+) is shown to be readily incorporated into a model membrane system, which may then be aligned in a magnetic field such that the average bilayer normal lies along the field. This so-called positively ordered smectic phase, whose lipids consist of less than ...

journal_title：Biophysical journal

authors： Prosser RS,Volkov VB,Shiyanovskaya IV

更新日期：1998-11-01 00:00:00

abstract：:Understanding the mechanism of saturated fatty acid-induced hepatocyte toxicity may provide insight into cures for diseases such as obesity-associated cirrhosis. Trehalose, a nonreducing disaccharide shown to protect proteins and cellular membranes from inactivation or denaturation caused by different stress condition...

journal_title：Biophysical journal

authors： Leekumjorn S,Wu Y,Sum AK,Chan C

更新日期：2008-04-01 00:00:00