Abstract：
:The development of scanning probe techniques has made it possible to examine protein-protein interactions at the level of individual molecular pairs. A calibrated optical tweezers, along with immunoglobulin G (IgG)-coated polystyrene microspheres, has been used to detect individual surface-linked Staphylococcus protein A (SpA) molecules and to characterize the strength of the noncovalent IgG-SpA bond. Microspheres containing, on average, less than one IgG per contact area were held in the optical trap while an SpA-coated substrate was scanned beneath them at a distance of approximately 50 nm. This geometry allows the trapped bead to make contact with the surface, from bond formation to rupture, and results in an enhancement of the force applied to a bond due to leverage supplied by the bead itself. Experiments yielded median single-bond rupture forces from 25 to 44 pN for IgG from four mammalian species, in general agreement with predictions based on free energies of association obtained from solution equilibrium constants.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Stout ALdoi
10.1016/S0006-3495(01)76263-7subject
Has Abstractpub_date
2001-06-01 00:00:00pages
2976-86issue
6eissn
0006-3495issn
1542-0086pii
S0006-3495(01)76263-7journal_volume
80pub_type
杂志文章abstract：:Protein aggregation is linked to more than 30 human pathologies, including Alzheimer's and Parkinson's diseases. Since small oligomers that form at the beginning of the fibrillation process probably are the most toxic elements, therapeutic strategies involving fibril fragmentation could be detrimental. An alternative ...
journal_title：Biophysical journal
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journal_title：Biophysical journal
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journal_title：Biophysical journal
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pub_type： 杂志文章
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更新日期：1983-01-01 00:00:00
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journal_title：Biophysical journal
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journal_title：Biophysical journal
pub_type： 杂志文章
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(86)83692-X
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journal_title：Biophysical journal
pub_type： 杂志文章
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更新日期：1971-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：1997-01-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
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更新日期：2001-01-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
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更新日期：2015-03-10 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2008.09.061
更新日期：2009-03-04 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
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更新日期：1993-05-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2011.08.055
更新日期：2011-10-19 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(89)82730-4
更新日期：1989-11-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(01)75920-6
更新日期：2001-11-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(02)73925-8
更新日期：2002-09-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
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更新日期：2004-01-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
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更新日期：2015-04-07 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2012.11.3827
更新日期：2013-01-08 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(04)74190-9
更新日期：2004-02-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2012.08.009
更新日期：2012-09-05 00:00:00
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journal_title：Biophysical journal
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doi：10.1016/j.bpj.2020.03.009
更新日期：2020-04-21 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.105.072017
更新日期：2006-04-15 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2020.06.001
更新日期：2020-07-07 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(93)81039-7
更新日期：1993-07-01 00:00:00
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journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.105.073569
更新日期：2006-06-01 00:00:00
abstract：:Morphogenesis of epithelial tissues requires tight spatiotemporal coordination of cell shape changes. In vivo, many tissue-scale shape changes are driven by pulsatile contractions of intercellular junctions, which are rectified to produce irreversible deformations. The functional role of this pulsatory ratchet and its...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2019.09.027
更新日期：2019-11-05 00:00:00
