Detection and characterization of individual intermolecular bonds using optical tweezers.


:The development of scanning probe techniques has made it possible to examine protein-protein interactions at the level of individual molecular pairs. A calibrated optical tweezers, along with immunoglobulin G (IgG)-coated polystyrene microspheres, has been used to detect individual surface-linked Staphylococcus protein A (SpA) molecules and to characterize the strength of the noncovalent IgG-SpA bond. Microspheres containing, on average, less than one IgG per contact area were held in the optical trap while an SpA-coated substrate was scanned beneath them at a distance of approximately 50 nm. This geometry allows the trapped bead to make contact with the surface, from bond formation to rupture, and results in an enhancement of the force applied to a bond due to leverage supplied by the bead itself. Experiments yielded median single-bond rupture forces from 25 to 44 pN for IgG from four mammalian species, in general agreement with predictions based on free energies of association obtained from solution equilibrium constants.


Biophys J


Biophysical journal


Stout AL




Has Abstract


2001-06-01 00:00:00














  • Specific antigen/antibody interactions measured by force microscopy.

    abstract::Molecular recognition between biotinylated bovine serum albumin and polyclonal, biotin-directed IG antibodies has been measured directly under various buffer conditions using an atomic force microscope (AFM). It was found that even highly structured molecules such as IgG antibodies preserve their specific affinity to ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Dammer U,Hegner M,Anselmetti D,Wagner P,Dreier M,Huber W,Güntherodt HJ

    更新日期:1996-05-01 00:00:00

  • An intramolecular hydrogen bond with large proton polarizability within the head group of phosphatidylserine. An infrared investigation.

    abstract::Films of O-phospho-L-serine-P-ethylester (PSE) were studied by infrared spectroscopy. PSE films were studied pure and as 1:1 mixture with LiOH, NaOH, KOH, and Ca(OH)2 as a function of the degree of hydration. The same investigations were performed if (L-glu)n was added to the system (ratio 1:1, PSE/glu residue). In th...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Leberle K,Kempf I,Zundel G

    更新日期:1989-04-01 00:00:00

  • Blocking dynamics of the SMR transporter EmrE impairs efflux activity.

    abstract::EmrE is a small multidrug resistance transporter that has been well studied as a model for secondary active transport. Because transport requires the protein to convert between at least two states open to opposite sides of the membrane, it is expected that blocking these conformational transitions will prevent transpo...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Dutta S,Morrison EA,Henzler-Wildman KA

    更新日期:2014-08-05 00:00:00

  • Microsecond unfolding kinetics of sheep prion protein reveals an intermediate that correlates with susceptibility to classical scrapie.

    abstract::The microsecond folding and unfolding kinetics of ovine prion proteins (ovPrP) were measured under various solution conditions. A fragment comprising residues 94-233 of the full-length ovPrP was studied for four variants with differing susceptibilities to classical scrapie in sheep. The observed biexponential unfoldin...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Chen KC,Xu M,Wedemeyer WJ,Roder H

    更新日期:2011-09-07 00:00:00

  • Dielectric Relaxation of Molecules with Fluctuating Dipole Moment.

    abstract::When a dissolved macromolecule is in chemical equilibrium with a free ionic species, the charge configuration, and hence the dipole vector, of the macromolecule is fluctuating. Expressions for the static dielectric constant and the relaxation spectrum of such a mixture are here derived in terms of the components of th...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Scheider W

    更新日期:1965-09-01 00:00:00

  • Phosphorus-31 nuclear magnetic resonance spectroscopy of toad retina.

    abstract::Phosphorus-31 nuclear magnetic resonance (31P-NMR) spectra were obtained from living toad retinae and toad retinal extracts at 4 degrees C. Several phosphorus metabolites--nucleoside di- and triphosphates (NTP), phosphocreatine, phosphodiesters, inorganic phosphate, and phosphomonoesters--were identified from the spec...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Apte DV,Koutalos Y,McFarlane DK,Dawson MJ,Ebrey TG

    更新日期:1989-09-01 00:00:00

  • A new technique for calculating individual dermal fibroblast contractile forces generated within collagen-GAG scaffolds.

    abstract::Cell-mediated contraction plays a critical role in many physiological and pathological processes, notably organized contraction during wound healing. Implantation of an appropriately formulated (i.e., mean pore size, chemical composition, degradation rate) three-dimensional scaffold into an in vivo wound site effectiv...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Harley BA,Freyman TM,Wong MQ,Gibson LJ

    更新日期:2007-10-15 00:00:00

  • Protein fibrillation lag times during kinetic inhibition.

    abstract::Protein aggregation is linked to more than 30 human pathologies, including Alzheimer's and Parkinson's diseases. Since small oligomers that form at the beginning of the fibrillation process probably are the most toxic elements, therapeutic strategies involving fibril fragmentation could be detrimental. An alternative ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Pagano RS,López Medus M,Gómez GE,Couto PM,Labanda MS,Landolfo L,D'Alessio C,Caramelo JJ

    更新日期:2014-08-05 00:00:00

  • Multi-step fibrinogen binding to the integrin (alpha)IIb(beta)3 detected using force spectroscopy.

    abstract::The regulated ability of integrin alphaIIbbeta3 to bind fibrinogen plays a crucial role in platelet aggregation and hemostasis. We have developed a model system based on laser tweezers, enabling us to measure specific rupture forces needed to separate single receptor-ligand complexes. First of all, we performed a thor...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Litvinov RI,Bennett JS,Weisel JW,Shuman H

    更新日期:2005-10-01 00:00:00

  • Mechanisms of propagation of intercellular calcium waves in arterial smooth muscle cells.

    abstract::In rat mesenteric arteries, smooth muscle cells exhibit intercellular calcium waves in response to local phenylephrine stimulation. These waves have a velocity of approximately 20 cells/s and a range of approximately 80 cells. We analyze these waves in a theoretical model of a population of coupled smooth muscle cells...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Koenigsberger M,Seppey D,Bény JL,Meister JJ

    更新日期:2010-07-21 00:00:00

  • X-ray scattering from labeled membranes.

    abstract::We present a new method for the determination of structural parameters in biological membranes. Recording the continuous scattering of heavy-atom labeled membranes and applying elementary Fourier methods we obtain the scattering of the heavy-atom distribution alone. The details of this distribution are explored by dev...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Stamatoff J,Bilash T,Ching Y,Eisenberger P

    更新日期:1979-12-01 00:00:00

  • A method for anchoring round shaped cells for atomic force microscope imaging.

    abstract::More and more researchers are interested in imaging living (Henderson, 1994) or fixed cells in their natural environment using the atomic force microscope (AFM). However, the AFM tip interacts strongly with the sample, and its z range freedom is limited to a few micrometers. This means that the cells to be imaged have...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Kasas S,Ikai A

    更新日期:1995-05-01 00:00:00

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    abstract::Cytochrome c3 (cyt c3) can mediate electron transport across phosphatidylcholine (PC)/cardiolipin (CL) and PC/phosphatidylglycerol (PG) membranes. A two-molecule process is involved in the electron transport across PC/CL membranes in the liquid-crystalline state. In contrast, a single-molecule process dominates the el...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Kim SM,Yamamoto T,Todokoro Y,Takayama Y,Fujiwara T,Park JS,Akutsu H

    更新日期:2006-01-15 00:00:00

  • Photoreverse reaction dynamics of octopus rhodopsin.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Inoue K,Tsuda M,Terazima M

    更新日期:2007-05-15 00:00:00

  • Extraction of Thick Filaments in Individual Sarcomeres Affects Force Production by Single Myofibrils.

    abstract::It has been accepted that the force produced by a skeletal muscle myofibril depends on its cross-sectional area but not on the number of active sarcomeres because they are arranged in series. However, a previous study performed by our group showed that blocking actomyosin interactions within an activated myofibril and...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Mendoza AC,Rassier DE

    更新日期:2020-04-21 00:00:00

  • Contractile mechanisms in flagella.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Rikmenspoel R

    更新日期:1971-05-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Lee H,Kandasamy SK,Larson RG

    更新日期:2005-12-01 00:00:00

  • Random hydrolysis controls the dynamic instability of microtubules.

    abstract::Uncovering mechanisms that control the dynamics of microtubules is fundamental for our understanding of multiple cellular processes such as chromosome separation and cell motility. Building on previous theoretical work on the dynamic instability of microtubules, we propose here a stochastic model that includes all rel...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Padinhateeri R,Kolomeisky AB,Lacoste D

    更新日期:2012-03-21 00:00:00

  • Interaction of verapamil with lipid membranes and P-glycoprotein: connecting thermodynamics and membrane structure with functional activity.

    abstract::Verapamil and amlodipine are calcium ion influx inhibitors of wide clinical use. They are partially charged at neutral pH and exhibit amphiphilic properties. The noncharged species can easily cross the lipid membrane. We have measured with solid-state NMR the structural changes induced by verapamil upon incorporation ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Meier M,Blatter XL,Seelig A,Seelig J

    更新日期:2006-10-15 00:00:00

  • Analysis of a 10-ns molecular dynamics simulation of mouse acetylcholinesterase.

    abstract::A 10-ns molecular dynamics simulation of mouse acetylcholinesterase was analyzed, with special attention paid to the fluctuation in the width of the gorge and opening events of the back door. The trajectory was first verified to ensure its stability. We defined the gorge proper radius as the measure for the extent of ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Tai K,Shen T,Börjesson U,Philippopoulos M,McCammon JA

    更新日期:2001-08-01 00:00:00

  • Conformation of reconstituted mononucleosomes and effect of linker histone H1 binding studied by scanning force microscopy.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Kepert JF,Tóth KF,Caudron M,Mücke N,Langowski J,Rippe K

    更新日期:2003-12-01 00:00:00

  • Osmotic compression and stiffness changes in relaxed skinned cardiac myocytes in PVP-40 and dextran T-500.

    abstract::Sarcomere lengths, cell widths, indices of stiffness, and striation pattern uniformity were determined from radially compressed isolated adult cardiac myocytes from the rat. Single cells were bathed in a series of relaxing solutions containing 0-15% concentrations of nonpenetrating long chain polymers PVP-40 and dextr...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Roos KP,Brady AJ

    更新日期:1990-11-01 00:00:00

  • Molecular dynamics simulation of the M2 helices within the nicotinic acetylcholine receptor transmembrane domain: structure and collective motions.

    abstract::Multiple nanosecond duration molecular dynamics simulations were performed on the transmembrane region of the Torpedo nicotinic acetylcholine receptor embedded within a bilayer mimetic octane slab. The M2 helices and M2-M3 loop regions were free to move, whereas the outer (M1, M3, M4) helix bundle was backbone restrai...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Hung A,Tai K,Sansom MS

    更新日期:2005-05-01 00:00:00

  • Benefits and Limitations of Low-kV Macromolecular Imaging of Frozen-Hydrated Biological Samples.

    abstract::Object contrast is one of the most important parameters of macromolecular imaging. Low-voltage transmission electron microscopy has shown an increased atom contrast for carbon materials, indicating that amplitude contrast contributions increase at a higher rate than phase contrast and inelastic scattering. Here, we st...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Majorovits E,Angert I,Kaiser U,Schröder RR

    更新日期:2016-02-23 00:00:00

  • Single-molecule force spectroscopy of cartilage aggrecan self-adhesion.

    abstract::We investigated self-adhesion between highly negatively charged aggrecan macromolecules extracted from bovine cartilage extracellular matrix by performing atomic force microscopy (AFM) imaging and single-molecule force spectroscopy (SMFS) in saline solutions. By controlling the density of aggrecan molecules on both th...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Harder A,Walhorn V,Dierks T,Fernàndez-Busquets X,Anselmetti D

    更新日期:2010-11-17 00:00:00

  • High-resolution AFM of membrane proteins directly incorporated at high density in planar lipid bilayer.

    abstract::The heterologous expression and purification of membrane proteins represent major limitations for their functional and structural analysis. Here we describe a new method of incorporation of transmembrane proteins in planar lipid bilayer starting from 1 pmol of solubilized proteins. The principle relies on the direct i...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Milhiet PE,Gubellini F,Berquand A,Dosset P,Rigaud JL,Le Grimellec C,Lévy D

    更新日期:2006-11-01 00:00:00

  • Monolayers of a model anesthetic-binding membrane protein: formation, characterization, and halothane-binding affinity.

    abstract::hbAP0 is a model membrane protein designed to possess an anesthetic-binding cavity in its hydrophilic domain and a cation channel in its hydrophobic domain. Grazing incidence x-ray diffraction shows that hbAP0 forms four-helix bundles that are vectorially oriented within Langmuir monolayers at the air-water interface....

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Churbanova IY,Tronin A,Strzalka J,Gog T,Kuzmenko I,Johansson JS,Blasie JK

    更新日期:2006-05-01 00:00:00

  • Evidence for ligand-induced conformational changes in proteins from phosphorescence spectroscopy.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Li Z,Galley WC

    更新日期:1989-08-01 00:00:00

  • Structure and activation dynamics of RBL-2H3 cells observed with scanning force microscopy.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Braunstein D,Spudich A

    更新日期:1994-05-01 00:00:00

  • Regimes of Complex Lipid Bilayer Phases Induced by Cholesterol Concentration in MD Simulation.

    abstract::Cholesterol is essential to the formation of phase-separated lipid domains in membranes. Lipid domains can exist in different thermodynamic phases depending on the molecular composition and play significant roles in determining structure and function of membrane proteins. We investigate the role of cholesterol in the ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Pantelopulos GA,Straub JE

    更新日期:2018-12-04 00:00:00