Effect of cholesterol on structural and dynamic properties of tripalmitoyl glyceride. A high-pressure infrared spectroscopic study.

abstract：:Mössbauer spectra of anhydrohemoglobin establish the existence of two ferrous iron spin states in anhydrohemoglobin. Magnetic susceptibility measurements show that anhydrohemoglobin is paramagnetic and has an effective magnetic moment per iron atom of 3.5+/-0.1mu(B) at low temperatures. In conjunction with the suscept...

journal_title：Biophysical journal

authors： Grant RW,Cape JA,Gonser U,Topol LE,Saltman P

更新日期：1967-11-01 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopy was used to monitor secondary structural changes associated with binding of bovine prothrombin and prothrombin fragment 1 to acidic lipid membranes. Prothrombin and prothrombin fragment 1 were examined under four different conditions: in the presence of (a) Na2EDTA, (b) 5...

journal_title：Biophysical journal

authors： Wu JR,Lentz BR

更新日期：1991-07-01 00:00:00

abstract：:Calcium ions act presynaptically to modulate synaptic strength and to trigger neurotransmitter release. Here we detect stimulus-evoked changes in residual free calcium ([Ca2+]i) in rat cerebellar granule cell presynaptic terminals. Granule cell axons, known as parallel fibers, and their associated boutons, were labele...

journal_title：Biophysical journal

authors： Regehr WG,Atluri PP

更新日期：1995-05-01 00:00:00

abstract：:Chemotactic effects of dissolved oxygen on motions of Escherichia coli in a motility buffer solution have been studied by measurements of quasielastic light scattering. Under conditions where the bacteria form a sharp band in an oxygen concentrations gradient created by their metabolism, components of motions along th...

journal_title：Biophysical journal

authors： Wang PC,Chen SH

更新日期：1981-10-01 00:00:00

abstract：:The salt dependence of the binding free energy of five protein-protein hetero-dimers and two homo-dimers/tetramers was calculated from numerical solutions to the Poisson-Boltzmann equation. Overall, the agreement with experimental values is very good. In all cases except one involving the highly charged lactoglobulin ...

journal_title：Biophysical journal

authors： Bertonati C,Honig B,Alexov E

更新日期：2007-03-15 00:00:00

abstract：:Understanding exposure thresholds for the response of biological systems to extremely low frequency (ELF) electric and magnetic fields is a fundamental problem of long-standing interest. We consider a two-state model for voltage-gated channels in the membrane of an isolated elongated cell (Lcell = 1 mm; rcell = 25 mic...

journal_title：Biophysical journal

authors： Weaver JC,Vaughan TE,Adair RK,Astumian RD

更新日期：1998-11-01 00:00:00

abstract：:Interactions between the graft copolymer poly(L-lysine)-g-poly(ethylene glycol), PLL-g-PEG, and two kinds of surface-supported lipidic systems (supported phospholipid bilayers and supported vesicular layers) were investigated by a combination of microscopic and spectroscopic techniques. It was found that the applicati...

journal_title：Biophysical journal

authors： Rossetti FF,Reviakine I,Csúcs G,Assi F,Vörös J,Textor M

更新日期：2004-09-01 00:00:00

abstract：:The determination of intracellular protein concentrations is a prerequisite for understanding protein interaction networks in systems biology. Today, protein quantification is based either on mass spectrometry, which requires large cell numbers and sophisticated measurement protocols, or on quantitative Western blotti...

journal_title：Biophysical journal

authors： Chung YD,Sinzinger MD,Bovee-Geurts P,Krause M,Dinkla S,Joosten I,Koopman WJ,Adjobo-Hermans MJ,Brock R

更新日期：2011-12-07 00:00:00

abstract：:We investigate lateral organization of lipid domains in vesicles versus supported membranes and monolayers. The lipid mixtures used are predominantly DOPC/DPPC/Chol and DOPC/BSM/Chol, which have been previously shown to produce coexisting liquid phases in vesicles and monolayers. In a monolayer at an air-water interfa...

journal_title：Biophysical journal

authors： Stottrup BL,Veatch SL,Keller SL

更新日期：2004-05-01 00:00:00

abstract：:Actin is ubiquitous globular protein that polymerizes into filaments and forms networks that participate in the force generation of eukaryotic cells. Such forces are used for cell motility, cytokinesis, and tissue remodeling. Among those actin networks, we focus on the actin cortex, a dense branched network beneath th...

journal_title：Biophysical journal

authors： Bussonnier M,Carvalho K,Lemière J,Joanny JF,Sykes C,Betz T

更新日期：2014-08-19 00:00:00

abstract：:We investigated whether transient step reductions in divalent cations would produce detectable changes in neuronal excitability similar to those reported in the total absence of divalent cations. Using cultured chick dorsal root ganglion cells as a model system, our results indicate that a step reduction in divalent c...

journal_title：Biophysical journal

authors： Hablitz JJ,Heinemann U,Lux HD

更新日期：1986-10-01 00:00:00

abstract：:Using CD spectroscopy, guanine tetraplex formation was studied with short DNA fragments in which cytosine residues were systematically added to runs of guanine either at the 5' or 3' ends. Potassium cations induced the G-tetraplex more easily with fragments having the guanine run at the 5' end, which is just an opposi...

journal_title：Biophysical journal

authors： Penázová H,Vorlicková M

更新日期：1997-10-01 00:00:00

abstract：:It was shown previously that a significant fraction of the myosin crossbridges is attached to actin in the skinned rabbit psoas fibers under relaxed conditions at low ionic strength and low temperature (Brenner, B., M. Schoenberg, J. M. Chalovich, L. E. Greene, and E. Eisenberg. 1982. Proc. Natl. Acad. Sci. USA. 79:72...

journal_title：Biophysical journal

authors： Yu LC,Brenner B

更新日期：1989-03-01 00:00:00

abstract：:Dendritic shaft (Zd) and spine (Zsp) input impedances were computed numerically for sites on hippocampal neurons, using a segmental format of cable calculations. The Zsp values for a typical spine appended onto a dendritic shaft averaged less than 2% higher than the Zd values for the adjacent dendritic shaft. Spine sy...

journal_title：Biophysical journal

authors： Turner DA

更新日期：1984-07-01 00:00:00

abstract：:EmrE is a small multidrug resistance transporter that has been well studied as a model for secondary active transport. Because transport requires the protein to convert between at least two states open to opposite sides of the membrane, it is expected that blocking these conformational transitions will prevent transpo...

journal_title：Biophysical journal

authors： Dutta S,Morrison EA,Henzler-Wildman KA

更新日期：2014-08-05 00:00:00

abstract：:The structural properties of a crucial transmembrane helix for proton translocation in vacuolar ATPase are studied using double site-directed spin-labeling combined with electron spin resonance (ESR) (or electron paramagnetic resonance) and circular dichroism spectroscopy in sodium dodecyl sulfate micelles. For this p...

journal_title：Biophysical journal

authors： Vos WL,Vermeer LS,Hemminga MA

更新日期：2007-01-01 00:00:00

abstract：:Intracellular recordings of cortical neurons in vivo display intense subthreshold membrane potential (V(m)) activity. The power spectral density of the V(m) displays a power-law structure at high frequencies (>50 Hz) with a slope of approximately -2.5. This type of frequency scaling cannot be accounted for by traditio...

journal_title：Biophysical journal

authors： Bédard C,Destexhe A

更新日期：2008-02-15 00:00:00

abstract：:Antisense oligonucleotides present a powerful means to inhibit expression of specific genes, but their effectiveness is limited by factors including cellular delivery, biochemical attack, and poor binding to target. We have developed a systems model of the processes required for an antisense oligonucleotide to enter, ...

journal_title：Biophysical journal

authors： Roth CM

更新日期：2005-10-01 00:00:00

abstract：:The strength of receptor-mediated cell adhesion is directly controlled by the mechanism of cohesive failure between the cell surface and underlying substrate. Unbinding can occur either at the locus of the specific bond or within the bilayer, which results in tearing the hydrophobic anchors from the membrane interior....

journal_title：Biophysical journal

authors： Leckband D,Müller W,Schmitt FJ,Ringsdorf H

更新日期：1995-09-01 00:00:00

abstract：:Strain B(8-11) has been found to be very sensitive to postirradiation DNA degradation. Up to 98% of the DNA is degraded at optimum doses. The amount of residual DNA correlates with the retention of colony-forming ability (CFA). Studies of rates of degradation as a function of dose agree with the concept that a degradi...

journal_title：Biophysical journal

authors： Pollard EC,Tilberg A

更新日期：1972-02-01 00:00:00

abstract：:The redox-driven proton pump cytochrome c oxidase is that enzymatic machinery of the respiratory chain that transfers electrons from cytochrome c to molecular oxygen and thereby splits molecular oxygen to form water. To investigate the reaction mechanism of cytochrome c oxidase on the single vibrational level, we used...

journal_title：Biophysical journal

authors： Heitbrink D,Sigurdson H,Bolwien C,Brzezinski P,Heberle J

更新日期：2002-01-01 00:00:00

abstract：:We have studied the anion-dependent gating of roflamycoin ion channels using spectral analysis of noise in currents through multichannel planar lipid bilayers. We have found that in addition to low frequency current fluctuations that may be attributed to channel switching between open and closed conformations, roflamy...

journal_title：Biophysical journal

authors： Grigorjev PA,Bezrukov SM

更新日期：1994-12-01 00:00:00

abstract：:The fluorescent Mg(2+) indicator furaptra (mag-fura-2) was introduced into single ventricular myocytes by incubation with its acetoxy-methyl ester form. The ratio of furaptra's fluorescence intensity at 382 and 350 nm was used to estimate the apparent cytoplasmic [Mg(2+)] ([Mg(2+)](i)). In Ca(2+)-free extracellular co...

journal_title：Biophysical journal

authors： Tursun P,Tashiro M,Konishi M

更新日期：2005-03-01 00:00:00

abstract：:Mitochondria often reside in subcellular regions with high metabolic demands. We examined the mechanisms that can govern the relocation of mitochondria to these sites in respiratory neurons. Mitochondria were visualized using tetramethylrhodamineethylester, and their movements were analyzed by applying single-particle...

journal_title：Biophysical journal

authors： Mironov SL

更新日期：2007-04-15 00:00:00

abstract：:A new model is proposed to account for the apparent conductance changes of the sodium, or early, channel in nerve fiber membranes. In this model it is assumed that the channels are gated at the interior side of the membrane and are resistively limited at the exterior side by sodium selective barriers of high resistanc...

journal_title：Biophysical journal

authors： Hoyt RC,Strieb JD

更新日期：1971-11-01 00:00:00

abstract：:Using a method they developed, Stamatoff and Krimm (1976) have phased swelling data from nerve myelin. Although most phases agree with those I determined previously, there are a few differences. In this letter the two different phasings, theirs and my own, are used to compute the corresponding electron-density profile...

journal_title：Biophysical journal

authors： Blaurock AE

更新日期：1979-04-01 00:00:00

abstract：:Values of K, delta G(o), delta H(o), delta S(o) and delta C(po) for the binding reaction of small organic ligands forming 1:1 complexes with either alpha- or beta-cyclodextrin were obtained by titration calorimetry from 15 degrees C to 45 degrees C. A hydrogen bond or hydrophobic interaction was introduced by adding a...

journal_title：Biophysical journal

authors： Ross PD,Rekharsky MV

更新日期：1996-10-01 00:00:00

abstract：:In the photocycle of bacteriorhodopsin at pH 7, a proton is ejected to the extracellular medium during the protonation of Asp-85 upon formation of the M intermediate. The group that releases the ejected proton does not become reprotonated until the prephotolysis state is restored from the N and O intermediates. In con...

journal_title：Biophysical journal

authors： Morgan JE,Vakkasoglu AS,Lanyi JK,Lugtenburg J,Gennis RB,Maeda A

更新日期：2012-08-08 00:00:00

abstract：:The demembranated (skinned) muscle fiber preparation is widely used to investigate muscle contraction because the intracellular ionic conditions can be precisely controlled. However, plasma membrane removal results in a loss of osmotic regulation, causing abnormal hydration of the myofilament lattice and its proteins....

journal_title：Biophysical journal

authors： Tanner BC,Farman GP,Irving TC,Maughan DW,Palmer BM,Miller MS

更新日期：2012-09-19 00:00:00

abstract：:Charge movement currents (IQ) and calcium transients (delta[Ca2+]) were measured simultaneously in frog skeletal muscle fibers, voltage clamped in a double vaseline gap chamber, using Antipyrylazo III as the calcium indicator. The rate of release of calcium from the SR (Rrel) was calculated from the calcium transients...

journal_title：Biophysical journal

authors： Simon BJ,Hill DA

更新日期：1992-05-01 00:00:00