Geometry of normal mammalian platelets by quantitative microscopic studies.

abstract：:A nitroxide spin label attached to the C-terminus of the channel forming peptide alamethicin produces an enhancement of the nuclear spin-lattice relaxation rates of peptide protons as a result of both intermolecular and intramolecular magnetic dipole-dipole interactions. The intermolecular contribution provides eviden...

journal_title：Biophysical journal

authors： North CL,Franklin JC,Bryant RG,Cafiso DS

更新日期：1994-11-01 00:00:00

abstract：:In the photocycle of bacteriorhodopsin (bR) from Halobacterium salinarum mutant L93A, the O-intermediate accumulates and the cycling time is increased approximately 200 times. Nevertheless, under continuous illumination, the protein pumps protons at near wild-type rates. We excited the mutant L93A in purple membrane w...

journal_title：Biophysical journal

authors： Tóth-Boconádi R,Keszthelyi L,Stoeckenius W

更新日期：2003-06-01 00:00:00

abstract：:We study a Brownian dynamics simulation model of a biopolymeric shell deformed by axial forces exerted at opposing poles. The model exhibits two distinct, linear force-extension regimes, with the response to small tensions governed by linear elasticity and the response to large tensions governed by an effective spring...

journal_title：Biophysical journal

authors： Banigan EJ,Stephens AD,Marko JF

更新日期：2017-10-17 00:00:00

abstract：:Membranes made of dimyristoylphosphatidylcholine and cholesterol, one of the simplest paradigms for the study of liquid ordered-disordered phase separation, were investigated using a pulse-EPR spin-labeling method in which bimolecular collision of molecular oxygen with the nitroxide spin label is measured. This method...

journal_title：Biophysical journal

authors： Subczynski WK,Wisniewska A,Hyde JS,Kusumi A

更新日期：2007-03-01 00:00:00

abstract：:Resolving the dynamical interplay of proteins and lipids in the live-cell plasma membrane represents a central goal in current cell biology. Superresolution concepts have introduced a means of capturing spatial heterogeneity at a nanoscopic length scale. Similar concepts for detecting dynamical transitions (superresol...

journal_title：Biophysical journal

authors： Ruprecht V,Wieser S,Marguet D,Schütz GJ

更新日期：2011-06-08 00:00:00

abstract：:The antagonizing effect of high pressure against anesthesia is well known. With purified firefly luciferase, however,. Biophys. J. 60:1309-1314) reported that high pressure did not affect the initial flash intensity. Firefly luciferase emits a burst of light when the substrates luciferin and ATP are added in the prese...

journal_title：Biophysical journal

authors： Ueda I,Minami H,Matsuki H,Inoue T

更新日期：1999-01-01 00:00:00

abstract：:In this work we tested the hypothesis that transgenic sustained overexpression of IGF-1 prevents age-dependent decreases in charge movement and intracellular Ca(2+) in skeletal muscle fibers. To this end, short flexor digitorum brevis (FDB) muscle fibers from 5-7- and 21-24-month-old FVB (wild-type) and S1S2 (IGF-1 tr...

journal_title：Biophysical journal

authors： Wang ZM,Messi ML,Delbono O

更新日期：2002-03-01 00:00:00

abstract：:The conformation of mononucleosome complexes reconstituted with recombinant core histones on a 614-basepair-long DNA fragment containing the Xenopus borealis 5S rRNA nucleosome positioning sequence was studied by scanning/atomic force microscopy in the absence or presence of linker histone H1. Imaging without prior fi...

journal_title：Biophysical journal

authors： Kepert JF,Tóth KF,Caudron M,Mücke N,Langowski J,Rippe K

更新日期：2003-12-01 00:00:00

abstract：:The most probable source of force generation in muscle fibers in the rotation of the myosin head when bound to actin. This laboratory has demonstrated that ATP induces microsecond rotational motions of spin-labeled myosin heads bound to actin (Berger, C. L. E. C. Svensson, and D. D. Thomas. 1989. Proc. Natl. Acad. Sci...

journal_title：Biophysical journal

authors： Ostap EM,Thomas DD

更新日期：1991-06-01 00:00:00

abstract：:We analyzed the microstructure in the first-order laser diffraction line from both resting and tetanically contracting single twitch fibers from frog anterior tibial muscle to see if the distribution of sarcomere lengths is continuous or discrete. Measuring the distance between adjacent microstructural elements lying ...

journal_title：Biophysical journal

authors： Tameyasu T,Ishide N,Pollack GH

更新日期：1982-02-01 00:00:00

abstract：:Heme proteins react inhomogeneously with ligands at cryogenic temperatures and homogeneously at room temperature. We have identified and characterized a transition from inhomogeneous to homogeneous behavior at intermediate temperatures in the time dependence of CO binding to horse myoglobin. The turnover is attributed...

journal_title：Biophysical journal

authors： Agmon N,Doster W,Post F

更新日期：1994-05-01 00:00:00

abstract：:The selective permeability to monovalent metal cations, as well as the relationship between cation permeation and gating kinetics, was investigated for native tetrodotoxin-insensitive Na-channels in guinea pig ventricular myocytes using the whole-cell patch clamp technique. By the measurement of inward unidirectional ...

journal_title：Biophysical journal

authors： Kurata Y,Sato R,Hisatome I,Imanishi S

更新日期：1999-10-01 00:00:00

abstract：:We have developed new methodology for measuring intracellular pH (pHi) in cultured cell monolayers and epithelia by analyzing the emission spectra of the trapped fluorescent pH probe, 1,4-dihydroxyphthalonitrile (1,4-DHPN). This compound is unique since both its acid and base forms possess different fluorescence emiss...

journal_title：Biophysical journal

authors： Kurtz I,Balaban RS

更新日期：1985-09-01 00:00:00

abstract：:Activation of the photoreceptor G protein transducin (Gt) by opsin, the ligand-free form of rhodopsin, was measured using rod outer segment membranes with densities of opsin and Gt similar to those found in rod cells. When GTPgammaS was used as the activating nucleotide, opsin catalyzed transducin activation with an e...

journal_title：Biophysical journal

authors： Melia TJ Jr,Cowan CW,Angleson JK,Wensel TG

更新日期：1997-12-01 00:00:00

abstract：:Molecular dynamics simulations of wild-type bacteriorhodopsin (bR) and of its D85N, D85T, D212N, and Y57F mutants have been carried out to investigate possible differences in the photoproducts of these proteins. For each mutant, a series of 50 molecular dynamics simulations of the photoisomerization and subsequent rel...

journal_title：Biophysical journal

authors： Humphrey W,Bamberg E,Schulten K

更新日期：1997-03-01 00:00:00

abstract：:We extend the Saffman theory of membrane hydrodynamics to account for the correlated motion of membrane proteins, along with the effect of protein concentration on that correlation and on the response of the membrane to stresses. Expressions for the coupling diffusion coefficients of protein pairs and their concentrat...

journal_title：Biophysical journal

authors： Oppenheimer N,Diamant H

更新日期：2009-04-22 00:00:00

abstract：:It is shown that if x(t) is the solution of a second order differential equation, with real negative characteristic roots (not necessarily distinct), which exhibits an extremum at t = T, then T|x(T)|/|A| [UNK] 1/e where A is the area under the x(t) curve. This result is compared to a special case previously derived by...

journal_title：Biophysical journal

authors： HEARON JZ

更新日期：1961-09-01 00:00:00

abstract：:The physical mechanism underlying the voltage-dependent gating of K channels is usually addressed theoretically using molecular dynamics simulations. However, besides being computationally very expensive, this approach is presently unable to fully predict the behavior of fundamental variables of channel gating such as...

journal_title：Biophysical journal

authors： Catacuzzeno L,Franciolini F

更新日期：2019-11-19 00:00:00

abstract：:Low-power continuous-wave laser radiation is used to form a very stable microbubble at the end of a specially etched and metalized optical fiber probe. We demonstrate that the microbubble, which is firmly attached to the fiber probe, can be used to benignly trap and manipulate living swine sperm cells as well as human...

journal_title：Biophysical journal

authors： Diop M,Taylor R

更新日期：2006-05-15 00:00:00

abstract：:The effect of a transmembrane peptide on the domain structure of a two-component, two-phase lipid bilayer composed of dimyristoyl phosphatidylcholine (DMPC) and distearoyl phosphatidylcholine (DSPC) was examined by spin label electron spin resonance (ESR) spectroscopy. The peptide, pOmpA, is the hydrophobic, 25-residu...

journal_title：Biophysical journal

authors： Sankaram MB,Marsh D,Gierasch LM,Thompson TE

更新日期：1994-06-01 00:00:00

abstract：:The difference in the surface charge distribution between light-adapted and dark-adapted purple membranes was investigated with electric dichroism measurements from approximately pH 5 to pH 11. Purple membrane sheets in solution are oriented in a weak electric field by their permanent dipole moment, which is due to th...

journal_title：Biophysical journal

authors： Otomo J,Ohno K,Takeuchi Y,Ikegami A

更新日期：1986-08-01 00:00:00

abstract：:Human immunodeficiency virus-1 viral infectivity factor (Vif) is an intrinsically disordered protein responsible for the ubiquitination of the APOBEC3 (A3) antiviral proteins. Vif folds when it binds Cullin-RING E3 ligase 5 and the transcription cofactor CBF-β. A five-protein complex containing the substrate receptor ...

journal_title：Biophysical journal

authors： Ball KA,Chan LM,Stanley DJ,Tierney E,Thapa S,Ta HM,Burton L,Binning JM,Jacobson MP,Gross JD

更新日期：2019-04-23 00:00:00

abstract：:Circadian rhythms possess the ability to robustly entrain to the environmental cycles. This ability relies on the phase synchronization of circadian rhythm gene regulation to different environmental cues, of which light is the most obvious and important. The elucidation of the mechanism of circadian entrainment requir...

journal_title：Biophysical journal

authors： Gunawan R,Doyle FJ 3rd

更新日期：2006-09-15 00:00:00

abstract：:The main goal of this study is to investigate the role of mitochondrial [Ca(2+)], [Ca(2+)](m), in the possible up-regulation of the NADH production rate during increased workload. Such up-regulation is necessary to support increased flux through the electron transport chain and increased ATP synthesis rates. Intact ca...

journal_title：Biophysical journal

authors： Brandes R,Bers DM

更新日期：2002-08-01 00:00:00

abstract：:Equations are derived describing the dispersion of a permeable solute during Poiseuille flow in a capillary model. It is shown that for the normal range of physiological parameters such as capillary radius, capillary length, blood flow, permeability coefficients, and diffusion constants, the center of mass of a bolus ...

journal_title：Biophysical journal

authors： Pollock F,Blum JJ

更新日期：1966-01-01 00:00:00

abstract：:Pure Michaelis-Menten enzymes have been studied (i.e., enzymes with a hyperbolic (S, V) behavior in a well-stirred solution). When such enzymes are associated with a structure in vitro, even in the simplest conceivable form (immobilization in a homogeneous gel), they can produce enzymic or transport reactions with man...

journal_title：Biophysical journal

authors： Vincent JC,Thellier M

更新日期：1983-01-01 00:00:00

abstract：:We perform single-molecule spatial tracking measurements of a DNA repair protein, the C-terminal domain of Ada (C-Ada) from Escherichia coli, moving on DNA extended by flow. The trajectories of single proteins labeled with a fluorophore are constructed. We analyze single-protein dwell times on DNA for different flow r...

journal_title：Biophysical journal

authors： Lin Y,Zhao T,Jian X,Farooqui Z,Qu X,He C,Dinner AR,Scherer NF

更新日期：2009-03-04 00:00:00

abstract：:Two mechanisms for the interaction of alternating electrical fields with biological tissue are the development of heat, via i(2)R losses, and field-induced force effects, via differences in passive electrical properties. It has been shown that for continuous wave (CW) fields in media of physiologic electrical conducti...

journal_title：Biophysical journal

authors： Sher LD,Kresch E,Schwan HP

更新日期：1970-10-01 00:00:00

abstract：:Variation in the amplitude of miniature postsynaptic currents (mPSCs) generated by individual quanta of neurotransmitter is a major contributor to the variance of evoked synaptic responses. Here we explore the possible origins of this variability by developing a mathematical description of mPSC generation and consider...

journal_title：Biophysical journal

authors： Uteshev VV,Pennefather PS

更新日期：1996-09-01 00:00:00

abstract：:We investigate the interactions between lipid bilayers and amphiphilic peptides using a solvent-free coarse-grained simulation technique. In our model, each lipid is represented by one hydrophilic and three hydrophobic beads. The amphiphilic peptide is modeled as a hydrophobic-hydrophilic cylinder with hydrophilic cap...

journal_title：Biophysical journal

authors： Illya G,Deserno M

更新日期：2008-11-01 00:00:00