Soft trapping and manipulation of cells using a disposable nanoliter biochamber.

Abstract:

:Low-power continuous-wave laser radiation is used to form a very stable microbubble at the end of a specially etched and metalized optical fiber probe. We demonstrate that the microbubble, which is firmly attached to the fiber probe, can be used to benignly trap and manipulate living swine sperm cells as well as human embryonic kidney cells. The lifetime of the microbubble has been prolonged and the gaseous environment inside the bubble controlled using micropipette gas injection. The controlled fusion of two microbubbles is demonstrated as a means of transferring microparticles from one bubble to another. These experiments lay the foundation for the use of the microbubble as a mobile, nanoliter-volume disposable biochamber for cellular studies.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Diop M,Taylor R

doi

10.1529/biophysj.105.075614

subject

Has Abstract

pub_date

2006-05-15 00:00:00

pages

3813-22

issue

10

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(06)72561-9

journal_volume

90

pub_type

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