Soft trapping and manipulation of cells using a disposable nanoliter biochamber.


:Low-power continuous-wave laser radiation is used to form a very stable microbubble at the end of a specially etched and metalized optical fiber probe. We demonstrate that the microbubble, which is firmly attached to the fiber probe, can be used to benignly trap and manipulate living swine sperm cells as well as human embryonic kidney cells. The lifetime of the microbubble has been prolonged and the gaseous environment inside the bubble controlled using micropipette gas injection. The controlled fusion of two microbubbles is demonstrated as a means of transferring microparticles from one bubble to another. These experiments lay the foundation for the use of the microbubble as a mobile, nanoliter-volume disposable biochamber for cellular studies.


Biophys J


Biophysical journal


Diop M,Taylor R




Has Abstract


2006-05-15 00:00:00














  • 5-A Fourier map of gramicidin A phased by deuterium-hydrogen solvent difference neutron diffraction.

    abstract::Crystals of ion-free gramicidin A (P212121: a = 24.61, b = 32.28, c = 32.52) have been investigated using neutron diffraction. A difference analysis of crystals soaked in ethanol/H2O as opposed to ethanol-d6/D2O has led to single isomorphous replacement Fourier projections of the structure at 5-A resolution. The grami...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Koeppe RE 2nd,Schoenborn BP

    更新日期:1984-03-01 00:00:00

  • Backbone structure of the amantadine-blocked trans-membrane domain M2 proton channel from Influenza A virus.

    abstract::Amantadine is known to block the M2 proton channel of the Influenza A virus. Here, we present a structure of the M2 trans-membrane domain blocked with amantadine, built using orientational constraints obtained from solid-state NMR polarization-inversion-spin-exchange-at-the-magic-angle experiments. The data indicates ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Hu J,Asbury T,Achuthan S,Li C,Bertram R,Quine JR,Fu R,Cross TA

    更新日期:2007-06-15 00:00:00

  • Ratchetaxis in Channels: Entry Point and Local Asymmetry Set Cell Directions in Confinement.

    abstract::Cell motility is essential in a variety of biological phenomena ranging from early development to organ homeostasis and diseases. This phenomenon has mainly been studied and characterized on flat surfaces in vitro, whereas such conditions are rarely observed in vivo. Recently, cell motion in three-dimensional microfab...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Le Maout E,Lo Vecchio S,Kumar Korla P,Jinn-Chyuan Sheu J,Riveline D

    更新日期:2020-10-06 00:00:00

  • Low-frequency stimulation induces stable transitions in stereotypical activity in cortical networks.

    abstract::Reverberating spontaneous synchronized brain activity is believed to play an important role in neural information processing. Whether and how external stimuli can influence this spontaneous activity is poorly understood. Because periodic synchronized network activity is also prominent in in vitro neuronal cultures, we...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Vajda I,van Pelt J,Wolters P,Chiappalone M,Martinoia S,van Someren E,van Ooyen A

    更新日期:2008-06-01 00:00:00

  • Ligand detection and discrimination by spatial relocalization: A kinase-phosphatase segregation model of TCR activation.

    abstract::We develop a model of tyrosine phosphorylation and activation of the T-cell receptor (TCR) by localization to regions of close membrane-membrane proximity (close contact) that physically exclude tyrosine phosphatases such as CD45. Phosphatase exclusion generates regions of low phosphatase and high kinase activity and ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Burroughs NJ,Lazic Z,van der Merwe PA

    更新日期:2006-09-01 00:00:00

  • Dipole moment, enthalpy, and entropy changes of Hodgkin-Huxley type kinetic units.

    abstract::Dipole moment, enthalpy, and entropy changes were calculated for hypothetical structural units which control the opening and closing of ionic channels in axon membranes. The changes of these thermodynamic functions were calculated both for activation (transition to intermediate complex) and for the structural transfor...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Levitan E,Palti Y

    更新日期:1975-03-01 00:00:00

  • The effect of cross-bridge clustering and head-head competition on the mechanical response of skeletal muscle under equilibrium conditions.

    abstract::The effect of cross-bridge clustering and head-head competition on the mechanical response of skeletal muscle under equilibrium conditions is considered. For this purpose, the recent multiple site equilibrium cross-bridge model of Schoenberg (Schoenberg, M., 1985, Biophys. J., 48:467-475) is extended in accordance wit...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Tözeren A,Schoenberg M

    更新日期:1986-11-01 00:00:00

  • Measured effects of diacylglycerol on structural and elastic properties of phospholipid membranes.

    abstract::Diacylglycerol, a biological membrane second messenger, is a strong perturber of phospholipid planar bilayers. It converts multibilayers to the reverse hexagonal phase (HII), composed of highly curved monolayers. We have used x-ray diffraction and osmotic stress of the HII phase to measure structural dimensions, spont...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Leikin S,Kozlov MM,Fuller NL,Rand RP

    更新日期:1996-11-01 00:00:00

  • A microscopic multiphase diffusion model of viable epidermis permeability.

    abstract::A microscopic model of passive transverse mass transport of small solutes in the viable epidermal layer of human skin is formulated on the basis of a hexagonal array of cells (i.e., keratinocytes) bounded by 4-nm-thick, anisotropic lipid bilayers and separated by 1-μm layers of extracellular fluid. Gap junctions and t...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Nitsche JM,Kasting GB

    更新日期:2013-05-21 00:00:00

  • Effects of monovalent anions of the hofmeister series on DPPC lipid bilayers Part II: modeling the perpendicular and lateral equation-of-state.

    abstract::The effects of Hofmeister anions on the perpendicular and lateral equation-of-state (EOS) of the dipalmitoylphosphatidylcholine lamellar phase discussed in the companion article are here examined using appropriate free energy models for the intra- and interbilayer interactions. Minimizing the free energy with respect ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Leontidis E,Aroti A,Belloni L,Dubois M,Zemb T

    更新日期:2007-09-01 00:00:00

  • The Affinity of Sterols for Different Phospholipid Classes and Its Impact on Lateral Segregation.

    abstract::Cholesterol is an essential molecule in the membranes of mammalian cells. It is known to be distributed heterogeneously within the cells, between the bilayer leaflets, as well as between lateral domains within the bilayer. However, we do not know exactly how cholesterol is distributed and what forces drive this sortin...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Nyholm TKM,Jaikishan S,Engberg O,Hautala V,Slotte JP

    更新日期:2019-01-22 00:00:00

  • Membrane fusion and the lamellar-to-inverted-hexagonal phase transition in cardiolipin vesicle systems induced by divalent cations.

    abstract::The polymorphic phase behavior of bovine heart cardiolipin (CL) in the presence of different divalent cations and the kinetics of CL vesicle fusion induced by these cations have been investigated. (31)P-NMR measurements of equilibrium cation-CL complexes showed the lamellar-to-hexagonal (L(alpha)-H(II)) transition tem...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Ortiz A,Killian JA,Verkleij AJ,Wilschut J

    更新日期:1999-10-01 00:00:00

  • NaCl reflection coefficients in proximal tubule apical and basolateral membrane vesicles. Measurement by induced osmosis and solvent drag.

    abstract::Two independent methods, induced osmosis and solvent drag, were used to determine the reflection coefficients for NaCl (sigma NaCl) in brush border and basolateral membrane vesicles isolated from rabbit proximal tubule. In the induced osmosis method, vesicles loaded with sucrose were subjected to varying inward NaCl g...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Pearce D,Verkman AS

    更新日期:1989-06-01 00:00:00

  • Engineering aspects of enzymatic signal transduction: photoreceptors in the retina.

    abstract::Identifying the basic module of enzymatic amplification as an irreversible cycle of messenger activation/deactivation by a "push-pull" pair of opposing enzymes, we analyze it in terms of gain, bandwidth, noise, and power consumption. The enzymatic signal transduction cascade is viewed as an information channel, the de...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Detwiler PB,Ramanathan S,Sengupta A,Shraiman BI

    更新日期:2000-12-01 00:00:00

  • Topography of Cells Revealed by Variable-Angle Total Internal Reflection Fluorescence Microscopy.

    abstract::We propose an improved version of variable-angle total internal reflection fluorescence microscopy (vaTIRFM) adapted to modern TIRF setup. This technique involves the recording of a stack of TIRF images, by gradually increasing the incident angle of the light beam on the sample. A comprehensive theory was developed to...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Cardoso Dos Santos M,Déturche R,Vézy C,Jaffiol R

    更新日期:2016-09-20 00:00:00

  • Fluorescence dequenching kinetics of single cell-cell fusion complexes.

    abstract::In an earlier paper which models the cell-cell (or virus-cell) fusion complex as two partial spherical vesicles joined at a narrow neck (Rubin, R. J., and Yi-der Chen. 1990. Biophys. J. 58:1157-1167), the redistribution by diffusion of lipid-like molecules through the neck between the two fused cell surfaces was studi...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Chen YD,Rubin RJ,Szabo A

    更新日期:1993-07-01 00:00:00

  • Bacteriorhodopsin wildtype and variant aspartate-96 --> aspargine as reversible holographic media.

    abstract::Air dried films of purple membranes (PM) from Halobacterium halobium containing the photochromic protein bacteriorhodopsin (BR) were prepared and the BR-photocycle of this material analyzed. The absorption maxima of the initial state B (lambda(max) = 570 nm) and the photochemical intermediate M (lambda(max) = 412 nm),...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Hampp N,Bräuchle C,Oesterhelt D

    更新日期:1990-07-01 00:00:00

  • Quantitatively imaging chromosomes by correlated cryo-fluorescence and soft x-ray tomographies.

    abstract::Soft x-ray tomography (SXT) is increasingly being recognized as a valuable method for visualizing and quantifying the ultrastructure of cryopreserved cells. Here, we describe the combination of SXT with cryogenic confocal fluorescence tomography (CFT). This correlative approach allows the incorporation of molecular lo...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Smith EA,McDermott G,Do M,Leung K,Panning B,Le Gros MA,Larabell CA

    更新日期:2014-10-21 00:00:00

  • Effect of pore structure on energy barriers and applied voltage profiles. II. Unsymmetrical channels.

    abstract::This paper examines "realistic" pores, i.e., ones that are neither symmetric nor of uniform diameter. Methods are described that permit estimation of the image potential for an ion in an aqueous pore spanning a lipid membrane and for the electric field produced in such a pore when a transmembrane potential is applied....

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Jordan PC

    更新日期:1984-06-01 00:00:00

  • Structure and dynamics of Escherichia coli chemosensory receptors. Engineered sulfhydryl studies.

    abstract::Cysteine residues introduced by site-directed mutagenesis have been used to probe the conformation and dynamics of two receptors in the E. coli chemotaxis pathway. (a) Thermal motions of the polypeptide backbone were investigated in the periplasmic D-galactose and D-glucose receptor, a globular protein of known struct...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Careaga CL,Falke JJ

    更新日期:1992-04-01 00:00:00

  • ATP-sensitive potassium channel and bursting in the pancreatic beta cell. A theoretical study.

    abstract::Based on the existence of ATP-sensitive potassium channels in the plasma membrane of pancreatic beta cells, we develop a quantitative explanation of the electrical activity observed in pancreatic islets. The proposed mechanism involves the voltage-dependent inward calcium and outward potassium currents described by Ro...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Keizer J,Magnus G

    更新日期:1989-08-01 00:00:00

  • Structural Modeling of GR Interactions with the SWI/SNF Chromatin Remodeling Complex and C/EBP.

    abstract::The glucocorticoid receptor (GR) is a steroid-hormone-activated transcription factor that modulates gene expression. Transcriptional regulation by the GR requires dynamic receptor binding to specific target sites located across the genome. This binding remodels the chromatin structure to allow interaction with other t...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Muratcioglu S,Presman DM,Pooley JR,Grøntved L,Hager GL,Nussinov R,Keskin O,Gursoy A

    更新日期:2015-09-15 00:00:00

  • Coding design of positional information for robust morphogenesis.

    abstract::Robust positioning of cells in a tissue against unavoidable noises is important for achieving normal and reproducible morphogenesis. The position in a tissue is represented by morphogen concentrations, and cells read them to recognize their spatial coordinates. From the engineering viewpoint, these positioning process...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Morishita Y,Iwasa Y

    更新日期:2011-11-16 00:00:00

  • Dendritic signals command firing dynamics in a mathematical model of cerebellar Purkinje cells.

    abstract::Dendrites of cerebellar Purkinje cells (PCs) respond to brief excitations from parallel fibers with lasting plateau depolarizations. It is unknown whether these plateaus are local events that boost the synaptic signals or they propagate to the soma and directly take part in setting the cell firing dynamics. To address...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Genet S,Sabarly L,Guigon E,Berry H,Delord B

    更新日期:2010-07-21 00:00:00

  • Electron-nuclear double resonance and hyperfine sublevel correlation spectroscopic studies of flavodoxin mutants from Anabaena sp. PCC 7119.

    abstract::The influence of the amino acid residues surrounding the flavin ring in the flavodoxin of the cyanobacterium Anabaena PCC 7119 on the electron spin density distribution of the flavin semiquinone was examined in mutants of the key residues Trp(57) and Tyr(94) at the FMN binding site. Neutral semiquinone radicals of the...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Medina M,Lostao A,Sancho J,Gómez-Moreno C,Cammack R,Alonso PJ,Martínez JI

    更新日期:1999-09-01 00:00:00

  • Photovoltage kinetics of the acid-blue and acid-purple forms of bacteriorhodopsin: evidence for no net charge transfer.

    abstract::Time-resolved photovoltage measurements were performed with the acid-blue (bR605A) and acid-purple (bR565A) forms of bacteriorhodopsin (bR) in the time range from 25 ns to 100 s. The bR605A and bR565A pigments were formed by titration with H2SO4 in the absence and presence of 150 mM KCI, respectively. Qualitatively th...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Moltke S,Heyn MP

    更新日期:1995-11-01 00:00:00

  • Differential stability of beta-sheets and alpha-helices in beta-lactamase: a high temperature molecular dynamics study of unfolding intermediates.

    abstract::beta-Lactamase, which catalyzes beta-lactam antibiotics, is prototypical of large alpha/beta proteins with a scaffolding formed by strong noncovalent interactions. Experimentally, the enzyme is well characterized, and intermediates that are slightly less compact and having nearly the same content of secondary structur...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Vijayakumar S,Vishveshwara S,Ravishanker G,Beveridge DL

    更新日期:1993-12-01 00:00:00

  • Systematic size study of an insect antifreeze protein and its interaction with ice.

    abstract::Because of their remarkable ability to depress the freezing point of aqueous solutions, antifreeze proteins (AFPs) play a critical role in helping many organisms survive subzero temperatures. The beta-helical insect AFP structures solved to date, consisting of multiple repeating circular loops or coils, are perhaps th...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Liu K,Jia Z,Chen G,Tung C,Liu R

    更新日期:2005-02-01 00:00:00

  • Protein stiffening and entropic stabilization in the subdenaturing limit of guanidine hydrochloride.

    abstract::Subdenaturing concentrations of guanidine hydrochloride (GdnHCl) stabilize proteins. For ferrocytochrome c the stabilization is detected at subglobal level with no measured change in global stability. These deductions are made by comparing observed rates of thermally driven ferrocytochrome cHCO reactions with global u...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Kumar R,Prabhu NP,Yadaiah M,Bhuyan AK

    更新日期:2004-10-01 00:00:00

  • Solid-state NMR studies of a diverged microsomal amino-proximate delta12 desaturase peptide reveal causes of stability in bilayer: tyrosine anchoring and arginine snorkeling.

    abstract::This study reports the solid-state NMR spectroscopic characterization of the amino-proximate transmembrane domain (TM-A) of a diverged microsomal delta12-desaturase (CREP-1) in a phospholipid bilayer. A series of TM-A peptides were synthesized with 2H-labeled side chains (Ala-53, -56, and -63, Leu-62, Val-50), and the...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Gibbons WJ Jr,Karp ES,Cellar NA,Minto RE,Lorigan GA

    更新日期:2006-02-15 00:00:00