Abstract:
:Low-power continuous-wave laser radiation is used to form a very stable microbubble at the end of a specially etched and metalized optical fiber probe. We demonstrate that the microbubble, which is firmly attached to the fiber probe, can be used to benignly trap and manipulate living swine sperm cells as well as human embryonic kidney cells. The lifetime of the microbubble has been prolonged and the gaseous environment inside the bubble controlled using micropipette gas injection. The controlled fusion of two microbubbles is demonstrated as a means of transferring microparticles from one bubble to another. These experiments lay the foundation for the use of the microbubble as a mobile, nanoliter-volume disposable biochamber for cellular studies.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Diop M,Taylor Rdoi
10.1529/biophysj.105.075614subject
Has Abstractpub_date
2006-05-15 00:00:00pages
3813-22issue
10eissn
0006-3495issn
1542-0086pii
S0006-3495(06)72561-9journal_volume
90pub_type
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