Structural dynamics of ligand diffusion in the protein matrix: A study on a new myoglobin mutant Y(B10) Q(E7) R(E10).

abstract：:We have analyzed repellent signal processing in Escherichia coli by flash photorelease of leucine from photolabile precursors. We found that 1). response amplitudes of free-swimming cell populations increased with leucine jump concentration, with an apparent Hill coefficient of 1.3 and a half-maximal dose of 14.4 micr...

journal_title：Biophysical journal

authors： Khan S,Jain S,Reid GP,Trentham DR

更新日期：2004-06-01 00:00:00

abstract：:Red blood cells aggregate face-to-face to form long, cylindrical, straight chains and sometimes branched structures called rouleaux. Here we extend a kinetic model developed by R. W. Samsel and A. S. Perelson (1982, Biophys. J. 37:493-514) to include both the formation and dissociation of rouleaux. We examine thermody...

journal_title：Biophysical journal

authors： Samsel RW,Perelson AS

更新日期：1984-04-01 00:00:00

abstract：:We study the folding thermodynamics and kinetics of the Pin1 WW domain, a three-stranded beta-sheet protein, by using all-atom (except nonpolar hydrogens) discontinuous molecular dynamics simulations at various temperatures with a Gō model. The protein exhibits a two-state folding kinetics near the folding transition ...

journal_title：Biophysical journal

authors： Luo Z,Ding J,Zhou Y

更新日期：2007-09-15 00:00:00

abstract：:Unlike most transmembrane proteins, phospholipids can migrate from one leaflet of the membrane to the other. Because this spontaneous lipid translocation (flip-flop) tends to be very slow, cells facilitate the process with enzymes that catalyze the transmembrane movement and thereby regulate the transbilayer lipid dis...

journal_title：Biophysical journal

authors： Doktorova M,Heberle FA,Marquardt D,Rusinova R,Sanford RL,Peyear TA,Katsaras J,Feigenson GW,Weinstein H,Andersen OS

更新日期：2019-03-05 00:00:00

abstract：:Intercellular channels formed of members of the gene family of connexins (Cxs) vary from being substantially cation selective to being anion selective. We took advantage of the ability of Cx46 to function as an unopposed hemichannel to examine the basis of Cx charge selectivity. Previously we showed Cx46 hemichannels ...

journal_title：Biophysical journal

authors： Trexler EB,Bukauskas FF,Kronengold J,Bargiello TA,Verselis VK

更新日期：2000-12-01 00:00:00

abstract：:Many animals regulate power generation for locomotion by varying the number of muscle fibers used for movement. However, insects with asynchronous flight muscles may regulate the power required for flight by varying the calcium concentration ([Ca(2+)]). In vivo myoplasmic calcium levels in Drosophila flight muscle hav...

journal_title：Biophysical journal

authors： Wang Q,Zhao C,Swank DM

更新日期：2011-11-02 00:00:00

abstract：:Proteins are denatured in aqueous urea solution. The nature of the molecular driving forces has received substantial attention in the past, whereas the question how urea acts at different phases of unfolding is not yet well understood at the atomic level. In particular, it is unclear whether urea actively attacks fold...

journal_title：Biophysical journal

authors： Stumpe MC,Grubmüller H

更新日期：2009-05-06 00:00:00

abstract：:A recently developed technique for in vivo determination of the electrical resistance of vascular endothelium in microvessels was applied to the vessels in a thin frog muscle, m. cutaneus pectoris. The technique consists of injection of current via a glass micropipette into a capillary and measurement of the resulting...

journal_title：Biophysical journal

authors： Olesen SP,Crone C

更新日期：1983-04-01 00:00:00

abstract：:Outside-out patches of enzymatically dissociated adult and denervated mouse muscle fibers were superfused repetitively by pulses of acetylcholine (ACh) containing solution. Up to 300 channels opened simultaneously 300 microseconds after the beginning of a 1,000 microM ACh pulse corresponding to a peak current i of alm...

journal_title：Biophysical journal

authors： Franke C,Hatt H,Parnas H,Dudel J

更新日期：1991-11-01 00:00:00

abstract：:A potassium-selective, chemically excitable channel, whose characteristics cannot be accurately described by constant-field theory, is studied by a new approach based on diffusion theory but with no need for the classical assumptions of constant field, homogeneous membrane, and equal phase-boundary potentials at both ...

journal_title：Biophysical journal

authors： Schwartz TL,Kado RT

更新日期：1977-06-01 00:00:00

abstract：:To assay cell cycle progression in synchronized culture of yeast we have applied dielectric spectroscopy to its real-time monitoring. The dielectric monitoring is based on the electromagnetic induction method, regarded as a nonelectrode method, which has resolved the problems encountered in measurements with metal ele...

journal_title：Biophysical journal

authors： Asami K,Gheorghiu E,Yonezawa T

更新日期：1999-06-01 00:00:00

abstract：:To elucidate the mechanisms of cooperativity of cytochrome P450eryF an SH-reactive fluorescent probe was introduced close to the substrate-binding site. Cys-154, the only accessible cysteine, was eliminated by site-directed mutagenesis, and a novel cysteine was substituted for Ser-93 in the B'/C loop. S93C, C154A, C15...

journal_title：Biophysical journal

authors： Davydov DR,Botchkareva AE,Davydova NE,Halpert JR

更新日期：2005-07-01 00:00:00

abstract：:KL(4) is a 21-residue functional peptide mimic of lung surfactant protein B, an essential protein for lowering surface tension in the alveoli. Its ability to modify lipid properties and restore lung compliance was investigated with circular dichroism, differential scanning calorimetry, and solid-state NMR spectroscopy...

journal_title：Biophysical journal

authors： Antharam VC,Elliott DW,Mills FD,Farver RS,Sternin E,Long JR

更新日期：2009-05-20 00:00:00

abstract：:Quantitative analysis of DNA microarray data is complicated by uncertainties inherent to the experimental setup. Using computer simulations and real-time experimental results, we have previously demonstrated effects of multiplex reactions on a single sensing zone of an array, which may be a leading factor in erroneous...

journal_title：Biophysical journal

authors： Bishop J,Chagovetz AM,Blair S

更新日期：2008-03-01 00:00:00

abstract：:Ca2+ transport through mitochondrial Ca2+ uniporter is the primary Ca2+ uptake mechanism in respiring mitochondria. Thus, the uniporter plays a key role in regulating mitochondrial Ca2+. Despite the importance of mitochondrial Ca2+ to metabolic regulation and mitochondrial function, and to cell physiology and pathophy...

journal_title：Biophysical journal

authors： Dash RK,Qi F,Beard DA

更新日期：2009-02-18 00:00:00

abstract：:A new method for measuring the mechanical forces exerted by cells on the substratum and through the substratum to act on other cells is described. This method depends upon the growth of cells on a photoelastic substratum, polydimethylsiloxane coated with a near monolayer of fibronectin. Changes in the forces applied b...

journal_title：Biophysical journal

authors： Curtis A,Sokolikova-Csaderova L,Aitchison G

更新日期：2007-03-15 00:00:00

abstract：:In an open circuit there can be no net cation flux through membranes containing only cation-selective channels, because electroneutrality must be maintained. If the channels are so narrow that water and cations cannot pass by each other, then the net water flux through those "single-file" channels that contain a catio...

journal_title：Biophysical journal

authors： Dani JA,Levitt DG

更新日期：1981-08-01 00:00:00

abstract：:General anesthesia is a relatively safe medical procedure, which for nearly 170 years has allowed life saving surgical interventions in animals and people. However, the molecular mechanism of general anesthesia continues to be a matter of importance and debate. A favored hypothesis proposes that general anesthesia res...

journal_title：Biophysical journal

authors： Covarrubias M,Barber AF,Carnevale V,Treptow W,Eckenhoff RG

更新日期：2015-11-17 00:00:00

abstract：:The development of scanning probe techniques has made it possible to examine protein-protein interactions at the level of individual molecular pairs. A calibrated optical tweezers, along with immunoglobulin G (IgG)-coated polystyrene microspheres, has been used to detect individual surface-linked Staphylococcus protei...

journal_title：Biophysical journal

authors： Stout AL

更新日期：2001-06-01 00:00:00

abstract：:Phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) are the main lipid components of the inner bacterial membrane. A computer model for such a membrane was built of palmitoyloleoyl PE (POPE) and palmitoyloleoyl PG (POPG) in the proportion 3:1, and sodium ions (Na+) to neutralize the net negative charge on each...

journal_title：Biophysical journal

authors： Murzyn K,Róg T,Pasenkiewicz-Gierula M

更新日期：2005-02-01 00:00:00

abstract：:The rotation of human red blood cells (RBC) as they flow in the shear field established by a Coulter type orifice is modeled. This model, based on hydrodynamics of ellipsoid rotation in laminar creeping flow, is used to calculate the probability of the cells entering the orifice with a specific orientation. The electr...

journal_title：Biophysical journal

authors： Breitmeyer MO,Lightfoot EN,Dennis WH

更新日期：1971-02-01 00:00:00

abstract：:Molecular dynamics calculations demonstrated the conformational change in the prion protein due to Ala(117)-->Val mutation, which is related to Gerstmann-Sträussler-Sheinker disease, one of the familial prion diseases. Three kinds of model structures of human and mouse prion proteins were examined: (model 1) nuclear m...

journal_title：Biophysical journal

authors： Okimoto N,Yamanaka K,Suenaga A,Hata M,Hoshino T

更新日期：2002-05-01 00:00:00

abstract：:An analysis of four 1-ns molecular dynamics trajectories for two different 15-bp oligonucleotides is presented. Our aim is to show which groups of atoms can be treated as rigid bodies within a bead representation of DNA, independently of the base sequence and for any conformations belonging to the A/B family. Five mod...

journal_title：Biophysical journal

authors： Bruant N,Flatters D,Lavery R,Genest D

更新日期：1999-11-01 00:00:00

abstract：:Pyranine (8-hydroxy pyrene-1,3,6-trisulfonate) is a commonly used photoacid that discharges a proton when excited to its first electronic singlet state. Follow-up of its dissociation kinetics reveals the physicochemical properties of its most immediate environment. At vanishing ionic strength the dye adsorbs to the Es...

journal_title：Biophysical journal

authors： Nachliel E,Pollak N,Huppert D,Gutman M

更新日期：2001-03-01 00:00:00

abstract：:Proteins fold and function inside cells that are crowded with macromolecules. Here, we address the role of the resulting excluded volume effects by in vitro spectroscopic studies of Pseudomonas aeruginosa apoazurin stability (thermal and chemical perturbations) and folding kinetics (chemical perturbation) as a functio...

journal_title：Biophysical journal

authors： Christiansen A,Wittung-Stafshede P

更新日期：2013-10-01 00:00:00

abstract：:We have used resonance Raman spectroscopy to probe the heme environment of a recently discovered NOS from the pathogenic bacterium Staphylococcus aureus, named SANOS. We detect two forms of the CO complex in the absence of L-arginine, with nu(Fe-CO) at 482 and 497 cm(-1) and nu(C-O) at 1949 and 1930 cm(-1), respective...

journal_title：Biophysical journal

authors： Chartier FJ,Couture M

更新日期：2004-09-01 00:00:00

abstract：:The interaction of the nonionic surfactant dodecylmaltoside (DM) with phosphatidylcholine liposomes was investigated. Permeability alterations were detected as a change in 5(6)-carboxyfluorescein released from the interior of vesicles and bilayer solubilization as a decrease in the static light scattered by liposome s...

journal_title：Biophysical journal

authors： de la Maza A,Parra JL

更新日期：1997-04-01 00:00:00

abstract：:4',6-diamidino-2-phenylindole (DAPI), netropsin, and pentamidine are minor groove binders that have terminal -C(NH2)2+ groups. The hydration changes that accompany their binding to the minor groove of the (AATT)2 sequence have been studied using the osmotic stress technique with fluorescence spectroscopy. The affinity...

journal_title：Biophysical journal

authors： Degtyareva NN,Wallace BD,Bryant AR,Loo KM,Petty JT

更新日期：2007-02-01 00:00:00

abstract：:2H-nuclear magnetic resonance (NMR) has been used to study the dynamics of amino acid residues in bacteriorhodopsin with results that depend on the method of sample preparation. We show here that in [2H]-leucine-labeled samples the intensity of the isotropic signal varies according to the degree of residual contaminat...

journal_title：Biophysical journal

authors： Herzfeld J,Mulliken CM,Siminovitch DJ,Griffin RG

更新日期：1987-11-01 00:00:00

abstract：:The interactions between vitamin-A and beta-lactoglobulin have been investigated. We have found that two different complexes can be formed: one involving vitamin-A, and one involving a derivative of vitamin-A that most probably has a retro-beta-ionylidene structure. Room temperature absorption spectra for these comple...

journal_title：Biophysical journal

authors： Hemley R,Kohler BE,Siviski P

更新日期：1979-12-01 00:00:00