From atomic to mesoscopic descriptions of the internal dynamics of DNA.

abstract：:The effect of salt and pH titration on the selectivity of spin-labeled analogues of phosphatidic acid, phosphatidylserine, phosphatidylcholine, and stearic acid for the nicotinic acetylcholine receptor (nAcChoR) reconstituted into dioleoylphosphatidylcholine was examined at 0 degrees C using electron spin resonance sp...

journal_title：Biophysical journal

authors： Raines DE,Miller KW

更新日期：1993-03-01 00:00:00

abstract：:The current rapid expansion of biological knowledge offers a great opportunity to rationally engineer biological systems that respond to signals such as light and chemical inducers by producing specific proteins. Turning on and off the production of proteins on demand holds great promise for creating significant biote...

journal_title：Biophysical journal

authors： Tuttle LM,Salis H,Tomshine J,Kaznessis YN

更新日期：2005-12-01 00:00:00

abstract：:We study a Brownian dynamics simulation model of a biopolymeric shell deformed by axial forces exerted at opposing poles. The model exhibits two distinct, linear force-extension regimes, with the response to small tensions governed by linear elasticity and the response to large tensions governed by an effective spring...

journal_title：Biophysical journal

authors： Banigan EJ,Stephens AD,Marko JF

更新日期：2017-10-17 00:00:00

abstract：:Autoregulatory feedback loops are one of the most common network motifs. A wide variety of stochastic models have been constructed to understand how the fluctuations in protein numbers in these loops are influenced by the kinetic parameters of the main biochemical steps. These models differ according to 1) which subce...

journal_title：Biophysical journal

authors： Holehouse J,Cao Z,Grima R

更新日期：2020-04-07 00:00:00

abstract：:Stabilization of the lipid bilayer membrane in red blood cells by its association with an underlying membrane-associated cytoskeleton has long been recognized as critical for proper red blood cell function. One of the principal connections between skeleton and bilayer is via linkages between band 3, the integral membr...

journal_title：Biophysical journal

authors： Butler J,Mohandas N,Waugh RE

更新日期：2008-08-01 00:00:00

abstract：:A block normal mode (BNM) algorithm, originally proposed by Tama et al., (Proteins Struct. Func. Genet. 41:1-7, 2000) was implemented into the simulation program CHARMM. The BNM approach projects the hessian matrix into local translation/rotation basis vectors and, therefore, dramatically reduces the size of the matri...

journal_title：Biophysical journal

authors： Li G,Cui Q

更新日期：2002-11-01 00:00:00

abstract：:We report the deposition of lipid monolayers and bilayers on polyacrylamide films deposited by radical chain reaction onto solid substrates in aqueous solutions. Polymer films of various degrees of monomer density and cross-linking are prepared. Lateral diffusion and fluorescent probe permeation measurements yield ins...

journal_title：Biophysical journal

authors： Kühner M,Tampé R,Sackmann E

更新日期：1994-07-01 00:00:00

abstract：:We report here a method for the determination of the pKa of histidine in complex or heterogeneous systems amenable to neither solid-state nor solution NMR spectroscopy. Careful synthesis of a fluorenylmethyloxycarbonyl- and trityl-protected, C2-deuterated histidine produces a vibrational-probe-equipped amino acid that...

journal_title：Biophysical journal

authors： Braun GA,Pogostin BH,Pucetaite M,Londergan CH,Åkerfeldt KS

更新日期：2020-11-03 00:00:00

abstract：:We have developed a saturation transfer EPR (ST-EPR) method to measure selectively the rotational dynamics of those lipids that are motionally restricted by integral membrane proteins and have applied this methodology to measure lipid-protein interactions in native sarcoplasmic reticulum (SR) membranes. This analysis ...

journal_title：Biophysical journal

authors： Squier TC,Thomas DD

更新日期：1989-10-01 00:00:00

abstract：:The x-ray structure analysis of photosystem I (PS I) crystals at 4-A resolution (Schubert et al., 1997, J. Mol. Biol. 272:741-769) has revealed the distances between the three iron-sulfur clusters, labeled F(X), F(1), and F(2), which function on the acceptor side of PS I. There is a general consensus concerning the as...

journal_title：Biophysical journal

authors： Shinkarev VP,Vassiliev IR,Golbeck JH

更新日期：2000-01-01 00:00:00

abstract：:Many proteins contain flexible structures such as loops and hinged domains. A simple root mean square deviation (RMSD) alignment of two different conformations of the same protein can be skewed by the difference between the mobile regions. To overcome this problem, we have developed a novel method to overlay two prote...

journal_title：Biophysical journal

authors： Damm KL,Carlson HA

更新日期：2006-06-15 00:00:00

abstract：:We investigated the origins of greater clot rigidity associated with FXIIIa-dependent cross-linking. Fibrin clots were examined in which cross-linking was controlled through the use of two inhibitors: a highly specific active-center-directed synthetic inhibitor of FXIIIa, 1,3-dimethyl-4,5-diphenyl-2[2(oxopropyl)thio]i...

journal_title：Biophysical journal

authors： Ryan EA,Mockros LF,Stern AM,Lorand L

更新日期：1999-11-01 00:00:00

abstract：:The mechanosensitive channel of small conductance (MscS) is a bacterial mechanosensitive channel that opens in response to rapid hypoosmotic stress. Since MscS can be opened solely by membrane stretch without help from any accessory protein, the lipid-protein interface must play a crucial role in sensing membrane tens...

journal_title：Biophysical journal

authors： Nomura T,Sokabe M,Yoshimura K

更新日期：2006-10-15 00:00:00

abstract：:We present an analysis of physical chemical constraints on the accuracy of DNA micro-arrays under equilibrium and nonequilibrium conditions. At the beginning of the article we describe an algorithm for choosing a probe set with high specificity for targeted genes under equilibrium conditions. The algorithm as well as ...

journal_title：Biophysical journal

authors： Bhanot G,Louzoun Y,Zhu J,DeLisi C

更新日期：2003-01-01 00:00:00

abstract：:The increasingly widespread use of parametric mathematical models to describe biological systems means that the ability to infer model parameters is of great importance. In this study, we consider parameter inferability in nonlinear ordinary differential equation models that undergo a bifurcation, focusing on a simple...

journal_title：Biophysical journal

authors： Kirk PD,Toni T,Stumpf MP

更新日期：2008-07-01 00:00:00

abstract：:Flow dialysis has found widespread use in determining the dissociation constant (KD) of a protein-ligand interaction or the amount of available binding sites (E0). This method has the potency to measure both these parameters in a single experiment and in this article a method to measure simultaneously the KD and E0 is...

journal_title：Biophysical journal

authors： Veldhuis G,Vos EP,Broos J,Poolman B,Scheek RM

更新日期：2004-04-01 00:00:00

abstract：:The proposition is made that the red cell membrane is a two-dimensional, incompressible material and a general stress-strain law is developed for finite deformations. In the linear form, the character of such a material is analogous to a two-dimensional Mooney material (e.g., rubber), indicating that the molecular str...

journal_title：Biophysical journal

authors： Evans EA

更新日期：1973-09-01 00:00:00

abstract：:The M2 protein from the influenza A virus forms a proton channel in the virion that is essential for infection. This tetrameric protein appears to form a four-helix bundle spanning the viral membrane. Here the solid-state NMR method, 2D polarization inversion spin exchange at magic angle (PISEMA), has been used to obt...

journal_title：Biophysical journal

authors： Song Z,Kovacs FA,Wang J,Denny JK,Shekar SC,Quine JR,Cross TA

更新日期：2000-08-01 00:00:00

abstract：:The Kir3.1/Kir3.4 channel is an inward rectifier, agonist-activated K(+) channel. The location of the binding site within the channel pore that coordinates polyamines (and is thus responsible for inward rectification) and the location of the gate that opens the channel in response to agonist activation is unclear. In ...

journal_title：Biophysical journal

authors： Makary SM,Claydon TW,Dibb KM,Boyett MR

更新日期：2006-06-01 00:00:00

abstract：:A mathematical model is formulated for the development of a population of cells in which the individual members may grow and divide or die. A given cell is characterized by its age and volume, and these parameters are assumed to determine the rate of volume growth and the probability per unit time of division or death...

journal_title：Biophysical journal

authors： Bell GI,Anderson EC

更新日期：1967-07-01 00:00:00

abstract：:Fibril deposit formation of amyloid beta-protein (Abeta) in the brain is a hallmark of Alzheimer's disease (AD). Increasing evidence suggests that toxicity is linked to diffusible Abeta oligomers, which have been found in soluble brain extracts of AD patients, rather than to insoluble fibers. Here we report a study of...

journal_title：Biophysical journal

authors： Picone P,Carrotta R,Montana G,Nobile MR,San Biagio PL,Di Carlo M

更新日期：2009-05-20 00:00:00

abstract：:Dendrites of cerebellar Purkinje cells (PCs) respond to brief excitations from parallel fibers with lasting plateau depolarizations. It is unknown whether these plateaus are local events that boost the synaptic signals or they propagate to the soma and directly take part in setting the cell firing dynamics. To address...

journal_title：Biophysical journal

authors： Genet S,Sabarly L,Guigon E,Berry H,Delord B

更新日期：2010-07-21 00:00:00

abstract：:In response to external stimuli, cells modulate their adhesive state by regulating the number and intrinsic affinity of receptor/ligand bonds. A number of studies have shown that cell adhesion is dramatically reduced at room or lower temperatures as compared with physiological temperature. However, the underlying mech...

journal_title：Biophysical journal

authors： Rico F,Chu C,Abdulreda MH,Qin Y,Moy VT

更新日期：2010-09-08 00:00:00

abstract：:Gap junctions formed of connexin46 (Cx46) and connexin50 (Cx50) in lens fiber cells are crucial for maintaining lens transparency. We determined the functional properties of homotypic Cx46, heterotypic Cx46/Cx50, and heteromeric Cx46/Cx50 channels in a communication-deficient neuroblastoma (N2A) cell line, using dual ...

journal_title：Biophysical journal

authors： Hopperstad MG,Srinivas M,Spray DC

更新日期：2000-10-01 00:00:00

abstract：:The interactions between vitamin-A and beta-lactoglobulin have been investigated. We have found that two different complexes can be formed: one involving vitamin-A, and one involving a derivative of vitamin-A that most probably has a retro-beta-ionylidene structure. Room temperature absorption spectra for these comple...

journal_title：Biophysical journal

authors： Hemley R,Kohler BE,Siviski P

更新日期：1979-12-01 00:00:00

abstract：:Photodynamic therapy (PDT) is generally based on the generation of highly reactive singlet oxygen ((1)O(2)) through interactions of photosensitizer, light, and oxygen ((3)O(2)). These three components are highly interdependent and dynamic, resulting in variable temporal and spatial (1)O(2) dose deposition. Robust dosi...

journal_title：Biophysical journal

authors： Jarvi MT,Patterson MS,Wilson BC

更新日期：2012-02-08 00:00:00

abstract：:Electrically excitable channels were expressed in Chinese hamster ovary cells using a vaccinia virus vector system. In cells expressing rat brain IIA Na+ channels only, brief pulses (< 1 ms) of depolarizing current resulted in action potentials with a prolonged (0.5-3 s) depolarizing plateau; this plateau was caused b...

journal_title：Biophysical journal

authors： Hsu H,Huang E,Yang XC,Karschin A,Labarca C,Figl A,Ho B,Davidson N,Lester HA

更新日期：1993-09-01 00:00:00

abstract：:We have used anomalous small-angle x-ray scattering as a structural probe for solutions of rabbit parvalbumin labeled with terbium. This technique makes use of the large changes in the terbium scattering factor that occur when the x-ray energy is tuned around an L3 absorption edge of this heavy-atom label. These chang...

journal_title：Biophysical journal

authors： Miake-Lye RC,Doniach S,Hodgson KO

更新日期：1983-03-01 00:00:00

abstract：:It has been repeatedly noted that the change of conformation of the molecules that serve as the ion-selective channels for sodium and potassium conductance in the nerve membrane will be accompanied by a change in the dipole moment of the molecule. This time-dependent change of dipole moment will produce transient curr...

journal_title：Biophysical journal

authors： Strandberg MW

更新日期：1977-09-01 00:00:00

abstract：:Cells within fibrocartilaginous tissues, including chondrocytes and fibroblasts of the meniscus, ligament, and tendon, regulate cell biosynthesis in response to local mechanical stimuli. The processes by which an applied mechanical load is transferred through the extracellular matrix to the environment of a cell are n...

journal_title：Biophysical journal

authors： Upton ML,Gilchrist CL,Guilak F,Setton LA

更新日期：2008-08-01 00:00:00