Properties of gap junction channels formed by Cx46 alone and in combination with Cx50.

abstract：:Actin is a ubiquitous protein in eukaryotic cells. It plays a major role in cell motility and in the maintenance and control of cell shape. In this article, we intend to address the contribution of actin to the passive mechanical properties of human neutrophils. As a framework for assessing this contribution, the neut...

journal_title：Biophysical journal

authors： Tsai MA,Frank RS,Waugh RE

更新日期：1994-06-01 00:00:00

abstract：:We extend the Saffman theory of membrane hydrodynamics to account for the correlated motion of membrane proteins, along with the effect of protein concentration on that correlation and on the response of the membrane to stresses. Expressions for the coupling diffusion coefficients of protein pairs and their concentrat...

journal_title：Biophysical journal

authors： Oppenheimer N,Diamant H

更新日期：2009-04-22 00:00:00

abstract：:To determine if hydrophobic surfactant proteins affect the stability of pulmonary surfactant monolayers at an air/water interface, the studies reported here compared the kinetics of collapse for the complete set of lipids in calf surfactant with and without the proteins. Monomolecular films spread at the surface of ca...

journal_title：Biophysical journal

authors： Lhert F,Yan W,Biswas SC,Hall SB

更新日期：2007-12-15 00:00:00

abstract：:Molecular motors are thought to generate force and directional motion via nonequilibrium switching between energy surfaces. Because all enzymes can undergo such switching, we hypothesized that the ability to generate rotary motion and torque is not unique to highly adapted biological motor proteins but is instead a co...

journal_title：Biophysical journal

authors： Slochower DR,Gilson MK

更新日期：2018-05-08 00:00:00

abstract：:The presence of two liquid-crystalline phases, alpha and beta, in mixed bilayers of dimyristoylphosphatidylcholine/cholesterol was detected by the changes in the distribution of the fluorescence lifetimes of t-PnA, as analyzed by the Maximum Entropy Method. The formation of the liquid-ordered beta-phase, in the 30-40 ...

journal_title：Biophysical journal

authors： Reyes Mateo C,Ulises Acuña A,Brochon JC

更新日期：1995-03-01 00:00:00

abstract：:We explore the means by which immobilization of a substrate on a surface can increase the rate of a diffusion-controlled enzymatic reaction. A quasichemical approach is developed and compared with Brownian dynamics simulations. We use these methods to show that restricting only the orientation of the enzyme by long-ra...

journal_title：Biophysical journal

authors： Nag A,Dinner AR

更新日期：2006-02-01 00:00:00

abstract：:Cytochrome c3 (cyt c3) can mediate electron transport across phosphatidylcholine (PC)/cardiolipin (CL) and PC/phosphatidylglycerol (PG) membranes. A two-molecule process is involved in the electron transport across PC/CL membranes in the liquid-crystalline state. In contrast, a single-molecule process dominates the el...

journal_title：Biophysical journal

authors： Kim SM,Yamamoto T,Todokoro Y,Takayama Y,Fujiwara T,Park JS,Akutsu H

更新日期：2006-01-15 00:00:00

abstract：:Orientational order parameters of two diphenylhexatriene (DPH)-based fluorescent probes, 2-(3-(diphenylhexatrienyl)propanoyl)-1-hexadecanoyl-sn-glycero-3-p hosphocholine (DPHpPC) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH), in dipalmitoylphosphatidylcholine (DPPC) Langmuir-Blodgett monolayers...

journal_title：Biophysical journal

authors： Zhai X,Kleijn JM

更新日期：1997-06-01 00:00:00

abstract：:The prion protein (PrP), widely recognized to misfold into the causative agent of the transmissible spongiform encephalopathies, has previously been shown to bind to lipid membranes with binding influenced by both membrane composition and pH. Aside from the misfolding events associated with prion pathogenesis, PrP can...

journal_title：Biophysical journal

authors： Le Brun AP,Haigh CL,Drew SC,James M,Boland MP,Collins SJ

更新日期：2014-11-18 00:00:00

abstract：:The efficiency of various patterns of pulsatile stimulation is determined in a model in which a receptor becomes desensitized in the presence of its stimulatory ligand. The effect of stochastic or chaotic changes in the duration and/or interval between successive pulses in a series of square-wave stimuli is investigat...

journal_title：Biophysical journal

authors： Li Y,Goldbeter A

更新日期：1992-01-01 00:00:00

abstract：:The mating pathway in Saccharomyces cerevisiae is one of the best understood signal transduction pathways in eukaryotes. It transmits the mating signal from plasma membrane into the nucleus through the G-protein coupled receptor and the mitogen-activated protein kinase (MAPK) cascade. According to current understandin...

journal_title：Biophysical journal

authors： Shao D,Zheng W,Qiu W,Ouyang Q,Tang C

更新日期：2006-12-01 00:00:00

abstract：:A method for determination of the orientation of adsorbed structure-stable proteins using Total Internal Reflection Fluorescence is outlined. The theory has been elaborated for orientation studies on adsorbed free base cytochrome c, of which the prophyrin can be used as an intrinsic fluorescent label. The ratio of flu...

journal_title：Biophysical journal

authors： Fraaije JG,Kleijn JM,van der Graaf M,Dijt JC

更新日期：1990-05-01 00:00:00

abstract：:Human apolipoprotein E (apoE) is a 299-amino-acid protein with a molecular weight of 34 kDa. The difference between the apoE3 and apoE4 isoforms is a single residue substitution involving a Cys-Arg replacement at residue 112. ApoE4 is positively associated with atherosclerosis and late-onset and sporadic Alzheimer's d...

journal_title：Biophysical journal

authors： Chou CY,Lin YL,Huang YC,Sheu SY,Lin TH,Tsay HJ,Chang GG,Shiao MS

更新日期：2005-01-01 00:00:00

abstract：:Solid-state NMR study shows that the 22-residue K3 peptide (Ser(20)-Lys(41)) from beta(2)-microglobulin (beta(2)m) adopts a beta-strand-loop-beta-strand conformation in its fibril state. Residue Pro(32) has a trans conformation in the fibril state of the peptide, while it adopts a cis conformation in the native state ...

journal_title：Biophysical journal

authors： Liang C,Derreumaux P,Mousseau N,Wei G

更新日期：2008-07-01 00:00:00

abstract：:The dependence of phospholipid vesicle size on lipid composition is investigated by photon correlation spectroscopy. For each lipid composition prolonged ultracentrifugation was used to isolate a nearly uniform population of minimum-sized vesicles. The residual size variations in the samples were sufficient to cause p...

journal_title：Biophysical journal

authors： Goll J,Carlson FD,Barenholz Y,Litman BJ,Thompson TE

更新日期：1982-04-01 00:00:00

abstract：:High-efficiency electrofusion between cells of different sizes was achieved by application of fusing electric pulses to cells in centrifuged pellets. Larger target cells (Chinese hamster ovary or L1210 cells) were stacked among smaller human erythrocytes or erythrocyte ghosts by sequential centrifugation at 700 g to f...

journal_title：Biophysical journal

authors： Li LH,Hensen ML,Zhao YL,Hui SW

更新日期：1996-07-01 00:00:00

abstract：:In total internal reflection fluorescence microscopy (TIRFM), fluorophores near a surface can be excited with evanescent waves, which decay exponentially with distance from the interface. Penetration depths of evanescent waves from 60 nm to 300 nm were generated by varying the angle of incidence of a laser beam. With ...

journal_title：Biophysical journal

authors： Rohrbach A

更新日期：2000-05-01 00:00:00

abstract：:Pure bending of a membrane bilayer is developed including different properties for each membrane half. Both connected and unconnected bilayer surfaces are treated. The bilayer bending resistance is the resultant of parallel surface compression "resistances." The neutral surface is a function of the upper and lower sur...

journal_title：Biophysical journal

authors： Evans EA

更新日期：1974-12-01 00:00:00

abstract：:This study reports the solid-state NMR spectroscopic characterization of the amino-proximate transmembrane domain (TM-A) of a diverged microsomal delta12-desaturase (CREP-1) in a phospholipid bilayer. A series of TM-A peptides were synthesized with 2H-labeled side chains (Ala-53, -56, and -63, Leu-62, Val-50), and the...

journal_title：Biophysical journal

authors： Gibbons WJ Jr,Karp ES,Cellar NA,Minto RE,Lorigan GA

更新日期：2006-02-15 00:00:00

abstract：:The plasma membrane (PM) serves as the functional interface between a cell and its environment, hosting extracellular signal transduction and nutrient transport among a variety of other processes. To support this extensive functionality, PMs are organized into lateral domains, including ordered, lipid-driven assemblie...

journal_title：Biophysical journal

authors： Levental KR,Lorent JH,Lin X,Skinkle AD,Surma MA,Stockenbojer EA,Gorfe AA,Levental I

更新日期：2016-04-26 00:00:00

abstract：:A model for the Na-K exchange pump was applied to data on Na(+)-loaded frog sartorius muscle, and was used to relate the rate of adenosine triphosphate (ATP) hydrolysis to the electrical properties of the cell membrane. Membrane hyperpolarization was considered to arise from an electrical current which was produced by...

journal_title：Biophysical journal

authors： Rapoport SI

更新日期：1971-08-01 00:00:00

abstract：:Widely disparate viruses enter the host cell through an endocytic pathway and travel the cytoplasm inside an endosome. For the viral genetic material to be delivered into the cytoplasm, these viruses have to escape the endosomal compartment, an event triggered by the conformational changes of viral endosomolytic prote...

journal_title：Biophysical journal

authors： Lagache T,Danos O,Holcman D

更新日期：2012-03-07 00:00:00

abstract：:Intercellular channels formed of members of the gene family of connexins (Cxs) vary from being substantially cation selective to being anion selective. We took advantage of the ability of Cx46 to function as an unopposed hemichannel to examine the basis of Cx charge selectivity. Previously we showed Cx46 hemichannels ...

journal_title：Biophysical journal

authors： Trexler EB,Bukauskas FF,Kronengold J,Bargiello TA,Verselis VK

更新日期：2000-12-01 00:00:00

abstract：:The initial output of a time-resolved macromolecular crystallography experiment is a time-dependent series of difference electron density maps that displays the time-dependent changes in underlying structure as a reaction progresses. The goal is to interpret such data in terms of a small number of crystallographically...

journal_title：Biophysical journal

authors： Kostov KS,Moffat K

更新日期：2011-01-19 00:00:00

abstract：:The CLC family of chloride channels and transporters is a functionally diverse group of proteins important in a wide range of physiological processes. ClC-4 and ClC-5 are localized to endosomes and seem to play roles in the acidification of these compartments. These proteins were recently shown to function as Cl(-)/H(...

journal_title：Biophysical journal

authors： Osteen JD,Mindell JA

更新日期：2008-11-15 00:00:00

abstract：:Plant light-harvesting complex II (LHCII) is the key antenna complex for plant photosynthesis. We present coarse-grained molecular dynamics simulations of monomeric and trimeric LHCII in a realistic thylakoid membrane environment based on the Martini force field. The coarse-grained protein model has been optimized wit...

journal_title：Biophysical journal

authors： Thallmair S,Vainikka PA,Marrink SJ

更新日期：2019-04-23 00:00:00

abstract：:A simulation of the function of the human heart and heart muscle has been developed in the form of a digital computer code. For a given set of values for the input variables, realistic values of the cardiac output variables are predicted. A detailed discussion of the simulation and some results obtained from its appli...

journal_title：Biophysical journal

authors： Hanna WT

更新日期：1973-07-01 00:00:00

abstract：:The mechanical properties of cross-linked microtubule bundles were measured from outer pillar cells isolated from the mammalian inner ear. Measurements were made using a three-point bending test and were incorporated into a mathematical model designed to distinguish between the stiffness contributions from microtubule...

journal_title：Biophysical journal

authors： Tolomeo JA,Holley MC

更新日期：1997-10-01 00:00:00

abstract：:The diffusion of Ca as it converges to the external mouth of a Ca channel is examined. Diffusional limitation on Ca ions entering Ca channels during current flow, cause local extracellular Ca depletions. Such extracellular Ca depletions have been reported in cardiac muscle. The cardiac sarcolemma has a large number of...

journal_title：Biophysical journal

authors： Bers DM,Peskoff A

更新日期：1991-03-01 00:00:00

abstract：:We report that long-chain poly-L-glutamine forms cation-selective channels when incorporated into artificial planar lipid bilayer membranes. The channel was permeable to alkali cations and H(+) ions and virtually impermeable to anions; the selectivity sequence based on the single-channel conductance was H(+) > Cs(+) >...

journal_title：Biophysical journal

authors： Monoi H,Futaki S,Kugimiya S,Minakata H,Yoshihara K

更新日期：2000-06-01 00:00:00