Abeta oligomers and fibrillar aggregates induce different apoptotic pathways in LAN5 neuroblastoma cell cultures.

abstract：:Atomic force microscopy (AFM) is nowadays frequently applied to determine interaction forces between biological molecules. Starting with the detection of the first discrete unbinding forces between ligands and receptors by AFM only several years ago, measurements have become more and more quantitative. At the same tim...

journal_title：Biophysical journal

authors： Willemsen OH,Snel MM,Cambi A,Greve J,De Grooth BG,Figdor CG

更新日期：2000-12-01 00:00:00

abstract：:Bathorhodopsins were prepared by partially (10--15%) photoconverting bovine rhodopsin (11-cis chromophore) or isorhodopsin I (9-cis chromophore) at 77 degrees K; care was taken to avoid establishing photostationary states. The absorption spectra calculated for the bathorhodopsins derived from the two parent pigments a...

journal_title：Biophysical journal

authors： Mao B,Ebrey TG,Crouch R

更新日期：1980-02-01 00:00:00

abstract：:Single molecule force spectroscopy methods, such as optical and magnetic tweezers and atomic force microscopy, have opened up the possibility to study biological processes regulated by force, dynamics of structural conformations of proteins and nucleic acids, and load-dependent kinetics of molecular interactions. Amon...

journal_title：Biophysical journal

authors： Capitanio M,Pavone FS

更新日期：2013-09-17 00:00:00

abstract：:Molecular recognition between biotinylated bovine serum albumin and polyclonal, biotin-directed IG antibodies has been measured directly under various buffer conditions using an atomic force microscope (AFM). It was found that even highly structured molecules such as IgG antibodies preserve their specific affinity to ...

journal_title：Biophysical journal

authors： Dammer U,Hegner M,Anselmetti D,Wagner P,Dreier M,Huber W,Güntherodt HJ

更新日期：1996-05-01 00:00:00

abstract：:Structures of the blood clotting enzyme thrombin complexed with hirugen and two active site inhibitors, RWJ-50353 10080(N-methyl-D-phenylalanyl-N-[5-[(aminoiminomethyl)amino]-1- [[(2-benzothiazolyl)carbonyl]butyl]-L-prolinamide trifluoroacetate hydrate) and RWJ-50215 (N-[4-(aminoiminomethyl)amino-1-[2- (thiazol-2-ylca...

journal_title：Biophysical journal

authors： Matthews JH,Krishnan R,Costanzo MJ,Maryanoff BE,Tulinsky A

更新日期：1996-11-01 00:00:00

abstract：:Formation of low-temperature ordered gel phases in several fully hydrated phosphatidylethanolamines (PEs) and phosphatidylcholines (PCs) with saturated chains as well as in dipalmitoylphosphatidylglycerol (DPPG) was observed by synchrotron x-ray diffraction, microcalorimetry, and densitometry. The diffraction patterns...

journal_title：Biophysical journal

authors： Tenchov B,Koynova R,Rapp G

更新日期：2001-04-01 00:00:00

abstract：:The functional diversity of gap junction intercellular channels arising from the large number of connexin isoforms is significantly increased by heterotypic interactions between members of this family. This is particularly evident in the rectifying behavior of Cx26/Cx32 heterotypic channels (. Proc. Natl. Acad. Sci. U...

journal_title：Biophysical journal

authors： Suchyna TM,Nitsche JM,Chilton M,Harris AL,Veenstra RD,Nicholson BJ

更新日期：1999-12-01 00:00:00

abstract：:A Ca(2+) spark arises when a cluster of sarcoplasmic reticulum (SR) channels (ryanodine receptors or RyRs) opens to release calcium in a locally regenerative manner. Normally triggered by Ca(2+) influx across the sarcolemmal or transverse tubule membrane neighboring the cluster, the Ca(2+) spark has been shown to be t...

journal_title：Biophysical journal

authors： Sobie EA,Dilly KW,dos Santos Cruz J,Lederer WJ,Jafri MS

更新日期：2002-07-01 00:00:00

abstract：:A new Fourier series representation of supercoiled DNA is employed in Langevin dynamics simulations to study large-scale configurational motions of intermediate-length chains. The polymer is modeled as an ideal elastic rod subject to long-range van der Waals' interactions. The van der Waals' term prevents the self-con...

journal_title：Biophysical journal

authors： Liu G,Schlick T,Olson AJ,Olson WK

更新日期：1997-10-01 00:00:00

abstract：:Molecular machines fueled by NTP play pivotal roles in a wide range of cellular activities. One common feature among NTP-driven molecular machines is that NTP binding is a major force-generating step among the elementary reaction steps comprising NTP hydrolysis. To understand the mechanism in detail,in this study, we ...

journal_title：Biophysical journal

authors： Arai HC,Yukawa A,Iwatate RJ,Kamiya M,Watanabe R,Urano Y,Noji H

更新日期：2014-07-01 00:00:00

abstract：:Polarized Fourier transform infrared spectroscopy has been used to study the structure of purple membrane from Halobacterium halobium. Membranes were oriented by drying a suspension of membrane fragments onto Irtran-4 slides. Dichroism measurements of the amide I, II and A peaks were used to find the average spatial o...

journal_title：Biophysical journal

authors： Rothschild KJ,Clark NA

更新日期：1979-03-01 00:00:00

abstract：:Electrorotation of fixed red blood cells has been investigated in the frequency range between 16 Hz and 30 MHz. The rotation was studied as a function of electrolyte conductivity and surface charge density. Between 16 Hz and 1 kHz, fixed red blood cells undergo cofield rotation. The maximum of cofield rotation occurs ...

journal_title：Biophysical journal

authors： Georgieva R,Neu B,Shilov VM,Knippel E,Budde A,Latza R,Donath E,Kiesewetter H,Bäumler H

更新日期：1998-04-01 00:00:00

abstract：:We have chemically modified a fraction of the monomers in actin filaments, and then measured the effects on the functional interaction of myosin with unmodified monomers within the same filament. Two modifications were used: (a) covalent attachment of various amounts of myosin subfragment-1 (S1) with the bifunctional ...

journal_title：Biophysical journal

authors： Prochniewicz E,Katayama E,Yanagida T,Thomas DD

更新日期：1993-07-01 00:00:00

abstract：:Polarized photolysis experiments have been performed on the carbon monoxide complex of myoglobin to assess the effects of photoselection on the kinetics of ligand rebinding and to investigate the reorientational dynamics of the heme plane. The results are analyzed in terms of the optical theory developed in the preced...

journal_title：Biophysical journal

authors： Ansari A,Jones CM,Henry ER,Hofrichter J,Eaton WA

更新日期：1993-03-01 00:00:00

abstract：:The pores of ion channel proteins are often modeled as static structures. In this view, selectivity reflects rigidly constrained backbone orientations. Such a picture is at variance with the generalization that biological proteins are flexible, capable of major internal motions on biologically relevant time scales. We...

journal_title：Biophysical journal

authors： Bénitah JP,Ranjan R,Yamagishi T,Janecki M,Tomaselli GF,Marban E

更新日期：1997-08-01 00:00:00

abstract：:Neurons communicate by releasing neurotransmitters that are stored in intracellular vesicular compartments. PC12 cells are frequently used as a model secretory cell line that is described to have two subpools of vesicles: small clear vesicles and dense core vesicles. We measured transmitter molecules released from ves...

journal_title：Biophysical journal

authors： Adams RD,Harkins AB

更新日期：2014-12-16 00:00:00

abstract：:Recent development of titratable coions has paved the way for realizing all-atom molecular dynamics at constant pH. To further improve physical realism, here we describe a technique in which proton titration of the solute is directly coupled to the interconversion between water and hydroxide or hydronium. We test the ...

journal_title：Biophysical journal

authors： Chen W,Wallace JA,Yue Z,Shen JK

更新日期：2013-08-20 00:00:00

abstract：:We measure the organization and substructure of HT29 epithelial cells in a monolayer using angle-resolved low-coherence interferometry. This new technique probes cellular structure by measuring scattered light, as in flow cytometry, but offers an advantage in that the structure can be examined in situ, avoiding the ne...

journal_title：Biophysical journal

authors： Wax A,Yang C,Backman V,Badizadegan K,Boone CW,Dasari RR,Feld MS

更新日期：2002-04-01 00:00:00

abstract：:Fusion is a crucial event in the infection of animal cells by enveloped viruses (e.g., HIV or influenza). Viral fusion is mediated by glycoproteins, spanning the viral envelope, which attach to a membrane surface and induce fusion of the viral envelope to the cellular membrane. Influenza fusion protein (hemagglutinin)...

journal_title：Biophysical journal

authors： Longo ML,Waring AJ,Hammer DA

更新日期：1997-09-01 00:00:00

abstract：:Protein-mediated DNA looping, such as that induced by the lactose repressor (LacI) of Escherichia coli, is a well-known gene regulation mechanism. Although researchers have given considerable attention to DNA looping by LacI, many unanswered questions about this mechanism, including the role of protein flexibility, re...

journal_title：Biophysical journal

authors： Hirsh AD,Lillian TD,Lionberger TA,Perkins NC

更新日期：2011-08-03 00:00:00

abstract：:The outer membrane (OM) of Gram-negative bacteria is composed of lipopolysaccharide (LPS) in the outer leaflet and phospholipids in the inner leaflet. The outer membrane protein H (OprH) of Pseudomonas aeruginosa provides an increased stability to the OMs by directly interacting with LPS. Here we report the influence ...

journal_title：Biophysical journal

authors： Lee J,Patel DS,Kucharska I,Tamm LK,Im W

更新日期：2017-01-24 00:00:00

abstract：:We carried out experiments on single isolated myofibrils in which thin filaments had been functionally removed, leaving the connecting (titin) filaments as the sole agent taking up the length change. With technical advances that gave sub-nanometer detectability we examined the time course of single sarcomere-length ch...

journal_title：Biophysical journal

authors： Blyakhman F,Tourovskaya A,Pollack GH

更新日期：2001-08-01 00:00:00

abstract：:The depth of insertion of an antimicrobial peptide, protegrin-1 (PG-1), in lipid bilayers is investigated using solid-state NMR. Paramagnetic Mn(2+) ions bind to the surface of lipid bilayers and induce distance-dependent dipolar relaxation of nuclear spins. By comparing the signal dephasing of the peptide with that o...

journal_title：Biophysical journal

authors： Buffy JJ,Hong T,Yamaguchi S,Waring AJ,Lehrer RI,Hong M

更新日期：2003-10-01 00:00:00

abstract：:Hypothetical hydroxide and proton migration along the linear water chain in Aquaporin GlpF from Escherichia coli are studied by ab initio Car-Parrinello molecular dynamics simulations. It is found that the protein stabilizes a bipolar single file of water. The single file features a contiguous set of water-water hydro...

journal_title：Biophysical journal

authors： Jensen MØ,Röthlisberger U,Rovira C

更新日期：2005-09-01 00:00:00

abstract：:In the last decades, it has been demonstrated that many animal species orient in the Earth magnetic field. One of the best-studied examples is the use of the geomagnetic field by migratory birds for orientation and navigation. However, the biophysical mechanism underlying animal magnetoreception is still not understoo...

journal_title：Biophysical journal

authors： Solov'yov IA,Schulten K

更新日期：2009-06-17 00:00:00

abstract：:The average sizes of fluid and gel domains in the two-component, two-phase system formed from mixtures of dimyristoyl phosphatidylcholine and distearoyl phosphatidylcholine were determined from an analysis of the electron spin resonance spectral lineshapes of a dimyristoyl phosphatidylcholine-nitroxide spin label as a...

journal_title：Biophysical journal

authors： Sankaram MB,Marsh D,Thompson TE

更新日期：1992-08-01 00:00:00

abstract：:In the plant blue-light sensor phototropin, illumination of the chromophoric LOV domains causes activation of the serine/threonine kinase domain. Flavin mononucleotide (FMN) is a chromophore molecule in the two LOV domains (LOV1 and LOV2), but only LOV2 is responsible for kinase activation. Previous studies reported a...

journal_title：Biophysical journal

authors： Yamamoto A,Iwata T,Sato Y,Matsuoka D,Tokutomi S,Kandori H

更新日期：2009-04-08 00:00:00

abstract：:Fundamental mechanisms governing cell size control and homeostasis are still poorly understood. The relationship between sizes at division and birth in single cells is used as a metric to categorize the basis of size homeostasis. Cells dividing at a fixed size regardless of birth size (sizer) are expected to show a di...

journal_title：Biophysical journal

authors： Facchetti G,Knapp B,Chang F,Howard M

更新日期：2019-11-05 00:00:00

abstract：:Membranes of the lens of the eye of mammals have two particular characteristics, high concentrations of sphingomyelin, and dihydrosphingomyelin and cholesterol. We have studied the miscibility of cholesterol with both egg sphingomyelin and with dihydrosphingomyelin made by hydrogenation of egg sphingomyelin. At a chol...

journal_title：Biophysical journal

authors： Epand RM

更新日期：2003-05-01 00:00:00

abstract：:The primary processes of the photochemical cycle of light-adapted bacteriorhodopsin (BR) were studied by various experimental techniques with a time resolution of 5 x 10(-13) s. The following results were obtained. (a) After optical excitation the first excited singlet state S(1) of bacteriorhodopsin is observed via i...

journal_title：Biophysical journal

authors： Polland HJ,Franz MA,Zinth W,Kaiser W,Kölling E,Oesterhelt D

更新日期：1986-03-01 00:00:00