Abstract:
:Bathorhodopsins were prepared by partially (10--15%) photoconverting bovine rhodopsin (11-cis chromophore) or isorhodopsin I (9-cis chromophore) at 77 degrees K; care was taken to avoid establishing photostationary states. The absorption spectra calculated for the bathorhodopsins derived from the two parent pigments are identical in their lambda max 'S, bandwidths, and extinction coefficients. This result provides further support for the hypothesis that bathorhodopsin is a common intermediate between an 11-cis pigment (rhodopsin) and a 9-cis one (isorhodopsin I) and thus probably has an all-trans chromophore. This in turn is strong evidence for the cis-trans isomerization model of the primary event in vision. The spectrum of the bathoproduct of isorhodopsin II (9,13-dicis chromophore) is different from the other pigments' bathoproducts.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Mao B,Ebrey TG,Crouch Rdoi
10.1016/S0006-3495(80)85129-0subject
Has Abstractpub_date
1980-02-01 00:00:00pages
247-56issue
2eissn
0006-3495issn
1542-0086pii
S0006-3495(80)85129-0journal_volume
29pub_type
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