Single chromatin fiber stretching reveals physically distinct populations of disassembly events.

abstract：:The aggregation of amyloid-β (Aβ) peptides plays a crucial role in the etiology of Alzheimer's disease (AD). Recently, it has been reported that an A2T mutation in Aβ can protect against AD. Interestingly, a nonpolar A2V mutation also has been found to offer protection against AD in the heterozygous state, although it...

journal_title：Biophysical journal

authors： Das P,Murray B,Belfort G

更新日期：2015-02-03 00:00:00

abstract：:Atrial fibrosis has been implicated in the development and maintenance of atrial arrhythmias, and is characterized by expansion of the extracellular matrix and an increased number of fibroblasts (Fbs). Electrotonic coupling between atrial myocytes and Fbs may contribute to the formation of an arrhythmogenic substrate....

journal_title：Biophysical journal

authors： Maleckar MM,Greenstein JL,Giles WR,Trayanova NA

更新日期：2009-10-21 00:00:00

abstract：:Calmodulin (CaM) is a highly flexible calcium-binding protein that mediates signal transduction through an ability to differentially bind to highly variable binding sequences in target proteins. To identify how binding affects CaM motions, and its relationship to conformational entropy and target peptide sequence, we ...

journal_title：Biophysical journal

authors： Smith DM,Straatsma TP,Squier TC

更新日期：2012-10-03 00:00:00

abstract：:We use optical tweezers microrheology and fluorescence microscopy to apply nonlinear microscale strains to entangled and cross-linked actin networks, and measure the resulting stress and actin filament deformations. We couple nonlinear stress response and relaxation to the velocities and displacements of individual fl...

journal_title：Biophysical journal

authors： Gurmessa B,Ricketts S,Robertson-Anderson RM

更新日期：2017-10-03 00:00:00

abstract：:The general theory (Levitt, D. G. 1990. Biophys. J. 59:271-277) is applied to a model channel that resembles the acetylcholine receptor channel (ACH). The model incorporates the known features of the ACH geometry and fixed charge locations. The channel has a wide mouth facing the outer solution, tapering to a narrow r...

journal_title：Biophysical journal

authors： Levitt DG

更新日期：1991-02-01 00:00:00

abstract：:The cardiac muscle protein titin, responsible for developing passive elasticity and extensibility of muscle, possesses about 40 immunoglobulin-like (Ig) domains in its I-band region. Atomic force microscopy (AFM) and steered molecular dynamics (SMD) have been successfully combined to investigate the reversible unfoldi...

journal_title：Biophysical journal

authors： Gao M,Wilmanns M,Schulten K

更新日期：2002-12-01 00:00:00

abstract：:Titin is a very large (>3 MDa) protein found in striated muscle where it is believed to participate in myogenesis and passive tension. A prominent feature in the A-band portion of titin is the presence of an 11-domain super-repeat of immunoglobulin superfamily and fibronectin-type-III-like domains. Seven overlapping c...

journal_title：Biophysical journal

authors： Head JG,Houmeida A,Knight PJ,Clarke AR,Trinick J,Brady RL

更新日期：2001-09-01 00:00:00

abstract：:Antifreeze proteins (AFPs) protect certain organisms from freezing by adhering to ice crystals, thereby preventing their growth. All AFPs depress the nonequilibrium freezing temperature below the melting point; however AFPs from overwintering insects, such as the spruce budworm (sbw) are 10-100 times more effective th...

journal_title：Biophysical journal

authors： Pertaya N,Marshall CB,Celik Y,Davies PL,Braslavsky I

更新日期：2008-07-01 00:00:00

abstract：:New experimental techniques, especially in the context of observing molecular dynamics, reveal the plasma membrane to be heterogeneous and "scale rich," from nanometers to microns and from microseconds to seconds. This is critical information, which shows that scale-dependent transport governs the molecular encounters...

journal_title：Biophysical journal

authors： Lyman E,Hsieh CL,Eggeling C

更新日期：2018-08-21 00:00:00

abstract：:The widely used hydrophobic cannabinoid ligand CP-55,940 partitions with high efficiency into biomembranes. We studied the location, orientation, and dynamics of CP-55,940 in POPC bilayers by solid-state NMR. Chemical-shift perturbation of POPC protons from the aromatic ring-current effect, as well as 1H NMR cross-rel...

journal_title：Biophysical journal

authors： Kimura T,Cheng K,Rice KC,Gawrisch K

更新日期：2009-06-17 00:00:00

abstract：:We report here a multiprotein blind test of a computer method to predict native protein structures based solely on an all-atom physics-based force field. We use the AMBER 96 potential function with an implicit (GB/SA) model of solvation, combined with replica-exchange molecular-dynamics simulations. Coarse conformatio...

journal_title：Biophysical journal

authors： Shell MS,Ozkan SB,Voelz V,Wu GA,Dill KA

更新日期：2009-02-01 00:00:00

abstract：:An ab initio model for gene prediction in prokaryotic genomes is proposed based on physicochemical characteristics of codons calculated from molecular dynamics (MD) simulations. The model requires a specification of three calculated quantities for each codon: the double-helical trinucleotide base pairing energy, the b...

journal_title：Biophysical journal

authors： Singhal P,Jayaram B,Dixit SB,Beveridge DL

更新日期：2008-06-01 00:00:00

abstract：:Glutamic acid [(L-glu)n] + dihydrogen phosphate systems are studied by infrared (IR) spectroscopy dried and hydrated at 75% relative humidity, as a function of both the phosphate-glutamic acid residue (Pi/glu) ratio and the type of cations present. It is shown that the glutamic acid groups form hydrogen-bonded chains ...

journal_title：Biophysical journal

authors： Burget U,Zundel G

更新日期：1987-12-01 00:00:00

abstract：:In cardiac tissue, during partial blockade of the membrane sodium channels, or at high frequencies of excitation, inexcitable obstacles with sharp edges may destabilize the propagation of electrical excitation waves, causing the formation of self-sustained vortices and turbulent cardiac electrical activity. The format...

journal_title：Biophysical journal

authors： Cabo C,Pertsov AM,Davidenko JM,Baxter WT,Gray RA,Jalife J

更新日期：1996-03-01 00:00:00

abstract：:The early events in halorhodopsin after light excitation are studied with picosecond time resolution. Absorption and fluorescence measurements show that the electronically excited state of the incorporated retinal has a lifetime of 5 ps. Within that time a red-shifted photoproduct is formed that remains stable for at ...

journal_title：Biophysical journal

authors： Polland HJ,Franz MA,Zinth W,Kaiser W,Hegemann P,Oesterhelt D

更新日期：1985-01-01 00:00:00

abstract：:Far infrared (FIR) spectral measurements of wild-type (WT) and D96N mutant bacteriorhodopsin thin films have been carried out using terahertz time domain spectroscopy as a function of hydration, temperature, and conformational state. The results are compared to calculated spectra generated via normal mode analyses usi...

journal_title：Biophysical journal

authors： Whitmire SE,Wolpert D,Markelz AG,Hillebrecht JR,Galan J,Birge RR

更新日期：2003-08-01 00:00:00

abstract：:Actin, a highly conserved cytoskeletal protein found in all eukaryotic cells, facilitates cell motility and membrane remodeling via a directional polymerization cycle referred to as treadmilling. The nucleotide bound at the core of each actin subunit regulates this process. Although the biochemical kinetics of treadmi...

journal_title：Biophysical journal

authors： Saunders MG,Tempkin J,Weare J,Dinner AR,Roux B,Voth GA

更新日期：2014-04-15 00:00:00

abstract：:Heterogeneity of ADP diffusion and regulation of respiration were studied in permeabilized cardiomyocytes and cardiac fibers in situ and in silico. Regular arrangement of mitochondria in cells was altered by short-time treatment with trypsin and visualized by confocal microscopy. Manipulation of matrix volumes by chan...

journal_title：Biophysical journal

authors： Saks V,Kuznetsov A,Andrienko T,Usson Y,Appaix F,Guerrero K,Kaambre T,Sikk P,Lemba M,Vendelin M

更新日期：2003-05-01 00:00:00

abstract：:The goal of this paper is to examine the origins and relative importance of primary and secondary sources of electric and magnetic fields for excitable tissue. It is shown that for axonal and cardiac tissue a comparison of the relative field strength from both primary and secondary sources shows only the latter to be ...

journal_title：Biophysical journal

authors： Plonsey R

更新日期：1982-09-01 00:00:00

abstract：:Dynamic properties of class A beta-lactamase TEM-1 are investigated from molecular dynamics (MD) simulations. Comparison of MD-derived order parameters with those obtained from model-free analysis of nuclear magnetic resonance (NMR) relaxation data shows high agreement for N-H moieties within alpha- and beta-secondary...

journal_title：Biophysical journal

authors： Fisette O,Morin S,Savard PY,Lagüe P,Gagné SM

更新日期：2010-02-17 00:00:00

abstract：:Glucagon solutions at pH 2.0 were subjected to mechanical agitation at 37 degrees C in the presence of a hydrophobic surface to explore the details of aggregation and fiber formation. High-resolution intermittent-contact atomic force microscopy performed in solution revealed the presence of aggregates after 0.5 h; how...

journal_title：Biophysical journal

authors： De Jong KL,Incledon B,Yip CM,DeFelippis MR

更新日期：2006-09-01 00:00:00

abstract：:Various differential polarization images or Mueller images of model objects are generated using the equations derived in the previous paper (paper I of this series). These calculated images include models of the higher-order organization of metaphase chromosomes, and show the applicability of the differential polariza...

journal_title：Biophysical journal

authors： Kim M,Ulibarri L,Bustamante C

更新日期：1987-12-01 00:00:00

abstract：:There is a steadily growing body of experimental data describing the diffusion of acetylcholine in the neuromuscular junction and the subsequent miniature endplate currents produced at the postsynaptic membrane. To gain further insights into the structural features governing synaptic transmission, we have performed ca...

journal_title：Biophysical journal

authors： Smart JL,McCammon JA

更新日期：1998-10-01 00:00:00

abstract：:We report the deposition of lipid monolayers and bilayers on polyacrylamide films deposited by radical chain reaction onto solid substrates in aqueous solutions. Polymer films of various degrees of monomer density and cross-linking are prepared. Lateral diffusion and fluorescent probe permeation measurements yield ins...

journal_title：Biophysical journal

authors： Kühner M,Tampé R,Sackmann E

更新日期：1994-07-01 00:00:00

abstract：:Solid-state NMR study shows that the 22-residue K3 peptide (Ser(20)-Lys(41)) from beta(2)-microglobulin (beta(2)m) adopts a beta-strand-loop-beta-strand conformation in its fibril state. Residue Pro(32) has a trans conformation in the fibril state of the peptide, while it adopts a cis conformation in the native state ...

journal_title：Biophysical journal

authors： Liang C,Derreumaux P,Mousseau N,Wei G

更新日期：2008-07-01 00:00:00

abstract：:In this article we present a study of a new class of surfactants denoted as PEG1500-12-acyloxystearates, which have potential use as pharmaceutical solubilizers. These amphiphilic molecules present interesting properties with regard to cell damage effects. PEG1500-12-acyloxystearates with C(14) to C(16) acyloxy chains...

journal_title：Biophysical journal

authors： Vaccaro M,von Corswant C,Söderman O

更新日期：2007-12-15 00:00:00

abstract：:The effects of the Ca(2+)-induced Ca2+ release blocker procaine on individual sarcoplasmic reticulum Ca2+ release channels have been examined in planar lipid bilayers. Procaine did not reduce the single channel conductance nor appreciably shorten the mean open times of the channel; rather, it increased the longest clo...

journal_title：Biophysical journal

authors： Zahradníková A,Palade P

更新日期：1993-04-01 00:00:00

abstract：:Tissues and cells sustain recurring mechanical loads that span a wide range of loading amplitudes and timescales as a consequence of exposure to blood flow, muscle activity, and external impact. Both tissues and cells derive their mechanical strength from fibrous protein scaffolds, which typically have a complex hiera...

journal_title：Biophysical journal

authors： Kurniawan NA,Vos BE,Biebricher A,Wuite GJ,Peterman EJ,Koenderink GH

更新日期：2016-09-06 00:00:00

abstract：:Structural rearrangements of the myosin upper-50 kD subdomain are thought to play a key role in coordinating actin binding with nucleotide hydrolysis during the myosin ATPase cycle. Such rearrangements could open and close the active site in opposition to the actin-binding cleft, helping explain the opposing affinitie...

journal_title：Biophysical journal

authors： Robertson CI,Gaffney DP 2nd,Chrin LR,Berger CL

更新日期：2005-09-01 00:00:00

abstract：:We report molecular dynamics simulations in the explicit membrane environment of a small membrane-embedded protein, sarcolipin, which regulates the sarcoplasmic reticulum Ca-ATPase activity in both cardiac and skeletal muscle. In its monomeric form, we found that sarcolipin adopts a helical conformation, with a comput...

journal_title：Biophysical journal

authors： Shi L,Cembran A,Gao J,Veglia G

更新日期：2009-05-06 00:00:00