Differential polarization imaging. II. Symmetry properties and calculations.

abstract：:The effects of Hofmeister anions on the perpendicular and lateral equation-of-state (EOS) of the dipalmitoylphosphatidylcholine lamellar phase discussed in the companion article are here examined using appropriate free energy models for the intra- and interbilayer interactions. Minimizing the free energy with respect ...

journal_title：Biophysical journal

authors： Leontidis E,Aroti A,Belloni L,Dubois M,Zemb T

更新日期：2007-09-01 00:00:00

abstract：:Exceptions to the Meyer-Overton rule are commonly cited as evidence against indirect, membrane-mediated mechanisms of general anesthesia. However, another interpretation is possible within the context of an indirect mechanism in which solubilization of an anesthetic in the membrane causes a redistribution of lateral p...

journal_title：Biophysical journal

authors： Cantor RS

更新日期：2001-05-01 00:00:00

abstract：:The yolk granule is the most abundant membrane-bound organelle present in sea urchin eggs and embryos. The major protein component of this organelle, toposome, accounts for approximately 50% of the total yolk protein and has been shown to be localized to the embryonic cell surface. Extensive characterization in severa...

journal_title：Biophysical journal

authors： Hayley M,Emberley J,Davis PJ,Morrow MR,Robinson JJ

更新日期：2006-12-15 00:00:00

abstract：:Single molecule force spectroscopy methods, such as optical and magnetic tweezers and atomic force microscopy, have opened up the possibility to study biological processes regulated by force, dynamics of structural conformations of proteins and nucleic acids, and load-dependent kinetics of molecular interactions. Amon...

journal_title：Biophysical journal

authors： Capitanio M,Pavone FS

更新日期：2013-09-17 00:00:00

abstract：:We present a model of cell motility generated by actomyosin contraction of the cell cortex. We identify, analytically, dynamical instabilities of the cortex and show that they yield steady-state cortical flows, which, in turn, can induce cell migration in three-dimensional environments. This mechanism relies on the re...

journal_title：Biophysical journal

authors： Hawkins RJ,Poincloux R,Bénichou O,Piel M,Chavrier P,Voituriez R

更新日期：2011-09-07 00:00:00

abstract：:High speed cinemicrographs were made of sea urchin sperm at temperatures varying from 22 to 6 degrees C. Apparatus, combining a television camera and a video digitizer, was constructed to scan individual flagellar images and to digitize the flagellar waveforms. With appropriate smoothing and averaging procedures, the ...

journal_title：Biophysical journal

authors： Rikmenspoel R,Isles CA

更新日期：1985-03-01 00:00:00

abstract：:We have developed a saturation transfer EPR (ST-EPR) method to measure selectively the rotational dynamics of those lipids that are motionally restricted by integral membrane proteins and have applied this methodology to measure lipid-protein interactions in native sarcoplasmic reticulum (SR) membranes. This analysis ...

journal_title：Biophysical journal

authors： Squier TC,Thomas DD

更新日期：1989-10-01 00:00:00

abstract：:Intracellular cargo transport frequently involves multiple motor types, either having opposite directionality or having the same directionality but different speeds. Although significant progress has been made in characterizing kinesin motors at the single-molecule level, predicting their ensemble behavior is challeng...

journal_title：Biophysical journal

authors： Arpağ G,Shastry S,Hancock WO,Tüzel E

更新日期：2014-10-21 00:00:00

abstract：:The spatiotemporal organization and dynamics of the plasma membrane and its constituents are central to cellular function. Fluorescence-based single-particle tracking has emerged as a powerful approach for studying the single molecule behavior of plasma-membrane-associated events because of its excellent background su...

journal_title：Biophysical journal

authors： de Wit G,Albrecht D,Ewers H,Kukura P

更新日期：2018-06-19 00:00:00

abstract：:The extracellular matrix contains components with remarkable mechanical properties, including fibronectin (Fn) fibers with extensibilities of >700% strain. We utilized what we consider a novel technique to quantify the extent of molecular unfolding that contributes to Fn fiber extension, and we compared this behavior ...

journal_title：Biophysical journal

authors： Bradshaw MJ,Smith ML

更新日期：2011-10-05 00:00:00

abstract：:Ca(2+) ion effect on protoplasmic streaming in an internodal cell of Nitella has been investigated for various temperatures. We have found that the protoplasmic streaming at low temperature is remarkably affected by the Ca(2+) ions in the internodal cell but larger concentrations of the Ca(2+) ions are needed to suppr...

journal_title：Biophysical journal

authors： Takamatsu A,Aoki T,Tsuchiya Y

更新日期：1993-01-01 00:00:00

abstract：:Actin labeled at Gln-41 with dansyl ethylenediamine (DED) via transglutaminase reaction was used for monitoring the interaction of myosin subfragment 1 (S1) with the His-40-Gly-42 site in the 38-52 loop on F-actin. Proteolytic digestions of F-actin with subtilisin and trypsin, and acto-S1 ATPase measurements on heat-t...

journal_title：Biophysical journal

authors： Kim E,Miller CJ,Motoki M,Seguro K,Muhlrad A,Reisler E

更新日期：1996-03-01 00:00:00

abstract：:Previously, we discussed the calculation of the dipole moments of small proteins using the three-dimensional protein data-base. Our results demonstrate that the calculated dipole moments are in acceptable agreement with measured values. We, however, noted the difficulty of the calculation with larger proteins, in part...

journal_title：Biophysical journal

authors： Takashima S

更新日期：1993-05-01 00:00:00

abstract：:Concentrated solutions of bovine beta-lactoglobulin were studied using osmotic stress and rheological techniques. At pH 6.0 and 8.0, the osmotic pressure was largely independent of NaCl concentration and could be described by a hard sphere equation of state. At pH 5.1, close to the isoelectric point, the osmotic press...

journal_title：Biophysical journal

authors： Parker R,Noel TR,Brownsey GJ,Laos K,Ring SG

更新日期：2005-08-01 00:00:00

abstract：:In the outer hair cell (OHC), the extracisternal space (ECiS) is a conduit and reservoir of the molecular and ionic substrates of the lateral wall, including those necessary for electromotility. To determine the mechanisms through which molecules are transported in the ECiS of the OHC, we selectively imaged the time-d...

journal_title：Biophysical journal

authors： Gliko O,Saggau P,Brownell WE

更新日期：2009-08-19 00:00:00

abstract：:A kinetic model of the effect of agonist and anesthetics on ligand-gated ion channels, developed in earlier work, is further refined and used to predict traces observed in fast-perfusion electrophysiological studies on recombinant GABAA receptors under a wide range of agonist and/or anesthetic concentrations. The mode...

journal_title：Biophysical journal

authors： Lee DK,Albershardt DJ,Cantor RS

更新日期：2015-03-10 00:00:00

abstract：:The 77-K resonance Raman vibrational spectrum of intact goldfish rod photoreceptors containing 3,4-dehydro (A2) retinal is dominated by scattering from the 9-cis component of the steady state at all excitation wavelengths. Intact goldfish photoreceptors were regenerated with an A1-retinal chromophore to determine whet...

journal_title：Biophysical journal

authors： Barry B,Mathies RA,Pardoen JA,Lugtenburg J

更新日期：1987-10-01 00:00:00

abstract：:The fusion peptides of HIV and influenza virus are crucial for viral entry into a host cell. We report the membrane-perturbing and structural properties of fusion peptides from the HA fusion protein of influenza virus and the gp41 fusion protein of HIV. Our goals were to determine: 1), how fusion peptides alter struct...

journal_title：Biophysical journal

authors： Haque ME,Koppaka V,Axelsen PH,Lentz BR

更新日期：2005-11-01 00:00:00

abstract：:The TRPV1 cation nonselective ion channel plays an essential role in thermosensation and perception of other noxious stimuli. TRPV1 can be activated by low extracellular pH, high temperature, or naturally occurring pungent molecules such as allicin, capsaicin, or resiniferatoxin. Its noxious thermal sensitivity makes ...

journal_title：Biophysical journal

authors： Ladrón-de-Guevara E,Dominguez L,Rangel-Yescas GE,Fernández-Velasco DA,Torres-Larios A,Rosenbaum T,Islas LD

更新日期：2020-02-25 00:00:00

abstract：:The mathematics of the binomial model for quantal neurotransmitter release is considered in general terms, to explore what information might be extractable from statistical aspects of data. For an array of N statistically independent release sites, each with a release probability p, the compound binomial always pertai...

journal_title：Biophysical journal

authors： Quastel DM

更新日期：1997-02-01 00:00:00

abstract：:A kinetic model is proposed to reproduce the time courses of the concentration change in paused leader RNA, terminated leader RNA, and readthrough RNA in the single-round transcription experiments on trp leader region of Escherichia coli and its mutants, L132, L75, and L75L135 (Winkler, M. E., and C. Yanofsky, 1981, B...

journal_title：Biophysical journal

authors： Suzuki H,Kunisawa T,Otsuka J

更新日期：1987-03-01 00:00:00

abstract：:In the last decades, it has been demonstrated that many animal species orient in the Earth magnetic field. One of the best-studied examples is the use of the geomagnetic field by migratory birds for orientation and navigation. However, the biophysical mechanism underlying animal magnetoreception is still not understoo...

journal_title：Biophysical journal

authors： Solov'yov IA,Schulten K

更新日期：2009-06-17 00:00:00

abstract：:The heterologous expression and purification of membrane proteins represent major limitations for their functional and structural analysis. Here we describe a new method of incorporation of transmembrane proteins in planar lipid bilayer starting from 1 pmol of solubilized proteins. The principle relies on the direct i...

journal_title：Biophysical journal

authors： Milhiet PE,Gubellini F,Berquand A,Dosset P,Rigaud JL,Le Grimellec C,Lévy D

更新日期：2006-11-01 00:00:00

abstract：:We have used confocal microscopy to examine the [Ca2+]i increase in the albino eggs of the frog Xenopus laevis after fertilization. Eggs were placed in agar wells with their animal poles downward so that fertilization occurred preferentially in the equatorial plane, and confocal microscopy was used to provide a two-di...

journal_title：Biophysical journal

authors： Fontanilla RA,Nuccitelli R

更新日期：1998-10-01 00:00:00

abstract：:DNA represents a promising therapeutic and prophylactic macromolecule in treating genetic diseases, infectious diseases and cancers. The therapeutic potential of DNA is directly related to how DNA transports within the targeted tissue. In this study, fluorescence photobleaching recovery was used to examine the diffusi...

journal_title：Biophysical journal

authors： Shen H,Hu Y,Saltzman WM

更新日期：2006-07-15 00:00:00

abstract：:Oriented solid-state NMR in combination with multiple-residue-specific (15)N labeling and extensive numerical spectral analysis is proposed to determine helix conformations of large membrane proteins in native membranes. The method is demonstrated on uniaxially oriented samples of (15)N-methionine, -valine, and -glyci...

journal_title：Biophysical journal

authors： Vosegaard T,Kamihira-Ishijima M,Watts A,Nielsen NC

更新日期：2008-01-01 00:00:00

abstract：:Variation in the amplitude of miniature postsynaptic currents (mPSCs) generated by individual quanta of neurotransmitter is a major contributor to the variance of evoked synaptic responses. Here we explore the possible origins of this variability by developing a mathematical description of mPSC generation and consider...

journal_title：Biophysical journal

authors： Uteshev VV,Pennefather PS

更新日期：1996-09-01 00:00:00

abstract：:The gelation of sickle hemoglobin includes the formation of spherulitic arrays of polymers, known as polymer domains, which are an intrinsic result of the polymer formation mechanism. We have observed the diffusion of monomers into domains as they form, which substantially increases the total concentration of hemoglob...

journal_title：Biophysical journal

authors： Cho MR,Ferrone FA

更新日期：1990-10-01 00:00:00

abstract：:The giant protein titin is responsible for the elasticity of nonactivated muscle sarcomeres. Titin-based passive stiffness in myocardium is modulated by titin-isoform switching and protein-kinase (PK)A- or PKG-dependent titin phosphorylation. Additional modulatory effects on titin stiffness may arise from disulfide bo...

journal_title：Biophysical journal

authors： Grützner A,Garcia-Manyes S,Kötter S,Badilla CL,Fernandez JM,Linke WA

更新日期：2009-08-05 00:00:00

abstract：:The role of distance-dependent anisotropic reactivity and molecular geometry in the vicinity of localized reaction centers in influencing the rate of bimolecular diffusion-controlled reactions is analyzed in detail, both analytically and numerically. The effect of local molecular shape is considered within the model o...

journal_title：Biophysical journal

authors： Shushin AI,Barzykin AV

更新日期：2001-12-01 00:00:00