Abstract:
:The gelation of sickle hemoglobin includes the formation of spherulitic arrays of polymers, known as polymer domains, which are an intrinsic result of the polymer formation mechanism. We have observed the diffusion of monomers into domains as they form, which substantially increases the total concentration of hemoglobin within the domain. The maximum total concentration attained is comparable with the pellet concentration of 0.5-0.55 g/cm3 obtained in sedimentation experiments. The half time for this process is approximately 50 s for domains of 25 microns radius, and is approximately independent of temperature. The shape of the diffusion progress curves as well as the deduced diffusion constants, and their weak temperature dependence are consistent with a simple model of hemoglobin monomer diffusion into the domain.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Cho MR,Ferrone FAdoi
10.1016/S0006-3495(90)82449-8subject
Has Abstractpub_date
1990-10-01 00:00:00pages
1067-73issue
4eissn
0006-3495issn
1542-0086pii
S0006-3495(90)82449-8journal_volume
58pub_type
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