MreB-Dependent Organization of the E. coli Cytoplasmic Membrane Controls Membrane Protein Diffusion.

abstract：:We have measured the rates of insertion into, desorption from, and spontaneous interlayer translocation (flip-flop) in liquid-disordered and liquid-ordered phase lipid bilayer membranes, of the fluorescent phospholipid derivative NBD-dimyristoylphosphatidyl ethanolamine. This study made use of a recently described met...

journal_title：Biophysical journal

authors： Abreu MS,Moreno MJ,Vaz WL

更新日期：2004-07-01 00:00:00

abstract：:Two isoforms of the sarcoplasmic reticulum Ca2+ release channel (ryanodine receptor or RYR) are expressed together in the skeletal muscles of most vertebrates. We have studied physiological properties of the two isoforms (alpha and beta) by comparing SR preparations from specialized fish muscles that express the alpha...

journal_title：Biophysical journal

authors： O'Brien J,Valdivia HH,Block BA

更新日期：1995-02-01 00:00:00

abstract：:We investigate the permeability of lipid membranes for fluorescence dyes and ions. We find that permeability reaches a maximum close to the chain melting transition of the membranes. Close to transitions, fluctuations in area and compressibility are high, leading to an increased likelihood of spontaneous lipid pore fo...

journal_title：Biophysical journal

authors： Blicher A,Wodzinska K,Fidorra M,Winterhalter M,Heimburg T

更新日期：2009-06-03 00:00:00

abstract：:Actin is one of the main components of the architecture of cells. Actin filaments form different polymer networks with versatile mechanical properties that depend on their spatial organization and the presence of cross-linkers. Here, we investigate the mechanical properties of actin bundles in the absence of cross-lin...

journal_title：Biophysical journal

authors： Rückerl F,Lenz M,Betz T,Manzi J,Martiel JL,Safouane M,Paterski-Boujemaa R,Blanchoin L,Sykes C

更新日期：2017-09-05 00:00:00

abstract：:The analysis of peptide and protein partitioning in lipid membranes is of high relevance for the understanding of biomembrane function. We used statistical thermodynamics analysis to demonstrate the effect of peptide mixing behavior on heat capacity profiles of lipid membranes with the aim to predict peptide aggregati...

journal_title：Biophysical journal

authors： Ivanova VP,Makarov IM,Schäffer TE,Heimburg T

更新日期：2003-04-01 00:00:00

abstract：:The Jun a 3 protein from mountain cedar (Juniperus ashei) pollen, a member of group 5 of the family of plant pathogenesis-related proteins (PR-proteins), reacts with serum IgE from patients with cedar hypersensitivity. We used the crystal structures of two other proteins of this group, thaumatin and an antifungal prot...

journal_title：Biophysical journal

authors： Soman KV,Midoro-Horiuti T,Ferreon JC,Goldblum RM,Brooks EG,Kurosky A,Braun W,Schein CH

更新日期：2000-09-01 00:00:00

abstract：:Evidence from electron microscopy indicates that the separation between adjacent membranes of the central nervous system (CNS) is less than 500 A and perhaps as small as 100-250 A. The rapid K(+) efflux associated with the neural action potential may therefore be sufficient to affect the local extracellular potassium ...

journal_title：Biophysical journal

authors： Lebovitz RM

更新日期：1970-05-01 00:00:00

abstract：:Molecular dynamics simulations of wild-type bacteriorhodopsin (bR) and of its D85N, D85T, D212N, and Y57F mutants have been carried out to investigate possible differences in the photoproducts of these proteins. For each mutant, a series of 50 molecular dynamics simulations of the photoisomerization and subsequent rel...

journal_title：Biophysical journal

authors： Humphrey W,Bamberg E,Schulten K

更新日期：1997-03-01 00:00:00

abstract：:The regulated ability of integrin alphaIIbbeta3 to bind fibrinogen plays a crucial role in platelet aggregation and hemostasis. We have developed a model system based on laser tweezers, enabling us to measure specific rupture forces needed to separate single receptor-ligand complexes. First of all, we performed a thor...

journal_title：Biophysical journal

authors： Litvinov RI,Bennett JS,Weisel JW,Shuman H

更新日期：2005-10-01 00:00:00

abstract：:The pores of ion channel proteins are often modeled as static structures. In this view, selectivity reflects rigidly constrained backbone orientations. Such a picture is at variance with the generalization that biological proteins are flexible, capable of major internal motions on biologically relevant time scales. We...

journal_title：Biophysical journal

authors： Bénitah JP,Ranjan R,Yamagishi T,Janecki M,Tomaselli GF,Marban E

更新日期：1997-08-01 00:00:00

abstract：:The supertwisted, double-stranded, replicative intermediate of øX174 DNA (RFI) has been used to determine whether one of the two strands of the double helix is uniquely sensitive to induction of single-strand breaks by ionizing radiation. This could result from a particularly sensitive base sequence or a transfer of e...

journal_title：Biophysical journal

authors： Achey PM,Courtney DG

更新日期：1973-01-01 00:00:00

abstract：:In rat mesenteric arteries, smooth muscle cells exhibit intercellular calcium waves in response to local phenylephrine stimulation. These waves have a velocity of approximately 20 cells/s and a range of approximately 80 cells. We analyze these waves in a theoretical model of a population of coupled smooth muscle cells...

journal_title：Biophysical journal

authors： Koenigsberger M,Seppey D,Bény JL,Meister JJ

更新日期：2010-07-21 00:00:00

abstract：:A major component of green tea extracts, catechin (-)-Epigallocatechin gallate (EGCg), has been reported to be biologically active and interacting with membranes. A recent study reported drastic effects of EGCg on giant unilamellar vesicles (GUVs). In particular, EGCg above 30 microM caused GUVs to burst. Here we inve...

journal_title：Biophysical journal

authors： Sun Y,Hung WC,Chen FY,Lee CC,Huang HW

更新日期：2009-02-01 00:00:00

abstract：:It has been accepted that the force produced by a skeletal muscle myofibril depends on its cross-sectional area but not on the number of active sarcomeres because they are arranged in series. However, a previous study performed by our group showed that blocking actomyosin interactions within an activated myofibril and...

journal_title：Biophysical journal

authors： Mendoza AC,Rassier DE

更新日期：2020-04-21 00:00:00

abstract：:The microsecond folding and unfolding kinetics of ovine prion proteins (ovPrP) were measured under various solution conditions. A fragment comprising residues 94-233 of the full-length ovPrP was studied for four variants with differing susceptibilities to classical scrapie in sheep. The observed biexponential unfoldin...

journal_title：Biophysical journal

authors： Chen KC,Xu M,Wedemeyer WJ,Roder H

更新日期：2011-09-07 00:00:00

abstract：:Voltage-dependent G-protein inhibition of N-type calcium channels reduces presynaptic calcium entry, sharply attenuating neurotransmitter release. Studies in neurons demonstrate that G-proteins have multiple modulatory effects on N-type channels. The observed changes may reflect genuine complexity in G-protein action ...

journal_title：Biophysical journal

authors： Patil PG,de Leon M,Reed RR,Dubel S,Snutch TP,Yue DT

更新日期：1996-11-01 00:00:00

abstract：:Diffusion of the chloride salts of Li, Na, K, Rb, and Cs from water into 1-butanol and from 1-butanol into water was examined at temperatures from 13-40 degrees C. Distribution coefficients, interfacial transfer coefficients, Arrhenius activation energy, free energy of activation, enthalpy, and entropy of activation w...

journal_title：Biophysical journal

authors： Ting HP,Bertrand GL,Sears DF

更新日期：1966-11-01 00:00:00

abstract：:The biophysical characterization of nonfunctional protein aggregates at physiologically relevant temperatures is much needed to gain deeper insights into the kinetic and thermodynamic relationships between protein folding and misfolding. Dynamic and static laser light scattering have been employed for the detection an...

journal_title：Biophysical journal

authors： Chow C,Kurt N,Murphy RM,Cavagnero S

更新日期：2006-01-01 00:00:00

abstract：:The antagonizing effect of high pressure against anesthesia is well known. With purified firefly luciferase, however,. Biophys. J. 60:1309-1314) reported that high pressure did not affect the initial flash intensity. Firefly luciferase emits a burst of light when the substrates luciferin and ATP are added in the prese...

journal_title：Biophysical journal

authors： Ueda I,Minami H,Matsuki H,Inoue T

更新日期：1999-01-01 00:00:00

abstract：:The polyunsaturated fatty acid docosahexaenoic acid (DHA) makes up approximately 50% of the lipid chains in the retinal rod outer segment disk membranes and a large fraction of the lipid chains in the membranes of neuronal tissues. There is an extensive literature concerned with the dietary requirements for essential ...

journal_title：Biophysical journal

authors： Everts S,Davis JH

更新日期：2000-08-01 00:00:00

abstract：:Object contrast is one of the most important parameters of macromolecular imaging. Low-voltage transmission electron microscopy has shown an increased atom contrast for carbon materials, indicating that amplitude contrast contributions increase at a higher rate than phase contrast and inelastic scattering. Here, we st...

journal_title：Biophysical journal

authors： Majorovits E,Angert I,Kaiser U,Schröder RR

更新日期：2016-02-23 00:00:00

abstract：:Using a method they developed, Stamatoff and Krimm (1976) have phased swelling data from nerve myelin. Although most phases agree with those I determined previously, there are a few differences. In this letter the two different phasings, theirs and my own, are used to compute the corresponding electron-density profile...

journal_title：Biophysical journal

authors： Blaurock AE

更新日期：1979-04-01 00:00:00

abstract：:A partially depolarized squid axon membrane is assumed to have a quasi-steady state negative resistance, the membrane potential is clamped at one point, and a distribution of potential along the axon is obtained from the cable equation. Nominal experimental values of -2 ohm cm(2) for the membrane and 6 ohm cm(2) for t...

journal_title：Biophysical journal

authors： COLE KS

更新日期：1961-05-01 00:00:00

abstract：:Dynamic properties of class A beta-lactamase TEM-1 are investigated from molecular dynamics (MD) simulations. Comparison of MD-derived order parameters with those obtained from model-free analysis of nuclear magnetic resonance (NMR) relaxation data shows high agreement for N-H moieties within alpha- and beta-secondary...

journal_title：Biophysical journal

authors： Fisette O,Morin S,Savard PY,Lagüe P,Gagné SM

更新日期：2010-02-17 00:00:00

abstract：:Small-angle neutron and x-ray scattering experiments have been performed on type 2 tissular transglutaminase to characterize the conformational changes that bring about Ca(2+) activation and guanosine triphosphate (GTP) inhibition. The native and a proteolyzed form of the enzyme, in the presence and in the absence of ...

journal_title：Biophysical journal

authors： Mariani P,Carsughi F,Spinozzi F,Romanzetti S,Meier G,Casadio R,Bergamini CM

更新日期：2000-06-01 00:00:00

abstract：:Adaptation of the attractant response in Escherichia coli is attributable to the methylation of its transmembrane chemotactic receptors by the methyltransferase CheR. This protein contains two binding domains, one for the sites of methylation themselves and the other for a flexible tether at the C terminus of the rece...

journal_title：Biophysical journal

authors： Levin MD,Shimizu TS,Bray D

更新日期：2002-04-01 00:00:00

abstract：:P188, a triblock copolymer of the form poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) helps seal electroporated cell membranes, arresting the leakage of intracellular materials from the damaged cells. To explore the nature of the interaction between P188 and cell membranes, we have constructed a model...

journal_title：Biophysical journal

authors： Maskarinec SA,Hannig J,Lee RC,Lee KY

更新日期：2002-03-01 00:00:00

abstract：:A master equation theory is formulated to describe the dependence of the fluorescence yield (phi) in photosynthetic systems on the number of photons (Y) absorbed per photosynthetic unit (or domain). This theory is applied to the calculation of the dependence of the fluorescence yield on Y in (a) fluorescence induction...

journal_title：Biophysical journal

authors： Paillotin G,Geacintov NE,Breton J

更新日期：1983-10-01 00:00:00

abstract：:Fluorescence recovery after photobleaching (FRAP) has been used previously to investigate the kinetics of binding to biological surfaces. The present study adapts and further develops this technique for the quantification of mass transport and reaction parameters in bulk media. The technique's ability to obtain the bu...

journal_title：Biophysical journal

authors： Kaufman EN,Jain RK

更新日期：1991-09-01 00:00:00

abstract：:The conformational states of Escherichia coli Rep helicase undergoing ATP hydrolysis while bound to a partial-duplex DNA (pdDNA) were studied using single-molecule FRET. Crystallographic studies showed that Rep bound to single-stranded DNA can exist in open and closed conformations that differ in the orientation of th...

journal_title：Biophysical journal

authors： Balci H,Arslan S,Myong S,Lohman TM,Ha T

更新日期：2011-08-17 00:00:00