Elementary events underlying voltage-dependent G-protein inhibition of N-type calcium channels.


:Voltage-dependent G-protein inhibition of N-type calcium channels reduces presynaptic calcium entry, sharply attenuating neurotransmitter release. Studies in neurons demonstrate that G-proteins have multiple modulatory effects on N-type channels. The observed changes may reflect genuine complexity in G-protein action and/or the intricate interactions of multiple channels and receptors in neurons. Expression of recombinant M2-muscarinic receptors and N-type channels in HEK 293 cells allowed voltage-dependent inhibition to be studied in isolation. In this system, receptor-activated G-proteins had only one effect: a 10-fold increase in the time required for channels to first open following membrane depolarization. There were no changes in gating after the channel first opened, and unitary currents were not detectably altered by modulation. Despite its simplicity, this single change successfully accounts for the complex alterations in whole-cell current observed during G-protein inhibition in neurons.


Biophys J


Biophysical journal


Patil PG,de Leon M,Reed RR,Dubel S,Snutch TP,Yue DT




Has Abstract


1996-11-01 00:00:00














  • NaCl reflection coefficients in proximal tubule apical and basolateral membrane vesicles. Measurement by induced osmosis and solvent drag.

    abstract::Two independent methods, induced osmosis and solvent drag, were used to determine the reflection coefficients for NaCl (sigma NaCl) in brush border and basolateral membrane vesicles isolated from rabbit proximal tubule. In the induced osmosis method, vesicles loaded with sucrose were subjected to varying inward NaCl g...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Pearce D,Verkman AS

    更新日期:1989-06-01 00:00:00

  • Evidence for intermediate S-states as initial phase in the process of oxygen-evolving complex oxidation.

    abstract::We have analyzed flash-induced period-four damped oscillation of oxygen evolution and chlorophyll fluorescence with the aid of a kinetic model of photosystem II. We have shown that, for simulation of the period-four oscillatory behavior of oxygen evolution, it is essential to consider the so-called intermediate S-stat...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Jablonsky J,Lazar D

    更新日期:2008-04-01 00:00:00

  • Interaction of anesthetics with open and closed conformations of a potassium channel studied via molecular dynamics and normal mode analysis.

    abstract::A variety of experiments suggest that membrane proteins are important targets of anesthetic molecules, and that ion channels interact differently with anesthetics in their open and closed conformations. The availability of an open and a closed structural model for the KirBac1.1 potassium channel has made it possible t...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Vemparala S,Domene C,Klein ML

    更新日期:2008-06-01 00:00:00

  • Fluorescence polarization spectroscopy and time-resolved fluorescence kinetics of native cancerous and normal rat kidney tissues.

    abstract::Steady state fluorescence polarization spectra and time-resolved emission decay kinetics have been measured in vitro from malignant and normal rat kidney tissue. The degrees of polarization and emission lifetimes from the cancerous and normal systems are different. The spectroscopic differences are attributed to envir...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Tata DB,Foresti M,Cordero J,Tomashefsky P,Alfano MA,Alfano RR

    更新日期:1986-09-01 00:00:00

  • CW and CCW Conformations of the E. coli Flagellar Motor C-Ring Evaluated by Fluorescence Anisotropy.

    abstract::The molecular cascade that controls switching of the direction of rotation of Escherichia coli flagellar motors is well known, but the conformational changes that allow the rotor to switch are still unclear. The signaling molecule CheY, when phosphorylated, binds to the C-ring at the base of the rotor, raising the pro...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Hosu BG,Berg HC

    更新日期:2018-02-06 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Shanmukh S,Howell P,Baatz JE,Dluhy RA

    更新日期:2002-10-01 00:00:00

  • Gating of cyclic nucleotide-gated (CNGA1) channels by cGMP jumps and depolarizing voltage steps.

    abstract::We expressed rod-type homotetrameric cyclic nucleotide-gated (CNGA1) channels in Xenopus oocytes and studied activation by photolysis-induced jumps of the 3',5'-cyclic guanosine monophosphate (cGMP) concentration and by voltage steps. cGMP jumps to increasing concentrations up to the EC50 value of 46.5 microM decelera...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Nache V,Kusch J,Hagen V,Benndorf K

    更新日期:2006-05-01 00:00:00

  • Site-specific dichroism analysis utilizing transmission FTIR.

    abstract::Infrared spectroscopy has long been used to examine the average secondary structure and orientation of membrane proteins. With the recent utilization of site-specific isotope labeling (e.g., peptidic 1-(13)C = (18)O) it is now possible to examine localized properties, rather than global averages. The technique of site...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Arbely E,Kass I,Arkin IT

    更新日期:2003-10-01 00:00:00

  • Wavelength dependence of cell cloning efficiency after optical trapping.

    abstract::A study on clonal growth in Chinese hamster ovary (CHO) cells was conducted after exposure to optical trapping wavelengths using Nd:YAG (1064 nm) and tunable titanium-sapphire (700-990 nm) laser microbeam optical traps. The nuclei of cells were exposed to optical trapping forces at various wavelengths, power densities...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Liang H,Vu KT,Krishnan P,Trang TC,Shin D,Kimel S,Berns MW

    更新日期:1996-03-01 00:00:00

  • A liquid crystalline phase in spermidine-condensed DNA.

    abstract::Over a large range of salt and spermidine concentrations, short DNA fragments precipitated by spermidine (a polyamine) sediment in a pellet from a dilute isotropic supernatant. We report here that the DNA-condensed phase consists of a cholesteric liquid crystal in equilibrium with a more concentrated phase. These resu...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Sikorav JL,Pelta J,Livolant F

    更新日期:1994-10-01 00:00:00

  • Excitation of the L intermediate of bacteriorhodopsin: electric responses to test x-ray structures.

    abstract::The L intermediate of bacteriorhodopsin was excited, and its electrical response was measured. Two positive components were found in it with respect to the direction of proton pumping: an unresolved fast component, and a slower one (tau=7 micros) of small amplitude. The fast component was assigned to a charge motion c...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Tóth-Boconádi R,Dér A,Taneva SG,Keszthelyi L

    更新日期:2006-04-01 00:00:00

  • Effects of hydration on mechanical properties of a highly sclerotized tissue.

    abstract::The jaws of the bloodworm Glycera dibranchiata consist principally of protein and melanin scaffolds with small amounts of unmineralized copper (Cu) and mineralized atacamite (Cu(2)Cl(OH)(3)) fibers in distinct regions. Remarkably, when tested in air, the regions containing unmineralized Cu are the hardest, stiffest, a...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Moses DN,Pontin MG,Waite JH,Zok FW

    更新日期:2008-04-15 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Boehning D,Joseph SK,Mak DO,Foskett JK

    更新日期:2001-07-01 00:00:00

  • Interruptions in single strands of the DNA in slime mold and other organisms.

    abstract::The molecular weight of single-stranded DNA from the slime mold Physarum polycephalum has been determined by alkaline gradient centrifugation. The average molecular weight during DNA synthesis ( approximately 1.5 x 10(7) D) is less than that observed in nonsynthetic periods ( approximately 4 x 10(7) D). On the basis o...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: McGrath RA,Williams RW

    更新日期:1967-05-01 00:00:00


    abstract::A structural model is suggested for axon membranes consisting of a double layer of lipid and phospholipid molecules in which the polar ends of certain phospholipids change their orientation and combining properties under the influence of an electric field. The phosphate groups act as ion exchange "gates" for the contr...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: GOLDMANN DE

    更新日期:1964-05-01 00:00:00

  • A systematic methodology for defining coarse-grained sites in large biomolecules.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Zhang Z,Lu L,Noid WG,Krishna V,Pfaendtner J,Voth GA

    更新日期:2008-12-01 00:00:00

  • Crystal structure of the Bowman-Birk Inhibitor from Vigna unguiculata seeds in complex with beta-trypsin at 1.55 A resolution and its structural properties in association with proteinases.

    abstract::The structure of the Bowman-Birk inhibitor from Vigna unguiculata seeds (BTCI) in complex with beta-trypsin was solved and refined at 1.55 A to a crystallographic R(factor) of 0.154 and R(free) of 0.169, and represents the highest resolution for a Bowman-Birk inhibitor structure to date. The BTCI-trypsin interface is ...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Barbosa JA,Silva LP,Teles RC,Esteves GF,Azevedo RB,Ventura MM,de Freitas SM

    更新日期:2007-03-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: England JL,Pande VS

    更新日期:2008-10-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Pierdominici-Sottile G,Moffatt L,Palma J

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  • Thermodynamic calculations for biochemical transport and reaction processes in metabolic networks.

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Jol SJ,Kümmel A,Hatzimanikatis V,Beard DA,Heinemann M

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Boal DH

    更新日期:1994-08-01 00:00:00

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    abstract::There is no methodology for the estimation of the dynamic features of large-molecular-weight RNAs in homogeneous physiological media. In this report, a luminescence anisotropy-based method using a long-lifetime luminescent oligonucleotide probe for the estimation of the dynamic features of large-molecular-weight RNA i...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Sakamoto T,Mahara A,Yamagata K,Iwase R,Yamaoka T,Murakami A

    更新日期:2005-12-01 00:00:00

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    abstract::Electron cryomicroscopy of rotor complexes of the Salmonella typhimurium flagellar motor, overproduced in a nonmotile Escherichia coli host, has revealed a variation in subunit symmetry of the cytoplasmic ring (C ring) module. C rings with subunit symmetries ranging from 31 to 38 were found. They formed a Gaussian dis...

    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Young HS,Dang H,Lai Y,DeRosier DJ,Khan S

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  • Fusion Pore Expansion and Contraction during Catecholamine Release from Endocrine Cells.

    abstract::Amperometry recording reveals the exocytosis of catecholamine from individual vesicles as a sequential process, typically beginning slowly with a prespike foot, accelerating sharply to initiate a spike, reaching a peak, and then decaying. This complex sequence reflects the interplay between diffusion, flux through a f...

    journal_title:Biophysical journal

    pub_type: 杂志文章


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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Huet S,Karatekin E,Tran VS,Fanget I,Cribier S,Henry JP

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Vrljic M,Nishimura SY,Brasselet S,Moerner WE,McConnell HM

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Patel DS,Park S,Wu EL,Yeom MS,Widmalm G,Klauda JB,Im W

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Tameyasu T,Ishide N,Pollack GH

    更新日期:1982-02-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Levitan E,Palti Y

    更新日期:1975-03-01 00:00:00

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    journal_title:Biophysical journal

    pub_type: 杂志文章


    authors: Johnson JB,Lamb DC,Frauenfelder H,Müller JD,McMahon B,Nienhaus GU,Young RD

    更新日期:1996-09-01 00:00:00