Abstract:
:Quantitative analysis of DNA microarray data is complicated by uncertainties inherent to the experimental setup. Using computer simulations and real-time experimental results, we have previously demonstrated effects of multiplex reactions on a single sensing zone of an array, which may be a leading factor in erroneous interpretation of experimental data. We suggest here that a simplified three-component kinetic model may present a sufficient approximation to describe the general case of DNA sensing in a complex sample milieu. We show that, by analyzing the real-time hybridization kinetics of a nontarget species, we can perform quantitative analysis of unlabeled targets of interest within a broad dynamic range of concentrations.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Bishop J,Chagovetz AM,Blair Sdoi
10.1529/biophysj.107.121459subject
Has Abstractpub_date
2008-03-01 00:00:00pages
1726-34issue
5eissn
0006-3495issn
1542-0086pii
S0006-3495(08)70611-8journal_volume
94pub_type
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