Interaction of anesthetics with open and closed conformations of a potassium channel studied via molecular dynamics and normal mode analysis.

abstract：:Recent suggestions of nanoscale heat confinement on the surface of synthetic and biogenic magnetic nanoparticles during heating by radio frequency-alternating magnetic fields have generated intense interest because of the potential utility of this phenomenon for noninvasive control of biomolecular and cellular functio...

journal_title：Biophysical journal

authors： Davis HC,Kang S,Lee JH,Shin TH,Putterman H,Cheon J,Shapiro MG

更新日期：2020-03-24 00:00:00

abstract：:The lateral mobility of pyrenyl phospholipid probes in dimyristoylphosphatidylcholine (DMPC) vesicles was determined from the dependence of the pyrene monomeric and excimeric fluorescence yields on the molar probe ratio. The analysis of the experimental data makes use of the milling crowd model for two-dimensional dif...

journal_title：Biophysical journal

authors： Sassaroli M,Vauhkonen M,Perry D,Eisinger J

更新日期：1990-02-01 00:00:00

abstract：:We investigated whether transient step reductions in divalent cations would produce detectable changes in neuronal excitability similar to those reported in the total absence of divalent cations. Using cultured chick dorsal root ganglion cells as a model system, our results indicate that a step reduction in divalent c...

journal_title：Biophysical journal

authors： Hablitz JJ,Heinemann U,Lux HD

更新日期：1986-10-01 00:00:00

abstract：:The conduction properties of ClC-0 and ClC-1 chloride channels are examined using electrostatic calculations and three-dimensional Brownian dynamics simulations. We create an open-state configuration of the prokaryotic ClC Cl(-) channel using its known crystallographic structure as a basis. Two residues that are occlu...

journal_title：Biophysical journal

authors： Corry B,O'Mara M,Chung SH

更新日期：2004-02-01 00:00:00

abstract：:We present a two-component coarse-grained molecular-dynamics model for simulating the erythrocyte membrane. The proposed model possesses the key feature of combing the lipid bilayer and the erythrocyte cytoskeleton, thus showing both the fluidic behavior of the lipid bilayer and the elastic properties of the erythrocy...

journal_title：Biophysical journal

authors： Li H,Lykotrafitis G

更新日期：2012-01-04 00:00:00

abstract：:We describe a new method for selective laser killing of bacteria targeted with light-absorbing gold nanoparticles conjugated with specific antibodies. The multifunctional photothermal (PT) microscope/spectrometer provides a real-time assessment of this new therapeutic intervention. In this integrated system, strong la...

journal_title：Biophysical journal

authors： Zharov VP,Mercer KE,Galitovskaya EN,Smeltzer MS

更新日期：2006-01-15 00:00:00

abstract：:Current far-field fluorescence nanoscopes provide subdiffraction resolution by exploiting a mechanism of fluorescence inhibition. This mechanism is implemented such that features closer than the diffraction limit emit separately when simultaneously exposed to excitation light. A basic mechanism for such transient fluo...

journal_title：Biophysical journal

authors： Testa I,Wurm CA,Medda R,Rothermel E,von Middendorf C,Fölling J,Jakobs S,Schönle A,Hell SW,Eggeling C

更新日期：2010-10-20 00:00:00

abstract：:Theoretical studies of the electronic structure and spectra of models for the ferric resting state and Compound I intermediates of horseradish peroxidase (HRP-I) and catalase (CAT-I) have been performed using the INDO-RHF/CI method. The goals of these studies were twofold: i) to determine whether the axial ligand of H...

journal_title：Biophysical journal

authors： Du P,Loew GH

更新日期：1995-01-01 00:00:00

abstract：:The chromophore of octopus rhodopsin is 11-cis retinal, linked via a protonated Schiff base to the protein backbone. Its stable photoproduct, metarhodopsin, has all-trans retinal as its chromphore. The Schiff base of acid metarhodopsin (lambda max = 510 nm) is protonated, whereas that of alkaline metarhodopsin (lambda...

journal_title：Biophysical journal

authors： Koutalos Y,Ebrey TG,Gilson HR,Honig B

更新日期：1990-08-01 00:00:00

abstract：:A number of membrane proteins act via binding at the water/lipid bilayer interface. An important example of such proteins is provided by the gating-modifier toxins that act on voltage-gated potassium (Kv) channels. They are thought to partition to the headgroup region of lipid bilayers, and so provide a good system fo...

journal_title：Biophysical journal

authors： Wee CL,Gavaghan D,Sansom MS

更新日期：2008-10-01 00:00:00

abstract：:Viral infection begins with the binding of a virus to a specific target on the surface of the host cell, followed by viral genome delivery into the host and a continuation of the infection process. Before binding occurs, the virus must first find its receptor by a process whose details are largely unknown. We applied ...

journal_title：Biophysical journal

authors： Rothenberg E,Sepúlveda LA,Skinner SO,Zeng L,Selvin PR,Golding I

更新日期：2011-06-22 00:00:00

abstract：:The effects of Hofmeister anions on the perpendicular and lateral equation-of-state (EOS) of the dipalmitoylphosphatidylcholine lamellar phase discussed in the companion article are here examined using appropriate free energy models for the intra- and interbilayer interactions. Minimizing the free energy with respect ...

journal_title：Biophysical journal

authors： Leontidis E,Aroti A,Belloni L,Dubois M,Zemb T

更新日期：2007-09-01 00:00:00

abstract：:Membrane proteins diffuse within the membrane, form oligomers and supramolecular assemblies. Using high-speed atomic force microscopy, we present direct experimental measure of an in-membrane-plane interaction potential between membrane proteins. In purple membranes, ATP-synthase c-rings formed dimers that temporarily...

journal_title：Biophysical journal

authors： Casuso I,Sens P,Rico F,Scheuring S

更新日期：2010-10-06 00:00:00

abstract：:We have measured the fluorescence spectra of a single fluorophore attached to a single protein molecule in aqueous solution using a total internal reflection fluorescence microscope. The most reactive cysteine residue of myosin subfragment-1 (S1) was labeled with tetramethylrhodamine. The spectral shift induced by a c...

journal_title：Biophysical journal

authors： Wazawa T,Ishii Y,Funatsu T,Yanagida T

更新日期：2000-03-01 00:00:00

abstract：:Equations are derived describing the dispersion of a permeable solute during Poiseuille flow in a capillary model. It is shown that for the normal range of physiological parameters such as capillary radius, capillary length, blood flow, permeability coefficients, and diffusion constants, the center of mass of a bolus ...

journal_title：Biophysical journal

authors： Pollock F,Blum JJ

更新日期：1966-01-01 00:00:00

abstract：:Ca2+ transport through mitochondrial Ca2+ uniporter is the primary Ca2+ uptake mechanism in respiring mitochondria. Thus, the uniporter plays a key role in regulating mitochondrial Ca2+. Despite the importance of mitochondrial Ca2+ to metabolic regulation and mitochondrial function, and to cell physiology and pathophy...

journal_title：Biophysical journal

authors： Dash RK,Qi F,Beard DA

更新日期：2009-02-18 00:00:00

abstract：:Pavlovian conditioning of the nudibranch mollusc Hermissenda crassicornis was previously shown to produce long-lasting reduction of two K+ currents measured across the Type B photoreceptor soma membrane (Alkon et al., 1982a; Alkon et al., 1985). Pavlovian conditioning of the rabbit was also shown to be followed by per...

journal_title：Biophysical journal

authors： Collin C,Ikeno H,Harrigan JF,Lederhendler I,Alkon DL

更新日期：1988-11-01 00:00:00

abstract：:A cell plated on a two-dimensional substrate forms adhesions with that surface. These adhesions, which consist of aggregates of various proteins, are thought to be important in mechanosensation, the process by which the cell senses and responds to the mechanical properties of the substrate (e.g., stiffness). On the ba...

journal_title：Biophysical journal

authors： Walcott S,Kim DH,Wirtz D,Sun SX

更新日期：2011-12-21 00:00:00

abstract：:Many complex cellular processes from mitosis to cell motility depend on the ability of the cytoskeleton to generate force. Force-generating systems that act on elastic cytoskeletal elements are prone to oscillating instabilities. In this work, we have measured spontaneous shape and movement oscillations in motile fish...

journal_title：Biophysical journal

authors： Barnhart EL,Allen GM,Jülicher F,Theriot JA

更新日期：2010-03-17 00:00:00

abstract：:Recent experiments suggested that cholesterol and other lipid components of high negative spontaneous curvature facilitate membrane fusion. This is taken as evidence supporting the stalk-pore model of membrane fusion in which the lipid bilayers go through intermediate structures of high curvature. How do the high-curv...

journal_title：Biophysical journal

authors： Wang W,Yang L,Huang HW

更新日期：2007-04-15 00:00:00

abstract：:Alamethicin and its analogs from cation selective, multi-conductance channels in lipid bilayers. The conductance levels have been thought to be due to a barrel-stave structure where conducting pores (barrels) are formed by the self-assembly of a variable number of alpha-helical rods (staves). The conductance transitio...

journal_title：Biophysical journal

authors： Laver DR

更新日期：1994-02-01 00:00:00

abstract：:A method for fabricating biomimetic surfaces from intact cell membranes is described. A monolayer of alkanethiol on gold is covered by a second layer derived from the components of erythrocyte membranes either by self-assembly or by Langmuir-Blodgett methods. The resulting asymmetric hybrid layer was characterized by ...

journal_title：Biophysical journal

authors： Rao NM,Plant AL,Silin V,Wight S,Hui SW

更新日期：1997-12-01 00:00:00

abstract：:In diseases of the brain, the distribution and properties of ion channels display deviations from healthy control subjects. We studied three cases of ion channel alteration related to epileptogenesis. The first case of ion channel alteration represents an enhanced sodium current, the second case addresses the downregu...

journal_title：Biophysical journal

authors： Tigerholm J,Fransén E

更新日期：2011-10-19 00:00:00

abstract：:A triple mutant of sperm whale myoglobin (Mb) [Leu(B10) --> Tyr, His(E7) --> Gln, and Thr(E10) --> Arg, called Mb-YQR], investigated by stopped-flow, laser photolysis, crystallography, and molecular dynamics (MD) simulations, proved to be quite unusual. Rebinding of photodissociated NO, O2, and CO from within the prot...

journal_title：Biophysical journal

authors： Brunori M,Cutruzzolà F,Savino C,Travaglini-Allocatelli C,Vallone B,Gibson QH

更新日期：1999-03-01 00:00:00

abstract：:The glycocalyx is a thick coat of proteins and carbohydrates on the outer surface of all eukaryotic cells. Overproduction of large, flexible or rod-like biopolymers, including hyaluronic acid and mucins, in the glycocalyx strongly correlates with the aggression of many cancer types. However, theoretical frameworks to ...

journal_title：Biophysical journal

authors： Gandhi JG,Koch DL,Paszek MJ

更新日期：2019-02-19 00:00:00

abstract：:Large-conductance Ca(2+)-activated K(+) channels (BK) play a fundamental role in modulating membrane potential in many cell types. The gating of BK channels and its modulation by Ca(2+) and voltage has been the subject of intensive research over almost three decades, yielding several of the most complicated kinetic me...

journal_title：Biophysical journal

authors： Rosales RA,Varanda WA

更新日期：2009-05-20 00:00:00

abstract：:Fluorescence resonance energy transfer (FRET) was used to reveal aspects of the mechanism of signal transduction by epidermal growth factor receptors (EGFR). The superpositions of epidermal growth factor (EGF), transforming growth factor-alpha (TGFalpha) and an antibody fragment (29.1) to the carbohydrate extremity of...

journal_title：Biophysical journal

authors： Martin-Fernandez M,Clarke DT,Tobin MJ,Jones SV,Jones GR

更新日期：2002-05-01 00:00:00

abstract：:The dependences of the steady-state critical concentration and average filament length of actin solutions, on the filament branching and capping rates, are calculated using a rate methodology based on the total number of actin filaments. The methodology generalizes calculations of the "treadmilling" actin concentratio...

journal_title：Biophysical journal

authors： Carlsson AE

更新日期：2005-07-01 00:00:00

abstract：:The patch-clamp technique of Mueller (1975, Ann. N.Y. Acad. Sci., 274:247-264) and Neher and Sakmann (1976, Nature (Lond.), 260:799-802) was modified to be suitable for single-channel measurements in lipid bilayers at potentials up to 500 mV. This method was used to study gramicidin A single-channel current-voltage ch...

journal_title：Biophysical journal

authors： Andersen OS

更新日期：1983-02-01 00:00:00

abstract：:A detailed description of the construction and performance of a variable frequency cross-correlation phase fluorometer is reported. The phase fluorometer operates over the frequency range 1-160 MHz with a maximum resolution of a few picoseconds. The effects of distortions introduced by the light modulator and the nonl...

journal_title：Biophysical journal

authors： Gratton E,Limkeman M

更新日期：1983-12-01 00:00:00