Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength.

abstract：:During inflammation, flowing leukocytes tether to and roll on vascular surfaces through the association and dissociation of selectin/ligand bonds. The interactions of P- and L- selectins with their respective ligands exhibit catch-slip bonds, such that increasing force initially prolongs and then shortens bond lifetim...

journal_title：Biophysical journal

authors： Wayman AM,Chen W,McEver RP,Zhu C

更新日期：2010-08-09 00:00:00

abstract：:As sketched in Fig. 1, a current molecular dynamics computer simulation of a lipid bilayer fails to capture significant features of the macroscopic system, including long wavelength undulations. Such fluctuations are intrinsically connected to the value of the macroscopic (or thermodynamic) surface tension (cf. Eqs. 1...

journal_title：Biophysical journal

authors： Feller SE,Pastor RW

更新日期：1996-09-01 00:00:00

abstract：:Studies have been performed on erythrocytes that have been subjected to a low shear stress of less than 100 dyn/cm(2) in a cone-and-plate viscometer. Alterations that were observed included decreased red cell survival, increased osmotic fragility, changes in the cation permeability of the red cell membrane, and a redu...

journal_title：Biophysical journal

authors： Nanjappa BN,Chang HK,Glomski CA

更新日期：1973-11-01 00:00:00

abstract：:Solid phase amplification (SPA), a new method to amplify DNA, is characterized by the use of surface-bound primers. This limits the amplification to two-dimensional surfaces and therefore allows the easy parallelization of DNA amplification in a single system. SPA leads to the formation of small but dense DNA brushes,...

journal_title：Biophysical journal

authors： Mercier JF,Slater GW

更新日期：2005-07-01 00:00:00

abstract：:Formation of low-temperature ordered gel phases in several fully hydrated phosphatidylethanolamines (PEs) and phosphatidylcholines (PCs) with saturated chains as well as in dipalmitoylphosphatidylglycerol (DPPG) was observed by synchrotron x-ray diffraction, microcalorimetry, and densitometry. The diffraction patterns...

journal_title：Biophysical journal

authors： Tenchov B,Koynova R,Rapp G

更新日期：2001-04-01 00:00:00

abstract：:A Brownian dynamics (BD) model described in the accompanying paper (Klenin, K., H. Merlitz, and J. Langowski. 1998. A Brownian dynamics program for the simulation of linear and circular DNA, and other wormlike chain polyelectrolytes. Biophys. J. 74:000-000) has been used for computing the end-to-end distance distribut...

journal_title：Biophysical journal

authors： Merlitz H,Rippe K,Klenin KV,Langowski J

更新日期：1998-02-01 00:00:00

abstract：:The depth of insertion of an antimicrobial peptide, protegrin-1 (PG-1), in lipid bilayers is investigated using solid-state NMR. Paramagnetic Mn(2+) ions bind to the surface of lipid bilayers and induce distance-dependent dipolar relaxation of nuclear spins. By comparing the signal dephasing of the peptide with that o...

journal_title：Biophysical journal

authors： Buffy JJ,Hong T,Yamaguchi S,Waring AJ,Lehrer RI,Hong M

更新日期：2003-10-01 00:00:00

abstract：:Coliphage T4 was used as a model system to study the mechanism of biological inactivation produced by tritium decay. Experimentally, tritiated precursors were incorporated into phage DNA (thymidine-(3)H) or into phage protein ((3)H-amino acids). The ratio of killing efficiencies for decays originating in phage DNA to ...

journal_title：Biophysical journal

authors： Funk F,Person S,Bockrath RC Jr

更新日期：1968-09-01 00:00:00

abstract：:Purple photosynthetic bacteria harvest light using pigment-protein complexes which are often arranged in pseudo-organelles called chromatophores. A model of a chromatophore from Rhodospirillum photometricum was constructed based on atomic force microscopy data. Molecular-dynamics simulations and quantum-dynamics calcu...

journal_title：Biophysical journal

authors： Chandler DE,Strümpfer J,Sener M,Scheuring S,Schulten K

更新日期：2014-06-03 00:00:00

abstract：:Helix-coil transitions in polyalanine molecules of length 10 are studied by multi-canonical Monte Carlo simulations. The solvation effects are included by either a distance-dependent dielectric permittivity or by a term that is proportional to the solvent-accessible surface area of the peptide. We found a strong depen...

journal_title：Biophysical journal

authors： Peng Y,Hansmann UH

更新日期：2002-06-01 00:00:00

abstract：:Fluorescence resonance energy transfer (FRET) was used to reveal aspects of the mechanism of signal transduction by epidermal growth factor receptors (EGFR). The superpositions of epidermal growth factor (EGF), transforming growth factor-alpha (TGFalpha) and an antibody fragment (29.1) to the carbohydrate extremity of...

journal_title：Biophysical journal

authors： Martin-Fernandez M,Clarke DT,Tobin MJ,Jones SV,Jones GR

更新日期：2002-05-01 00:00:00

abstract：:Giant vesicles formed of 1,2-dipalmitoylphosphatidylcholine (DPPC) and sterols (cholesterol or ergosterol) in water and water/ethanol solutions have been used to examine the effect of sterol composition and ethanol concentration on the area compressibility modulus (K(a)), overall mechanical behavior, vesicle morpholog...

journal_title：Biophysical journal

authors： Tierney KJ,Block DE,Longo ML

更新日期：2005-10-01 00:00:00

abstract：:Formation of α-helices is a fundamental process in protein folding and assembly. By studying helix formation in molecular simulations of a series of alanine-based peptides, we obtain the temperature-dependent α-helix propensities of all 20 naturally occurring residues with two recent additive force fields, Amber ff03w...

journal_title：Biophysical journal

authors： Best RB,de Sancho D,Mittal J

更新日期：2012-03-21 00:00:00

abstract：:The equation of motion for sperm flagella, in which the elastic bending moment and the active contractile moment are balanced by the moment from the viscous resistance of the surrounding fluid, is solved for a wave solution that superimposes partial solutions. Substitution of the expression for the wave solution into ...

journal_title：Biophysical journal

authors： Rikmenspoel R

更新日期：1978-08-01 00:00:00

abstract：:Gangliosides are a class of glycosphingolipids (GSLs) with amphiphilic character that are found at the outer leaflet of the cell membranes, where their ability to organize into special domains makes them vital cell membrane components. However, a molecular understanding of GSL-rich membranes in terms of their clustere...

journal_title：Biophysical journal

authors： Patel DS,Park S,Wu EL,Yeom MS,Widmalm G,Klauda JB,Im W

更新日期：2016-11-01 00:00:00

abstract：:We present a theory for proton diffusion through an immobilized protein membrane perfused with an electrolyte and a buffer. Using a Nernst-Planck equation for each species and assuming local charge neutrality, we obtain two coupled nonlinear diffusion equations with new diffusion coefficients dependent on the concentr...

journal_title：Biophysical journal

authors： Zabusky NJ,Deem GS

更新日期：1979-01-01 00:00:00

abstract：:Recent experimental studies (Pusch and Neher, 1988) and theoretical studies (Oliva et al., 1988) have found that the pipette tip is a significant barrier to diffusion in the whole cell patch clamp configuration. In this paper, we extend the theoretical analysis of fluxes between the pipette and cell to include transme...

journal_title：Biophysical journal

authors： Mathias RT,Cohen IS,Oliva C

更新日期：1990-09-01 00:00:00

abstract：:Ion channels assist and control the diffusion of ions through biological membranes. The conduction process depends on the structural characteristics of these nanopores, among which are the hydrophobicity and the afforded diameter of the conduction pathway. In this contribution, we use full atomistic free-energy molecu...

journal_title：Biophysical journal

authors： Treptow W,Tarek M

更新日期：2006-08-01 00:00:00

abstract：:An analysis of four 1-ns molecular dynamics trajectories for two different 15-bp oligonucleotides is presented. Our aim is to show which groups of atoms can be treated as rigid bodies within a bead representation of DNA, independently of the base sequence and for any conformations belonging to the A/B family. Five mod...

journal_title：Biophysical journal

authors： Bruant N,Flatters D,Lavery R,Genest D

更新日期：1999-11-01 00:00:00

abstract：:We measured the influence of alternative versions of the Drosophila melanogaster myosin heavy chain relay domain on muscle mechanical properties. We exchanged relay domain regions (encoded by alternative versions of exon 9) between an embryonic (EMB) isoform and the indirect flight muscle isoform (IFI) of myosin. Prev...

journal_title：Biophysical journal

authors： Yang C,Ramanath S,Kronert WA,Bernstein SI,Maughan DW,Swank DM

更新日期：2008-12-01 00:00:00

abstract：:Calcium channels in neurons mediate a wide variety of essential functions. In addition to contributing to action potential shape, they furnish a substrate that acts as an intracellular second messenger. This study shows that the shape of the neuronal action potential has characteristics that promote long openings of L...

journal_title：Biophysical journal

authors： Mazzanti M,Galli A,Ferroni A

更新日期：1992-10-01 00:00:00

abstract：:Transmissible spongiform encephalopathies are neurodegenerative diseases characterized by the accumulation of an abnormal isoform of the prion protein PrP(Sc). Its fragment 106-126 has been reported to maintain most of the pathological features of PrP(Sc), and a role in neurodegeneration has been proposed based on the...

journal_title：Biophysical journal

authors： Henriques ST,Pattenden LK,Aguilar MI,Castanho MA

更新日期：2008-08-01 00:00:00

abstract：:The early events in halorhodopsin after light excitation are studied with picosecond time resolution. Absorption and fluorescence measurements show that the electronically excited state of the incorporated retinal has a lifetime of 5 ps. Within that time a red-shifted photoproduct is formed that remains stable for at ...

journal_title：Biophysical journal

authors： Polland HJ,Franz MA,Zinth W,Kaiser W,Hegemann P,Oesterhelt D

更新日期：1985-01-01 00:00:00

abstract：:Stochastic effects in gene expression may result in different physiological states of individual cells, with consequences for pathogen survival and artificial gene network design. We studied the contributions of a regulatory factor to gene expression noise in four basic mechanisms of negative gene expression control: ...

journal_title：Biophysical journal

authors： Komorowski M,Miekisz J,Kierzek AM

更新日期：2009-01-01 00:00:00

abstract：:The interaction with lipids of a synthetic peptide corresponding to the transmembrane domain of influenza hemagglutinin was investigated by means of electron spin resonance. A detailed analysis of the electron spin resonance spectra from spin-labeled phospholipids revealed that the major effect of the peptide on the d...

journal_title：Biophysical journal

authors： Ge M,Freed JH

更新日期：2011-01-05 00:00:00

abstract：:Molecular dynamics simulations of protein tyrosine phosphatase 1B (PTP1B) complexed with the phosphorylated peptide substrate DADEpYL and the free substrate have been conducted to investigate 1) the physical forces involved in substrate-protein interactions, 2) the importance of enzyme and substrate flexibility for bi...

journal_title：Biophysical journal

authors： Peters GH,Frimurer TM,Andersen JN,Olsen OH

更新日期：2000-05-01 00:00:00

abstract：:The conformation of ribosomal protein S4 from Escherichia coli has been studied by circular dichroism (CD) and shown to possess unique conformation free in solution. The near ultraviolet spectrum suggests the existence of unique tertiary structural environment for the aromatic amino acid residues. The far ultraviolet ...

journal_title：Biophysical journal

authors： Allen SH,Wong KP

更新日期：1978-09-01 00:00:00

abstract：:The mechanosensitive channel of large conductance (MscL) belongs to a family of transmembrane channel proteins in bacteria and functions as a safety valve that relieves the turgor pressure produced by osmotic downshock. MscL gating can be triggered solely by stretching of the membrane. This work reports an effort to u...

journal_title：Biophysical journal

authors： Jeon J,Voth GA

更新日期：2008-05-01 00:00:00

abstract：:Kinesin-1 is an ATP-driven molecular motor that transports cellular cargo along microtubules. At low loads, kinesin-1 almost always steps forward, toward microtubule plus ends, but at higher loads, it can also step backward. Backsteps are usually 8 nm but can be larger. These larger backward events of 16 nm, 24 nm, or...

journal_title：Biophysical journal

authors： Toleikis A,Carter NJ,Cross RA

更新日期：2020-11-17 00:00:00

abstract：:We have analyzed repellent signal processing in Escherichia coli by flash photorelease of leucine from photolabile precursors. We found that 1). response amplitudes of free-swimming cell populations increased with leucine jump concentration, with an apparent Hill coefficient of 1.3 and a half-maximal dose of 14.4 micr...

journal_title：Biophysical journal

authors： Khan S,Jain S,Reid GP,Trentham DR

更新日期：2004-06-01 00:00:00