Conformational prediction and circular dichroism studies on ribosomal protein S4 from Escherichia coli.

abstract：:The model for carbon monoxide or dioxygen recombination with heme proteins developed by the group at the University of Illinois is reexamined. We propose that the carbon monoxide or dioxygen molecule enters the protein at essentially a diffusion-limited rate determined by the solvent viscosity and that the protein off...

journal_title：Biophysical journal

authors： Austin RH,Chan SS

更新日期：1978-10-01 00:00:00

abstract：:The role of distance-dependent anisotropic reactivity and molecular geometry in the vicinity of localized reaction centers in influencing the rate of bimolecular diffusion-controlled reactions is analyzed in detail, both analytically and numerically. The effect of local molecular shape is considered within the model o...

journal_title：Biophysical journal

authors： Shushin AI,Barzykin AV

更新日期：2001-12-01 00:00:00

abstract：:Thin filament regulation of contraction is thought to involve the binding of two activating ligands: Ca2+ and strongly bound cross-bridges. The specific cross-bridge states required to promote thin filament activation have not been identified. This study examines the relationship between cross-bridge cycling and thin ...

journal_title：Biophysical journal

authors： Wahr PA,Metzger JM

更新日期：1999-04-01 00:00:00

abstract：:Atomistic molecular dynamics simulations are used to probe changes in the nature and subnanosecond dynamical behavior of solvation waters that accompany partial denaturation of the globular protein, human alpha-lactalbumin. A simulated ensemble of subcompact conformers, similar to the molten globule state of human alp...

journal_title：Biophysical journal

authors： Sengupta N,Jaud S,Tobias DJ

更新日期：2008-12-01 00:00:00

abstract：:Cationic antimicrobial cationic peptides (CAMP) have been found in recent years to play a decisive role in hosts' defense against microbial infection. They have also been investigated as a new therapeutic tool, necessary in particular due to the increasing resistance of microbiological populations to antibiotics. The ...

journal_title：Biophysical journal

authors： Howe J,Andrä J,Conde R,Iriarte M,Garidel P,Koch MH,Gutsmann T,Moriyón I,Brandenburg K

更新日期：2007-04-15 00:00:00

abstract：:Based on experimental evidence for 2D array formation of bacteriorhodopsin, we propose a general model for lipid-mediated 2D array formation of membrane proteins in lipid bilayers. The model includes two different lipid species, "annular" lipids and "neutral" lipids, and one protein species. The central assumption of ...

journal_title：Biophysical journal

authors： Sabra MC,Uitdehaag JC,Watts A

更新日期：1998-09-01 00:00:00

abstract：:The arrangement of side chains in alpha-helical regions of protein can be represented by helical net-diagrams. The addition of quantitative indices of the character of the residue at each net-point facilitates a straightforward examination of the groupings of residues of different character. As such the method complem...

journal_title：Biophysical journal

authors： Dunnill P

更新日期：1968-07-01 00:00:00

abstract：:Proteins that cross-link actin filaments can either form bundles of parallel filaments or isotropic networks of individual filaments. We have found that mixtures of actin filaments with alpha-actinin purified from either Acanthamoeba castellanii or chicken smooth muscle can form bundles or isotropic networks depending...

journal_title：Biophysical journal

authors： Wachsstock DH,Schwartz WH,Pollard TD

更新日期：1993-07-01 00:00:00

abstract：:Exchangeable apolipoproteins are located in the surface of lipoprotein particles and regulate lipid metabolism through direct protein-protein and protein-lipid interactions. These proteins are characterized by the presence of tandem repeats of amphiphatic alpha-helix segments and a high surface activity in monolayers ...

journal_title：Biophysical journal

authors： Bolanos-Garcia VM,Renault A,Beaufils S

更新日期：2008-03-01 00:00:00

abstract：:Hundreds of cationic antimicrobial and cell-penetrating peptides (CPPs) form amphipathic α-helices when bound to lipid membranes. Here, we test two hypotheses for the differences in the ability of these peptides to translocate across membranes. The first, which we now call the hydrophobicity hypothesis, is that peptid...

journal_title：Biophysical journal

authors： Ablan FDO,Spaller BL,Abdo KI,Almeida PF

更新日期：2016-10-18 00:00:00

abstract：:Cyclic 3'5' adenosine monophosphate (cAMP)-dependent-protein kinase (PKA) signaling is a fundamental regulatory pathway for mediating cellular responses to hormonal stimuli. The pathway is activated by high-affinity association of cAMP with the regulatory subunit of PKA and signal termination is achieved upon cAMP dis...

journal_title：Biophysical journal

authors： Krishnamurthy S,Moorthy BS,Xin Xiang L,Xin Shan L,Bharatham K,Tulsian NK,Mihalek I,Anand GS

更新日期：2014-09-16 00:00:00

abstract：:The amyloid fibrillar form of the protein Tau is involved in a number of neurodegenerative diseases, also known as tauopathies. In this work, six different fibrillar Tau isoforms were assembled in vitro. The morphological and nanomechanical properties of these isoforms were studied using atomic force microscopy at hig...

journal_title：Biophysical journal

authors： Makky A,Bousset L,Madiona K,Melki R

更新日期：2020-12-15 00:00:00

abstract：:Most of the cellular ATP in living organisms is synthesized by the enzyme F(1)F(o)-ATP synthase. The water soluble F(1) part of the enzyme can also work in reverse and utilize the chemical energy released during ATP hydrolysis to generate mechanical motion. Despite the availability of a large amount of biochemical dat...

journal_title：Biophysical journal

authors： Dittrich M,Hayashi S,Schulten K

更新日期：2003-10-01 00:00:00

abstract：:The wide use of lattice-sum strategies in biomolecular simulations has raised many questions on potential artifacts in these strategies. One interesting question is the artifacts in the counterion distributions of highly charged systems. As one would anticipate, Coulombic interactions under the periodic boundary condi...

journal_title：Biophysical journal

authors： Ye X,Cai Q,Yang W,Luo R

更新日期：2009-07-22 00:00:00

abstract：:De novo formation of cell pairs was used to examine the gating properties of single gap junction channels. Two separate cells of an insect cell line (clone C6/36, derived from the mosquito Aedes albopictus) were pushed against each other to provoke formation of gap junction channels. A dual voltage-clamp method was us...

journal_title：Biophysical journal

authors： Bukauskas FF,Weingart R

更新日期：1994-08-01 00:00:00

abstract：:The motility of Amoeba proteus was examined using the technique of passive particle tracking microrheology, with the aid of newly developed particle tracking software, a fast digital camera, and an optical microscope. We tracked large numbers of endogeneous particles in the amoebae, which displayed subdiffusive motion...

journal_title：Biophysical journal

authors： Rogers SS,Waigh TA,Lu JR

更新日期：2008-04-15 00:00:00

abstract：:The channel-forming properties of two analogs of gramicidin, gramicidin-ethylenediamine (gram-EDA), and gramicidin-N,N-dimethylethylenediamine (gram-DMEDA) were studied in planar lipid bilayers, using protons as the permeant ion. These peptides have positively charged amino groups tethered to their C-terminal ends via...

journal_title：Biophysical journal

authors： Woolley GA,Zunic V,Karanicolas J,Jaikaran AS,Starostin AV

更新日期：1997-11-01 00:00:00

abstract：:Cells in a cloned population of coliform bacteria exhibit a wide range of swimming behaviors--a form of non-genetic individuality. We used computer models to examine the proposition that these variations are due to differences in the number of chemotaxis signaling molecules from one cell to the next. Simulations were ...

journal_title：Biophysical journal

authors： Levin MD,Morton-Firth CJ,Abouhamad WN,Bourret RB,Bray D

更新日期：1998-01-01 00:00:00

abstract：:Phytochromes sense red/far-red light and control many biological processes in plants, fungi, and bacteria. Although the crystal structures of dark- and light-adapted states have been determined, the molecular mechanisms underlying photoactivation remain elusive. Here, we demonstrate that the conserved tongue region of...

journal_title：Biophysical journal

authors： Gustavsson E,Isaksson L,Persson C,Mayzel M,Brath U,Vrhovac L,Ihalainen JA,Karlsson BG,Orekhov V,Westenhoff S

更新日期：2020-01-21 00:00:00

abstract：:We report that voltage-gated Na+ channels (Na(V)) from rat muscle (mu1) expressed in HEK293 cells exhibit anomalous rectification of whole-cell outward current under conditions of symmetrical Na+. This behavior gradually fades with time after membrane break-in, as if a diffusible blocking substance in the cytoplasm is...

journal_title：Biophysical journal

authors： Huang CJ,Moczydlowski E

更新日期：2001-03-01 00:00:00

abstract：:The location of the photopigment molecules relative to the lipid hydrocarbon core of retinal receptor disk membranes was unknown. The photopigment molecules could occur entirely on the surface of the membrane, completely embedded in its hydrocarbon core, or at some intermediate location protruding into both the aqueou...

journal_title：Biophysical journal

authors： Blasie JK

更新日期：1972-02-01 00:00:00

abstract：:We characterized the effect of the first basepair on the conformational dynamics of the fluorescent dye Cy3 attached to the 5' end of double-stranded DNA using gaussian-mixture adaptive umbrella sampling simulations. In the simulations, the sampling of all five dihedral angles along the linker was enhanced, so that bo...

journal_title：Biophysical journal

authors： Spiriti J,Binder JK,Levitus M,van der Vaart A

更新日期：2011-02-16 00:00:00

abstract：:The properties of vesicle membranes prepared from 16:0-SM, 16:0-DHSM, or DPPC were characterized using steady-state and time-resolved fluorescence spectroscopy and different fluorescent reporter molecules. The acyl-chain region was probed using free and phospholipid-bound 1,6-diphenyl-1,3,5-hexatriene. 16:0-DHSM was f...

journal_title：Biophysical journal

authors： Nyholm T,Nylund M,Söderholm A,Slotte JP

更新日期：2003-02-01 00:00:00

abstract：:The molecular code of specific DNA recognition by proteins as a paradigm in molecular biology remains an unsolved puzzle primarily because of the subtle interplay between direct protein-DNA interaction and the indirect contribution from water and ions. Transformation of the nonspecific, low affinity complex to a speci...

journal_title：Biophysical journal

authors： Fuxreiter M,Mezei M,Simon I,Osman R

更新日期：2005-08-01 00:00:00

abstract：:Gastrointestinal slow waves are generated within networks of interstitial cells of Cajal (ICCs). In the intact tissue, slow waves are entrained to neighboring ICCs with higher intrinsic frequencies, leading to active propagation of slow waves. Degradation of ICC networks in humans is associated with motility disorders...

journal_title：Biophysical journal

authors： Du P,O'Grady G,Gibbons SJ,Yassi R,Lees-Green R,Farrugia G,Cheng LK,Pullan AJ

更新日期：2010-05-19 00:00:00

abstract：:Although significant progress has been made toward understanding morphogen-mediated patterning in development, control of the size and shape of tissues via local and global signaling is poorly understood. In particular, little is known about how cell-cell interactions are involved in the control of tissue size. The Hi...

journal_title：Biophysical journal

authors： Gou J,Lin L,Othmer HG

更新日期：2018-08-21 00:00:00

abstract：:Plant light-harvesting complex II (LHCII) is the key antenna complex for plant photosynthesis. We present coarse-grained molecular dynamics simulations of monomeric and trimeric LHCII in a realistic thylakoid membrane environment based on the Martini force field. The coarse-grained protein model has been optimized wit...

journal_title：Biophysical journal

authors： Thallmair S,Vainikka PA,Marrink SJ

更新日期：2019-04-23 00:00:00

abstract：:We have investigated the phase behavior of self-assembled lyotropic liquid crystals (LC) formed by ternary mixtures of oleoylethanolamide (OEA), water and arginine. OEA, a natural analog of the endogenous cannabinoid anandamide involved in the peripheral regulation of feeding, was selected as a main component due to i...

journal_title：Biophysical journal

authors： Mohammady SZ,Pouzot M,Mezzenga R

更新日期：2009-02-18 00:00:00

abstract：:High-efficiency electrofusion between cells of different sizes was achieved by application of fusing electric pulses to cells in centrifuged pellets. Larger target cells (Chinese hamster ovary or L1210 cells) were stacked among smaller human erythrocytes or erythrocyte ghosts by sequential centrifugation at 700 g to f...

journal_title：Biophysical journal

authors： Li LH,Hensen ML,Zhao YL,Hui SW

更新日期：1996-07-01 00:00:00

abstract：:Conditions for which a ligand reversibly bound to a cell surface dissociates and then rebinds to the surface have been theoretically examined. The coupled differential equations that describe reaction at the interface between sites on a plane and three-dimensional solution have been described previously (Thompson, N. ...

journal_title：Biophysical journal

authors： Lagerholm BC,Thompson NL

更新日期：1998-03-01 00:00:00