Abstract:
:The model for carbon monoxide or dioxygen recombination with heme proteins developed by the group at the University of Illinois is reexamined. We propose that the carbon monoxide or dioxygen molecule enters the protein at essentially a diffusion-limited rate determined by the solvent viscosity and that the protein offers no important barriers to this entry. The viscosity dependence of the entry rate k(ED), its magnitude (1 x 10(10) M(-1)s(-1), and the rate of quenching of triplet states of protoprophyrin IX in apomyoglobin by dioxygen are used as supporting evidence. Comparison is made to the model of a fluctuating protein developed by G. Weber.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Austin RH,Chan SSdoi
10.1016/S0006-3495(78)85354-5subject
Has Abstractpub_date
1978-10-01 00:00:00pages
175-86issue
1eissn
0006-3495issn
1542-0086pii
S0006-3495(78)85354-5journal_volume
24pub_type
杂志文章abstract::The membrane-lytic peptide melittin has previously been shown to form pores in lipid bilayers that have been described in terms of two different structural models. In the "barrel stave" model the bilayer remains more or less flat, with the peptides penetrating across the bilayer hydrocarbon region and aggregating to f...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.104.049817
更新日期:2005-03-01 00:00:00
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journal_title:Biophysical journal
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abstract::KL(4) is a 21-residue functional peptide mimic of lung surfactant protein B, an essential protein for lowering surface tension in the alveoli. Its ability to modify lipid properties and restore lung compliance was investigated with circular dichroism, differential scanning calorimetry, and solid-state NMR spectroscopy...
journal_title:Biophysical journal
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doi:10.1016/j.bpj.2008.12.3966
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:1998-05-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.104.041723
更新日期:2004-07-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2014.03.002
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2016-07-26 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2009.05.037
更新日期:2009-08-05 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.107.114363
更新日期:2008-05-15 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2001-10-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(02)75189-8
更新日期:2002-07-01 00:00:00
abstract::The photocycle of salinarum halorhodopsin was investigated in the presence of azide. The azide binds to the halorhodopsin with 150 mM binding constant in the absence of chloride and with 250 mM binding constant in the presence of 1 M chloride. We demonstrate that the azide-binding site is different from that of chlori...
journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2002-04-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2018.05.031
更新日期:2018-07-03 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(03)74595-0
更新日期:2003-09-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2017.03.042
更新日期:2017-06-20 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(00)76778-6
更新日期:2000-05-01 00:00:00
abstract::An alternative theoretical approach to enzyme kinetics that is particularly applicable to single-molecule enzymology is presented. The theory, originated by Van Slyke and Cullen in 1914, develops enzyme kinetics from a "time perspective" rather than the traditional "rate perspective" and emphasizes the nonequilibrium ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.108.131771
更新日期:2008-07-01 00:00:00
abstract::High-affinity and cooperative binding of two Ca(2+) per ATPase (SERCA) occurs within the membrane-bound region of the enzyme. Direct measurements of binding at various Ca(2+) concentrations demonstrate that site-directed mutations within this region interfere selectively with Ca(2+) occupancy of either one or both bin...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(02)75247-8
更新日期:2002-11-01 00:00:00
abstract::The kinetics of J-aggregate formation has been studied for two chromophores, tetrakis-4-sulfonatophenylporphine in an acid medium and pseudoisocyanine on a polyvinylsulfonate template. The assembly processes differ both in their sensitivity to initiation protocols and in the reaction profiles they produce. The porphyr...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(00)76316-8
更新日期:2000-07-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2008.12.3888
更新日期:2009-03-04 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.105.065763
更新日期:2005-11-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2002-01-01 00:00:00
abstract::Eukaryotic genomes are hierarchically organized into protein-DNA assemblies for compaction into the nucleus. Nucleosomes, with the (H3-H4)2 tetrasome as a likely intermediate, are highly dynamic in nature by way of several different mechanisms. We have recently shown that tetrasomes spontaneously change the direction ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2019.07.055
更新日期:2019-12-03 00:00:00
abstract::Uncovering mechanisms that control the dynamics of microtubules is fundamental for our understanding of multiple cellular processes such as chromosome separation and cell motility. Building on previous theoretical work on the dynamic instability of microtubules, we propose here a stochastic model that includes all rel...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2011.12.059
更新日期:2012-03-21 00:00:00
abstract::A microscopic model of passive transverse mass transport of small solutes in the viable epidermal layer of human skin is formulated on the basis of a hexagonal array of cells (i.e., keratinocytes) bounded by 4-nm-thick, anisotropic lipid bilayers and separated by 1-μm layers of extracellular fluid. Gap junctions and t...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2013.03.056
更新日期:2013-05-21 00:00:00
abstract::We report the crystal structure of a bromide-bound form of the D85S mutant of bacteriorhodopsin, bR(D85S), a protein that uses light energy rather than ATP to pump halide ions across the cell membrane. Comparison of the structure of the halide-bound and halide-free states reveals that both displacements of individual ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(03)74490-7
更新日期:2003-07-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2011.02.029
更新日期:2011-04-06 00:00:00
abstract::Unraveling the conformation of membrane-bound viral fusion peptides is essential for understanding how those peptides destabilize the bilayer topology of lipids that is important for virus-cell membrane fusion. Here, molecular dynamics (MD) simulations were performed to investigate the conformation of the 20 amino aci...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.103.024562
更新日期:2004-07-01 00:00:00
abstract::A computational framework is presented for studying the mechanical response of macromolecules. The method combines a continuum mechanics (CM) model for the mechanical properties of the macromolecule with a continuum electrostatic (CE) treatment of solvation. The molecules are represented by their shape and key physico...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2009.01.047
更新日期:2009-05-06 00:00:00
abstract::Reverberating spontaneous synchronized brain activity is believed to play an important role in neural information processing. Whether and how external stimuli can influence this spontaneous activity is poorly understood. Because periodic synchronized network activity is also prominent in in vitro neuronal cultures, we...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.107.112730
更新日期:2008-06-01 00:00:00