Structural significance of the plasma membrane calcium pump oligomerization.

abstract：:The effect of cholesterol on phospholipid acyl chain packing in bilayers consisting of highly unsaturated acyl chains in the liquid crystalline phase was examined for a series of symmetrically and asymmetrically substituted phosphatidylcholines (PCs). The time-resolved fluorescence emission and decay of fluorescence a...

journal_title：Biophysical journal

authors： Mitchell DC,Litman BJ

更新日期：1998-08-01 00:00:00

abstract：:We used high-resolution atomic force microscopy to image the compaction of linear and circular DNA by the yeast mitochondrial protein Abf2p, which plays a major role in packaging mitochondrial DNA. Atomic force microscopy images show that protein binding induces drastic bends in the DNA backbone for both linear and ci...

journal_title：Biophysical journal

authors： Friddle RW,Klare JE,Martin SS,Corzett M,Balhorn R,Baldwin EP,Baskin RJ,Noy A

更新日期：2004-03-01 00:00:00

abstract：:Antisense oligonucleotides present a powerful means to inhibit expression of specific genes, but their effectiveness is limited by factors including cellular delivery, biochemical attack, and poor binding to target. We have developed a systems model of the processes required for an antisense oligonucleotide to enter, ...

journal_title：Biophysical journal

authors： Roth CM

更新日期：2005-10-01 00:00:00

abstract：:We investigated the origins of greater clot rigidity associated with FXIIIa-dependent cross-linking. Fibrin clots were examined in which cross-linking was controlled through the use of two inhibitors: a highly specific active-center-directed synthetic inhibitor of FXIIIa, 1,3-dimethyl-4,5-diphenyl-2[2(oxopropyl)thio]i...

journal_title：Biophysical journal

authors： Ryan EA,Mockros LF,Stern AM,Lorand L

更新日期：1999-11-01 00:00:00

abstract：:The membrane disruption mechanism of pandinin 1 (pin1), an antimicrobial peptide isolated from the venom of the African scorpion, was studied using 31P, 13C, 1H solid-state and multidimensional solution-state NMR spectroscopy. A high-resolution NMR solution structure of pin1 showed that the two distinct alpha-helical ...

journal_title：Biophysical journal

authors： Nomura K,Ferrat G,Nakajima T,Darbon H,Iwashita T,Corzo G

更新日期：2005-12-01 00:00:00

abstract：:The suprachiasmatic nucleus governs daily variations of physiology and behavior in mammals. Within single neurons, interlocked transcriptional/translational feedback loops generate circadian rhythms on the molecular level. We present a mathematical model that reflects the essential features of the mammalian circadian ...

journal_title：Biophysical journal

authors： Becker-Weimann S,Wolf J,Herzel H,Kramer A

更新日期：2004-11-01 00:00:00

abstract：:Differential scanning calorimetry provides a new window into the plasma proteome. Plasma from normal individuals yields a characteristic, reproducible thermogram that appears to represent the weighted sum of denaturation profiles of the most abundant constituent plasma proteins. Plasma from diseased individuals yields...

journal_title：Biophysical journal

authors： Garbett NC,Miller JJ,Jenson AB,Chaires JB

更新日期：2008-02-15 00:00:00

abstract：:We propose mechanisms by which the transmembrane domain of vesicular stomatitis virus (VSV-TMD) promotes both initiation of fusion and formation of a fusion pore. Time courses of polyethyleneglycol (PEG)-mediated fusion of 25 nm small unilamellar vesicles composed of dioleoylphosphatidylcholine, dioleoylphosphatidylet...

journal_title：Biophysical journal

authors： Sengupta T,Chakraborty H,Lentz BR

更新日期：2014-09-16 00:00:00

abstract：:Ion channels and transporters are membrane proteins whose functions are driven by conformational changes. Classical biophysical techniques provide insight into either the structure or the function of these proteins, but a full understanding of their behavior requires a correlation of both these aspects in time. Patch-...

journal_title：Biophysical journal

authors： Kusch J,Zifarelli G

更新日期：2014-03-18 00:00:00

abstract：:Scanning ion conductance microscopy (SICM) is a super-resolution live imaging technique that uses a glass nanopipette as an imaging probe to produce three-dimensional (3D) images of cell surface. SICM can be used to analyze cell morphology at nanoscale, follow membrane dynamics, precisely position an imaging nanopipet...

journal_title：Biophysical journal

authors： Shevchuk A,Tokar S,Gopal S,Sanchez-Alonso JL,Tarasov AI,Vélez-Ortega AC,Chiappini C,Rorsman P,Stevens MM,Gorelik J,Frolenkov GI,Klenerman D,Korchev YE

更新日期：2016-05-24 00:00:00

abstract：:In a contracting muscle, myosin cross-bridges extending from thick filaments pull the interdigitating thin (actin-containing) filaments during cyclical ATP-driven interactions toward the center of the sarcomere, the structural unit of striated muscle. Cross-bridge attachments in the sarcomere have been reported to exh...

journal_title：Biophysical journal

authors： Linari M,Piazzesi G,Pertici I,Dantzig JA,Goldman YE,Lombardi V

更新日期：2020-03-10 00:00:00

abstract：:High-efficiency electrofusion between cells of different sizes was achieved by application of fusing electric pulses to cells in centrifuged pellets. Larger target cells (Chinese hamster ovary or L1210 cells) were stacked among smaller human erythrocytes or erythrocyte ghosts by sequential centrifugation at 700 g to f...

journal_title：Biophysical journal

authors： Li LH,Hensen ML,Zhao YL,Hui SW

更新日期：1996-07-01 00:00:00

abstract：:We have monitored the organization and dynamics of the hemolytic peptide melittin in membranes containing cholesterol by utilizing the intrinsic fluorescence properties of its functionally important sole tryptophan residue and circular dichroism spectroscopy. The significance of this study is based on the fact that th...

journal_title：Biophysical journal

authors： Raghuraman H,Chattopadhyay A

更新日期：2004-10-01 00:00:00

abstract：:The calcium release channels/ryanodine receptors (RyRs) usually form two-dimensional regular lattices in the endoplasmic/sarcoplasmic reticulum membranes. However, the function and modulation of the interaction between neighboring RyRs are still unknown. Here, with an in vitro aqueous system, we demonstrate that the i...

journal_title：Biophysical journal

authors： Hu XF,Liang X,Chen KY,Xie H,Xu Y,Zhu PH,Hu J

更新日期：2005-09-01 00:00:00

abstract：:We have considered whether lecithin surface films below the gel-liquid crystal transition temperature, Tc, are in unique physical states. In general, below Tc, equilibrium films do not exist when surface pressures, pi, exceed about 0.1 dyn/cm. Since surface pressure-surface area isotherms of lecithin films below Tc al...

journal_title：Biophysical journal

authors： Horn LW,Gershfeld NL

更新日期：1977-06-01 00:00:00

abstract：:During postnatal development, major changes in mechanical properties of skeletal muscle occur. We investigated passive properties of skeletal muscle in mice and rabbits that varied in age from 1 day to approximately 1 year. Neonatal skeletal muscle expressed large titin isoforms directly after birth, followed by a gra...

journal_title：Biophysical journal

authors： Ottenheijm CA,Knottnerus AM,Buck D,Luo X,Greer K,Hoying A,Labeit S,Granzier H

更新日期：2009-10-21 00:00:00

abstract：:A major challenge in synthetic gene delivery is to quantitatively predict the optimal design of polymer-based gene carriers (polyplexes). Here, we report a consistent, integrated, and fundamentally grounded computational methodology to address this challenge. This is achieved by accurately representing the spatio-temp...

journal_title：Biophysical journal

authors： Dinh AT,Pangarkar C,Theofanous T,Mitragotri S

更新日期：2007-02-01 00:00:00

abstract：:The 77-K resonance Raman vibrational spectrum of intact goldfish rod photoreceptors containing 3,4-dehydro (A2) retinal is dominated by scattering from the 9-cis component of the steady state at all excitation wavelengths. Intact goldfish photoreceptors were regenerated with an A1-retinal chromophore to determine whet...

journal_title：Biophysical journal

authors： Barry B,Mathies RA,Pardoen JA,Lugtenburg J

更新日期：1987-10-01 00:00:00

abstract：:An equation is derived, which connects two functions P(r) and g(x), the first being related to the scattering intensity by a simple transformation, the second to the elctron density distribution of a spherically symmetric structure. This relation seems to be a suitable starting point for an analysis of shell structure...

journal_title：Biophysical journal

authors： Weick D

更新日期：1974-03-01 00:00:00

abstract：:Prion diseases are infectious fatal neurodegenerative diseases including Creutzfeldt-Jakob disease in humans and bovine spongiform encephalopathy in cattle. The misfolding and conversion of cellular PrP in such mammals into pathogenic PrP is believed to be the key procedure. Rabbits are among the few mammalian species...

journal_title：Biophysical journal

authors： Zhou Z,Yan X,Pan K,Chen J,Xie ZS,Xiao GF,Yang FQ,Liang Y

更新日期：2011-09-21 00:00:00

abstract：:For immobilized nitroxide spin-labels with a well-defined interprobe geometry, resolved dipolar splittings can be observed in continuous wave electron paramagnetic resonance (CW-EPR) spectra for interelectron distances as large as 30 A using perdeuterated probes. In this work, algorithms are developed for calculating ...

journal_title：Biophysical journal

authors： Hustedt EJ,Smirnov AI,Laub CF,Cobb CE,Beth AH

更新日期：1997-04-01 00:00:00

abstract：:In addition to the annular or boundary lipids that surround the transmembrane surface of the potassium channel KcsA from Streptomyces lividans, x-ray crystallographic studies have detected one anionic lipid molecule bound at each protein-protein interface in the homotetrameric structure, at sites referred to as nonann...

journal_title：Biophysical journal

authors： Marius P,Zagnoni M,Sandison ME,East JM,Morgan H,Lee AG

更新日期：2008-03-01 00:00:00

abstract：:Axonal beading, or the formation of a series of swellings along the axon, and retraction are commonly observed shape transformations that precede axonal atrophy in Alzheimer's disease, Parkinson's disease, and other neurodegenerative conditions. The mechanisms driving these morphological transformations are poorly und...

journal_title：Biophysical journal

authors： Datar A,Ameeramja J,Bhat A,Srivastava R,Mishra A,Bernal R,Prost J,Callan-Jones A,Pullarkat PA

更新日期：2019-09-03 00:00:00

abstract：:Two different spin labels, N-(1-oxyl-2,2,5,5-tetramethyl-3-pyrrolidinyl)iodoacetamide (IPSL) and (1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl) methanethiosulfonate (MTSSL), and two different fluorescent labels 5-((((2-iodoacetyl)amino)-ethyl)amino)naphtalene-1-sulfonic acid (IAEDANS) and 6-bromoacetyl-2-dimetylaminon...

journal_title：Biophysical journal

authors： Hammarström P,Owenius R,Mårtensson LG,Carlsson U,Lindgren M

更新日期：2001-06-01 00:00:00

abstract：:Circadian rhythms possess the ability to robustly entrain to the environmental cycles. This ability relies on the phase synchronization of circadian rhythm gene regulation to different environmental cues, of which light is the most obvious and important. The elucidation of the mechanism of circadian entrainment requir...

journal_title：Biophysical journal

authors： Gunawan R,Doyle FJ 3rd

更新日期：2006-09-15 00:00:00

abstract：:The vibrational spectroscopy and population dynamics of excited singlet (2(1)Ag), excited triplet (3B u), and the ground (1Ag) electronic states of carotenoids in chromatophores of Chromatium vinosum (mainly spirilloxanthin and rhodopin) and of the same carotenoids in benzene solutions are examined by picosecond time-...

journal_title：Biophysical journal

authors： Hayashi H,Noguchi T,Tasumi M,Atkinson GH

更新日期：1991-07-01 00:00:00

abstract：:Tethered membranes have been proven during recent years to be a powerful and flexible biomimetic platform. We reported in a previous article on the design of a new architecture based on the self-assembly of a thiolipid on ultrasmooth gold substrates, which shows extremely good electrical sealing properties as well as ...

journal_title：Biophysical journal

authors： Atanasov V,Knorr N,Duran RS,Ingebrandt S,Offenhäusser A,Knoll W,Köper I

更新日期：2005-09-01 00:00:00

abstract：:In two-color fluorescence correlation spectroscopy (TCFCS), the fluorescence intensities of two fluorescently-labeled species are cross-correlated over time and can be used to identify static and dynamic interactions. Generally, fluorophore labels are chosen that do not undergo Förster resonance energy transfer (FRET)...

journal_title：Biophysical journal

authors： Hom EF,Verkman AS

更新日期：2002-07-01 00:00:00

abstract：:Although actin and myosin are important contributors to cell-force generation, shape change, and motility, their contributions to cell stiffness and frequency-dependent rheology have not been conclusively determined. We apply several pharmacological interventions to cultured epithelial cells to elucidate the roles of ...

journal_title：Biophysical journal

authors： Van Citters KM,Hoffman BD,Massiera G,Crocker JC

更新日期：2006-11-15 00:00:00

abstract：:The three-dimensional structure of chromatin affects DNA accessibility and is therefore a key regulator of gene expression. However, the path of the DNA between consecutive nucleosomes, and the resulting chromatin fiber organization remain controversial. The conformational space available for the folding of the nucleo...

journal_title：Biophysical journal

authors： Stehr R,Schöpflin R,Ettig R,Kepper N,Rippe K,Wedemann G

更新日期：2010-03-17 00:00:00