Straightening Out the Elasticity of Myosin Cross-Bridges.

abstract：:The study of mechanotransduction relies on tools that are capable of applying mechanical forces to elicit and assess cellular responses. Here we report a new (to our knowledge) technique, called two-bubble acoustic tweezing cytometry (TB-ATC), for generating spatiotemporally controlled subcellular mechanical forces on...

journal_title：Biophysical journal

authors： Chen D,Sun Y,Gudur MS,Hsiao YS,Wu Z,Fu J,Deng CX

更新日期：2015-01-06 00:00:00

abstract：:The activator protein-1 transcription factor is a heterodimer containing one of each of the Fos and Jun subfamilies of basic-region leucine-zipper proteins. We have previously shown by fluorescence cross-correlation spectroscopy (FCCS) that the fluorescent fusion proteins Fos-EGFP and Jun-mRFP1, cotransfected in HeLa ...

journal_title：Biophysical journal

authors： Vámosi G,Baudendistel N,von der Lieth CW,Szalóki N,Mocsár G,Müller G,Brázda P,Waldeck W,Damjanovich S,Langowski J,Tóth K

更新日期：2008-04-01 00:00:00

abstract：:The effect of agarose on nucleation of hen egg white lysozyme crystal was examined quantitatively using a temperature-jumping technique. For the first time, to our knowledge, the inhibition of agarose during the nucleation of lysozyme was quantified in two respects: a), the effect of increasing interfacial nucleation ...

journal_title：Biophysical journal

authors： Wang L,Liu XY

更新日期：2008-12-15 00:00:00

abstract：:The E5 oncoprotein is the major transforming protein of bovine papillomavirus type 1. This 44-residue transmembrane protein can interact with the platelet-derived growth factor receptor β, leading to ligand-independent activation and cell transformation. For productive interaction, E5 needs to dimerize via a C-termina...

journal_title：Biophysical journal

authors： Windisch D,Hoffmann S,Afonin S,Vollmer S,Benamira S,Langer B,Bürck J,Muhle-Goll C,Ulrich AS

更新日期：2010-09-22 00:00:00

abstract：:During inflammation, flowing leukocytes tether to and roll on vascular surfaces through the association and dissociation of selectin/ligand bonds. The interactions of P- and L- selectins with their respective ligands exhibit catch-slip bonds, such that increasing force initially prolongs and then shortens bond lifetim...

journal_title：Biophysical journal

authors： Wayman AM,Chen W,McEver RP,Zhu C

更新日期：2010-08-09 00:00:00

abstract：:A model for the action of vinblastine (VLB) on cells multiplying exponentially in vivo with a generation time, T(G), has been derived. It is based on the assumption that cells attempting to pass through mitosis in the presence of VLB lose their proliferative capacity and that this lethal effect occurs only when the ce...

journal_title：Biophysical journal

authors： Valeriote FA,Bruce WR,Meeker BE

更新日期：1966-03-01 00:00:00

abstract：:We demonstrate a novel method for stretching a long DNA molecule in agarose gel with alternating current (AC) electric fields. The molecular motion of a long DNA (T4 DNA; 165.6 kb) in agarose gel was studied using fluorescence microscopy. The effects of a wide range of field frequencies, field strengths, and gel conce...

journal_title：Biophysical journal

authors： Kaji N,Ueda M,Baba Y

更新日期：2002-01-01 00:00:00

abstract：:The cytoskeletal protein MreB is an essential component of the bacterial cell-shape generation system. Using a superresolution variant of total internal reflection microscopy with structured illumination, as well as three-dimensional stacks of deconvolved epifluorescence microscopy, we found that inside living Bacillu...

journal_title：Biophysical journal

authors： Olshausen PV,Defeu Soufo HJ,Wicker K,Heintzmann R,Graumann PL,Rohrbach A

更新日期：2013-09-03 00:00:00

abstract：:The effects of chemical modifications of myosin's reactive cysteines on actomyosin adenosine triphosphatase (ATPase) activities and sliding velocities in the in vitro motility assays were examined in this work. The three types of modifications studied were 4-[N-[(iodoacetoxy)ethyl]-N-methylamino]-7-nitrobenz-2-oxa-1,3...

journal_title：Biophysical journal

authors： Root DD,Reisler E

更新日期：1992-09-01 00:00:00

abstract：:The energetics of phospholipid aggregation depend on the apparent water-accessible apolar surface area (ASAap), ordering effects of the chains, and headgroup interactions. We quantify the enthalpy and entropy of these interactions separately. For that purpose, the thermodynamics of micelle formation of lysophosphatidy...

journal_title：Biophysical journal

authors： Heerklotz H,Epand RM

更新日期：2001-01-01 00:00:00

abstract：:A mathematical model is formulated for the development of a population of cells in which the individual members may grow and divide or die. A given cell is characterized by its age and volume, and these parameters are assumed to determine the rate of volume growth and the probability per unit time of division or death...

journal_title：Biophysical journal

authors： Bell GI,Anderson EC

更新日期：1967-07-01 00:00:00

abstract：:This paper describes the use of electrospray ionization-Fourier transform ion cyclotron mass spectrometry (ESI-FTICR-MS) to study the relative stabilities of noncovalent complexes of carbonic anhydrase II (CAII, EC 4.2.1.1) and benzenesulfonamide inhibitors in the gas phase. Sustained off-resonance irradiation collisi...

journal_title：Biophysical journal

authors： Gao J,Wu Q,Carbeck J,Lei QP,Smith RD,Whitesides GM

更新日期：1999-06-01 00:00:00

abstract：:Fluorescence fluctuation spectroscopy has become an important measurement tool for investigating molecular dynamics, molecular interactions, and chemical kinetics in biological systems. Although the basic theory of fluctuation spectroscopy is well established, it is not widely recognized that saturation of the fluores...

journal_title：Biophysical journal

authors： Nagy A,Wu J,Berland KM

更新日期：2005-09-01 00:00:00

abstract：:Crystallographic studies of insulin-protamine complexes, such as neutral protamine Hagedorn (NPH) insulin, have been hampered by high crystal solvent content, small crystal dimensions, and extensive disorder in the protamine molecules. We report herein in situ tapping mode atomic force microscopy (TMAFM) studies of cr...

journal_title：Biophysical journal

authors： Yip CM,Brader ML,Frank BH,DeFelippis MR,Ward MD

更新日期：2000-01-01 00:00:00

abstract：:One of the central tasks in systems biology is to understand how cells regulate their metabolism. Hierarchical regulation analysis is a powerful tool to study this regulation at the metabolic, gene-expression, and signaling levels. It has been widely applied to study steady-state regulation, but analysis of the metabo...

journal_title：Biophysical journal

authors： He F,Stumpf MPH

更新日期：2019-05-21 00:00:00

abstract：:Using glycerinated spasmoneme of giant Zoothamnium sp., the physical properties of spasmoneme before and after Ca2+-induced contraction (pCa 4.5) were investigated. The volume change of spasmoneme contraction was measured under zero tension. The length and diameter decreased by about 50% of their initial value as a re...

journal_title：Biophysical journal

authors： Moriyama Y,Okamoto H,Asai H

更新日期：1999-02-01 00:00:00

abstract：:The recently developed CMAP correction to the CHARMM22 force field (C22) is evaluated from 25 ns molecular dynamics simulations on hen lysozyme. Substantial deviations from experimental backbone root mean-square fluctuations and N-H NMR order parameters obtained in the C22 trajectories (especially in the loops) are el...

journal_title：Biophysical journal

authors： Buck M,Bouguet-Bonnet S,Pastor RW,MacKerell AD Jr

更新日期：2006-02-15 00:00:00

abstract：:The refractive indices, water content, and volume of wet-spun films of Na-hyaluronate have been measured as a function of relative humidity (rh). These data are used with the Lorentz-Lorenz formula to determine the optical polarizabilities of Na-hyaluronate parallel and perpendicular to the helical axis. The analysis ...

journal_title：Biophysical journal

authors： Flowers MR,Marlow RL,Lee SA,Lavalle N,Rupprecht A

更新日期：1992-08-01 00:00:00

abstract：:The effects of Hofmeister anions on the perpendicular and lateral equation-of-state (EOS) of the dipalmitoylphosphatidylcholine lamellar phase discussed in the companion article are here examined using appropriate free energy models for the intra- and interbilayer interactions. Minimizing the free energy with respect ...

journal_title：Biophysical journal

authors： Leontidis E,Aroti A,Belloni L,Dubois M,Zemb T

更新日期：2007-09-01 00:00:00

abstract：:Myoglobin (Mb) is a model system for ligand binding and migration. The energy barriers (ΔG) for ligand migration in Mb have been studied in the past by experiment and theory and significant differences between different approaches were found. From experiment, it is known that Mb can assume a large number of conformati...

journal_title：Biophysical journal

authors： Plattner N,Meuwly M

更新日期：2012-01-18 00:00:00

abstract：:We study the folding mechanism of a triple beta-strand WW domain from the Formin binding protein 28 (FBP28) at atomic resolution with explicit water model using replica exchange molecular dynamics computer simulations. Extended sampling over a wide range of temperatures to obtain the free energy, enthalpy, and entropy...

journal_title：Biophysical journal

authors： Mu Y,Nordenskiöld L,Tam JP

更新日期：2006-06-01 00:00:00

abstract：:We present a combined experimental and computational study of the bundling of F-actin filaments induced by lysozyme proteins. Synchrotron small-angle x-ray scattering results show that these bundles consist of close-packed columnar complexes in which the actin is held together by incommensurate, one-dimensional arrays...

journal_title：Biophysical journal

authors： Guáqueta C,Sanders LK,Wong GC,Luijten E

更新日期：2006-06-15 00:00:00

abstract：:Nanopatterned surfaces are believed to kill bacteria through physical deformation, a mechanism that has immense potential against biochemical resistance. Because of its elusive nature, this mechanism is mostly understood through biophysical modeling. Problematically, accurate descriptions of the contact mechanics and ...

journal_title：Biophysical journal

authors： Velic A,Hasan J,Li Z,Yarlagadda PKDV

更新日期：2021-01-19 00:00:00

abstract：:Supercoiled DNA polymer models for which the torsional energy depends on the total twist of molecules (Tw) are a priori well suited for thermodynamic analysis of long molecules. So far, nevertheless, the exact determination of Tw in these models has been based on a computation of the writhe of the molecules (Wr) by ex...

journal_title：Biophysical journal

authors： Lepage T,Képès F,Junier I

更新日期：2015-07-07 00:00:00

abstract：:Halorhodopsin (HR) and sensory rhodopsin (SR) have been regenerated with retinal analogues that are covalently locked in the 6-s-cis or 6-s-trans conformations. Both pigments regenerate more completely with the locked 6-s-trans retinal and produce analogue pigments with absorption maxima (577 nm for HR and 592 nm for ...

journal_title：Biophysical journal

authors： Baselt DR,Fodor SP,van der Steen R,Lugtenburg J,Bogomolni RA,Mathies RA

更新日期：1989-01-01 00:00:00

abstract：:A number of membrane proteins act via binding at the water/lipid bilayer interface. An important example of such proteins is provided by the gating-modifier toxins that act on voltage-gated potassium (Kv) channels. They are thought to partition to the headgroup region of lipid bilayers, and so provide a good system fo...

journal_title：Biophysical journal

authors： Wee CL,Gavaghan D,Sansom MS

更新日期：2008-10-01 00:00:00

abstract：:Myo1b is a myosin that is exquisitely sensitive to tension. Its actin-attachment lifetime increases > 50-fold when its working stroke is opposed by 1 pN of force. The long attachment lifetime of myo1b under load raises the question: how are actin attachments that last >50 s in the presence of force regulated? Like mos...

journal_title：Biophysical journal

authors： Lewis JH,Greenberg MJ,Laakso JM,Shuman H,Ostap EM

更新日期：2012-06-20 00:00:00

abstract：:We examined the effect of temperature (12-40°C) on local and global Ca2+ signals mediated by inositol trisphosphate receptor/channels (IP3R) in human neuroblastoma (SH-SY5Y) cells. The amplitudes and spatial spread of local signals arising from single IP3R (blips) and clusters of IP3R (puffs) showed little temperature...

journal_title：Biophysical journal

authors： Dickinson GD,Parker I

更新日期：2013-01-22 00:00:00

abstract：:The mechanism of high-voltage pulse-induced permeabilization of the stratum corneum, the outer layer of the skin, is still not completely understood. It has been suggested that joule heating resulting from the applied pulse may play a major role in disrupting the stratum corneum. In this study, electrical and ultrastr...

journal_title：Biophysical journal

authors： Gallo SA,Sen A,Hensen ML,Hui SW

更新日期：2002-01-01 00:00:00

abstract：:Experimental approaches such as fiber diffraction and cryo-electron microscopy reconstruction have defined regulatory positions of tropomyosin on actin but have not, as yet, succeeded at determining key atomic-level contacts between these proteins or fully substantiated the dynamics of their interactions at a structur...

journal_title：Biophysical journal

authors： Pavadai E,Lehman W,Rynkiewicz MJ

更新日期：2020-07-07 00:00:00