Superresolution imaging of dynamic MreB filaments in B. subtilis--a multiple-motor-driven transport?

abstract：:Hybrid global optimization methods attempt to combine the beneficial features of two or more algorithms, and can be powerful methods for solving challenging nonconvex optimization problems. In this paper, novel classes of hybrid global optimization methods, termed alternating hybrids, are introduced for application as...

journal_title：Biophysical journal

authors： Klepeis JL,Pieja MJ,Floudas CA

更新日期：2003-02-01 00:00:00

abstract：:Helicobacter pylori arginase, a bimetallic enzyme, is crucial for pathogenesis of the bacterium in human stomach. Despite conservation of the signature motifs in all arginases, the H. pylori homolog has a non-conserved motif (153ESEEKAWQKLCSL165), whose role was recently shown to be critical for its stability and func...

journal_title：Biophysical journal

authors： George G,Kombrabail M,Raninga N,Sau AK

更新日期：2017-03-28 00:00:00

abstract：:Intracellular recordings of cortical neurons in vivo display intense subthreshold membrane potential (V(m)) activity. The power spectral density of the V(m) displays a power-law structure at high frequencies (>50 Hz) with a slope of approximately -2.5. This type of frequency scaling cannot be accounted for by traditio...

journal_title：Biophysical journal

authors： Bédard C,Destexhe A

更新日期：2008-02-15 00:00:00

abstract：:Spatial patterning of biochemical cues on the micro- and nanometer scale controls numerous cellular processes such as spreading, adhesion, migration, and proliferation. Using force microscopy we show that the lateral spacing of individual integrin receptor-ligand bonds determines the strength of cell adhesion. For spa...

journal_title：Biophysical journal

authors： Selhuber-Unkel C,Erdmann T,López-García M,Kessler H,Schwarz US,Spatz JP

更新日期：2010-02-17 00:00:00

abstract：:A phospholipid chelate complexed with ytterbium (DMPE-DTPA:Yb3+) is shown to be readily incorporated into a model membrane system, which may then be aligned in a magnetic field such that the average bilayer normal lies along the field. This so-called positively ordered smectic phase, whose lipids consist of less than ...

journal_title：Biophysical journal

authors： Prosser RS,Volkov VB,Shiyanovskaya IV

更新日期：1998-11-01 00:00:00

abstract：:The infrared spectra of tripalmitoyl glyceride confirm the tuning fork configuration previously attributed to trilauroyl glyceride (Small, D. M. 1986. Handbook of Lipid Research. Vol. 4). The acyl chains in solid tripalmitoyl glycerol, either within each molecule or between neighboring molecules, are oriented parallel...

journal_title：Biophysical journal

authors： Wong PT,Chagwedera TE,Mantsch HH

更新日期：1989-11-01 00:00:00

abstract：:The separation method, flow field-flow fractionation (flow FFF), is coupled on-line with multiangle laser light scattering (MALLS) for simultaneous measurement of the size and concentration of vesicles eluting continuously from the fractionator. These size and concentration data, gathered as a function of elution time...

journal_title：Biophysical journal

authors： Korgel BA,van Zanten JH,Monbouquette HG

更新日期：1998-06-01 00:00:00

abstract：:The mouse major urinary protein (MUP) has proved to be an intriguing test bed for detailed studies on protein-ligand recognition. NMR, calorimetric, and modeling investigations have revealed that the thermodynamics of ligand binding involve a complex interplay between competing enthalpic and entropic terms. We perform...

journal_title：Biophysical journal

authors： Roy J,Laughton CA

更新日期：2010-07-07 00:00:00

abstract：:A profound remodeling of the extracellular matrix occurs in many epithelial cancers. In ovarian cancer, the minor collagen isoform of Col III becomes upregulated in invasive disease. Here we use second harmonic generation (SHG) imaging microscopy to probe structural differences in fibrillar models of the ovarian strom...

journal_title：Biophysical journal

authors： Tilbury K,Lien CH,Chen SJ,Campagnola PJ

更新日期：2014-01-21 00:00:00

abstract：:More and more researchers are interested in imaging living (Henderson, 1994) or fixed cells in their natural environment using the atomic force microscope (AFM). However, the AFM tip interacts strongly with the sample, and its z range freedom is limited to a few micrometers. This means that the cells to be imaged have...

journal_title：Biophysical journal

authors： Kasas S,Ikai A

更新日期：1995-05-01 00:00:00

abstract：:The B1 domain of protein G has been a classic model system of folding for decades, the subject of numerous experimental and computational studies. Most of the experimental work has focused on whether the protein folds via an intermediate, but the evidence is mostly limited to relatively slow kinetic observations with ...

journal_title：Biophysical journal

authors： Lapidus LJ,Acharya S,Schwantes CR,Wu L,Shukla D,King M,DeCamp SJ,Pande VS

更新日期：2014-08-19 00:00:00

abstract：:Amperometry recording reveals the exocytosis of catecholamine from individual vesicles as a sequential process, typically beginning slowly with a prespike foot, accelerating sharply to initiate a spike, reaching a peak, and then decaying. This complex sequence reflects the interplay between diffusion, flux through a f...

journal_title：Biophysical journal

authors： Jackson MB,Hsiao YT,Chang CW

更新日期：2020-07-07 00:00:00

abstract：:We perform single-molecule spatial tracking measurements of a DNA repair protein, the C-terminal domain of Ada (C-Ada) from Escherichia coli, moving on DNA extended by flow. The trajectories of single proteins labeled with a fluorophore are constructed. We analyze single-protein dwell times on DNA for different flow r...

journal_title：Biophysical journal

authors： Lin Y,Zhao T,Jian X,Farooqui Z,Qu X,He C,Dinner AR,Scherer NF

更新日期：2009-03-04 00:00:00

abstract：:Nipah is an emerging paramyxovirus that is of serious concern to human health. It invades host cells using two of its membrane proteins-G and F. G binds to host ephrins and this stimulates G to activate F. Upon activation, F mediates virus-host membrane fusion. Here we focus on mechanisms that underlie the stimulation...

journal_title：Biophysical journal

authors： Dutta P,Siddiqui A,Botlani M,Varma S

更新日期：2016-10-18 00:00:00

abstract：:Electrically excitable channels were expressed in Chinese hamster ovary cells using a vaccinia virus vector system. In cells expressing rat brain IIA Na+ channels only, brief pulses (< 1 ms) of depolarizing current resulted in action potentials with a prolonged (0.5-3 s) depolarizing plateau; this plateau was caused b...

journal_title：Biophysical journal

authors： Hsu H,Huang E,Yang XC,Karschin A,Labarca C,Figl A,Ho B,Davidson N,Lester HA

更新日期：1993-09-01 00:00:00

abstract：:The geometry of domains in phospholipid bilayers of binary (1:1) mixtures of synthetic lecithins with a difference in chain length of four methylene groups has been studied by two independent, direct and complementary methods. Grazing incidence diffraction of neutrons provided gel domain sizes of less than 10 nm in bo...

journal_title：Biophysical journal

authors： Gliss C,Clausen-Schaumann H,Günther R,Odenbach S,Randl O,Bayerl TM

更新日期：1998-05-01 00:00:00

abstract：:A discrete multistate kinetic model for water-wire proton transport is constructed and analyzed using Monte Carlo simulations. In the model, each water molecule can be in one of three states: oxygen lone-pairs pointing leftward, pointing rightward, or protonated (H(3)O(+)). Specific rules for transitions among these s...

journal_title：Biophysical journal

authors： Chou T

更新日期：2004-05-01 00:00:00

abstract：:Lateral tension in cell plasma membranes plays an essential role in regulation of a number of membrane-related intracellular processes and cell motion. Understanding the physical factors generating the lateral tension and quantitative determination of the tension distribution along the cell membrane is an emerging top...

journal_title：Biophysical journal

authors： Schweitzer Y,Lieber AD,Keren K,Kozlov MM

更新日期：2014-01-07 00:00:00

abstract：:Microorganisms often form complex multicellular assemblies such as biofilms and colonies. Understanding the interplay between assembly expansion, metabolic yield, and nutrient diffusion within a freely growing colony remains a challenge. Most available data on microorganisms are from planktonic cultures, due to the la...

journal_title：Biophysical journal

authors： Vulin C,Di Meglio JM,Lindner AB,Daerr A,Murray A,Hersen P

更新日期：2014-05-20 00:00:00

abstract：:We have worked out a procedure for covalent binding of native biomacromolecules on flat gold surfaces for scanning probe microscopy in aqueous buffer solutions and for other nanotechnological applications, such as the direct measurement of interaction forces between immobilized macromolecules, of their elastomechanica...

journal_title：Biophysical journal

authors： Wagner P,Hegner M,Kernen P,Zaugg F,Semenza G

更新日期：1996-05-01 00:00:00

abstract：:Microtubules are cytoskeletal filaments whose self-assembly occurs by abrupt switching between states of roughly constant growth and shrinkage, a process known as dynamic instability. Understanding the mechanism of dynamic instability offers potential for controlling microtubule-dependent cellular processes such as ne...

journal_title：Biophysical journal

authors： Odde DJ,Cassimeris L,Buettner HM

更新日期：1995-09-01 00:00:00

abstract：:Proteins are denatured in aqueous urea solution. The nature of the molecular driving forces has received substantial attention in the past, whereas the question how urea acts at different phases of unfolding is not yet well understood at the atomic level. In particular, it is unclear whether urea actively attacks fold...

journal_title：Biophysical journal

authors： Stumpe MC,Grubmüller H

更新日期：2009-05-06 00:00:00

abstract：:Myosin subfragment 1 (S1) can be specifically modified at Lys-553 with the fluorescent probe FHS (6-[fluorescein-5(and 6)-carboxamido]hexanoic acid succinimidyl ester) (Bertrand, R., J. Derancourt, and R. Kassab. 1995. Biochemistry. 34:9500-9507), and solvent quenching of FHS-S1 with iodide has been shown to be sensit...

journal_title：Biophysical journal

authors： Cooper WC,Chrin LR,Berger CL

更新日期：2000-03-01 00:00:00

abstract：:By considering how polymer structures are distributed in conformation space, we show that it is possible to quantify the difficulty of structural prediction and to provide a measure of progress for prediction calculations. The critical issue is the probability that a conformation is found within a specified distance o...

journal_title：Biophysical journal

authors： Sullivan DC,Kuntz ID

更新日期：2004-07-01 00:00:00

abstract：:Effects of adducts of [PtCl(NH3)3]Cl or chlorodiethylenetriamineplatinum(II) on DNA stability were studied with emphasis on thermodynamic origins of that stability. Oligodeoxyribonucleotide duplexes (15-bp) containing the single, site-specific monofunctional adduct at G-residues of the central sequences TGT/ACA or 5'-...

journal_title：Biophysical journal

authors： Bursova V,Kasparkova J,Hofr C,Brabec V

更新日期：2005-02-01 00:00:00

abstract：:A simple formula for the expansion rate U of the bilayer membrane perimeter was derived by the solution of the hydrodynamic and the Laplace equations at small rates and small contact angles: U = theta delta/6 micron ln (h/e 3/2 h0), where theta is the equilibrium contact angle between the bilayer membrane and the Plat...

journal_title：Biophysical journal

authors： Dimitrov DS

更新日期：1981-10-01 00:00:00

abstract：:A delayed voltage-dependent K+ current endogenous to Xenopus oocytes has been investigated by the voltage-clamp technique. Both activation and inactivation of the K+ current are voltage-dependent processes. The K+ currents were activated when membrane potential was depolarized from a holding potential of -90 to -50 mV...

journal_title：Biophysical journal

authors： Lu L,Montrose-Rafizadeh C,Hwang TC,Guggino WB

更新日期：1990-06-01 00:00:00

abstract：:We examined the kinetics of voltage-dependent sodium currents in cerebellar Purkinje neurons using whole-cell recording from dissociated neurons. Unlike sodium currents in other cells, recovery from inactivation in Purkinje neurons is accompanied by a sizeable ionic current. Additionally, the extent and speed of recov...

journal_title：Biophysical journal

authors： Raman IM,Bean BP

更新日期：2001-02-01 00:00:00

abstract：:Under various physiological and nonphysiological conditions, the intramembranous particles, as seen by freeze-fracture electron microscopy, may be in various degrees of aggregation. To compare various schemes for the measurement of the degree of aggregation, a computer program has been used to generate simulated aggre...

journal_title：Biophysical journal

authors： Donnell JT 2nd,Finegold LX

更新日期：1981-09-01 00:00:00

abstract：:The pores of ion channel proteins are often modeled as static structures. In this view, selectivity reflects rigidly constrained backbone orientations. Such a picture is at variance with the generalization that biological proteins are flexible, capable of major internal motions on biologically relevant time scales. We...

journal_title：Biophysical journal

authors： Bénitah JP,Ranjan R,Yamagishi T,Janecki M,Tomaselli GF,Marban E

更新日期：1997-08-01 00:00:00