Kinetics of microtubule catastrophe assessed by probabilistic analysis.

abstract：:A new method is presented to measure local cytoplasmic deformation and rate of deformation in motile active neutrophils. The deformation is expressed in terms of biomechanical strains and strain rates. For this purpose small phagocytosed latex microspheres were used as intracellular markers. Planar Lagrangian and Eule...

journal_title：Biophysical journal

authors： Simon SI,Schmid-Schönbein GW

更新日期：1990-08-01 00:00:00

abstract：:A comparative analysis of two models of anisotropic reactivity in bimolecular diffusion-controlled reaction kinetics is presented. One is the conventional model of reactive patches (MRP), where the surface of a molecule is assumed to be reactive over a certain region (circular patch) with the rest of the surface being...

journal_title：Biophysical journal

authors： Barzykin AV,Shushin AI

更新日期：2001-05-01 00:00:00

abstract：:To give a realistic representation of the pulse propagation in arteries a theoretical analysis of the wave propagation through a viscous incompressible fluid contained in an initially stressed elastic tube is considered. The tube is assumed to be orthotropic and its longitudinal motion is constrained by a uniformly di...

journal_title：Biophysical journal

authors： Atabek HB

更新日期：1968-05-01 00:00:00

abstract：:Rates of diffusion through the extracellular space of thin sheets of myocardium from the right ventricular outflow tract of kittens were estimated at 23 degrees C for 45Ca2+ and an inert reference tracer, [14C]sucrose. The myocardial sheets were mounted in an Ussing chamber and equilibrated with Tyrode solution with v...

journal_title：Biophysical journal

authors： Safford RE,Bassingthwaighte JB

更新日期：1977-10-01 00:00:00

abstract：:Structured illumination microscopy is a method that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit by using spatially structured illumination light. Here we describe how this method can be applied in three dimensions to double the axial as well as the lateral resol...

journal_title：Biophysical journal

authors： Gustafsson MG,Shao L,Carlton PM,Wang CJ,Golubovskaya IN,Cande WZ,Agard DA,Sedat JW

更新日期：2008-06-01 00:00:00

abstract：:The adult form of the nicotinic acetylcholine receptor (AChR) consists of five subunits (alpha(2)betaepsilondelta), each having four transmembrane domains (M1-M4). The atomic model of the nicotinic acetylcholine receptor shows that the pore-lining M2 domains make no extensive contacts with the rest of the transmembran...

journal_title：Biophysical journal

authors： Corradi J,Spitzmaul G,De Rosa MJ,Costabel M,Bouzat C

更新日期：2007-01-01 00:00:00

abstract：:The oligomeric structure was determined for four recombinant kinesin derivatives containing N-terminal fragments of the kinesin alpha-subunit. Some of the proteins were dimeric (two-headed) molecules with mechanochemical properties similar to those of intact kinesin. Comparison of the primary and quaternary structures...

journal_title：Biophysical journal

authors： Gelles J,Berliner E,Young EC,Mahtani HK,Perez-Ramirez B,Anderson K

更新日期：1995-04-01 00:00:00

abstract：:Although actin and myosin are important contributors to cell-force generation, shape change, and motility, their contributions to cell stiffness and frequency-dependent rheology have not been conclusively determined. We apply several pharmacological interventions to cultured epithelial cells to elucidate the roles of ...

journal_title：Biophysical journal

authors： Van Citters KM,Hoffman BD,Massiera G,Crocker JC

更新日期：2006-11-15 00:00:00

abstract：:Tethered membranes have been proven during recent years to be a powerful and flexible biomimetic platform. We reported in a previous article on the design of a new architecture based on the self-assembly of a thiolipid on ultrasmooth gold substrates, which shows extremely good electrical sealing properties as well as ...

journal_title：Biophysical journal

authors： Atanasov V,Knorr N,Duran RS,Ingebrandt S,Offenhäusser A,Knoll W,Köper I

更新日期：2005-09-01 00:00:00

abstract：:To understand the thermodynamic exclusion of Na(+) relative to K(+) from the S(2) site of the selectivity filter, the distribution P(X)(epsilon) (X = K(+) or Na(+)) of the binding energy (epsilon) of the ion with the channel is analyzed using the potential distribution theorem. By expressing the excess chemical potent...

journal_title：Biophysical journal

authors： Dixit PD,Merchant S,Asthagiri D

更新日期：2009-03-18 00:00:00

abstract：:Macromolecular transport between the nucleus and cytoplasm occurs through the nuclear pore complexes (NPCs). The NPC in the budding yeast Saccharomyces cerevisiae is a 60-MDa structure embedded in the nuclear envelope and composed of ~30 proteins, termed nucleoporins or nups. Here we present a large-scale analysis of ...

journal_title：Biophysical journal

authors： Damelin M,Silver PA

更新日期：2002-12-01 00:00:00

abstract：:The muscle nicotinic receptor (AChR) is a pentamer of four different subunits, each of which contains four transmembrane domains (M1-M4). We recently showed that channel opening and closing rates of the AChR depend on a hydrogen bond involving a threonine at position 14' of the M4 domain in the alpha-subunit. To deter...

journal_title：Biophysical journal

authors： Bouzat C,Gumilar F,del Carmen Esandi M,Sine SM

更新日期：2002-04-01 00:00:00

abstract：:The development of scanning probe techniques has made it possible to examine protein-protein interactions at the level of individual molecular pairs. A calibrated optical tweezers, along with immunoglobulin G (IgG)-coated polystyrene microspheres, has been used to detect individual surface-linked Staphylococcus protei...

journal_title：Biophysical journal

authors： Stout AL

更新日期：2001-06-01 00:00:00

abstract：:Lipid droplets (LDs) are cytosolic organelles that protrude from the endoplasmic reticulum membrane under energy-rich conditions. How an LD buds off from the endoplasmic reticulum bilayer is still elusive. By using a continuous media description, we computed the morphology of a lipid droplet embedded in between two id...

journal_title：Biophysical journal

authors： Deslandes F,Thiam AR,Forêt L

更新日期：2017-07-11 00:00:00

abstract：:Ras is a membrane-anchored signaling protein that serves as a hub for many signaling pathways and also plays a prominent role in cancer. The intrinsic behavior of Ras on the membrane has captivated the biophysics community in recent years, especially the possibility that it may form dimers. In this article, we describ...

journal_title：Biophysical journal

authors： Chung JK,Lee YK,Denson JP,Gillette WK,Alvarez S,Stephen AG,Groves JT

更新日期：2018-01-09 00:00:00

abstract：:The electronic structure of the circular aggregate of 18 bacteriochlorophyll a (BChl a) molecules responsible for the B850 absorption band of the light-harvesting 2 (LH2) complex of the photosynthetic purple bacterium Rhodopseudomonas acidophila has been studied by measuring fluorescence-excitation spectra of individu...

journal_title：Biophysical journal

authors： Ketelaars M,van Oijen AM,Matsushita M,Köhler J,Schmidt J,Aartsma TJ

更新日期：2001-03-01 00:00:00

abstract：:Fluorescence detection, either involving propagating or near-field emission, is widely being used in spectroscopy, sensing, and microscopy. Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent (near) field, and it is a popular contrast generator for surface-selective fluoresc...

journal_title：Biophysical journal

authors： Oheim M,Salomon A,Brunstein M

更新日期：2020-05-19 00:00:00

abstract：:The bonding rules for actin filament bundles do not lead to a particular packing symmetry, but allow for either regular or disordered filament packing. Indeed, both hexagonal and disordered types of packing are observed in vivo. To investigate factors which control bundle order, as well as size, we examined the effect...

journal_title：Biophysical journal

authors： Stokes DL,DeRosier DJ

更新日期：1991-02-01 00:00:00

abstract：:We analyzed the microstructure in the first-order laser diffraction line from both resting and tetanically contracting single twitch fibers from frog anterior tibial muscle to see if the distribution of sarcomere lengths is continuous or discrete. Measuring the distance between adjacent microstructural elements lying ...

journal_title：Biophysical journal

authors： Tameyasu T,Ishide N,Pollack GH

更新日期：1982-02-01 00:00:00

abstract：:We present MEMLET (MATLAB-enabled maximum-likelihood estimation tool), a simple-to-use and powerful program for utilizing maximum-likelihood estimation (MLE) for parameter estimation from data produced by single-molecule and other biophysical experiments. The program is written in MATLAB and includes a graphical user ...

journal_title：Biophysical journal

authors： Woody MS,Lewis JH,Greenberg MJ,Goldman YE,Ostap EM

更新日期：2016-07-26 00:00:00

abstract：:Single-molecule force spectroscopy is used to probe the kinetics of receptor-ligand bonds by applying mechanical forces to an intermediate media on which the molecules reside. When this intermediate media is a live cell, the viscoelastic properties can affect the calculation of rate constants. We theoretically investi...

journal_title：Biophysical journal

authors： Gupta VK,Neeves KB,Eggleton CD

更新日期：2012-07-03 00:00:00

abstract：:A recurring motif in gene regulatory networks is transcription factors (TFs) that regulate each other and then bind to overlapping sites on DNA, where they interact and synergistically control transcription of a target gene. Here, we suggest that this motif maximizes information flow in a noisy network. Gene expressio...

journal_title：Biophysical journal

authors： Hormoz S

更新日期：2013-03-05 00:00:00

abstract：:Tissues and cells sustain recurring mechanical loads that span a wide range of loading amplitudes and timescales as a consequence of exposure to blood flow, muscle activity, and external impact. Both tissues and cells derive their mechanical strength from fibrous protein scaffolds, which typically have a complex hiera...

journal_title：Biophysical journal

authors： Kurniawan NA,Vos BE,Biebricher A,Wuite GJ,Peterman EJ,Koenderink GH

更新日期：2016-09-06 00:00:00

abstract：:The early events in halorhodopsin after light excitation are studied with picosecond time resolution. Absorption and fluorescence measurements show that the electronically excited state of the incorporated retinal has a lifetime of 5 ps. Within that time a red-shifted photoproduct is formed that remains stable for at ...

journal_title：Biophysical journal

authors： Polland HJ,Franz MA,Zinth W,Kaiser W,Hegemann P,Oesterhelt D

更新日期：1985-01-01 00:00:00

abstract：:Many cell cytoskeletons include an aster of microtubules, with the centrosome serving as the focal point. The position of the centrosome within the cell is important in such directional activities as wound closure and interactions of immune cells. Here we analyzed the centrosome positioning as it is dictated by microt...

journal_title：Biophysical journal

authors： Maly VI,Maly IV

更新日期：2010-11-03 00:00:00

abstract：:The glucocorticoid receptor (GR) is a steroid-hormone-activated transcription factor that modulates gene expression. Transcriptional regulation by the GR requires dynamic receptor binding to specific target sites located across the genome. This binding remodels the chromatin structure to allow interaction with other t...

journal_title：Biophysical journal

authors： Muratcioglu S,Presman DM,Pooley JR,Grøntved L,Hager GL,Nussinov R,Keskin O,Gursoy A

更新日期：2015-09-15 00:00:00

abstract：:Structural rearrangements of the myosin upper-50 kD subdomain are thought to play a key role in coordinating actin binding with nucleotide hydrolysis during the myosin ATPase cycle. Such rearrangements could open and close the active site in opposition to the actin-binding cleft, helping explain the opposing affinitie...

journal_title：Biophysical journal

authors： Robertson CI,Gaffney DP 2nd,Chrin LR,Berger CL

更新日期：2005-09-01 00:00:00

abstract：:The effect of salt and pH titration on the selectivity of spin-labeled analogues of phosphatidic acid, phosphatidylserine, phosphatidylcholine, and stearic acid for the nicotinic acetylcholine receptor (nAcChoR) reconstituted into dioleoylphosphatidylcholine was examined at 0 degrees C using electron spin resonance sp...

journal_title：Biophysical journal

authors： Raines DE,Miller KW

更新日期：1993-03-01 00:00:00

abstract：:In rat mesenteric arteries, smooth muscle cells exhibit intercellular calcium waves in response to local phenylephrine stimulation. These waves have a velocity of approximately 20 cells/s and a range of approximately 80 cells. We analyze these waves in a theoretical model of a population of coupled smooth muscle cells...

journal_title：Biophysical journal

authors： Koenigsberger M,Seppey D,Bény JL,Meister JJ

更新日期：2010-07-21 00:00:00

abstract：:Our ability to understand speech requires neural tuning with high frequency resolution, but the peripheral mechanisms underlying sharp tuning in humans remain unclear. Sharp tuning in genetically modified mice has been attributed to decreases in spread of excitation of tectorial membrane traveling waves. Here we show ...

journal_title：Biophysical journal

authors： Farrahi S,Ghaffari R,Sellon JB,Nakajima HH,Freeman DM

更新日期：2016-09-06 00:00:00