A hydrodynamic theory of bilayer membrane formation.

abstract：:Juxtacrine signaling is an important class of signaling systems that plays a crucial role in various developmental processes ranging from coordination of differentiation between neighboring cells to guiding axon growth during neurogenesis. Such signaling systems rely on the interaction between receptors on one cell an...

journal_title：Biophysical journal

authors： Yaron T,Cordova Y,Sprinzak D

更新日期：2014-11-18 00:00:00

abstract：:Stress fibers are cellular contractile actomyosin machines central to wound healing, shear stress response, and other processes. Contraction mechanisms have been difficult to establish because stress fibers in cultured cells typically exert isometric tension and present little kinetic activity. In a recent study, livi...

journal_title：Biophysical journal

authors： Stachowiak MR,O'Shaughnessy B

更新日期：2009-07-22 00:00:00

abstract：:Endophilins are SH3- and BAR domain-containing proteins implicated in membrane remodeling and vesicle formation. Endophilins A1 and A2 promote the budding of endocytic vesicles from the plasma membrane, whereas endophilin B1 has been implicated in vesicle budding from intracellular organelles, including the trans-Golg...

journal_title：Biophysical journal

authors： Li J,Barylko B,Eichorst JP,Mueller JD,Albanesi JP,Chen Y

更新日期：2016-08-09 00:00:00

abstract：:The cellular cytoplasm is a complex, heterogeneous environment (both spatially and temporally) that exhibits viscoelastic behavior. To further develop our quantitative insight into cellular transport, we analyze data sets of mRNA molecules fluorescently labeled with MS2-GFP tracked in real time in live Escherichia col...

journal_title：Biophysical journal

authors： Lampo TJ,Stylianidou S,Backlund MP,Wiggins PA,Spakowitz AJ

更新日期：2017-02-07 00:00:00

abstract：:Fourier-transform infrared spectroscopy is a method of choice for the experimental determination of protein secondary structure. Numerous approaches have been developed during the past 15 years. A critical parameter that has not been taken into account systematically is the selection of the wavenumbers used for buildi...

journal_title：Biophysical journal

authors： Goormaghtigh E,Ruysschaert JM,Raussens V

更新日期：2006-04-15 00:00:00

abstract：:An algorithm needed for computer simulation of immunodiffusion has been deduced from existing theories of the in vitro reaction between antibody and antigen. The "Goldberg most probable polymer distribution" theory provides a formula that gives the amount of free antibody, free antigen, and diffusible complexes from e...

journal_title：Biophysical journal

authors： Trautman R

更新日期：1973-05-01 00:00:00

abstract：:Potentiometric titrations and surface potential measurements have been used to determine the intrinsic pKa values of both the carboxyl and amino groups of phosphatidylserine (PS) in mixed vesicles of PS and phosphatidylcholine (PC), and also of the amino group of phosphatidylethanolamine (PE) in mixed PE-PC vesicles. ...

journal_title：Biophysical journal

authors： Tsui FC,Ojcius DM,Hubbell WL

更新日期：1986-02-01 00:00:00

abstract：:Trimethylamine-N-oxide (TMAO) in the cells of sharks and rays is believed to counteract the deleterious effects of the high intracellular concentrations of urea in these animals. It has been hypothesized that TMAO has the generic ability to counteract the effects of urea on protein structure and function, regardless o...

journal_title：Biophysical journal

authors： Baskakov I,Wang A,Bolen DW

更新日期：1998-05-01 00:00:00

abstract：:It is often assumed that pH(i) is spatially uniform within cells. A double-barreled microperfusion system was used to apply solutions of weak acid (acetic acid, CO(2)) or base (ammonia) to localized regions of an isolated ventricular myocyte (guinea pig). A stable, longitudinal pH(i) gradient (up to 1 pH(i) unit) was ...

journal_title：Biophysical journal

authors： Swietach P,Leem CH,Spitzer KW,Vaughan-Jones RD

更新日期：2005-04-01 00:00:00

abstract：:Small amounts of paramagnetic cations quench the phosphorescence of DNA. Although the emission intensity is monotonic with increasing thymine content, the quenching efficiency is not. The cations quench from phosphate sites. ...

journal_title：Biophysical journal

authors： Isenberg I,Rosenbluth R,Baird SL Jr

更新日期：1967-07-01 00:00:00

abstract：:Bases, nucleosides, nucleotides, and polynucleotides were exposed to chemically generated singlet oxygen to determine whether the species oxidized paralleled those oxidized in photodynamic reactions. In neutral or basic aqueous solution guanine, guanosine, deoxyguanosine, guanylic acid, deoxyguanylic acid, thymine, an...

journal_title：Biophysical journal

authors： Hallett FR,Hallett BP,Snipes W

更新日期：1970-04-01 00:00:00

abstract：:We derive the statistics of the signals generated by shape fluctuations of large molecules studied by feedback tracking microscopy. We account for the influence of intramolecular dynamics on the response of the tracking system and derive a general expression for the fluorescence autocorrelation function that applies w...

journal_title：Biophysical journal

authors： McHale K,Mabuchi H

更新日期：2010-07-07 00:00:00

abstract：:Actin filaments are semiflexible polymers that display large-scale conformational twisting and bending motions. Modulation of filament bending and twisting dynamics has been linked to regulatory actin-binding protein function, filament assembly and fragmentation, and overall cell motility. The relationship between act...

journal_title：Biophysical journal

authors： De La Cruz EM,Roland J,McCullough BR,Blanchoin L,Martiel JL

更新日期：2010-09-22 00:00:00

abstract：:The adhesion of giant unilamellar phospholipid vesicles to planar substrates coated with extracellular matrix mimetic cushions of hyaluronan is studied using quantitative reflection interference contrast microscopy. The absolute height of the vesicle membrane at the vicinity of the substrate is measured by considering...

journal_title：Biophysical journal

authors： Limozin L,Sengupta K

更新日期：2007-11-01 00:00:00

abstract：:The B1 domain of protein G has been a classic model system of folding for decades, the subject of numerous experimental and computational studies. Most of the experimental work has focused on whether the protein folds via an intermediate, but the evidence is mostly limited to relatively slow kinetic observations with ...

journal_title：Biophysical journal

authors： Lapidus LJ,Acharya S,Schwantes CR,Wu L,Shukla D,King M,DeCamp SJ,Pande VS

更新日期：2014-08-19 00:00:00

abstract：:Calculations are reported of the time-dependent Nernst-Planck equations for a thin permeable membrane between electrolytic solutions. Charge neutrality is assumed for the time-dependent case. The response of such a membrane system to step current input is measured in terms of the time and space changes in concentratio...

journal_title：Biophysical journal

authors： COHEN H,COOLEY JW

更新日期：1965-03-01 00:00:00

abstract：:Advances in biotechnology and computer science are providing the possibility to construct mathematical models for complex biological networks and systematically understand their properties. Traditional network identification approaches, however, cannot accurately recover the model parameters from the noisy laboratory ...

journal_title：Biophysical journal

authors： Feng XJ,Rabitz H

更新日期：2004-03-01 00:00:00

abstract：:Integrin-mediated adhesion of circulating neutrophils to endothelium during inflammation involves multiple adhesion molecules on both neutrophils and endothelium. Most studies of neutrophil adhesion have focused on adhesion to ICAM-1 (mediated by beta(2) integrins), but interaction with the endothelial ligand vascular...

journal_title：Biophysical journal

authors： Lomakina EB,Waugh RE

更新日期：2009-01-01 00:00:00

abstract：:We explore the means by which immobilization of a substrate on a surface can increase the rate of a diffusion-controlled enzymatic reaction. A quasichemical approach is developed and compared with Brownian dynamics simulations. We use these methods to show that restricting only the orientation of the enzyme by long-ra...

journal_title：Biophysical journal

authors： Nag A,Dinner AR

更新日期：2006-02-01 00:00:00

abstract：:Recent development of titratable coions has paved the way for realizing all-atom molecular dynamics at constant pH. To further improve physical realism, here we describe a technique in which proton titration of the solute is directly coupled to the interconversion between water and hydroxide or hydronium. We test the ...

journal_title：Biophysical journal

authors： Chen W,Wallace JA,Yue Z,Shen JK

更新日期：2013-08-20 00:00:00

abstract：:Skinned fibers of frog semitendinosus muscle could easily be stretched up to 8 mum or more in sarcomere length. Such extremely stretched fibers gave quite sharp optical diffraction patterns. The intensities of all observable diffraction lines were found to increase on application of electric field (10 similar to 100 V...

journal_title：Biophysical journal

authors： Umazume Y,Fujime S

更新日期：1975-02-01 00:00:00

abstract：:Several hundred solid state NMR dipolar couplings and chemical shift anisotropies were simulated for the polytopic membrane protein, bacteriorhodopsin, and for an idealized transmembrane peptide conforming to several different secondary structures (alpha- and 3(10)-helices and parallel and antiparallel beta-sheets), e...

journal_title：Biophysical journal

authors： Sanders CR 2nd,Schwonek JP

更新日期：1993-10-01 00:00:00

abstract：:PSII catalyzes the oxidation of water and reduction of plastoquinone in oxygenic photosynthesis. PSII contains an oxygen-evolving complex, which is located on the lumenal side of the PSII reaction center and which contains manganese, calcium, and chloride. Four sequential photooxidation reactions are required to gener...

journal_title：Biophysical journal

authors： De Riso A,Jenson DL,Barry BA

更新日期：2006-09-01 00:00:00

abstract：:A two-dimensional (2D) model of lipid bilayers was developed and used to investigate a possible role of membrane lateral tension in membrane fusion. We found that an increase of lateral tension in contacting monolayers of 2D analogs of liposomes and planar membranes could cause not only hemifusion, but also complete f...

journal_title：Biophysical journal

authors： Chanturiya A,Scaria P,Kuksenok O,Woodle MC

更新日期：2002-06-01 00:00:00

abstract：:The ability to discriminate between different cations efficiently is essential for the proper physiological functioning of many membrane transport proteins. One obvious mechanism of ion selectivity is when a binding site is structurally constrained by the protein architecture and its geometry is precisely adapted to f...

journal_title：Biophysical journal

authors： Roux B

更新日期：2010-06-16 00:00:00

abstract：:Mechanosensitivity in voltage-gated calcium channels could be an asset to calcium signaling in healthy cells or a liability during trauma. Recombinant N-type channels expressed in HEK cells revealed a spectrum of mechano-responses. When hydrostatic pressure inflated cells under whole-cell clamp, capacitance was unchan...

journal_title：Biophysical journal

authors： Calabrese B,Tabarean IV,Juranka P,Morris CE

更新日期：2002-11-01 00:00:00

abstract：:The results of variable dielectric coefficient Poisson-Boltzmann calculations of the counter-ion concentration in the vicinity of an all-atom model of the B-form of DNA are presented with an emphasis on the importance of spatial variations in the dielectric properties of the solvent, particularly at the macro-ion-solv...

journal_title：Biophysical journal

authors： Pack GR,Garrett GA,Wong L,Lamm G

更新日期：1993-10-01 00:00:00

abstract：:Recent studies have indicated that the tip links and kinocilial links of sensory hair bundles in the inner ear have similar properties and share a common epitope, and that cadherin 23 may also be a component of each link type. Transmission electron microscopy was therefore used to study and compare the fine structure ...

journal_title：Biophysical journal

authors： Tsuprun V,Goodyear RJ,Richardson GP

更新日期：2004-12-01 00:00:00

abstract：:In addition to being the gateway for all access to the eukaryotic genome, chromatin has in recent years been identified as carrying an epigenetic code regulating transcriptional activity. Though much is known about the biochemistry of this code, little is understood regarding the different fiber structures through whi...

journal_title：Biophysical journal

authors： Depken M,Schiessel H

更新日期：2009-02-01 00:00:00

abstract：:Acyl chain dynamics of the trans-unsaturated lipid, dielaidoylphosphatidylcholine (DEPC), were studied by conventional and saturation transfer electron paramagnetic resonance spectroscopy of aqueous dispersions of DEPC spin labeled with lecithins having doxyl groups at positions 5, 10, and 14 on the sn-2 chain. The ge...

journal_title：Biophysical journal

authors： Dalton LA,Miller KW

更新日期：1993-10-01 00:00:00