The intrinsic pKa values for phosphatidylserine and phosphatidylethanolamine in phosphatidylcholine host bilayers.

Abstract:

:Potentiometric titrations and surface potential measurements have been used to determine the intrinsic pKa values of both the carboxyl and amino groups of phosphatidylserine (PS) in mixed vesicles of PS and phosphatidylcholine (PC), and also of the amino group of phosphatidylethanolamine (PE) in mixed PE-PC vesicles. The pKa of the carboxyl group of PS in liposomes with different PS/PC lipid ratios measured by the two different methods is 3.6 +/- 0.1, and the pKa of its amino group is 9.8 +/- 0.1. The pKa of the amino group of PE in PE-PC vesicles, determined solely by surface potential measurements, is 9.6 +/- 0.1. These pKa values are independent of the aqueous phase ionic strength and of the effect of the liposome's surface potential due to the presence of these partially charged lipids.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Tsui FC,Ojcius DM,Hubbell WL

doi

10.1016/S0006-3495(86)83655-4

subject

Has Abstract

pub_date

1986-02-01 00:00:00

pages

459-68

issue

2

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(86)83655-4

journal_volume

49

pub_type

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