The structure of tip links and kinocilial links in avian sensory hair bundles.

abstract：:In adherent and motile neutrophils NAD(P)H concentration, flavoprotein redox potential, and production of reactive oxygen species and nitric oxide, are all periodic and exhibit defined phase relationships to an underlying metabolic oscillation of approximately 20 s. Utilizing fluorescence microscopy, we have shown in ...

journal_title：Biophysical journal

authors： Rosenspire AJ,Kindzelskii AL,Simon BJ,Petty HR

更新日期：2005-05-01 00:00:00

abstract：:Reversible blockage of the voltage-dependent anion channel (VDAC) of the mitochondrial outer membrane by dimeric tubulin is being recognized as a potent regulator of mitochondrial respiration. The tubulin-blocked state of VDAC is impermeant for ATP but only partially closed for small ions. This residual conductance al...

journal_title：Biophysical journal

authors： Gurnev PA,Queralt-Martin M,Aguilella VM,Rostovtseva TK,Bezrukov SM

更新日期：2012-05-02 00:00:00

abstract：:Flow dialysis has found widespread use in determining the dissociation constant (KD) of a protein-ligand interaction or the amount of available binding sites (E0). This method has the potency to measure both these parameters in a single experiment and in this article a method to measure simultaneously the KD and E0 is...

journal_title：Biophysical journal

authors： Veldhuis G,Vos EP,Broos J,Poolman B,Scheek RM

更新日期：2004-04-01 00:00:00

abstract：:For proteins of known structure, the relative enthalpic stability with respect to wild-type, ΔΔH(U), can be estimated by direct computation of the folded and unfolded state energies. We propose a model by which the change in stability upon mutation can be predicted from all-atom molecular dynamics simulations for the ...

journal_title：Biophysical journal

authors： Dadarlat VM,Gorenstein LA,Post CB

更新日期：2012-10-17 00:00:00

abstract：:The intrinsic birefringences of TMV, tropocollagen, and paramyosin were calculated from flow birefringence measurements using the theory of Peterlin and Stuart. The values are -0.029, -0.029, and -0.030, respectively. The intrinsic birefringences of TMV and tropocollagen were measured as a function of the refractive i...

journal_title：Biophysical journal

authors： TAYLOR EW,CRAMER W

更新日期：1963-03-01 00:00:00

abstract：:The Jun a 3 protein from mountain cedar (Juniperus ashei) pollen, a member of group 5 of the family of plant pathogenesis-related proteins (PR-proteins), reacts with serum IgE from patients with cedar hypersensitivity. We used the crystal structures of two other proteins of this group, thaumatin and an antifungal prot...

journal_title：Biophysical journal

authors： Soman KV,Midoro-Horiuti T,Ferreon JC,Goldblum RM,Brooks EG,Kurosky A,Braun W,Schein CH

更新日期：2000-09-01 00:00:00

abstract：:The structure and dynamics of a large segment of Ste2p, the G-protein-coupled alpha-factor receptor from yeast, were studied in dodecylphosphocholine (DPC) micelles using solution NMR spectroscopy. We investigated the 73-residue peptide EL3-TM7-CT40 consisting of the third extracellular loop 3 (EL3), the seventh trans...

journal_title：Biophysical journal

authors： Neumoin A,Arshava B,Becker J,Zerbe O,Naider F

更新日期：2007-07-15 00:00:00

abstract：:Spectroscopic responses of the potentiometric probe 2-(4-(dimethylamino)styryl)-1-methylpyridinium iodide (DASPMI) were investigated in living cells by means of a time- and space-correlated single photon counting technique. Spatially resolved fluorescence decays from single mitochondria or only a very few organelles o...

journal_title：Biophysical journal

authors： Ramadass R,Bereiter-Hahn J

更新日期：2008-10-01 00:00:00

abstract：:Noncanonical DNA structures that retain programmability and structural predictability are increasingly being used in DNA nanotechnology applications, in which they offer versatility beyond traditional Watson-Crick interactions. The d(CGA) triplet repeat motif is structurally dynamic and can transition between parallel...

journal_title：Biophysical journal

authors： Luteran EM,Kahn JD,Paukstelis PJ

更新日期：2020-10-20 00:00:00

abstract：:Branched cable voltage recording and voltage clamp analytical solutions derived in two previous papers are used to explore practical issues concerning voltage clamp. Single exponentials can be fitted reasonably well to the decay phase of clamped synaptic currents, although they contain many underlying components. The ...

journal_title：Biophysical journal

authors： Major G

更新日期：1993-07-01 00:00:00

abstract：:Photoinduced electron transfer in photosystem I (PS I) proceeds from the excited primary electron donor P700 (a chlorophyll a dimer) via the primary acceptor A0 (chlorophyll a) and the secondary acceptor A1 (phylloquinone) to three [4Fe-4S] clusters, Fx, FA, and FB. Prereduction of the iron-sulfur clusters blocks elec...

journal_title：Biophysical journal

authors： Polm M,Brettel K

更新日期：1998-06-01 00:00:00

abstract：:The effects of the anionic tungsten carbonyl complex [W(CO)(5)SC(6)H(5)](-) and its fluorinated analog [W(CO)(5)SC(6)F(5)](-) on the electrical properties of the plasma membrane of mouse myeloma cells were studied by the single-cell electrorotation technique. At micromolar concentrations, both compounds gave rise to a...

journal_title：Biophysical journal

authors： Kürschner M,Nielsen K,von Langen JR,Schenk WA,Zimmermann U,Sukhorukov VL

更新日期：2000-09-01 00:00:00

abstract：:In this review, we focus on the early events in the process of fibroblast spreading on fibronectin matrices of different rigidities. We present a focused position piece that illustrates the many different tests that a cell makes of its environment before it establishes mature matrix adhesions. When a fibroblast is pla...

journal_title：Biophysical journal

authors： Wolfenson H,Iskratsch T,Sheetz MP

更新日期：2014-12-02 00:00:00

abstract：:The redox-driven proton pump cytochrome c oxidase is that enzymatic machinery of the respiratory chain that transfers electrons from cytochrome c to molecular oxygen and thereby splits molecular oxygen to form water. To investigate the reaction mechanism of cytochrome c oxidase on the single vibrational level, we used...

journal_title：Biophysical journal

authors： Heitbrink D,Sigurdson H,Bolwien C,Brzezinski P,Heberle J

更新日期：2002-01-01 00:00:00

abstract：:mRNAs are involved in complicated supramolecular complexes with human 40S and 80S ribosomes responsible for the protein synthesis. In this work, a derivative of nonaribonucleotide pUUCGUAAAA with nitroxide spin labels attached to the 5'-phosphate and to the C8 atom of the adenosine in sixth position (mRNA analog) was ...

journal_title：Biophysical journal

authors： Malygin AA,Graifer DM,Meschaninova MI,Venyaminova AG,Krumkacheva OA,Fedin MV,Karpova GG,Bagryanskaya EG

更新日期：2015-12-15 00:00:00

abstract：:In wild-type lenses from various species, an intracellular hydrostatic pressure gradient goes from ∼340 mmHg in central fiber cells to 0 mmHg in surface cells. This gradient drives a center-to-surface flow of intracellular fluid. In lenses in which gap-junction coupling is increased, the central pressure is lower, whe...

journal_title：Biophysical journal

authors： Gao J,Sun X,White TW,Delamere NA,Mathias RT

更新日期：2015-11-03 00:00:00

abstract：:The rate of muscle force redevelopment after release-restretch protocols has previously been interpreted using a simple two-state cross-bridge cycling model with rate constants for transitions between non-force-bearing and force-bearing states, f, and between force-bearing and non-force-bearing states, g. Changes in t...

journal_title：Biophysical journal

authors： Campbell K

更新日期：1997-01-01 00:00:00

abstract：:We propose a mechanism for the formation of membrane oscillations and traveling waves, which arise due to the coupling between the actin cytoskeleton and the calcium flux through the membrane. In our model, the fluid cell membrane has a mobile but constant population of proteins with a convex spontaneous curvature, wh...

journal_title：Biophysical journal

authors： Veksler A,Gov NS

更新日期：2009-09-16 00:00:00

abstract：:Large and rapid changes in light scattering accompany secretion from nerve terminals of the mammalian neurohypophysis (posterior pituitary). In the mouse, these intrinsic optical signals are intimately related to the arrival of the action potential E-wave and the release of arginine vasopressin and oxytocin (S-wave). ...

journal_title：Biophysical journal

authors： Kim GH,Kosterin P,Obaid AL,Salzberg BM

更新日期：2007-05-01 00:00:00

abstract：:The determination of intracellular protein concentrations is a prerequisite for understanding protein interaction networks in systems biology. Today, protein quantification is based either on mass spectrometry, which requires large cell numbers and sophisticated measurement protocols, or on quantitative Western blotti...

journal_title：Biophysical journal

authors： Chung YD,Sinzinger MD,Bovee-Geurts P,Krause M,Dinkla S,Joosten I,Koopman WJ,Adjobo-Hermans MJ,Brock R

更新日期：2011-12-07 00:00:00

abstract：:The ability to discriminate between different cations efficiently is essential for the proper physiological functioning of many membrane transport proteins. One obvious mechanism of ion selectivity is when a binding site is structurally constrained by the protein architecture and its geometry is precisely adapted to f...

journal_title：Biophysical journal

authors： Roux B

更新日期：2010-06-16 00:00:00

abstract：:The pore-forming alpha 1 subunit of L-type calcium (Ca2+) channels is the molecular target of Ca2+ channel blockers such as phenylalkylamines (PAAs). Association and dissociation rates of (-)devapamil were compared for a highly PAA-sensitive L-type Ca2+ channel chimera (Lh) and various class A Ca2+ channel mutants. Th...

journal_title：Biophysical journal

authors： Degtiar VE,Aczél S,Döring F,Timin EN,Berjukow S,Kimball D,Mitterdorfer J,Hering S

更新日期：1997-07-01 00:00:00

abstract：:Aerotaxis is a particular form of "energy taxis". It is based on a largely elusive signal transduction machinery. In aerotaxis, oxygen dissolved in water plays the role of both attractant (at moderate concentrations) and repellent (at high and low concentrations). Cells swimming from favorable oxygen concentrations in...

journal_title：Biophysical journal

authors： Mazzag BC,Zhulin IB,Mogilner A

更新日期：2003-12-01 00:00:00

abstract：:The structure of crotapotin, a protein extracted, from the venom of the Crotalus durissus terrificus, in solution at pH = 1.5, was studied by SAXS. The experimental results yield structural parameter values of the molecular radius of gyration Rg = 13.6 A, volume v = 16.2 x 10(3) A3 A3 and maximal dimension Dmax = 46 A...

journal_title：Biophysical journal

authors： Abrego JR,Craievich AF,Mascarenhas YP,Laure CJ

更新日期：1993-02-01 00:00:00

abstract：:The possibility that the chlorophyll (chl) ring distortions observed in the crystal structures of chl-protein complexes are involved in the transition energy modulation, giving rise to the spectral forms, is investigated. The out-of-plane chl-macrocycle distortions are described using an orthonormal set of deformation...

journal_title：Biophysical journal

authors： Zucchelli G,Brogioli D,Casazza AP,Garlaschi FM,Jennings RC

更新日期：2007-09-15 00:00:00

abstract：:Isothermal titration calorimetry was used to characterize and quantify the partition of indomethacin and acemetacin between the bulk aqueous phase and the membrane of egg phosphatidylcholine vesicles. Significant electrostatic effects were observed due to binding of the charged drugs to the membrane, which implied the...

journal_title：Biophysical journal

authors： Matos C,Lima JL,Reis S,Lopes A,Bastos M

更新日期：2004-02-01 00:00:00

abstract：:Lipid mixing between vesicles functionalized with SNAREs and the cytosolic C2AB domain of synaptotagmin-1 recapitulates the basic Ca(2+) dependence of neuronal exocytosis. However, in the conventional ensemble lipid mixing assays it is not possible to discriminate whether Ca(2+) accelerates the docking or the fusion o...

journal_title：Biophysical journal

authors： Christensen SM,Mortensen MW,Stamou DG

更新日期：2011-02-16 00:00:00

abstract：:The bacterial flagellar motor (BFM) is a molecular machine that rotates the helical filaments and propels the bacteria swimming toward favorable conditions. In our previous works, we built a stochastic conformational spread model to explain the dynamic and cooperative behavior of BFM switching. Here, we extended this ...

journal_title：Biophysical journal

authors： Ma Q,Sowa Y,Baker MA,Bai F

更新日期：2016-03-29 00:00:00

abstract：:The theoretical basis of an optical microscope technique to image dynamically scattered light fluctuation decay rates (dynamic light scattering microscopy) is developed. It is shown that relative motions between scattering centers even smaller than the optical resolution of the microscope are sufficient to produce sig...

journal_title：Biophysical journal

authors： Dzakpasu R,Axelrod D

更新日期：2004-08-01 00:00:00

abstract：:Hepatitis C is a major worldwide disease and health hazard, affecting ∼3% of the world population. The p7 protein of hepatitis C virus (HCV) is an intracellular ion channel and pH regulator that is involved in the viral replication cycle. It is targeted by various classical ion channel blockers. Here, we generated p7 ...

journal_title：Biophysical journal

authors： Breitinger U,Farag NS,Ali NKM,Breitinger HA

更新日期：2016-06-07 00:00:00