Calcium-actin waves and oscillations of cellular membranes.

abstract：:Detection of specific nucleic acid sequences is invaluable in biological studies such as genetic disease diagnostics and genome profiling. Here, we developed a highly sensitive and specific detection method that combines an advanced oligonucleotide ligation assay with multicolor single-molecule fluorescence. We demons...

journal_title：Biophysical journal

authors： Severins I,Szczepaniak M,Joo C

更新日期：2018-09-18 00:00:00

abstract：:The mechanism by which the cytoskeletal protein actin affects the conductance of amiloride-sensitive epithelial sodium channels (ENaC) was studied in planar lipid bilayers. In the presence of monomeric actin, we found a decrease in the single-channel conductance of alpha-ENaC that did not occur when the internal [Ca2+...

journal_title：Biophysical journal

authors： Berdiev BK,Latorre R,Benos DJ,Ismailov II

更新日期：2001-05-01 00:00:00

abstract：:Mycolactone, a cytotoxic and immunosuppressive macrolide produced by Mycobacterium ulcerans, is the central virulent factor in the skin disease Buruli ulcer. This multifunctional cytotoxin affects fundamental cellular processes such as cell adhesion, immune response, and cell death by targeting various cellular struct...

journal_title：Biophysical journal

authors： Aydin F,Sun R,Swanson JMJ

更新日期：2019-07-09 00:00:00

abstract：:Cationic amino acid transporters are highly selective for L-enantiomers such as L-arginine (L-Arg). Because of this stereoselectivity, little is known about the interaction of these transporters with D-isomers. To study whether these compounds can provide information on the molecular mechanism of transport, inward cur...

journal_title：Biophysical journal

authors： Zhou J,Peluffo RD

更新日期：2010-11-17 00:00:00

abstract：:A major challenge in the study of biomolecular assemblies is to identify reaction coordinates that precisely monitor conformational rearrangements. This is central to the interpretation of single-molecule fluorescence resonance energy transfer measurements, where the observed dynamics depends on the labeling strategy....

journal_title：Biophysical journal

authors： Levi M,Nguyen K,Dukaye L,Whitford PC

更新日期：2017-12-19 00:00:00

abstract：:Biological membranes composed of lipids and proteins are in contact with electrolytes like aqueous NaCl solutions. Based on molecular dynamics studies it is widely believed that Na(+) ions specifically bind to 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes, whereas Cl(-) ions stay in solution. Here,...

journal_title：Biophysical journal

authors： Knecht V,Klasczyk B

更新日期：2013-02-19 00:00:00

abstract：:The shapes of fluorescence induction curves in spinach chloroplasts, measured using double-flash pump-probe techniques, are shown to depend on the duration of the actinic flashes. For flash durations tau(0)

journal_title：Biophysical journal

authors： Valkunas L,Geacintov NE,France L,Breton J

更新日期：1991-02-01 00:00:00

abstract：:Molecular dynamics simulations of wild-type bacteriorhodopsin (bR) and of its D85N, D85T, D212N, and Y57F mutants have been carried out to investigate possible differences in the photoproducts of these proteins. For each mutant, a series of 50 molecular dynamics simulations of the photoisomerization and subsequent rel...

journal_title：Biophysical journal

authors： Humphrey W,Bamberg E,Schulten K

更新日期：1997-03-01 00:00:00

abstract：:Cellular sensory systems often respond not to the absolute levels of inputs but to the fold-changes in inputs. Such a property is called fold-change detection (FCD) and is important for accurately sensing dynamic changes in environmental signals in the presence of fluctuations in their absolute levels. Previous studie...

journal_title：Biophysical journal

authors： Hironaka K,Morishita Y

更新日期：2014-01-07 00:00:00

abstract：:A new method of fluorescence microscopy for cell imaging has been developed that takes advantage of the spatial variations of fluorescence lifetimes in single cells as a source of image contrast, and thus it is named "fluorescence lifetime imaging microscopy (flimscopy)". Since time-resolved fluorescence measurements ...

journal_title：Biophysical journal

authors： Oida T,Sako Y,Kusumi A

更新日期：1993-03-01 00:00:00

abstract：:The interaction of α-helical peptides with lipid bilayers is central to our understanding of the physicochemical principles of biological membrane organization and stability. Mutations that alter the position or orientation of an α-helix within a membrane, or that change the probability that the α-helix will insert in...

journal_title：Biophysical journal

authors： Hall BA,Chetwynd AP,Sansom MS

更新日期：2011-04-20 00:00:00

abstract：:Structured illumination microscopy is a method that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit by using spatially structured illumination light. Here we describe how this method can be applied in three dimensions to double the axial as well as the lateral resol...

journal_title：Biophysical journal

authors： Gustafsson MG,Shao L,Carlton PM,Wang CJ,Golubovskaya IN,Cande WZ,Agard DA,Sedat JW

更新日期：2008-06-01 00:00:00

abstract：:Understanding the intermolecular interaction potential, V(r), of proteins under the influence of temperature, pressure, and salt concentration is essential for understanding protein aggregation, crystallization, and protein phase behavior in general. Here, we report small-angle x-ray scattering studies on dense lysozy...

journal_title：Biophysical journal

authors： Möller J,Schroer MA,Erlkamp M,Grobelny S,Paulus M,Tiemeyer S,Wirkert FJ,Tolan M,Winter R

更新日期：2012-06-06 00:00:00

abstract：:Cells in a cloned population of coliform bacteria exhibit a wide range of swimming behaviors--a form of non-genetic individuality. We used computer models to examine the proposition that these variations are due to differences in the number of chemotaxis signaling molecules from one cell to the next. Simulations were ...

journal_title：Biophysical journal

authors： Levin MD,Morton-Firth CJ,Abouhamad WN,Bourret RB,Bray D

更新日期：1998-01-01 00:00:00

abstract：:The following arguments are presented for the observation that curves relating free Ca2+ and force development of thin filament regulated myofilaments of skinned muscle fibers have Hill coefficient (n) greater than 4, which is the number of Ca2+ binding sites on troponin: Activation of the myofilaments is a process re...

journal_title：Biophysical journal

authors： Shiner JS,Solaro RJ

更新日期：1984-10-01 00:00:00

abstract：:Human apolipoprotein E (apoE) is a 299-amino-acid protein with a molecular weight of 34 kDa. The difference between the apoE3 and apoE4 isoforms is a single residue substitution involving a Cys-Arg replacement at residue 112. ApoE4 is positively associated with atherosclerosis and late-onset and sporadic Alzheimer's d...

journal_title：Biophysical journal

authors： Chou CY,Lin YL,Huang YC,Sheu SY,Lin TH,Tsay HJ,Chang GG,Shiao MS

更新日期：2005-01-01 00:00:00

abstract：:Low frequency audible vibrations are produced by human skeletal muscles undergoing sustained contraction. The effect is easily demonstrable with an electronic stethoscope which amplifies sound below 50 Hz. Autocorrelation analysis of the signal shows that it is periodic with a frequency 25 +/- 2.5 Hz. The quality of t...

journal_title：Biophysical journal

authors： Oster G,Jaffe JS

更新日期：1980-04-01 00:00:00

abstract：:Ca(2+) ion effect on protoplasmic streaming in an internodal cell of Nitella has been investigated for various temperatures. We have found that the protoplasmic streaming at low temperature is remarkably affected by the Ca(2+) ions in the internodal cell but larger concentrations of the Ca(2+) ions are needed to suppr...

journal_title：Biophysical journal

authors： Takamatsu A,Aoki T,Tsuchiya Y

更新日期：1993-01-01 00:00:00

abstract：:The structure and dynamics of soluble misfolded aggregates are poorly understood, despite their importance in protein science and disease. Water-soluble self-associated species that do not become insoluble over time are invaluable tools for high-resolution conformational studies aimed at dissecting the determinants of...

journal_title：Biophysical journal

authors： Rajagopalan S,Kurt N,Cavagnero S

更新日期：2011-02-02 00:00:00

abstract：:BBL is an independent folding domain of a large multienzyme complex, 2-oxoglutarate dehydrogenase. The folding mechanism of BBL is under debate between the views of noncooperative downhill-type and classical two-state. Extensive replica exchange molecular dynamics simulations of BBL in explicit solvent have shown some...

journal_title：Biophysical journal

authors： Fan J,Duan M,Li DW,Wu H,Yang H,Han L,Huo S

更新日期：2011-05-18 00:00:00

abstract：:The development of strategies to measure plasma membrane osmotic water permeability (Pf) in epithelial cells has been motivated by the identification of a family of molecular water channels. A general approach utilizing interferometry to measure cell shape and volume was developed and applied to measure Pf in cell lay...

journal_title：Biophysical journal

authors： Farinas J,Verkman AS

更新日期：1996-12-01 00:00:00

abstract：:Sulfolobus solfataricus carboxypeptidase (CPSso) is a thermostable zinc-metalloenzyme with a M(r) of 43,000. Taking into account the experimentally determined zinc content of one ion per subunit, we developed two alternative 3D models, starting from the available structures of Thermoactinomyces vulgaris carboxypeptida...

journal_title：Biophysical journal

authors： Occhipinti E,Martelli PL,Spinozzi F,Corsi F,Formantici C,Molteni L,Amenitsch H,Mariani P,Tortora P,Casadio R

更新日期：2003-08-01 00:00:00

abstract：:The mechanical properties of living cells are a label-free biophysical marker of cell viability and health; however, their use has been greatly limited by low measurement throughput. Although examining individual cells at high rates is now commonplace with fluorescence activated cell sorters, development of comparable...

journal_title：Biophysical journal

authors： Sawetzki T,Eggleton CD,Desai SA,Marr DW

更新日期：2013-11-19 00:00:00

abstract：:Morphogenesis involves the dynamic interplay of biochemical, mechanical, and electrical processes. Here, we ask to what extent can the course of morphogenesis be modulated and controlled by an external electric field? We show that at a critical amplitude, an external electric field can halt morphogenesis in Hydra rege...

journal_title：Biophysical journal

authors： Braun E,Ori H

更新日期：2019-10-15 00:00:00

abstract：:The excess oxygen concentration in the photosynthetic membranes of functioning oxygenic photosynthetic cells was estimated using classical diffusion theory combined with experimental data on oxygen production rates of cyanobacterial cells. The excess oxygen concentration within the plesiomorphic cyanobacterium Gloeoba...

journal_title：Biophysical journal

authors： Kihara S,Hartzler DA,Savikhin S

更新日期：2014-05-06 00:00:00

abstract：:Diffraction rings corresponding to the first, second, and third order were obtained by laser light illumination from a suspension of rabbit glycerinated psoas myofibrils (diameter, 1-2 microns; average length of the straight region, 44 microns; average sarcomere length, 2.2-2.6 microns) of which the optical thickness ...

journal_title：Biophysical journal

authors： Ishiwata S,Okamura N

更新日期：1989-12-01 00:00:00

abstract：:Several strategies directed toward increasing the participation of minority students in physics and biophysics are presented. Since the number of minority students entering college with an interest in science and mathematics must be increased if we expect to see more students graduating in science, several programs ai...

journal_title：Biophysical journal

authors： Tanaka JC,Gladney LD

更新日期：1993-07-01 00:00:00

abstract：:A variety of experiments suggest that membrane proteins are important targets of anesthetic molecules, and that ion channels interact differently with anesthetics in their open and closed conformations. The availability of an open and a closed structural model for the KirBac1.1 potassium channel has made it possible t...

journal_title：Biophysical journal

authors： Vemparala S,Domene C,Klein ML

更新日期：2008-06-01 00:00:00

abstract：:Ras is a membrane-anchored signaling protein that serves as a hub for many signaling pathways and also plays a prominent role in cancer. The intrinsic behavior of Ras on the membrane has captivated the biophysics community in recent years, especially the possibility that it may form dimers. In this article, we describ...

journal_title：Biophysical journal

authors： Chung JK,Lee YK,Denson JP,Gillette WK,Alvarez S,Stephen AG,Groves JT

更新日期：2018-01-09 00:00:00

abstract：:Na(+) conductance through cloned K(+) channels has previously allowed characterization of inactivation and K(+) binding within the pore, and here we have used Na(+) permeation to study recovery from C-type inactivation in human Kv1.5 channels. Replacing K(+) in the solutions with Na(+) allows complete Kv1.5 inactivati...

journal_title：Biophysical journal

authors： Wang Z,Hesketh JC,Fedida D

更新日期：2000-11-01 00:00:00