Solutions for transients in arbitrarily branching cables: III. Voltage clamp problems.

abstract：:Two independent methods, induced osmosis and solvent drag, were used to determine the reflection coefficients for NaCl (sigma NaCl) in brush border and basolateral membrane vesicles isolated from rabbit proximal tubule. In the induced osmosis method, vesicles loaded with sucrose were subjected to varying inward NaCl g...

journal_title：Biophysical journal

authors： Pearce D,Verkman AS

更新日期：1989-06-01 00:00:00

abstract：:In some fungi, a premeiotic process known as repeat-induced point mutation (RIP) can accurately identify and mutate nearly all gene-sized DNA repeats present in the haploid germline nuclei. Studies in Neurospora crassa have suggested that RIP detects sequence homology directly between intact DNA double helices, withou...

journal_title：Biophysical journal

authors： Mazur AK,Gladyshev E

更新日期：2018-08-21 00:00:00

abstract：:The steady-state stimulus-response curve of the Limulus ventral photoreceptor comprises a linear portion followed by a less-than-unity power law dependence, which is maintained over at least 4 decades of intensity. This progressive desensitization corresponds to light adaptation. For flash stimulation of dark-adapted ...

journal_title：Biophysical journal

authors： Grzywacz NM,Hillman P

更新日期：1988-03-01 00:00:00

abstract：:One of the disadvantages of the sucrose-gap method for measuring membrane potentials with extracellular electrodes is a membrane hyperpolarization of the order of 30 to 60 mv, as compared with the resting potential obtained with intracellular microelectrodes in the absence of a sucrose-gap. In the present study the co...

journal_title：Biophysical journal

authors： Blaustein MP,Goldman DE

更新日期：1966-07-01 00:00:00

abstract：:Many toxins and antimicrobial peptides permeabilize membrane vesicles by forming multimeric pores. Determination of the size of such pores is an important first step for understanding their structure and the mechanism of their self-assembly. We report a simple method for sizing pores in vesicles based on the different...

journal_title：Biophysical journal

authors： Ladokhin AS,Selsted ME,White SH

更新日期：1997-04-01 00:00:00

abstract：:In addition to the annular or boundary lipids that surround the transmembrane surface of the potassium channel KcsA from Streptomyces lividans, x-ray crystallographic studies have detected one anionic lipid molecule bound at each protein-protein interface in the homotetrameric structure, at sites referred to as nonann...

journal_title：Biophysical journal

authors： Marius P,Zagnoni M,Sandison ME,East JM,Morgan H,Lee AG

更新日期：2008-03-01 00:00:00

abstract：:The endoplasmic reticulum (ER) is the major compartment for the processing and quality control of newly synthesized proteins. Green fluorescent protein (GFP) was used as a noninvasive probe to determine the viscous properties of the aqueous lumen of the ER. GFP was targeted to the ER lumen of CHO cells by transient tr...

journal_title：Biophysical journal

authors： Dayel MJ,Hom EF,Verkman AS

更新日期：1999-05-01 00:00:00

abstract：:Some new approaches to the kinetic study of the reduction of cytochrome aa3 by cytochrome c are presented. The primary innovations are the use of a spectrometer which can acquire multiwavelength data as fast as every 10 microseconds, and the application of a variety of analytical methods which can utilize simultaneous...

journal_title：Biophysical journal

authors： Hendler RW,Bose SK,Shrager RI

更新日期：1993-09-01 00:00:00

abstract：:In most environments, such as natural aquatic systems, bacteria are found predominantly in self-organized sessile communities known as biofilms. In the presence of a significant flow, mature multispecies biofilms often develop into long filamentous structures called streamers, which can greatly influence ecosystem pro...

journal_title：Biophysical journal

authors： Rusconi R,Lecuyer S,Autrusson N,Guglielmini L,Stone HA

更新日期：2011-03-16 00:00:00

abstract：:Human transthyretin (TTR) is an amyloidogenic protein. The pathway of TTR amyloid formation has been proposed based on lines of evidence: TTR tetramer first dissociates into native monomers, which is shown to be a rate-limiting step in the formation of fibrils. Subsequently, the monomeric species partially unfold to f...

journal_title：Biophysical journal

authors： Yang M,Lei M,Bruschweiler R,Huo S

更新日期：2005-07-01 00:00:00

abstract：:Cell migration is based on an actin treadmill, which in turn depends on recycling of G-actin across the cell, from the rear where F-actin disassembles, to the front, where F-actin polymerizes. To analyze the rates of the actin transport, we used the Virtual Cell software to solve the diffusion-drift-reaction equations...

journal_title：Biophysical journal

authors： Novak IL,Slepchenko BM,Mogilner A

更新日期：2008-08-01 00:00:00

abstract：:EXACT FORMULAS ARE DERIVED FOR THE ENERGY CHANGE OF A DIPOLE SYSTEM WITH TWO ENERGY STATES (OR BANDS) IN A CHANGING FIELD IN TWO CASES: (a) no dipole flip-flop and (b) dipole flip-flop caused by stimulation. Based on these formulas, the positive and negative heats are calculated. The results are in good agreement with...

journal_title：Biophysical journal

authors： Wei LY

更新日期：1972-09-01 00:00:00

abstract：:The effect of a nonuniform solute concentration on the osmotic transport of water through the boundaries of a simple model cell is investigated. A system of two ordinary differential equations is derived for the motion of a single cell in the limit of a fast solute diffusion, and an analytic solution is obtained for o...

journal_title：Biophysical journal

authors： Jaeger M,Carin M,Medale M,Tryggvason G

更新日期：1999-09-01 00:00:00

abstract：:Previously (Hines, M., and J.J. Blum 1983, Biophys. J., 41:67-79), a method was developed that allowed one to compute curvature and twist for a three-dimensional sliding filament model. In that formalism it was difficult to specify the shear and bending moments arising from moment-bearing interfilament links such as f...

journal_title：Biophysical journal

authors： Hines M,Blum JJ

更新日期：1984-11-01 00:00:00

abstract：:Uracil DNA Glycosylase (UNG2) is the primary enzyme in humans that prevents the stable incorporation of deoxyuridine monophosphate into DNA in the form of U/A basepairs. During S-phase, UNG2 remains associated with the replication fork through its interactions with two proteins, Proliferating Cell Nuclear Antigen (PCN...

journal_title：Biophysical journal

authors： Weiser BP,Stivers JT,Cole PA

更新日期：2017-07-25 00:00:00

abstract：:Water plays an important role in enzyme structure and function in aqueous media. That role becomes even more important when one focuses on enzymes in low water media. Here we present results from molecular dynamics simulations of surfactant-solubilized subtilisin BPN' in three organic solvents (octane, tetrahydrofuran...

journal_title：Biophysical journal

authors： Yang L,Dordick JS,Garde S

更新日期：2004-08-01 00:00:00

abstract：:The Mie theory of scattering is used to provide new information on how changes in particle volume, with no change in dry weight, should influence light scattering for various scattering angles and particle sizes. Many biological cells (e.g., algal cells, erythrocytes) and large subcellular structures (e.g., chloroplas...

journal_title：Biophysical journal

authors： Latimer P,Pyle BE

更新日期：1972-07-01 00:00:00

abstract：:The study of mechanotransduction relies on tools that are capable of applying mechanical forces to elicit and assess cellular responses. Here we report a new (to our knowledge) technique, called two-bubble acoustic tweezing cytometry (TB-ATC), for generating spatiotemporally controlled subcellular mechanical forces on...

journal_title：Biophysical journal

authors： Chen D,Sun Y,Gudur MS,Hsiao YS,Wu Z,Fu J,Deng CX

更新日期：2015-01-06 00:00:00

abstract：:Hemoglobin functions as a tetrameric oxygen transport protein, with each subunit containing a heme cofactor. Its denaturation, either in vivo or in vitro, involves autoxidation to methemoglobin, followed by cofactor loss and globin unfolding. We have proposed a global disassembly scheme for human methemoglobin, linkin...

journal_title：Biophysical journal

authors： Samuel PP,White MA,Ou WC,Case DA,Phillips GN Jr,Olson JS

更新日期：2020-03-24 00:00:00

abstract：:Crystals of ion-free gramicidin A (P212121: a = 24.61, b = 32.28, c = 32.52) have been investigated using neutron diffraction. A difference analysis of crystals soaked in ethanol/H2O as opposed to ethanol-d6/D2O has led to single isomorphous replacement Fourier projections of the structure at 5-A resolution. The grami...

journal_title：Biophysical journal

authors： Koeppe RE 2nd,Schoenborn BP

更新日期：1984-03-01 00:00:00

abstract：:DNA bending is assumed to play a crucial role during recognition of the cAMP-responsive element (CRE) by transcription factors. However, diverging results have been obtained for the bending direction of the unbound double helix. The refined NMR structures present a bend directed toward the minor groove, while biochemi...

journal_title：Biophysical journal

authors： Derreumaux S,Fermandjian S

更新日期：2000-08-01 00:00:00

abstract：:The crystal structure of the RNA octamer, 5'-GGCGUGCC-3' has been determined from x-ray diffraction data to 1.5 angstroms resolution. In the crystal, this oligonucleotide forms five self-complementary double-helices in the asymmetric unit. Tandem 5'GU/3'UG basepairs comprise an internal loop in the middle of each dupl...

journal_title：Biophysical journal

authors： Jang SB,Hung LW,Jeong MS,Holbrook EL,Chen X,Turner DH,Holbrook SR

更新日期：2006-06-15 00:00:00

abstract：:Intrinsically disordered proteins play important roles throughout biology, yet our understanding of the relationship between their sequences, structural properties, and functions remains incomplete. The dynamic nature of these proteins, however, makes them difficult to characterize structurally. Many disordered protei...

journal_title：Biophysical journal

authors： Nygaard M,Kragelund BB,Papaleo E,Lindorff-Larsen K

更新日期：2017-08-08 00:00:00

abstract：:Competitive hybridization, at the surface and in the bulk, lowers the sensitivity of DNA chips. Competitive surface hybridization occurs when different targets can hybridize with the same probe. Competitive bulk hybridization takes place when the targets can hybridize with free complementary chains in the solution. Th...

journal_title：Biophysical journal

authors： Halperin A,Buhot A,Zhulina EB

更新日期：2004-02-01 00:00:00

abstract：:An equation is derived, which connects two functions P(r) and g(x), the first being related to the scattering intensity by a simple transformation, the second to the elctron density distribution of a spherically symmetric structure. This relation seems to be a suitable starting point for an analysis of shell structure...

journal_title：Biophysical journal

authors： Weick D

更新日期：1974-03-01 00:00:00

abstract：:ATP synthase (F-ATPase) operates as an electrochemical-to-mechanical-to-chemical energy transducer with an astounding 360 degrees rotary motion of subunits epsilongammac(10-14) (rotor) against delta(alphabeta)(3)ab(2) (stator). The enzyme's torque as a function of the angular reaction coordinate in relation to ATP-syn...

journal_title：Biophysical journal

authors： Cherepanov DA,Junge W

更新日期：2001-09-01 00:00:00

abstract：:Effects of adducts of [PtCl(NH3)3]Cl or chlorodiethylenetriamineplatinum(II) on DNA stability were studied with emphasis on thermodynamic origins of that stability. Oligodeoxyribonucleotide duplexes (15-bp) containing the single, site-specific monofunctional adduct at G-residues of the central sequences TGT/ACA or 5'-...

journal_title：Biophysical journal

authors： Bursova V,Kasparkova J,Hofr C,Brabec V

更新日期：2005-02-01 00:00:00

abstract：:The development of scanning probe techniques has made it possible to examine protein-protein interactions at the level of individual molecular pairs. A calibrated optical tweezers, along with immunoglobulin G (IgG)-coated polystyrene microspheres, has been used to detect individual surface-linked Staphylococcus protei...

journal_title：Biophysical journal

authors： Stout AL

更新日期：2001-06-01 00:00:00

abstract：:A detailed analysis is made of the model system of two parallel cylindrical polyelectrolytes which contain ionizable groups on their surfaces and are immersed in an ionic bathing medium. The interaction between the cylinders is examined by considering the interplay between repulsive electrostatic forces and attractive...

journal_title：Biophysical journal

authors： Brenner SL,McQuarrie DA

更新日期：1973-04-01 00:00:00

abstract：:More than 30 years ago, Nozaki and Tanford reported that the pK values for several amino acids and simple substances in 6 M guanidinium chloride differed little from the corresponding values in low salt (Nozaki, Y., and C. Tanford. 1967. J. Am. Chem. Soc. 89:736-742). This puzzling and counter-intuitive result hinders...

journal_title：Biophysical journal

authors： Garcia-Mira MM,Sanchez-Ruiz JM

更新日期：2001-12-01 00:00:00