Abstract：
:The effect of a nonuniform solute concentration on the osmotic transport of water through the boundaries of a simple model cell is investigated. A system of two ordinary differential equations is derived for the motion of a single cell in the limit of a fast solute diffusion, and an analytic solution is obtained for one special case. A two-dimensional finite element model has been developed to simulate the more general case (finite diffusion rates, solute gradient induced by a solidification front). It is shown that the cell moves to regions of lower solute concentration due to the uneven flux of water through the cell boundaries. This mechanism has apparently not been discussed previously. The magnitude of this effect is small for red blood cells, the case in which all of the relevant parameters are known. We show, however, that it increases with cell size and membrane permeability, so this effect could be important for larger cells. The finite element model presented should also have other applications in the study of the response of cells to an osmotic stress and for the interaction of cells and solidification fronts. Such investigations are of major relevance for the optimization of cryopreservation processes.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Jaeger M,Carin M,Medale M,Tryggvason Gdoi
10.1016/S0006-3495(99)76977-8subject
Has Abstractpub_date
1999-09-01 00:00:00pages
1257-67issue
3eissn
0006-3495issn
1542-0086pii
S0006-3495(99)76977-8journal_volume
77pub_type
杂志文章abstract：:Phosphatidylinositol phospholipase Cγ (PLCγ) is an intracellular membrane-associated second-messenger signaling protein activated by tyrosine kinases such as fibroblast growth factor receptor 1. PLCγ contains the regulatory γ-specific array (γSA) comprising a tandem Src homology 2 (SH2) pair, an SH3 domain, and a spli...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2018.05.031
更新日期：2018-07-03 00:00:00
abstract：:The group-additive decomposition of the unfolding free energy of a protein in an osmolyte solution relative to that in water poses a fundamental paradox: whereas the decomposition describes the experimental results rather well, theory suggests that a group-additive decomposition of free energies is, in general, not va...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2013.08.011
更新日期：2013-09-17 00:00:00
abstract：:Morphine spin labeled on the phenolic hydroxy group has been prepared using commercially available reagents and characterized by thin layer chromatography, mass spectroscopy, and electron spin resonance spectroscopy. It has been shown that morphine modified in this way retains some opiate activity, does not pass throu...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(75)85889-9
更新日期：1975-11-01 00:00:00
abstract：:Hemolymph and protein-free hemolymph obtained from Loligo pealei were analyzed for cation concentration by the method of atomic absorption spectroscopy, for the following ions: Na+, K+, Ca++, and Mg++. No significant differences were seen in ion concentration between hemolymph and protein-free hemolymph. Of particular...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(77)85609-9
更新日期：1977-05-01 00:00:00
abstract：:Myosin II is a central mechanoenzyme in a wide range of cellular morphogenic processes. Its cellular localization is dependent not only on signal transduction pathways, but also on mechanical stress. We suggest that this stress-dependent distribution is the result of both the force-dependent binding to actin filaments...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2011.12.020
更新日期：2012-01-18 00:00:00
abstract：:We examined the effects of brief current pulses on the pacemaker oscillations of the Purkinje fiber using the model of McAllister , Noble, and Tsien (1975. J. Physiol. [Lond.]. 251:1-57). This model was used to construct phase-response curves for brief electric stimuli to find "black holes," where rhythmic activity of...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(84)84228-9
更新日期：1984-04-01 00:00:00
abstract：:It is often assumed that pH(i) is spatially uniform within cells. A double-barreled microperfusion system was used to apply solutions of weak acid (acetic acid, CO(2)) or base (ammonia) to localized regions of an isolated ventricular myocyte (guinea pig). A stable, longitudinal pH(i) gradient (up to 1 pH(i) unit) was ...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.104.051391
更新日期：2005-04-01 00:00:00
abstract：:The vessel wall experiences progressive stiffening with age and the development of cardiovascular disease, which alters the micromechanical environment experienced by resident vascular smooth muscle cells (VSMCs). In vitro studies have shown that VSMCs are sensitive to substrate stiffness, but the exact molecular mech...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2011.06.051
更新日期：2011-08-03 00:00:00
abstract：:The physical characteristics which govern the water relations of the giant-celled sporangiophore of Phycomyces blakesleeanus were measured with the pressure probe technique and with nanoliter osmometry. These properties are important because they govern water uptake associated with cell growth and because they may in...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(87)83363-5
更新日期：1987-03-01 00:00:00
abstract：:Transient receptor potential (TRP) channels are members of a large family of ion channels located in membranes rich in cholesterol, some of whose functions are affected by the cholesterol content of the membrane. Here, cholesterol binding to TRPs is studied using a docking procedure that allows the transmembrane surfa...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2019.10.011
更新日期：2019-11-19 00:00:00
abstract：:Fluorescence lifetime imaging microscopy is used to demonstrate that different loads applied to a muscle fiber change the microenvironment of the nucleotide binding pocket of myosin. Permeabilized skeletal muscle fibers in rigor were labeled with a fluorescent ATP analog, 3'-DEAC-propylenediamine (pda)-ATP (3'-O-{N-[3...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2010.07.052
更新日期：2010-10-06 00:00:00
abstract：:Thermodynamically rigorous free energy methods in principle allow the exact computation of binding free energies in biological systems. Here, we use thermodynamic integration together with molecular dynamics simulations of a DNA-protein complex to compute relative binding free energies of a series of mutants of a prot...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2011.07.003
更新日期：2011-09-07 00:00:00
abstract：:A 10-ns molecular dynamics simulation of mouse acetylcholinesterase was analyzed, with special attention paid to the fluctuation in the width of the gorge and opening events of the back door. The trajectory was first verified to ensure its stability. We defined the gorge proper radius as the measure for the extent of ...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(01)75736-0
更新日期：2001-08-01 00:00:00
abstract：:Specific proteins and lipids sequester to regions of cell membranes called rafts. Due to their high content of sphingomyelin (SM) and cholesterol, raft bilayers are thicker than nonraft bilayers and, at least at 4 degrees C, are resistant to Triton X-100 extraction. It has been postulated that rafts concentrate protei...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(03)74595-0
更新日期：2003-09-01 00:00:00
abstract：:The conformational flexibility of the DNA double helix is of great interest because of its potential role in protein recognition, packaging into chromosomes, formation of photodefects, and interaction with drugs. Theory finds that DNA is very flexible; however, there is a scarcity of experimental results that examine ...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(96)79755-2
更新日期：1996-04-01 00:00:00
abstract：:Constant electric fields and constant ion currents are often considered in theories of ion transport. Therefore, it is important to understand the validity of these helpful concepts. The constant field assumption requires that the charge density of permeant ions and flexible polar groups is virtually voltage independe...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(99)77242-5
更新日期：1999-02-01 00:00:00
abstract：:We measured the influence of alternative versions of the Drosophila melanogaster myosin heavy chain relay domain on muscle mechanical properties. We exchanged relay domain regions (encoded by alternative versions of exon 9) between an embryonic (EMB) isoform and the indirect flight muscle isoform (IFI) of myosin. Prev...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.108.136192
更新日期：2008-12-01 00:00:00
abstract：:Dimerization of the transmembrane (TM) adaptor protein DAP12 plays a key role in mediating activation signals through TM-TM association with cell-surface receptors. Herein, we apply the TOXCAT assay and molecular dynamics simulation to analyze dynamics and dimerization of the TM helix of DAP12 in the membrane bilayer....
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2013.01.054
更新日期：2013-04-02 00:00:00
abstract：:Recent studies suggest that phosphoinositide kinases may participate in intracellular trafficking or exocytotic events. Because both of these events ultimately require fusion of biological membranes, the susceptibility of membranes containing polyphosphoinositides (PPIs) to divalent cation-induced fusion was investiga...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(96)79513-9
更新日期：1996-12-01 00:00:00
abstract：:Changes in the ultraviolet light scattering from a suspension of purple membrane fragments were detected during the photocycle of bacteriorhodopsin with a cross-correlation method. The scattered light intensity from a suspension of membrane fragments containing the protein bacteriorhodopsin was measured on a logarithm...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(90)82415-2
更新日期：1990-09-01 00:00:00
abstract：:Bioluminescence rhythms from cellular reporters have become the most common method used to quantify oscillations in circadian gene expression. These experimental systems can reveal phase and amplitude change resulting from circadian disturbances, and can be used in conjunction with mathematical models to lend further ...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2014.10.026
更新日期：2014-12-02 00:00:00
abstract：:Stearoylsphingomyelin (SSM) bilayers containing 0, 22, and 50 mol % cholesterol (Chol) and a pentadecanoyl-stearoylphosphatidylcholine (15SPC) bilayer containing 22 mol % Chol were molecular dynamics simulated at two temperatures (37 degrees C and 60 degrees C). 15SPC is the best PC equivalent of SSM. The Chol effect ...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.106.080887
更新日期：2006-11-15 00:00:00
abstract：:Some plant leaves display complex, orientation-dependent, proton nuclear magnetic resonance (1H NMR) spectra. The spectral patterns vary as the angle between the leaf surface and the applied magnetic field is varied. They also vary with temperature and with the quantity of absorbed manganous ions, but they are indepen...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(85)83826-1
更新日期：1985-11-01 00:00:00
abstract：:The Qx-Qy splitting observed in the fluorescence excitation spectra of Mg-mesoporphyrin-IX substituted horseradish peroxidase (MgMP-HRP) and of its complex with naphthohydroxamic acid (NHA) was studied by spectral hole burning techniques. The width of a hole directly burnt in the Qy band and that of a satellite hole i...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(01)76032-8
更新日期：2001-01-01 00:00:00
abstract：:A major challenge in synthetic gene delivery is to quantitatively predict the optimal design of polymer-based gene carriers (polyplexes). Here, we report a consistent, integrated, and fundamentally grounded computational methodology to address this challenge. This is achieved by accurately representing the spatio-temp...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1529/biophysj.106.095521
更新日期：2007-02-01 00:00:00
abstract：:We have examined the mechanisms underlying the voltage sensitivity of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors in voltage-clamped outside-out patches and whole cells taken from the nucleus magnocellularis of the chick. Responses to either glutamate or kainate had outwardly rectifying current-volt...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(95)80057-3
更新日期：1995-11-01 00:00:00
abstract：:Glucose-embedded bacteriorhodopsin shows M-intermediates with different Amide I infrared bands when samples are illuminated at 240 or 260 K, in contrast with fully hydrated samples where a single M-intermediate is formed at all temperatures. In hydrated, but not in glucose-embedded specimens, the N intermediate is for...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(94)80585-5
更新日期：1994-09-01 00:00:00
abstract：:Dynamic properties of class A beta-lactamase TEM-1 are investigated from molecular dynamics (MD) simulations. Comparison of MD-derived order parameters with those obtained from model-free analysis of nuclear magnetic resonance (NMR) relaxation data shows high agreement for N-H moieties within alpha- and beta-secondary...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/j.bpj.2009.08.061
更新日期：2010-02-17 00:00:00
abstract：:A free radical probe was introduced into single barnacle muscle cells, and its freedom of motion inferred from the spin resonance spectra. The probe reported an average local viscosity of 5-10 cp compared with 1 cp for pure water. From a comparison of the temperature dependence of the probe's tumbling rate in model aq...
journal_title：Biophysical journal
pub_type： 杂志文章
doi：10.1016/S0006-3495(74)85918-7
更新日期：1974-04-01 00:00:00
abstract：:The calyx of Held is a giant nerve terminal that forms a glutamatergic synapse in the auditory pathway. Due to its large size, it offers a number of advantages for biophysical studies, including voltage-clamp of both pre- and postsynaptic compartments and the loading with indicator dyes and caged compounds. Three aspe...
journal_title：Biophysical journal
pub_type： 杂志文章,评审
doi：10.1016/j.bpj.2016.12.017
更新日期：2017-01-24 00:00:00
