Abstract:
:Some plant leaves display complex, orientation-dependent, proton nuclear magnetic resonance (1H NMR) spectra. The spectral patterns vary as the angle between the leaf surface and the applied magnetic field is varied. They also vary with temperature and with the quantity of absorbed manganous ions, but they are independent of magnetic field strength. In this paper, we propose a theory to explain the origin of the spectra and a model from which the patterns can be calculated. The theory shows how heterogeneous magnetic susceptibilities and local dipolar magnetic fields in chloroplasts can shift the water-proton resonance field. The model describes a simplified leaf structure in which the chloroplasts are nonrandomly aligned with respect to the leaf surface. Model calculations are tested by comparison with experimental spectra from hawthorn leaves (Crataegus sp.).
journal_name
Biophys Jjournal_title
Biophysical journalauthors
McCain DC,Markley JLdoi
10.1016/S0006-3495(85)83826-1subject
Has Abstractpub_date
1985-11-01 00:00:00pages
687-94issue
5eissn
0006-3495issn
1542-0086pii
S0006-3495(85)83826-1journal_volume
48pub_type
杂志文章abstract::Neurodegenerative disease can originate from the misfolding and aggregation of proteins, such as Amyloid-beta, SOD1, or Huntingtin. Fortunately, all cells possess protein quality control machinery that sequesters misfolded proteins, either refolding or degrading them, before they can self-associate into proteotoxic ol...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.105.066662
更新日期:2006-02-01 00:00:00
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journal_title:Biophysical journal
pub_type: 信件
doi:10.1016/j.bpj.2012.06.030
更新日期:2012-08-08 00:00:00
abstract::The microenvironment between the plasma membrane and the near-membrane sarcoplasmic reticulum (SR) may play an important role in Ca(2+) regulation in smooth muscle cells. We used a three-dimensional mathematical model of Ca(2+) diffusion and regulation and experimental measurements of SR Ca(2+) uptake and the distribu...
journal_title:Biophysical journal
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(86)83634-7
更新日期:1986-01-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(98)77588-5
更新日期:1998-08-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(83)84366-5
更新日期:1983-04-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.104.049031
更新日期:2004-12-01 00:00:00
abstract::The kinetics of aggregation of alpha-synuclein are usually studied by turbidity or Thio-T fluorescence. Here we follow the disappearance of monomers and the formation of early oligomers using fluorescence correlation spectroscopy. Alexa488-labeled A140C-synuclein was used as a fluorescent probe in trace amounts in the...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2009.12.4290
更新日期:2010-04-07 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.104.056747
更新日期:2005-07-01 00:00:00
abstract::Clathrin-coated membranes are precursors to coated vesicles in the receptor-mediated endocytic pathway. In this paper we present a physical model for the first steps of the transformation of a clathrin-coated membrane into a coated vesicle. The theory is based on in vitro cytoplasmic acidification experiments of Heuse...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(98)77993-7
更新日期:1998-06-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(96)79546-2
更新日期:1996-12-01 00:00:00
abstract::Characterizing the ionic distribution around chromatin is important for understanding the electrostatic forces governing chromatin structure and function. Here we develop an electrostatic model to handle multivalent ions and compute the ionic distribution around a mesoscale chromatin model as a function of conformatio...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2010.08.023
更新日期:2010-10-20 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.106.095521
更新日期:2007-02-01 00:00:00
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journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(99)77351-0
更新日期:1999-04-01 00:00:00
abstract::By combining the osmotic stress technique with small-angle x-ray scattering measurements, we followed the structural response of the casein micelle to an overall increase in concentration. When the aqueous phase that separates the micelles is extracted, they behave as polydisperse repelling spheres and their internal ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2010.10.019
更新日期:2010-12-01 00:00:00
abstract::Under various physiological and nonphysiological conditions, the intramembranous particles, as seen by freeze-fracture electron microscopy, may be in various degrees of aggregation. To compare various schemes for the measurement of the degree of aggregation, a computer program has been used to generate simulated aggre...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(81)84827-8
更新日期:1981-09-01 00:00:00
abstract::Pre-mRNA processing protein 40 (Prp40) is a nuclear protein that has a role in pre-mRNA splicing. Prp40 possesses two leucine-rich nuclear export signals, but little is known about the function of Prp40 in the export process. Another protein that has a role in protein export is centrin, a member of the EF-hand superfa...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2017.03.042
更新日期:2017-06-20 00:00:00
abstract::The determination of intracellular protein concentrations is a prerequisite for understanding protein interaction networks in systems biology. Today, protein quantification is based either on mass spectrometry, which requires large cell numbers and sophisticated measurement protocols, or on quantitative Western blotti...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2011.09.058
更新日期:2011-12-07 00:00:00
abstract::Laurdan fluorescence, novel spectral fitting, and dynamic light scattering were combined to determine lateral lipid organization in mixed lipid membranes of the oxidized lipid, 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC), and each of the three bilayer lipids, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2020.03.009
更新日期:2020-04-21 00:00:00
abstract::Autoregulatory feedback loops are one of the most common network motifs. A wide variety of stochastic models have been constructed to understand how the fluctuations in protein numbers in these loops are influenced by the kinetic parameters of the main biochemical steps. These models differ according to 1) which subce...
journal_title:Biophysical journal
pub_type: 杂志文章,评审
doi:10.1016/j.bpj.2020.02.016
更新日期:2020-04-07 00:00:00
abstract::The interaction between two stiff parallel DNA molecules is discussed using linear Debye-Hückel screening theory with and without inclusion of the dielectric discontinuity at the DNA surface, taking into account the helical symmetry of DNA. The pair potential furthermore includes the amount and distribution of counter...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(03)75092-9
更新日期:2003-06-01 00:00:00
abstract::A method for generating angular forces around sigma-bonded transition metal ions is generalized to treat pi-bonded configurations. The theoretical approach is based on an analysis of ligand-field and small-cluster Hamiltonians based on the moments of the electron state distribution. The functional forms that are obtai...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(01)75675-5
更新日期:2001-07-01 00:00:00
abstract::Activation of skeletal muscle ryanodine receptors (RyRs) by suramin and disulfonic stilbene derivatives (Diisothiocyanostilbene-2',2'-disulfonic acid (DIDS), 4,4'-dibenzamidostilbene-2,2'-disulfonic acid (DBDS),and 4,4'-dinitrostilbene-2,2'-disulfonic acid (DNDS)) was investigated using planar bilayers. One reversible...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(03)74976-5
更新日期:2003-03-01 00:00:00
abstract::Melittin has been reported to form toroidal pores under certain conditions, but the atomic-resolution structure of these pores is unknown. A 9-μs all-atom molecular-dynamics simulation starting from a closely packed transmembrane melittin tetramer in DMPC shows formation of a toroidal pore after 1 μs. The pore remains...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2015.04.006
更新日期:2015-05-19 00:00:00
abstract::The last decade established that the dynamic properties of the phosphoproteome are central to function and its modulation. The temporal dimension of phosphorylation effects remains nonetheless poorly understood, particularly for intrinsically disordered proteins. Osteopontin, selected for this study due to its key rol...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2017.03.005
更新日期:2017-04-25 00:00:00
abstract::The lipid matrix present in the uppermost layer of the skin, the stratum corneum, plays a crucial role in the skin barrier function. The lipids are organized into two lamellar phases. To gain more insight into the molecular organization of one of these lamellar phases, we performed neutron diffraction studies. In the ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2011.02.001
更新日期:2011-03-16 00:00:00
abstract::We study the folding thermodynamics and kinetics of the Pin1 WW domain, a three-stranded beta-sheet protein, by using all-atom (except nonpolar hydrogens) discontinuous molecular dynamics simulations at various temperatures with a Gō model. The protein exhibits a two-state folding kinetics near the folding transition ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1529/biophysj.106.102095
更新日期:2007-09-15 00:00:00
abstract::The outer membrane (OM) of Gram-negative bacteria is composed of lipopolysaccharide (LPS) in the outer leaflet and phospholipids in the inner leaflet. The outer membrane protein H (OprH) of Pseudomonas aeruginosa provides an increased stability to the OMs by directly interacting with LPS. Here we report the influence ...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2016.12.006
更新日期:2017-01-24 00:00:00
abstract::Films of O-phospho-L-serine-P-ethylester (PSE) were studied by infrared spectroscopy. PSE films were studied pure and as 1:1 mixture with LiOH, NaOH, KOH, and Ca(OH)2 as a function of the degree of hydration. The same investigations were performed if (L-glu)n was added to the system (ratio 1:1, PSE/glu residue). In th...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/S0006-3495(89)82861-9
更新日期:1989-04-01 00:00:00
abstract::Prion diseases are infectious fatal neurodegenerative diseases including Creutzfeldt-Jakob disease in humans and bovine spongiform encephalopathy in cattle. The misfolding and conversion of cellular PrP in such mammals into pathogenic PrP is believed to be the key procedure. Rabbits are among the few mammalian species...
journal_title:Biophysical journal
pub_type: 杂志文章
doi:10.1016/j.bpj.2011.08.018
更新日期:2011-09-21 00:00:00