Cytoplasmic solvent structure of single barnacle muscle cells studied by electron spin resonance.

Abstract:

:A free radical probe was introduced into single barnacle muscle cells, and its freedom of motion inferred from the spin resonance spectra. The probe reported an average local viscosity of 5-10 cp compared with 1 cp for pure water. From a comparison of the temperature dependence of the probe's tumbling rate in model aqueous systems and in the muscle we concluded that in the muscle the probe was undergoing fast exchange between sites of different mobility. Thus 10 cp must be taken as an upper limit for the viscosity of most cell water.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Sachs F,Latorre R

doi

10.1016/S0006-3495(74)85918-7

subject

Has Abstract

pub_date

1974-04-01 00:00:00

pages

316-26

issue

4

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(74)85918-7

journal_volume

14

pub_type

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