Abstract:
:The crystal structure of the RNA octamer, 5'-GGCGUGCC-3' has been determined from x-ray diffraction data to 1.5 angstroms resolution. In the crystal, this oligonucleotide forms five self-complementary double-helices in the asymmetric unit. Tandem 5'GU/3'UG basepairs comprise an internal loop in the middle of each duplex. The NMR structure of this octameric RNA sequence is also known, allowing comparison of the variation among the five crystallographic duplexes and the solution structure. The G.U pairs in the five duplexes of the crystal form two direct hydrogen bonds and are stabilized by water molecules that bridge between the base of guanine (N2) and the sugar (O2') of uracil. This contrasts with the NMR structure in which only one direct hydrogen bond is observed for the G.U pairs. The reduced stability of the r(CGUG)2 motif relative to the r(GGUC)2 motif may be explained by the lack of stacking of the uracil bases between the Watson-Crick and G.U pairs as observed in the crystal structure.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Jang SB,Hung LW,Jeong MS,Holbrook EL,Chen X,Turner DH,Holbrook SRdoi
10.1529/biophysj.106.081018subject
Has Abstractpub_date
2006-06-15 00:00:00pages
4530-7issue
12eissn
0006-3495issn
1542-0086pii
biophysj.106.081018journal_volume
90pub_type
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