Mechanistic Insights into the Modulation of Voltage-Gated Ion Channels by Inhalational Anesthetics.

abstract：:Light adaptation in cones was characterized by measuring the changes in temporal frequency responses to sinusoidal modulation of light around various mean levels spanning a range of four log units. We have shown previously that some aspects of cone adaptation behavior can be accounted for by a biochemical kinetic mode...

journal_title：Biophysical journal

authors： Tranchina D,Sneyd J,Cadenas ID

更新日期：1991-07-01 00:00:00

abstract：:Chinese hamster ovary cells were irradiated with 100-5,000 rads of X-rays and inhibition of the initiation of replicons after irradiation was demonstrated by analyzing nascent DNA sedimented in alkaline sucrose gradients. The renaturation kinetics of DNA synthesized during 60 min of incubation after irradiation was co...

journal_title：Biophysical journal

authors： Mattern MR,Painter RB

更新日期：1977-08-01 00:00:00

abstract：:The design and implementation of a high-speed, random-access, laser-scanning fluorescence microscope configured to record fast physiological signals from small neuronal structures with high spatiotemporal resolution is presented. The laser-scanning capability of this nonimaging microscope is provided by two orthogonal...

journal_title：Biophysical journal

authors： Bullen A,Patel SS,Saggau P

更新日期：1997-07-01 00:00:00

abstract：:We demonstrate the first, to our knowledge, integration of stimulated emission depletion (STED) with selective plane illumination microscopy (SPIM). Using this method, we were able to obtain up to 60% improvements in axial resolution with lateral resolution enhancements in control samples and zebrafish embryos. The in...

journal_title：Biophysical journal

authors： Friedrich M,Gan Q,Ermolayev V,Harms GS

更新日期：2011-04-20 00:00:00

abstract：:The crystal structure of the RNA octamer, 5'-GGCGUGCC-3' has been determined from x-ray diffraction data to 1.5 angstroms resolution. In the crystal, this oligonucleotide forms five self-complementary double-helices in the asymmetric unit. Tandem 5'GU/3'UG basepairs comprise an internal loop in the middle of each dupl...

journal_title：Biophysical journal

authors： Jang SB,Hung LW,Jeong MS,Holbrook EL,Chen X,Turner DH,Holbrook SR

更新日期：2006-06-15 00:00:00

abstract：:Members of the epidermal growth factor receptor family play important roles in various cellular processes, both in physiological and in pathological conditions. Dimerization and autophosphorylation of these receptor tyrosine kinases are key events of signal transduction. Details of the molecular events of the signalin...

journal_title：Biophysical journal

authors： Bagossi P,Horváth G,Vereb G,Szöllösi J,Tözsér J

更新日期：2005-02-01 00:00:00

abstract：:Ion conduction across the cellular membrane requires the simultaneous opening of activation and inactivation gates of the K+ channel pore. The bacterial KcsA channel has served as a powerful system for dissecting the structural changes that are related to four major functional states associated with K+ gating. Yet, th...

journal_title：Biophysical journal

authors： van der Cruijsen EA,Prokofyev AV,Pongs O,Baldus M

更新日期：2017-01-10 00:00:00

abstract：:Sickle hemoglobin forms long, multistranded polymers that account for the pathophysiology of the disease. The molecules in these polymers make significant contacts along the polymer axis (i.e., axial contacts) as well as making diagonally directed contacts (i.e., lateral contacts). The axial contacts do not engage the...

journal_title：Biophysical journal

authors： Wang Y,Ferrone FA

更新日期：2013-11-05 00:00:00

abstract：:Receptor activity modifying proteins (RAMPs) are a family of single-pass transmembrane proteins that dimerize with G-protein-coupled receptors. They may alter the ligand recognition properties of the receptors (particularly for the calcitonin receptor-like receptor, CLR). Very little structural information is availabl...

journal_title：Biophysical journal

authors： Simms J,Hay DL,Wheatley M,Poyner DR

更新日期：2006-07-15 00:00:00

abstract：:Polarized photolysis experiments have been performed on the carbon monoxide complex of myoglobin to assess the effects of photoselection on the kinetics of ligand rebinding and to investigate the reorientational dynamics of the heme plane. The results are analyzed in terms of the optical theory developed in the preced...

journal_title：Biophysical journal

authors： Ansari A,Jones CM,Henry ER,Hofrichter J,Eaton WA

更新日期：1993-03-01 00:00:00

abstract：:We have developed a method of performing near-field fluorescence correlation spectroscopy via an array of planarized circular apertures of 50 nm diameter. This technique provides 1 μs and 60 nm resolution on proximal samples, including live cells, without incorporating a scanning probe or pulsed lasers or requiring pe...

journal_title：Biophysical journal

authors： Kelly CV,Baird BA,Craighead HG

更新日期：2011-04-06 00:00:00

abstract：:An understanding of the structure-dynamics relationship is essential for understanding how a protein works. Prior research has shown that the activity of a protein correlates with intradomain dynamics occurring at picosecond to millisecond timescales. However, the correlation between interdomain dynamics and the funct...

journal_title：Biophysical journal

authors： Kumar L,Nash A,Harms C,Planas-Iglesias J,Wright D,Klein-Seetharaman J,Sarkar SK

更新日期：2020-07-21 00:00:00

abstract：:We present a two-component coarse-grained molecular-dynamics model for simulating the erythrocyte membrane. The proposed model possesses the key feature of combing the lipid bilayer and the erythrocyte cytoskeleton, thus showing both the fluidic behavior of the lipid bilayer and the elastic properties of the erythrocy...

journal_title：Biophysical journal

authors： Li H,Lykotrafitis G

更新日期：2012-01-04 00:00:00

abstract：:Monolayers prepared from myelin conserve all the compositional complexity of the natural membrane when spread at the air-water interface. They show a complex pressure-dependent surface pattern that, on compression, changes from the coexistence of two liquid phases to a viscous fractal phase embedded in a liquid phase....

journal_title：Biophysical journal

authors： Rosetti CM,Maggio B

更新日期：2007-12-15 00:00:00

abstract：:DNA represents a promising therapeutic and prophylactic macromolecule in treating genetic diseases, infectious diseases and cancers. The therapeutic potential of DNA is directly related to how DNA transports within the targeted tissue. In this study, fluorescence photobleaching recovery was used to examine the diffusi...

journal_title：Biophysical journal

authors： Shen H,Hu Y,Saltzman WM

更新日期：2006-07-15 00:00:00

abstract：:The photocycle of salinarum halorhodopsin was investigated in the presence of azide. The azide binds to the halorhodopsin with 150 mM binding constant in the absence of chloride and with 250 mM binding constant in the presence of 1 M chloride. We demonstrate that the azide-binding site is different from that of chlori...

journal_title：Biophysical journal

authors： Lakatos M,Groma GI,Ganea C,Lanyi JK,Váró G

更新日期：2002-04-01 00:00:00

abstract：:Electrorotation of fixed red blood cells has been investigated in the frequency range between 16 Hz and 30 MHz. The rotation was studied as a function of electrolyte conductivity and surface charge density. Between 16 Hz and 1 kHz, fixed red blood cells undergo cofield rotation. The maximum of cofield rotation occurs ...

journal_title：Biophysical journal

authors： Georgieva R,Neu B,Shilov VM,Knippel E,Budde A,Latza R,Donath E,Kiesewetter H,Bäumler H

更新日期：1998-04-01 00:00:00

abstract：:The genetic map of T4 (and T2) bacteriophage is circular but the DNA molecule that is liberated by phenol extraction is a linear duplex of polynucleotide chains. If the genetic map is related to the physical structure of the DNA molecule, the problem arises as to how a linear molecule can give rise to a circular map. ...

journal_title：Biophysical journal

authors： THOMAS CA Jr,RUBENSTEIN I

更新日期：1964-03-01 00:00:00

abstract：:Recently various pathways of human telomere (ht) DNA folding into G-quadruplexes and of ligand binding to these structures have been proposed. However, the key issue as to the nature of forces driving the folding and recognition processes remains unanswered. In this study, structural changes of 22-mer ht-DNA fragment ...

journal_title：Biophysical journal

authors： Bončina M,Hamon F,Islam B,Teulade-Fichou MP,Vesnaver G,Haider S,Lah J

更新日期：2015-06-16 00:00:00

abstract：:To elucidate effects of electrostatic interactions resulting from surface charges on structures and phase stability of cubic phases of lipid membranes, membranes of 1-monoolein (MO) and dioleoylphosphatidic acid (DOPA) (DOPA/MO membrane) mixtures have been investigated by small-angle x-ray scattering method. As increa...

journal_title：Biophysical journal

authors： Li SJ,Yamashita Y,Yamazaki M

更新日期：2001-08-01 00:00:00

abstract：:Previous studies have demonstrated that the carboxyl terminus of the gap junction protein Cx43 (Cx43CT) can act as an independent, regulatory domain that modulates intercellular communication in response to appropriate chemical stimuli. Here, we have used NMR, chemical cross-linking, and analytical ultracentrifugation...

journal_title：Biophysical journal

authors： Sorgen PL,Duffy HS,Spray DC,Delmar M

更新日期：2004-07-01 00:00:00

abstract：:Antibodies are key tools in biomedical research and medicine. Their binding properties are classically measured in solution and characterized by an affinity. However, in physiological conditions, antibodies can bridge an immune effector cell and an antigen-presenting cell, implying that mechanical forces may apply to ...

journal_title：Biophysical journal

authors： González C,Chames P,Kerfelec B,Baty D,Robert P,Limozin L

更新日期：2019-04-23 00:00:00

abstract：:A method for fabricating biomimetic surfaces from intact cell membranes is described. A monolayer of alkanethiol on gold is covered by a second layer derived from the components of erythrocyte membranes either by self-assembly or by Langmuir-Blodgett methods. The resulting asymmetric hybrid layer was characterized by ...

journal_title：Biophysical journal

authors： Rao NM,Plant AL,Silin V,Wight S,Hui SW

更新日期：1997-12-01 00:00:00

abstract：:The antifungal polyene antibiotic amphotericin B forms channels in lipid membranes that are permeable to ions, water, and nonelectrolytes. Anion, cation, and ion pair coordination in the water-filled pore of the "barrel" unit of the channels was studied by molecular dynamics simulations. Unlike the case of the gramici...

journal_title：Biophysical journal

authors： Khutorsky V

更新日期：1996-12-01 00:00:00

abstract：:Low-power continuous-wave laser radiation is used to form a very stable microbubble at the end of a specially etched and metalized optical fiber probe. We demonstrate that the microbubble, which is firmly attached to the fiber probe, can be used to benignly trap and manipulate living swine sperm cells as well as human...

journal_title：Biophysical journal

authors： Diop M,Taylor R

更新日期：2006-05-15 00:00:00

abstract：:The aim of this study was to gain insight into how interactions between proteins and lipids in membranes are sensed at the protein-lipid interface. As a probe to analyze this interface, we used deuterium-labeled acyl chains that were covalently linked to a model transmembrane peptide. First, a perdeuterated palmitoyl ...

journal_title：Biophysical journal

authors： Nyholm TK,van Duyl B,Rijkers DT,Liskamp RM,Killian JA

更新日期：2011-10-19 00:00:00

abstract：:The ionic strength dependence of the electron self-exchange rate constants of cytochromes c, c551, and b5 has been analyzed in terms of a monopole-dipole formalism (van Leeuwen, J.W. 1983. Biochim. Biophys. Acta. 743:408-421). The dipole moments of the reduced and oxidized forms of Ps. aeruginosa cytochrome c551 are 1...

journal_title：Biophysical journal

authors： Dixon DW,Hong X,Woehler SE

更新日期：1989-08-01 00:00:00

abstract：:Under various physiological and nonphysiological conditions, the intramembranous particles, as seen by freeze-fracture electron microscopy, may be in various degrees of aggregation. To compare various schemes for the measurement of the degree of aggregation, a computer program has been used to generate simulated aggre...

journal_title：Biophysical journal

authors： Donnell JT 2nd,Finegold LX

更新日期：1981-09-01 00:00:00

abstract：:The elastic theory of flexural waves in thin rods accurately predicts the velocity of flagellar bending waves over a wide range of viscosities. This shows that flagella behave as a purely mechanical system for the transmission of these waves. An evaluation of the total bending moment reveals that this moment occurs in...

journal_title：Biophysical journal

authors： Rikmenspoel R

更新日期：1971-05-01 00:00:00

abstract：:Fiber orientation and ventricular geometry are incorporated in a mathematical model for ejection fraction of the human left ventricle. The inadequacy of circularly oriented or "constrictor" fibers to explain high ejection fractions of cylindrical, spherical, or ellipsoidal reference ventricles is demonstrated. A class...

journal_title：Biophysical journal

authors： Sallin EA

更新日期：1969-07-01 00:00:00