Testing of aggregation measurement techniques for intramembranous particles.

abstract：:The composition and electrolyte concentration of the aqueous bathing environment have important consequences for many biological processes and can profoundly affect the behavior of biomolecules. Nevertheless, because of computational limitations, many molecular simulations of biophysical systems can be performed only ...

journal_title：Biophysical journal

authors： Zavadlav J,Sablić J,Podgornik R,Praprotnik M

更新日期：2018-05-22 00:00:00

abstract：:The interpretation of phi-values has led to an understanding of the folding transition state ensemble of a variety of proteins. Although the main guidelines and equations for calculating phi are well established, there remains some controversy about the quality of the numerical values obtained. By analyzing a complete...

journal_title：Biophysical journal

authors： Cobos ES,Candel AM,Martinez JC

更新日期：2008-06-01 00:00:00

abstract：:The interactions between vitamin-A and beta-lactoglobulin have been investigated. We have found that two different complexes can be formed: one involving vitamin-A, and one involving a derivative of vitamin-A that most probably has a retro-beta-ionylidene structure. Room temperature absorption spectra for these comple...

journal_title：Biophysical journal

authors： Hemley R,Kohler BE,Siviski P

更新日期：1979-12-01 00:00:00

abstract：:Formation of the inverted hexagonal (H(II)) phase from the lamellar (L(alpha)) phase of bovine brain-extracted phosphatidylcholine (BBPC) and phosphatidylethanolamine (BBPE) was investigated using 31P-NMR with or without cholesterol. When the ratio of BBPC to BBPE was 1:1, the H(II) formation was observed in the prese...

journal_title：Biophysical journal

authors： Hayakawa E,Naganuma M,Mukasa K,Shimozawa T,Araiso T

更新日期：1998-02-01 00:00:00

abstract：:In the photoactive yellow protein, PYP, both Glu46 and Tyr42 form hydrogen bonds to the phenolic OH group of the p-hydroxycinnamoyl chromophore. Previous work on replacement of the carboxyl group of Glu46 by an amide group (Glu46Gln) has shown that changing the nature of this hydrogen bond has a minimal effect on the ...

journal_title：Biophysical journal

authors： Devanathan S,Lin S,Cusanovich MA,Woodbury N,Tollin G

更新日期：2001-10-01 00:00:00

abstract：:Influenza A virus M2 protein is known to form acid-activated, proton-selective, amantadine-sensitive channels. We directly measured proton uptake in vesicles containing reconstituted M2 by monitoring external pH after addition of valinomycin to vesicles with 100-fold-diluted external [K(+)]. External pH typically incr...

journal_title：Biophysical journal

authors： Moffat JC,Vijayvergiya V,Gao PF,Cross TA,Woodbury DJ,Busath DD

更新日期：2008-01-15 00:00:00

abstract：:It is known that the action of general anesthetics is proportional to their partition coefficient in lipid membranes (Meyer-Overton rule). This solubility is, however, directly related to the depression of the temperature of the melting transition found close to body temperature in biomembranes. We propose a thermodyn...

journal_title：Biophysical journal

authors： Heimburg T,Jackson AD

更新日期：2007-05-01 00:00:00

abstract：:Articular cartilage is the connective tissue that lines joints and provides a smooth surface for joint motion. Because cartilage is avascular, molecular transport occurs primarily via diffusion or convection, and cartilage matrix structure and composition may affect diffusive transport. Because of the inhomogeneous co...

journal_title：Biophysical journal

authors： Leddy HA,Guilak F

更新日期：2008-11-15 00:00:00

abstract：:L-type (alpha(1C)) calcium channels inactivate rapidly in response to localized elevation of intracellular Ca(2+), providing negative Ca(2+) feedback in a diverse array of biological contexts. The dominant Ca(2+) sensor for such Ca(2+)-dependent inactivation has recently been identified as calmodulin, which appears to...

journal_title：Biophysical journal

authors： Peterson BZ,Lee JS,Mulle JG,Wang Y,de Leon M,Yue DT

更新日期：2000-04-01 00:00:00

abstract：:The partition coefficients (K(P)) of a series of single-chain and double-chain fluorescent amphiphiles, between solid ordered (P(beta') and L(beta)) and liquid disordered (L(alpha) of the type l(d)) lipid phases coexisting in the same lipid bilayer, was studied using steady-state fluorescence emission anisotropy. The ...

journal_title：Biophysical journal

authors： Mesquita RM,Melo E,Thompson TE,Vaz WL

更新日期：2000-06-01 00:00:00

abstract：:Single-particle tracking is an important technique in the life sciences to understand the kinetics of biomolecules. The analysis of apparent diffusion coefficients in vivo, for example, enables researchers to determine whether biomolecules are moving alone, as part of a larger complex, or are bound to large cellular c...

journal_title：Biophysical journal

authors： Vink JNA,Brouns SJJ,Hohlbein J

更新日期：2020-11-17 00:00:00

abstract：:The quantitation of human granulocyte movement using a stochastic differential equation is described. The method has the potential to distinguish both positive and negative chemotaxis. Analysis and information concerning cell movements can be obtained for any point in time and distance for the duration of the experime...

journal_title：Biophysical journal

authors： Boyarsky A,Noble PB,Peterson SC

更新日期：1976-03-01 00:00:00

abstract：:Investigations into the spatiotemporal dynamics of DNA repair using live-cell imaging are aided by the ability to generate well defined regions of ultravioletlike photolesions in an optical microscope. We demonstrate that multiphoton excitation of DNA in live cells with visible femtosecond pulses produces thymine cycl...

journal_title：Biophysical journal

authors： Daddysman MK,Fecko CJ

更新日期：2011-11-02 00:00:00

abstract：:Solid phase amplification (SPA), a new method to amplify DNA, is characterized by the use of surface-bound primers. This limits the amplification to two-dimensional surfaces and therefore allows the easy parallelization of DNA amplification in a single system. SPA leads to the formation of small but dense DNA brushes,...

journal_title：Biophysical journal

authors： Mercier JF,Slater GW

更新日期：2005-07-01 00:00:00

abstract：:Cryo-electron microscopy is rapidly emerging as a powerful technique to determine the structures of complex macromolecular systems elusive to other techniques. Because many of these systems are highly dynamical, characterizing their movements is also a crucial step to unravel their biological functions. To achieve thi...

journal_title：Biophysical journal

authors： Bonomi M,Pellarin R,Vendruscolo M

更新日期：2018-04-10 00:00:00

abstract：:The voltage dependence of light-induced proton pumping was studied with bacteriorhodopsin (bR) from Halobacterium salinarum, expressed in the plasma membrane of oocytes from Xenopus laevis in the range -160 mV to +60 mV at different light intensities. Depending on the applied field, the quenching effect by blue light,...

journal_title：Biophysical journal

authors： Nagel G,Kelety B,Möckel B,Büldt G,Bamberg E

更新日期：1998-01-01 00:00:00

abstract：:Potassium accumulation associated with outward membrane potassium current was investigated experimentally in myelinated fibers and analyzed in terms of two models-three-compartment and diffusion in an unstirred layer. In the myelinated fibers, as in squid giant axons, the three-compartment model satisfactorily describ...

journal_title：Biophysical journal

authors： Moran N,Palti Y,Levitan E,Stämpfli R

更新日期：1980-12-01 00:00:00

abstract：:The parameters of the immune response dynamics are usually estimated by the use of deterministic ordinary differential equations that relate data trends to parameter values. Since the physical basis of the response is stochastic, we are investigating the intensity of the data fluctuations resulting from the intrinsic ...

journal_title：Biophysical journal

authors： Milutinovic D,De Boer RJ

更新日期：2007-05-15 00:00:00

abstract：:A subsarcolemmal tubular system network (SSTN) has been detected in skeletal muscle fibers by confocal imaging after the removal of the sarcolemma. Here we confirm the existence and resolve the fine architecture and the localization of the SSTN at an unprecedented level of detail by examining extracellularly applied t...

journal_title：Biophysical journal

authors： Jayasinghe ID,Lo HP,Morgan GP,Baddeley D,Parton RG,Soeller C,Launikonis BS

更新日期：2013-06-04 00:00:00

abstract：:The effects of myosin regulatory light chain (RLC) phosphorylation and strain on adenosine diphosphate (ADP) release from cross-bridges in phasic (rabbit bladder (Rbl)) and tonic (femoral artery (Rfa)) smooth muscle were determined by monitoring fluorescence transients of the novel ADP analog, 3'-deac-eda-ADP (deac-ed...

journal_title：Biophysical journal

authors： Khromov AS,Webb MR,Ferenczi MA,Trentham DR,Somlyo AP,Somlyo AV

更新日期：2004-04-01 00:00:00

abstract：:The incompressibility of the lipid bilayer keeps the total surface area of the red cell membrane constant. Local conservation of membrane surface area requires that each surface element of the membrane skeleton keeps its area when its aspect ratio is changed. A change in area would require a flow of lipids past the in...

journal_title：Biophysical journal

authors： Fischer TM

更新日期：1992-02-01 00:00:00

abstract：:Fitting dwell-time distributions with sums of exponentials is widely used to characterize histograms of open- and closed-interval durations recorded from single ion channels, as well as for other physical phenomena. However, it can be difficult to identify the contributing exponential components. Here we extend previo...

journal_title：Biophysical journal

authors： Landowne D,Yuan B,Magleby KL

更新日期：2013-06-04 00:00:00

abstract：:Excitation-contraction coupling in atrial cells is mediated by calcium (Ca) signaling between L-type Ca channels and Ryanodine receptors that occurs mainly at the cell boundary. This unique architecture dictates essential aspects of Ca signaling under both normal and diseased conditions. In this study we apply laser s...

journal_title：Biophysical journal

authors： Shiferaw Y,Aistrup GL,Wasserstrom JA

更新日期：2017-08-08 00:00:00

abstract：:We present a molecular model of a chromatophore vesicle from Rhodobacter sphaeroides. These vesicles are ideal benchmark systems for molecular and systemic simulations, because they have been well studied, they are small, and they are naturally separated from their cellular environment. To set up a photosynthetic chai...

journal_title：Biophysical journal

authors： Geyer T,Helms V

更新日期：2006-08-01 00:00:00

abstract：:We investigated the accessibility to protons and thiol-directed reagents of a cysteine substituted at position 338 in transmembrane segment 6 (TM6) of CFTR to test the hypothesis that T338 resides in the pore. Xenopus oocytes expressing T338C CFTR exhibited pH-dependent changes in gCl and I-V shape that were specific ...

journal_title：Biophysical journal

authors： Liu X,Zhang ZR,Fuller MD,Billingsley J,McCarty NA,Dawson DC

更新日期：2004-12-01 00:00:00

abstract：:The dependence of the fluorescence of catalase upon the concentration of added superoxide dismutase (SOD) indicates that SOD binds to saturable sites on catalase. The affinity of SOD for these sites varies with temperature, and with the concentration of each of three nominally inert polymeric additives--dextran 70, Fi...

journal_title：Biophysical journal

authors： Jiao M,Li HT,Chen J,Minton AP,Liang Y

更新日期：2010-08-04 00:00:00

abstract：:Kinesin and cytoplasmic dynein are microtubule-based motor proteins that actively transport material throughout the cell. Microtubules can intersect at a variety of angles both near the nucleus and at the cell periphery, and the behavior of molecular motors at these intersections has implications for long-range transp...

journal_title：Biophysical journal

authors： Ross JL,Shuman H,Holzbaur EL,Goldman YE

更新日期：2008-04-15 00:00:00

abstract：:Specific amino acid sequence segments have been assigned to locations in the structural map of bacteriorhodopsin using two-dimensional neutron diffraction data and a model building analysis. Models are constructed computationally by building specific regions of the amino acid sequence as alpha helices and then positio...

journal_title：Biophysical journal

authors： Trewhella J,Anderson S,Fox R,Gogol E,Khan S,Engelman D,Zaccai G

更新日期：1983-06-01 00:00:00

abstract：:Using both quasi-elastic light scattering spectroscopy and angular dissymmetry in the intensity of the scattered light, we examined the onset of turbidity for intact calf lenses and for isolated nuclear cytoplasm. In the case of the nuclear cytoplasm these measurements demonstrate the presence of two kinds of scattere...

journal_title：Biophysical journal

authors： Delaye M,Clark JI,Benedek GB

更新日期：1982-03-01 00:00:00

abstract：:Intracellular transport of cargos along microtubules is often complicated by the topology of the underlying filament network. The fundamental building blocks for this complex arrangement are filament intersections. The navigation of cargos across microtubule intersections remains poorly understood. Here, we demonstrat...

journal_title：Biophysical journal

authors： Osunbayo O,Butterfield J,Bergman J,Mershon L,Rodionov V,Vershinin M

更新日期：2015-03-24 00:00:00