Abstract:
:We have developed a method of performing near-field fluorescence correlation spectroscopy via an array of planarized circular apertures of 50 nm diameter. This technique provides 1 μs and 60 nm resolution on proximal samples, including live cells, without incorporating a scanning probe or pulsed lasers or requiring penetration of the sample into the aperture. Millions of apertures are created in an array within a thin film of aluminum on a coverslip and planarized to achieve no height distinction between the apertures and the surrounding metal. Supported lipid bilayers and plasma membranes from live cells adhere to the top of this substrate. We performed fluorescence correlation spectroscopy to demonstrate the sub-diffraction-limited illumination with these devices.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Kelly CV,Baird BA,Craighead HGdoi
10.1016/j.bpj.2011.02.034subject
Has Abstractpub_date
2011-04-06 00:00:00pages
L34-6issue
7eissn
0006-3495issn
1542-0086pii
S0006-3495(11)00252-9journal_volume
100pub_type
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