Evaluation of dynamic features of Escherichia coli 16S ribosomal RNA in homogeneous physiological solution.

abstract：:How do Ca channels conduct Ca ions during the cardiac action potential? We attempt to answer this question by applying a two-microelectrode technique, previously used for Na and K currents, in which we record the patch current and the action potential at the same time (Mazzanti, M., and L. J. DeFelice. 1987. Biophys. ...

journal_title：Biophysical journal

authors： Mazzanti M,DeFelice LJ

更新日期：1990-10-01 00:00:00

abstract：:Gene regulation involves a hierarchy of events that extend from specific protein-DNA interactions to the combinatorial assembly of nucleoprotein complexes. The effects of DNA sequence on these processes have typically been studied based either on its quantitative connection with single-domain binding free energies or ...

journal_title：Biophysical journal

authors： Vilar JM

更新日期：2010-10-20 00:00:00

abstract：:Protein kinases are key players in signal transduction pathways where they are crafted into two functional states. In response to growth factor binding stimulus, epidermal growth factor receptor (EGFR), which is physiologically populated in an autoinhibited inactive state, becomes active. Here, we outline a simple all...

journal_title：Biophysical journal

authors： Tsai CJ,Nussinov R

更新日期：2019-07-09 00:00:00

abstract：:Steady-state and time-resolved fluorescence spectroscopy was used to follow the local and global changes in structure and dynamics during chemical and thermal denaturation of unlabeled human serum albumin (HSA) and HSA with an acrylodan moiety bound to Cys34. Acrylodan fluorescence was monitored to obtain information ...

journal_title：Biophysical journal

authors： Flora K,Brennan JD,Baker GA,Doody MA,Bright FV

更新日期：1998-08-01 00:00:00

abstract：:Deposition of amyloid-β (Aβ) fibers in the extracellular matrix of the brain is a ubiquitous feature associated with several neurodegenerative disorders, especially Alzheimer's disease (AD). Although many of the biological aspects that contribute to the formation of Aβ plaques are well addressed at the intra- and inte...

journal_title：Biophysical journal

authors： Hoore M,Khailaie S,Montaseri G,Mitra T,Meyer-Hermann M

更新日期：2020-08-18 00:00:00

abstract：:Nanodiscs are an example of discoidal nanoscale self-assembled lipid/protein particles similar to nascent high-density lipoproteins, which reduce the risk of coronary artery disease. The major protein component of high-density lipoproteins is human apolipoprotein A-I, and the corresponding protein component of Nanodis...

journal_title：Biophysical journal

authors： Li Y,Kijac AZ,Sligar SG,Rienstra CM

更新日期：2006-11-15 00:00:00

abstract：:A model is presented for the steric interaction between a plasma membrane protein and the membrane cytoskeleton in the human erythrocyte. The cytoskeleton is treated as a network of polymer chains attached to a flat bilayer, and the membrane protein is a hemisphere of effective radius R(e) with center on the bilayer e...

journal_title：Biophysical journal

authors： Boal DH,Boey SK

更新日期：1995-08-01 00:00:00

abstract：:To investigate the mechanism of interaction of gramicidin S-like antimicrobial peptides with biological membranes, a series of five decameric cyclic cationic beta-sheet-beta-turn peptides with all possible combinations of aromatic D-amino acids, Cyclo(Val-Lys-Leu-D-Ar1-Pro-Val-Lys-Leu-D-Ar2-Pro) (Ar identical with Phe...

journal_title：Biophysical journal

authors： Jelokhani-Niaraki M,Hodges RS,Meissner JE,Hassenstein UE,Wheaton L

更新日期：2008-10-01 00:00:00

abstract：:A recipe is given for designing theoretical models for donor-acceptor systems in which fluorescence energy transfer and motion takes place simultaneously. This recipe is based on the idea that a system exhibiting both motion and fluorescence energy transfer can be modeled by specifying a number of "states" and the rat...

journal_title：Biophysical journal

authors： van der Meer BW,Raymer MA,Wagoner SL,Hackney RL,Beechem JM,Gratton E

更新日期：1993-04-01 00:00:00

abstract：:Hemoglobin functions as a tetrameric oxygen transport protein, with each subunit containing a heme cofactor. Its denaturation, either in vivo or in vitro, involves autoxidation to methemoglobin, followed by cofactor loss and globin unfolding. We have proposed a global disassembly scheme for human methemoglobin, linkin...

journal_title：Biophysical journal

authors： Samuel PP,White MA,Ou WC,Case DA,Phillips GN Jr,Olson JS

更新日期：2020-03-24 00:00:00

abstract：:Biological membranes are composed of a large number lipid species differing in hydrophobic length, degree of saturation, and charge and size of the headgroup. We now present data on the effect of hydrocarbon chain length of the lipids and headgroup composition on the lateral mobility of the proteins in model membranes...

journal_title：Biophysical journal

authors： Ramadurai S,Duurkens R,Krasnikov VV,Poolman B

更新日期：2010-09-08 00:00:00

abstract：:Calcium channels in neurons mediate a wide variety of essential functions. In addition to contributing to action potential shape, they furnish a substrate that acts as an intracellular second messenger. This study shows that the shape of the neuronal action potential has characteristics that promote long openings of L...

journal_title：Biophysical journal

authors： Mazzanti M,Galli A,Ferroni A

更新日期：1992-10-01 00:00:00

abstract：:A delayed voltage-dependent K+ current endogenous to Xenopus oocytes has been investigated by the voltage-clamp technique. Both activation and inactivation of the K+ current are voltage-dependent processes. The K+ currents were activated when membrane potential was depolarized from a holding potential of -90 to -50 mV...

journal_title：Biophysical journal

authors： Lu L,Montrose-Rafizadeh C,Hwang TC,Guggino WB

更新日期：1990-06-01 00:00:00

abstract：:The plasma membrane of cells has a complex architecture based on the bidimensional liquid-crystalline bilayer arrangement of phospho- and sphingolipids, which in turn embeds several proteins and is connected to the cytoskeleton. Several studies highlight the spatial membrane organization into more ordered (Lo or lipid...

journal_title：Biophysical journal

authors： Abbandonato G,Storti B,Tonazzini I,Stöckl M,Subramaniam V,Montis C,Nifosì R,Cecchini M,Signore G,Bizzarri R

更新日期：2019-02-05 00:00:00

abstract：:Voltage-gated ion channels are crucial for regulation of electric activity of excitable tissues such as nerve cells, and play important roles in many diseases. During activation, the charged S4 segment in the voltage sensor domain translates across a hydrophobic core forming a barrier for the gating charges. This barr...

journal_title：Biophysical journal

authors： Schwaiger CS,Liin SI,Elinder F,Lindahl E

更新日期：2013-01-08 00:00:00

abstract：:Kinesin-1 is an ATP-driven molecular motor that transports cellular cargo along microtubules. At low loads, kinesin-1 almost always steps forward, toward microtubule plus ends, but at higher loads, it can also step backward. Backsteps are usually 8 nm but can be larger. These larger backward events of 16 nm, 24 nm, or...

journal_title：Biophysical journal

authors： Toleikis A,Carter NJ,Cross RA

更新日期：2020-11-17 00:00:00

abstract：:The proposition is made that the red cell membrane is a two-dimensional, incompressible material and a general stress-strain law is developed for finite deformations. In the linear form, the character of such a material is analogous to a two-dimensional Mooney material (e.g., rubber), indicating that the molecular str...

journal_title：Biophysical journal

authors： Evans EA

更新日期：1973-09-01 00:00:00

abstract：:Dim-light vision is mediated by retinal rod cells. Rhodopsin (R), a G-protein-coupled receptor, switches to its active form (R(∗)) in response to absorbing a single photon and activates multiple copies of the G-protein transducin (G) that trigger further downstream reactions of the phototransduction cascade. The class...

journal_title：Biophysical journal

authors： Schöneberg J,Heck M,Hofmann KP,Noé F

更新日期：2014-09-02 00:00:00

abstract：:A new model is proposed to account for the apparent conductance changes of the sodium, or early, channel in nerve fiber membranes. In this model it is assumed that the channels are gated at the interior side of the membrane and are resistively limited at the exterior side by sodium selective barriers of high resistanc...

journal_title：Biophysical journal

authors： Hoyt RC,Strieb JD

更新日期：1971-11-01 00:00:00

abstract：:We expressed rod-type homotetrameric cyclic nucleotide-gated (CNGA1) channels in Xenopus oocytes and studied activation by photolysis-induced jumps of the 3',5'-cyclic guanosine monophosphate (cGMP) concentration and by voltage steps. cGMP jumps to increasing concentrations up to the EC50 value of 46.5 microM decelera...

journal_title：Biophysical journal

authors： Nache V,Kusch J,Hagen V,Benndorf K

更新日期：2006-05-01 00:00:00

abstract：:Intramolecular electron transfer between CuA and heme a in solubilized bacterial (Paracoccus denitrificans) cytochrome c oxidase was investigated by pulse radiolysis. CuA, the initial electron acceptor, was reduced by 1-methylnicotinamide radicals in a diffusion-controlled reaction, as monitored by absorption changes ...

journal_title：Biophysical journal

authors： Farver O,Grell E,Ludwig B,Michel H,Pecht I

更新日期：2006-03-15 00:00:00

abstract：:The insertion of myelin basic protein into microemulsion droplets of sodium bis (2-ethylhexyl) sulfosuccinate (AOT) has been studied by quasi-elastic light scattering. Measurements were made at both low and high molar ratios of water to surfactant, as a function of protein occupancy. The hydrodynamic radii of filled a...

journal_title：Biophysical journal

authors： Chatenay D,Urbach W,Cazabat AM,Vacher M,Waks M

更新日期：1985-12-01 00:00:00

abstract：:Three features appear to characterize steady-state light adaptation in vertebrate cone photoreceptors: (a) the shape of the "log intensity-response" curve at different levels of adaptation is the same, the only change with adaptation is in the position of the point on the curve about which the cones operate; (b) at hi...

journal_title：Biophysical journal

authors： Dawis SM,Purple RL

更新日期：1982-08-01 00:00:00

abstract：:The temporal dynamics of the concentrations of several proteins are tightly regulated, particularly for critical nodes in biological networks such as transcription factors. An important mechanism to control transcription factor levels is through autoregulatory feedback loops where the protein can bind its own promoter...

journal_title：Biophysical journal

authors： Murugan R,Kreiman G

更新日期：2011-09-21 00:00:00

abstract：:Stationary and kinetic experiments were performed on lipid bilayer membranes to study the mechanism of iodine- and bromine-mediated halide transport in detail. The stationary conductance data suggested that four different 1:1 complexes between I2 and Br2 and the halides I- and Br- were responsible for the observed con...

journal_title：Biophysical journal

authors： Klotz KH,Benz R

更新日期：1993-12-01 00:00:00

abstract：:The orientational ordering of beta-carotene and crocetin embedded in lamellar model membranes has been investigated by angle-resolved resonance Raman scattering at a temperature well above the phase transition of the lipid chains. It is shown that the ordering of the carotenoids is dependent on the chemical compositio...

journal_title：Biophysical journal

authors： van de Ven M,Kattenberg M,van Ginkel G,Levine YK

更新日期：1984-06-01 00:00:00

abstract：:The structure and dynamics of soluble misfolded aggregates are poorly understood, despite their importance in protein science and disease. Water-soluble self-associated species that do not become insoluble over time are invaluable tools for high-resolution conformational studies aimed at dissecting the determinants of...

journal_title：Biophysical journal

authors： Rajagopalan S,Kurt N,Cavagnero S

更新日期：2011-02-02 00:00:00

abstract：:The aggregation of amyloid-β (Aβ) peptides plays a crucial role in the etiology of Alzheimer's disease (AD). Recently, it has been reported that an A2T mutation in Aβ can protect against AD. Interestingly, a nonpolar A2V mutation also has been found to offer protection against AD in the heterozygous state, although it...

journal_title：Biophysical journal

authors： Das P,Murray B,Belfort G

更新日期：2015-02-03 00:00:00

abstract：:Crystals of ion-free gramicidin A (P212121: a = 24.61, b = 32.28, c = 32.52) have been investigated using neutron diffraction. A difference analysis of crystals soaked in ethanol/H2O as opposed to ethanol-d6/D2O has led to single isomorphous replacement Fourier projections of the structure at 5-A resolution. The grami...

journal_title：Biophysical journal

authors： Koeppe RE 2nd,Schoenborn BP

更新日期：1984-03-01 00:00:00

abstract：:Molecular dynamics calculations demonstrated the conformational change in the prion protein due to Ala(117)-->Val mutation, which is related to Gerstmann-Sträussler-Sheinker disease, one of the familial prion diseases. Three kinds of model structures of human and mouse prion proteins were examined: (model 1) nuclear m...

journal_title：Biophysical journal

authors： Okimoto N,Yamanaka K,Suenaga A,Hata M,Hoshino T

更新日期：2002-05-01 00:00:00