Lipid Organization in Mixed Lipid Membranes Driven by Intrinsic Curvature Difference.

abstract：:The polar interface of membranes containing phosphatidylglycerol or cholesterol was studied by (2)H nuclear magnetic resonance (NMR) as a function of membrane hydration. The membranes were macroscopically aligned and hydrated with deuterium oxide. Water uptake and membrane annealing was achieved under NMR control, usi...

journal_title：Biophysical journal

authors： Kurze V,Steinbauer B,Huber T,Beyer K

更新日期：2000-05-01 00:00:00

abstract：:We study the stochastic dynamics of growth and shrinkage of single actin filaments taking into account insertion, removal, and ATP hydrolysis of subunits either according to the vectorial mechanism or to the random mechanism. In a previous work, we developed a model for a single actin or microtubule filament where hyd...

journal_title：Biophysical journal

authors： Ranjith P,Mallick K,Joanny JF,Lacoste D

更新日期：2010-04-21 00:00:00

abstract：:Transmembrane osmotic gradients applied on large unilamellar 1-palmitoyl-2-oleoyl-phosphatidylcholine vesicles were used to modulate the potency of melittin to induce leakage. Melittin, an amphipathic peptide, changes the permeability of vesicles, as studied using the release of entrapped calcein, a fluorescent marker...

journal_title：Biophysical journal

authors： Benachir T,Lafleur M

更新日期：1996-02-01 00:00:00

abstract：:Titin is a giant filamentous polypeptide of multidomain construction spanning between the Z- and M-lines of the cardiac muscle sarcomere. Extension of the I-band segment of titin gives rise to a force that underlies part of the diastolic force of cardiac muscle. Titin's force arises from its extensible I-band region, ...

journal_title：Biophysical journal

authors： Trombitás K,Freiburg A,Centner T,Labeit S,Granzier H

更新日期：1999-12-01 00:00:00

abstract：:Both the solution and the oriented film absorption and circular dichroic spectra of the bacteriorhodopsin (bR(568)) and M(412) intermediate of the purple membrane photocycle were compared over the wavelength region 800-183 nm to assess structural changes during this photocycle. The main findings are (a) loss of the ex...

journal_title：Biophysical journal

authors： Draheim JE,Cassim JY

更新日期：1985-04-01 00:00:00

abstract：:Aqueous proline solutions are deceptively simple as they can take on complex roles such as protein chaperones, cryoprotectants, and hydrotropic agents in biological processes. Here, a molecular level picture of proline/water mixtures is developed. Car-Parrinello ab initio molecular dynamics (CPAIMD) simulations of aqu...

journal_title：Biophysical journal

authors： Troitzsch RZ,Tulip PR,Crain J,Martyna GJ

更新日期：2008-12-01 00:00:00

abstract：:Biophysical understanding of membrane domains requires accurate knowledge of their structural details and elasticity. We report on a global small angle x-ray scattering data analysis technique for coexisting liquid-ordered (Lo) and liquid-disordered (Ld) domains in fully hydrated multilamellar vesicles. This enabled t...

journal_title：Biophysical journal

authors： Heftberger P,Kollmitzer B,Rieder AA,Amenitsch H,Pabst G

更新日期：2015-02-17 00:00:00

abstract：:We have studied the photoinduced volume changes, energetics, and kinetics in the early steps of the bacteriorhodopsin (BR) photocycle with pulsed, time-resolved photoacoustics. Our data show that there are two volume changes. The fast volume change ( < or = 200 ns) is an expansion (2.5 +/- 0.3 A3/molecule) and is obse...

journal_title：Biophysical journal

authors： Zhang D,Mauzerall D

更新日期：1996-07-01 00:00:00

abstract：:P188, a triblock copolymer of the form poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) helps seal electroporated cell membranes, arresting the leakage of intracellular materials from the damaged cells. To explore the nature of the interaction between P188 and cell membranes, we have constructed a model...

journal_title：Biophysical journal

authors： Maskarinec SA,Hannig J,Lee RC,Lee KY

更新日期：2002-03-01 00:00:00

abstract：:A long-standing question about membrane structure and function is the degree to which the physical properties of the inner and outer leaflets of a bilayer are coupled to one another. Using our recently developed methods to prepare asymmetric vesicles, coupling was investigated for vesicles containing phosphatidylcholi...

journal_title：Biophysical journal

authors： Chiantia S,London E

更新日期：2012-12-05 00:00:00

abstract：:Magic-angle spinning nuclear magnetic resonance is well suited for the study of membrane proteins in the nativelike lipid environment. However, the natural cellular membrane is invariably more complex than the proteoliposomes most often used for solid-state NMR (SSNMR) studies, and differences may affect the structure...

journal_title：Biophysical journal

authors： Ward ME,Wang S,Munro R,Ritz E,Hung I,Gor'kov PL,Jiang Y,Liang H,Brown LS,Ladizhansky V

更新日期：2015-04-07 00:00:00

abstract：:In cardiac tissue, during partial blockade of the membrane sodium channels, or at high frequencies of excitation, inexcitable obstacles with sharp edges may destabilize the propagation of electrical excitation waves, causing the formation of self-sustained vortices and turbulent cardiac electrical activity. The format...

journal_title：Biophysical journal

authors： Cabo C,Pertsov AM,Davidenko JM,Baxter WT,Gray RA,Jalife J

更新日期：1996-03-01 00:00:00

abstract：:The structure of a membrane-embedded alpha-helical reference protein, the M13 major coat protein, is characterized under different conditions of hydrophobic mismatch using fluorescence resonance energy transfer in combination with high-throughput mutagenesis. We show that the structure is similar in both thin (14:1) a...

journal_title：Biophysical journal

authors： Vos WL,Schor M,Nazarov PV,Koehorst RB,Spruijt RB,Hemminga MA

更新日期：2007-11-15 00:00:00

abstract：:Cationic lipid-DNA (CL-DNA) complexes are abundantly used in nonviral gene therapy clinical applications. Surface functionality is the next step in developing these complexes as competent, target-specific gene carriers. Poly(ethylene glycol) (PEG) is the natural choice to serve as a protective coat or act as a tether ...

journal_title：Biophysical journal

authors： Martin-Herranz A,Ahmad A,Evans HM,Ewert K,Schulze U,Safinya CR

更新日期：2004-02-01 00:00:00

abstract：:We present the measurement of the force required to rupture a single protein-sugar bond using a methodology that provides selective discrimination between specific and nonspecific binding events and helps verify the presence of a single functional molecule on the atomic force microscopy tip. In particular, the interac...

journal_title：Biophysical journal

authors： Ratto TV,Langry KC,Rudd RE,Balhorn RL,Allen MJ,McElfresh MW

更新日期：2004-04-01 00:00:00

abstract：:Although the structure, function, conformational dynamics, and controlled thermodynamics of proteins are manifested by their corresponding amino acid sequences, the natural rules for molecular design and their corresponding interplay remain obscure. In this study, we focused on the role of internal cavities of protein...

journal_title：Biophysical journal

authors： Maeno A,Sindhikara D,Hirata F,Otten R,Dahlquist FW,Yokoyama S,Akasaka K,Mulder FA,Kitahara R

更新日期：2015-01-06 00:00:00

abstract：:The separation method, flow field-flow fractionation (flow FFF), is coupled on-line with multiangle laser light scattering (MALLS) for simultaneous measurement of the size and concentration of vesicles eluting continuously from the fractionator. These size and concentration data, gathered as a function of elution time...

journal_title：Biophysical journal

authors： Korgel BA,van Zanten JH,Monbouquette HG

更新日期：1998-06-01 00:00:00

abstract：:The influence of mass transport on ligand binding to receptor immobilized in a polymer matrix, as detected with an evanescent wave biosensor, was investigated. A one-dimensional computer model for the mass transport of ligand between the bulk solution and the polymer gel and within the gel was employed, and the influe...

journal_title：Biophysical journal

authors： Schuck P

更新日期：1996-03-01 00:00:00

abstract：:With a view to determine the configuration and regularity of plectonemically supercoiled DNA, we have measured the small angle neutron scattering from pUC18 plasmid in saline solutions. Furthermore, we have derived the mathematical expression for the single chain scattering function (form factor) of a superhelical str...

journal_title：Biophysical journal

authors： Zakharova SS,Jesse W,Backendorf C,Egelhaaf SU,Lapp A,van der Maarel JR

更新日期：2002-08-01 00:00:00

abstract：:The diffusion of plastoquinol and its binding to the cytochrome bf complex, which occurs during linear photosynthetic electron transport and is analogous to reaction sequences found in most energy-converting membranes, has been studied in intact thylakoid membranes. The flash-induced electron transfer between the late...

journal_title：Biophysical journal

authors： Mitchell R,Spillmann A,Haehnel W

更新日期：1990-10-01 00:00:00

abstract：:The parameters of the immune response dynamics are usually estimated by the use of deterministic ordinary differential equations that relate data trends to parameter values. Since the physical basis of the response is stochastic, we are investigating the intensity of the data fluctuations resulting from the intrinsic ...

journal_title：Biophysical journal

authors： Milutinovic D,De Boer RJ

更新日期：2007-05-15 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopy was used to monitor secondary structural changes associated with binding of bovine prothrombin and prothrombin fragment 1 to acidic lipid membranes. Prothrombin and prothrombin fragment 1 were examined under four different conditions: in the presence of (a) Na2EDTA, (b) 5...

journal_title：Biophysical journal

authors： Wu JR,Lentz BR

更新日期：1991-07-01 00:00:00

abstract：:Fluorescence lifetimes of dimeric rabbit muscle creatine kinase specifically dansylated at both active sites and the homologous monomeric lobster muscle arginine kinase singly dansylated were determined using phase-modulation methods with global analysis of overdetermined data sets. For both proteins, the data is adeq...

journal_title：Biophysical journal

authors： Grossman SH

更新日期：1991-03-01 00:00:00

abstract：:The clustering of ryanodine receptors (RyR2) into functional Ca2+ release units is central to current models for cardiac excitation-contraction (E-C) coupling. Using immunolabeling and confocal microscopy, we have analyzed the distribution of RyR2 clusters in rat and ventricular atrial myocytes. The resolution of the ...

journal_title：Biophysical journal

authors： Chen-Izu Y,McCulle SL,Ward CW,Soeller C,Allen BM,Rabang C,Cannell MB,Balke CW,Izu LT

更新日期：2006-07-01 00:00:00

abstract：:Membrane proteins diffuse within the membrane, form oligomers and supramolecular assemblies. Using high-speed atomic force microscopy, we present direct experimental measure of an in-membrane-plane interaction potential between membrane proteins. In purple membranes, ATP-synthase c-rings formed dimers that temporarily...

journal_title：Biophysical journal

authors： Casuso I,Sens P,Rico F,Scheuring S

更新日期：2010-10-06 00:00:00

abstract：:The calyx of Held is a giant nerve terminal that forms a glutamatergic synapse in the auditory pathway. Due to its large size, it offers a number of advantages for biophysical studies, including voltage-clamp of both pre- and postsynaptic compartments and the loading with indicator dyes and caged compounds. Three aspe...

journal_title：Biophysical journal

authors： Neher E

更新日期：2017-01-24 00:00:00

abstract：:Impact loading of articular cartilage causes extensive chondrocyte death. Cell membranes have a limited elastic range of 3-4% strain but are protected from direct stretch during physiological loading by their membrane reservoir, an intricate pattern of membrane folds. Using a finite-element model, we suggested previou...

journal_title：Biophysical journal

authors： Moo EK,Amrein M,Epstein M,Duvall M,Abu Osman NA,Pingguan-Murphy B,Herzog W

更新日期：2013-10-01 00:00:00

abstract：:It has been hypothesized, from earlier studies on single-tether extraction from individual leukocytes and human umbilical vein endothelial cells, that during rolling of leukocytes on the endothelium, simultaneous extraction of membrane nanotubes (tethers) occurs, resulting in enhancement of the force decrease on the a...

journal_title：Biophysical journal

authors： Girdhar G,Shao JY

更新日期：2007-12-01 00:00:00

abstract：:A 250 ps molecular dynamics simulation of the dimyristoylphosphatidylcholine (DMPC)-based lipid bilayer, including explicit water molecules, is reported. The solvent environment of the head groups and other structural properties of the bilayer have been analyzed and compared with experimental results as well as our pr...

journal_title：Biophysical journal

authors： Damodaran KV,Merz KM Jr

更新日期：1994-04-01 00:00:00

abstract：:Previously (Hines, M., and J.J. Blum 1983, Biophys. J., 41:67-79), a method was developed that allowed one to compute curvature and twist for a three-dimensional sliding filament model. In that formalism it was difficult to specify the shear and bending moments arising from moment-bearing interfilament links such as f...

journal_title：Biophysical journal

authors： Hines M,Blum JJ

更新日期：1984-11-01 00:00:00