Abstract:
:Membrane proteins diffuse within the membrane, form oligomers and supramolecular assemblies. Using high-speed atomic force microscopy, we present direct experimental measure of an in-membrane-plane interaction potential between membrane proteins. In purple membranes, ATP-synthase c-rings formed dimers that temporarily dissociated. C-ring dimers revealed subdiffusive motion, while dissociated monomers diffused freely. C-rings center-to-center distance probability distribution allowed the calculation and modeling of an in-membrane-plane energy landscape that presented repulsion at 80 Å, most stable dimer association at 103 Å (-3.5 k(B)T strength), and dissociation at 125 Å (-1 k(B)T strength). This first experimental data of nonlabeled membrane protein diffusion and the corresponding in-membrane-plane interaction energy landscape characterized membrane protein interaction with an attractive range of several k(B)T that reaches to a radius of ∼50 Å within the membrane plane.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Casuso I,Sens P,Rico F,Scheuring Sdoi
10.1016/j.bpj.2010.07.028subject
Has Abstractpub_date
2010-10-06 00:00:00pages
L47-9issue
7eissn
0006-3495issn
1542-0086pii
S0006-3495(10)00900-8journal_volume
99pub_type
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