Stability of the heme environment of the nitric oxide synthase from Staphylococcus aureus in the absence of pterin cofactor.

abstract：:Axonal beading, or the formation of a series of swellings along the axon, and retraction are commonly observed shape transformations that precede axonal atrophy in Alzheimer's disease, Parkinson's disease, and other neurodegenerative conditions. The mechanisms driving these morphological transformations are poorly und...

journal_title：Biophysical journal

authors： Datar A,Ameeramja J,Bhat A,Srivastava R,Mishra A,Bernal R,Prost J,Callan-Jones A,Pullarkat PA

更新日期：2019-09-03 00:00:00

abstract：:Numerous isotypes of the structural protein tubulin have now been characterized in various organisms and their expression offers a plausible explanation for observed differences affecting microtubule function in vivo. While this is an attractive hypothesis, there are only a handful of studies demonstrating a direct in...

journal_title：Biophysical journal

authors： Rezania V,Azarenko O,Jordan MA,Bolterauer H,Ludueña RF,Huzil JT,Tuszynski JA

更新日期：2008-08-01 00:00:00

abstract：:It is shown that if x(t) is the solution of a second order differential equation, with real negative characteristic roots (not necessarily distinct), which exhibits an extremum at t = T, then T|x(T)|/|A| [UNK] 1/e where A is the area under the x(t) curve. This result is compared to a special case previously derived by...

journal_title：Biophysical journal

authors： HEARON JZ

更新日期：1961-09-01 00:00:00

abstract：:Transmembrane osmotic gradients applied on large unilamellar 1-palmitoyl-2-oleoyl-phosphatidylcholine vesicles were used to modulate the potency of melittin to induce leakage. Melittin, an amphipathic peptide, changes the permeability of vesicles, as studied using the release of entrapped calcein, a fluorescent marker...

journal_title：Biophysical journal

authors： Benachir T,Lafleur M

更新日期：1996-02-01 00:00:00

abstract：:The structure of crotapotin, a protein extracted, from the venom of the Crotalus durissus terrificus, in solution at pH = 1.5, was studied by SAXS. The experimental results yield structural parameter values of the molecular radius of gyration Rg = 13.6 A, volume v = 16.2 x 10(3) A3 A3 and maximal dimension Dmax = 46 A...

journal_title：Biophysical journal

authors： Abrego JR,Craievich AF,Mascarenhas YP,Laure CJ

更新日期：1993-02-01 00:00:00

abstract：:The gelation of sickle hemoglobin includes the formation of spherulitic arrays of polymers, known as polymer domains, which are an intrinsic result of the polymer formation mechanism. We have observed the diffusion of monomers into domains as they form, which substantially increases the total concentration of hemoglob...

journal_title：Biophysical journal

authors： Cho MR,Ferrone FA

更新日期：1990-10-01 00:00:00

abstract：:Phosphatidylinositol-4,5-bisphosphate (PIP2) is an important signaling lipid in eukaryotic cell plasma membranes, playing an essential role in diverse cellular processes. The headgroup of PIP2 is highly negatively charged, and this lipid displays a high critical micellar concentration compared to housekeeping phosphol...

journal_title：Biophysical journal

authors： Shukla S,Jin R,Robustelli J,Zimmerman ZE,Baumgart T

更新日期：2019-09-03 00:00:00

abstract：:Bacterial swimming speed is sometimes known to increase with viscosity. This phenomenon is peculiar to bacterial motion. Berg and Turner (Nature. 278:349-351, 1979) indicated that the phenomenon was caused by a loose, quasi-rigid network formed by polymer molecules that were added to increase viscosity. We mathematica...

journal_title：Biophysical journal

authors： Magariyama Y,Kudo S

更新日期：2002-08-01 00:00:00

abstract：:We have developed new methodology for measuring intracellular pH (pHi) in cultured cell monolayers and epithelia by analyzing the emission spectra of the trapped fluorescent pH probe, 1,4-dihydroxyphthalonitrile (1,4-DHPN). This compound is unique since both its acid and base forms possess different fluorescence emiss...

journal_title：Biophysical journal

authors： Kurtz I,Balaban RS

更新日期：1985-09-01 00:00:00

abstract：:The adhesion of red blood cells (RBCs) to cells or surfaces is of current basic science and clinical interest yet the details governing this process are still being explored. In this study, the effects of nonadsorbing polymers on the adhesion of RBC to albumin-coated glass have been investigated employing interference...

journal_title：Biophysical journal

authors： Zhang ZW,Neu B

更新日期：2009-08-19 00:00:00

abstract：:A computational framework is presented for studying the mechanical response of macromolecules. The method combines a continuum mechanics (CM) model for the mechanical properties of the macromolecule with a continuum electrostatic (CE) treatment of solvation. The molecules are represented by their shape and key physico...

journal_title：Biophysical journal

authors： Ma L,Yethiraj A,Chen X,Cui Q

更新日期：2009-05-06 00:00:00

abstract：:The early events in halorhodopsin after light excitation are studied with picosecond time resolution. Absorption and fluorescence measurements show that the electronically excited state of the incorporated retinal has a lifetime of 5 ps. Within that time a red-shifted photoproduct is formed that remains stable for at ...

journal_title：Biophysical journal

authors： Polland HJ,Franz MA,Zinth W,Kaiser W,Hegemann P,Oesterhelt D

更新日期：1985-01-01 00:00:00

abstract：:The detection of the 1H NMR signal of myoglobin (Mb) in tissue opens an opportunity to examine its cellular diffusion property, which is central to its purported role in facilitating oxygen transport. In perfused myocardium the field-dependent transverse relaxation analysis of the deoxy Mb proximal histidyl NdeltaH in...

journal_title：Biophysical journal

authors： Wang D,Kreutzer U,Chung Y,Jue T

更新日期：1997-11-01 00:00:00

abstract：:A discrete multistate kinetic model for water-wire proton transport is constructed and analyzed using Monte Carlo simulations. In the model, each water molecule can be in one of three states: oxygen lone-pairs pointing leftward, pointing rightward, or protonated (H(3)O(+)). Specific rules for transitions among these s...

journal_title：Biophysical journal

authors： Chou T

更新日期：2004-05-01 00:00:00

abstract：:Resolving the dynamical interplay of proteins and lipids in the live-cell plasma membrane represents a central goal in current cell biology. Superresolution concepts have introduced a means of capturing spatial heterogeneity at a nanoscopic length scale. Similar concepts for detecting dynamical transitions (superresol...

journal_title：Biophysical journal

authors： Ruprecht V,Wieser S,Marguet D,Schütz GJ

更新日期：2011-06-08 00:00:00

abstract：:Viral infection begins with the binding of a virus to a specific target on the surface of the host cell, followed by viral genome delivery into the host and a continuation of the infection process. Before binding occurs, the virus must first find its receptor by a process whose details are largely unknown. We applied ...

journal_title：Biophysical journal

authors： Rothenberg E,Sepúlveda LA,Skinner SO,Zeng L,Selvin PR,Golding I

更新日期：2011-06-22 00:00:00

abstract：:Specific amino acid sequence segments have been assigned to locations in the structural map of bacteriorhodopsin using two-dimensional neutron diffraction data and a model building analysis. Models are constructed computationally by building specific regions of the amino acid sequence as alpha helices and then positio...

journal_title：Biophysical journal

authors： Trewhella J,Anderson S,Fox R,Gogol E,Khan S,Engelman D,Zaccai G

更新日期：1983-06-01 00:00:00

abstract：:In the studies of force-induced conformational transitions of biomolecules, the large timescale difference from experiments presents the challenge of obtaining convergent sampling for molecular dynamics simulations. To circumvent this fundamental problem, an approach combining the replica-exchange method and umbrella ...

journal_title：Biophysical journal

authors： Zeng X,Hu H,Zhou HX,Marszalek PE,Yang W

更新日期：2010-02-17 00:00:00

abstract：:The electrical membrane potential (Vm) is one of the components of the electrochemical potential of protons across the biological membrane (proton motive force), which powers many vital cellular processes. Because Vm also plays a role in signal transduction, measuring it is of great interest. Over the years, a variety...

journal_title：Biophysical journal

authors： Mancini L,Terradot G,Tian T,Pu Y,Li Y,Lo CJ,Bai F,Pilizota T

更新日期：2020-01-07 00:00:00

abstract：:Recent work has introduced a new fluorescent voltage sensor, ASAP1, which can monitor rapid trains of action potentials in cultured neurons. This indicator is based on the Gallus gallus voltage-sensitive phosphatase with the phosphatase domain removed and a circularly permuted GFP placed in the S3-S4 linker. However, ...

journal_title：Biophysical journal

authors： Lee EEL,Bezanilla F

更新日期：2017-11-21 00:00:00

abstract：:Singlet-singlet annihilation experiments have been performed on trimeric and aggregated light-harvesting complex II (LHCII) using picosecond spectroscopy to study spatial equilibration times in LHCII preparations, complementing the large amount of data on spectral equilibration available in literature. The annihilatio...

journal_title：Biophysical journal

authors： Barzda V,Gulbinas V,Kananavicius R,Cervinskas V,van Amerongen H,van Grondelle R,Valkunas L

更新日期：2001-05-01 00:00:00

abstract：:Cellular plasma membranes have domains that are defined, in most cases, by cytoskeletal elements. The outer half of the bilayer may also contain domains that organize glycosylphosphatidylinositol (GPI)-linked proteins. To define outer membrane barriers, we measured the resistive force on membrane bound beads as they w...

journal_title：Biophysical journal

authors： Suzuki K,Sterba RE,Sheetz MP

更新日期：2000-07-01 00:00:00

abstract：:Unsaturated phospholipid is the membrane component that is essential to the dynamic environment needed for biomembrane function. The dependence of the chain-melting transition temperature, T(t), of phospholipid bilayer membranes on the position, n(u), of the cis double bond in the glycerophospholipid sn-2 chain can be...

journal_title：Biophysical journal

authors： Marsh D

更新日期：1999-08-01 00:00:00

abstract：:The solution structures of the N-terminal domains of protein S, a plasma vitamin K-dependent glycoprotein, and its homolog growth arrest specific protein 6 (Gas6) were predicted by molecular dynamics computer simulations. The initial structures were based on the x-ray crystallographic structure of the corresponding re...

journal_title：Biophysical journal

authors： Perera L,Li L,Darden T,Monroe DM,Pedersen LG

更新日期：1997-10-01 00:00:00

abstract：:The shape distributions of normal and hardened human and rabbit erythrocytes and platelets were obtained for edge-on orientations of a few hundred freely rotating cells from analyses of microphotographs obtained similarly as by Ponder(1930, Q. J. Exp. Physiol. 20:29) by phase-contrast microscopy at 800 X magnification...

journal_title：Biophysical journal

authors： Frojmovic MM,Panjwani R

更新日期：1976-09-01 00:00:00

abstract：:We demonstrate a novel method for stretching a long DNA molecule in agarose gel with alternating current (AC) electric fields. The molecular motion of a long DNA (T4 DNA; 165.6 kb) in agarose gel was studied using fluorescence microscopy. The effects of a wide range of field frequencies, field strengths, and gel conce...

journal_title：Biophysical journal

authors： Kaji N,Ueda M,Baba Y

更新日期：2002-01-01 00:00:00

abstract：:Certain standard properties, including spontaneous curvature, are assumed for the membrane of a bilipid vesicle. Then, if there is a mechanism that causes the membrane area to increase, vesicle cleavage is found to occur. ...

journal_title：Biophysical journal

authors： Luke JC,Kaplan JI

更新日期：1979-01-01 00:00:00

abstract：:The membrane protein bacteriorhodopsin (BR) can be kept soluble in its native state for months in the absence of detergent by amphipol (APol) A8-35, an amphiphilic polymer. After an actinic flash, A8-35-complexed BR undergoes a complete photocycle, with kinetics intermediate between that in detergent solution and that...

journal_title：Biophysical journal

authors： Gohon Y,Dahmane T,Ruigrok RW,Schuck P,Charvolin D,Rappaport F,Timmins P,Engelman DM,Tribet C,Popot JL,Ebel C

更新日期：2008-05-01 00:00:00

abstract：:We measured the exocytotic response induced by flash photolysis of caged compounds in isolated mast cells and chromaffin cells. Vesicle fusion was measured by monitoring the cell membrane capacitance. The release of vesicular contents was followed by amperometry. In response to a GTP gamma S stimulus we found that the...

journal_title：Biophysical journal

authors： Oberhauser AF,Robinson IM,Fernandez JM

更新日期：1996-08-01 00:00:00

abstract：:We present a quantitative analysis of conformational changes of the nucleotide-binding subunits, MalK(2), of the maltose ATP-binding cassette importer MalFGK(2) during the transport cycle. Distance changes occurring between selected residues were monitored in the full transporter by site-directed spin-labeling electro...

journal_title：Biophysical journal

authors： Grote M,Bordignon E,Polyhach Y,Jeschke G,Steinhoff HJ,Schneider E

更新日期：2008-09-15 00:00:00